High throughput engineering of genetically encodable fluorescent sensors of intracellular signalling networks

Abstract:: Our ability to extract quantitative information on the signalling and metabolic processes in living organisms lags far behind the advancements in post genomic disciplines. This is due to a lack of appropriate methodologies for monitoring biochemical processes in complex environments. In the proposed program I will use a combination of coupled gene and cell-free protein synthesis to establish a high-throughput method for constructing fluorescent protein sensor molecules. Mimicking native proteins, these molecules will display different spectral characteristics depending on their functional state in the cell. I will apply this technology to decipher signal propagation by the GTPase-controlled interaction networks in living cells.'',
Grant type:: ARC Future Fellowships
Funded by:: Australian Research Council

The University of Queensland