Development of a novel high yield cell-free protein expression system

Abstract:: Although cell-free protein production is faster and more flexible than in vivo expression, its widespread use has been hampered by high cost and relatively low productivity. The aim of this proposal is to develop a novel scalable high-yield eukaryotic expression system. This work is based on the discovery by Prof. Alexandrov's group that the protozoan Leishmania tarentolae can serve as a source of cell-free translation extracts. We will use a multidisciplinary approach to develop cell-free system for rapid recombinant protein production on a large-scale. This work will provide a solution for the main bottleneck in protein research and production and will have profound implications for biotechnological and pharmaceutical industries.
Grant type:: ARC Linkage Projects
Funded by:: Australian Research Council

The University of Queensland