Membrane Proteins within the Mouse Transcriptome- Annotation of their Organisation and Subcellular Localisation (2004–2006)
A major issue in cell biology today is how distinct regions of the cell maintain their unique composition of proteins. The aim of this grant is to identify membrane proteins within the mouse genome and annotate their localisation within the cell. Our multi-discipline effort will combine extensive computational prediction strategies with focused cellular biology experimental determination. The underpinning experimental technology termed reverse transfection arrays allows for high-throughput assessment of cellular phenotype properties for individual proteins.