The University of Queensland

Identification of functionally important autophosphorylation site(s) on ataxia telangiectasia and Rad 3 related (ATR) protein kinase (2006–2008)

Abstract:

The aim of the project is to map the site(s) of autophosphorylation on the protein kinase ATR (ataxia-telangiectasia and rad3-related) with a view to understanding the mechanism of activation of this protein. This will involve immunoprecipitation of ATR, tryptic phosphopeptide mapping and mass spectrometric analysis to identify the autophosphorylation sites. The functional importance of these sites will be determined. This information is of central importance to understanding how ATR is activated to initiate a series of signaling assays in response to DNA replication fork stalling. This will also allow comparison with ATM and DNA-PK phosphoinositide kinases.

Grant type:

ARC Discovery Projects

Funded by:

Australian Research Council