Developing orthogonal synthetic signaling cascades

Abstract:: The bottom-up design of protein-based signaling networks is a key goal of Synthetic Biology. Yet, it remains elusive due to our inability to tailor-make signal transducers and receptors that can be readily compiled into defined signaling networks. This project proposes a generic approach for the construction of molecular switches based on artificially autoinhibited proteases. Using structure-guided design and directed protein evolution, a set of protease-based signal transducers and ligand activated allosteric receptors will be created. The developed components will be used to construct artificial signaling networks in mammalian cells that are orthogonal to the endogenous signaling cascades.
Grant type:: ARC Discovery Projects
Funded by:: Australian Research Council

The University of Queensland