Molecular characterisation of transverse tubule development in skeletal muscle (2016–2019)

Abstract:
The transverse tubule system of skeletal muscle is an extensive plasma membrane domain that allows an action potential transmitted by the nerve to reach the depths of a muscle fibre and trigger calcium release in a synchronised fashion. The T-tubule system is a vital component of the skeletal muscle, frequently disrupted in muscle disease. This is exemplified by aberrant T-tubules and mutations in key T-tubule components in many human myopathies. However, the molecular mechanisms by which the T-tubule system, with its unique morphology and molecular composition, is generated are completely unknown. We will use advanced microscopic techniques together with systematic quantitative analyses to understand the process of T-tubule formation in real-time in a vertebrate model system. Proteomics will be used to identify key T-tubule components. This information will be used to identify early markers of T-tubule formation and then to test their involvement in T-tubule formation in the zebrafish model system and in mice.
Grant type:
NHMRC Project Grant
Researchers:
  • Professorial Research Fellow
    Institute for Molecular Bioscience
    Affiliated Professor
    Centre for Microscopy and Microanalysis
    Faculty of Science
    Affiliate Professor
    School of Biomedical Sciences
    Faculty of Medicine
  • Senior Research Officer
    Institute for Molecular Bioscience
  • Senior Research Fellow
    Institute for Molecular Bioscience
  • Associate Professor
    School of Biomedical Sciences
    Faculty of Medicine
    Affiliate Associate Professor
    Queensland Brain Institute
  • Professorial Research Fellow
    Institute for Molecular Bioscience
    Affiliate Professor
    School of Biomedical Sciences
    Faculty of Medicine
    Exec Director Research Integrity
    Office of the Pro-Vice-Chancellor (Research and International)
  • Principle Research Fellow
    The University of Queensland Diamantina Institute
    Faculty of Medicine
    Affiliated Senior Lecturer
    School of Chemistry and Molecular Biosciences
    Faculty of Science
  • Principal Research Fellow
    Institute for Molecular Bioscience
    Affiliated Senior Research Fellow
    School of Biomedical Sciences
    Faculty of Medicine
Funded by:
National Health and Medical Research Council