Dr Peter Moyle

Lecturer

School of Pharmacy
Faculty of Health and Behavioural Sciences
p.moyle@uq.edu.au
+61 7 334 61869

Overview

Dr Moyle is a lecturer in the UQ School of Pharmacy working in the areas of medicinal chemistry and chemical biology, investigating subunit vaccine development, and outcomes associated with histone post-translational modifications.

The Moyle lab is currently looking for students and postdoctoral fellows with an interest in i) subunit vaccine development; ii) delivery systems for peptide therapeutics; iii) targeted delivery systems; iv) studying the function of posttranslational modifications; v) delivery systems for oligonucleotide therapeutics (e.g. siRNA, shRNA, miRNA). If you are interested in working in any of these areas please feel free to contact Dr Moyle (p.moyle@uq.edu.au). Preference will be given to students and postdoctoral fellows who have their own funding.

Funding may be found from sources including:

1) Australian Postgraduate Award

2) UQ Research Scholarship

3) NHMRC Postgraduate Scholarship (includes $1500/year consumables and $750 for conference travel)

4) Endeavour Scholarships (for overseas students)

5) UQ International Postgraduate Research Scholarships (need outstanding undergraduate performance and research papers)

6) Many countries have their own scholarship schemes to train students overseas, with some also offering research funding. This is well worth looking into.

the following are for Malaysian's:

Malaysia Ministry of Higher Education (MOHE) Scholarships

Maktab Amanah Rakyat (MARA)

Jabatan Perkhidmatan Awam (JPA)

Petronas

the following are for Chinese:

China Scholarship Council (UQ info)

There are also scholarships available from many non-government organisations. Examples include:

1) Cancer Council

2) Heart Foundation

Other sources of scholarship information:

School of Pharmacy

UQ Graduate School

UQ Research and Innovation

Dr Moyle Biosketch:

Dr Moyle received a PhD (Dec 2006) and a Bachelor of Pharmacy (Hons I) (Dec 2001) from The University of Queensland (UQ); graduated from the Pharmaceutical Society of Australia pre-registration pharmacist-training course (Nov 2002); and is registered with the Pharmacy Board of Australia. He currently works as a lecturer in the School of Pharmacy.

Dr Moyle works in the fields of medicinal chemistry and chemical biology, investigating subunit vaccine development, and outcomes associated with histone post-translational modifications. During his PhD, Dr Moyle developed methods that enabled the synthesis of pure, lipid adjuvanted peptide vaccines, using advanced chemical ligation techniques. In addition, the conjugation of mannose to combined prophylactic/therapeutic human papillomavirus type-16 vaccines, to target dendritic cells, was demonstrated to significantly improve vaccine anti-tumour activity (PM2). This work, conducted with leading researchers at the QIMR Berghofer Medical Research Institute (Prof Michael Good & Dr Colleen Olive), established Dr Moyle’s national and international profile in the field of vaccine development, resulting in 11 peer reviewed papers, including top journals in the field (J Med Chem; J Org Chem), as well as 6 review articles and 2 invited book chapters.

Dr Moyle recently worked in the laboratory of one of the world’s premier chemical biologists, Professor Tom Muir (the Rockefeller University, NY, USA). During this time he developed an extensive knowledge of techniques for protein expression, bioconjugation, bioassays, and proteomics, which represent an essential skill set required for this proposal. As part of this work, Dr Moyle developed novel synthetic routes to generate site-specific ADP-ribose conjugated peptides and proteins. This research was hailed as a major breakthrough in the field, leading to several collaborations, and an exemplary publication in the prestigious chemistry journal JACS (PM1). This vast body of work identified the enzyme (PARP10) responsible for mono-ADP-ribosylation of histone H2B, and demonstrated interactions between this modification and several proteins, including BAL, which is associated with B cell lymphomas. In addition, a number of robust chemical methods were developed to enable the synthesis of a complete library of methyl-arginine containing histones, which were incorporated into synthetic chemically-defined chromatin to investigate the site-specific effects of arginine methylation on histone acetylation. This work led to a collaboration with colleagues at Rockefeller to investigate the effects of histone arginine methylation on transcription.

Research Interests

  • Vaccine development
    We focus on the development on methods to develop improved subunit vaccine formulations against neglected diseases, and diseases for which current vaccine formulations are not ideal. Our recent focus has been on Group A Streptococcus, which is responsible for diseases ranging from a sore throat through to heart and kidney damage, and the flesh eating disease necrotising fasciitis. We also focus on technologies that enable the site specific incorporation of potent vaccine adjuvants into protein, peptide, and carbohydrate-based antigens.
  • Protein semisynthesis/engineering
    We are interested in modern techniques for the engineering of proteins are therapeutic or diagnostic molecules, including their roles as tools to probe the functions of biochemical pathways. We use modern techniques for gene assembly (e.g. SLIC, Gibson assembly), combined with codon optimisation, optimised expression systems, and purification technologies.
  • Peptide delivery systems
    The use of peptides as drugs may suffer from issues including poor oral absorption, instability to chemical/enzymatic degradation, and an inability to reach their site of action. Using chemical techniques we can improve the stability, targeting, circulation time, potency, etc of peptide-based therapeutics.
  • siRNA, shRNA, microRNA, pDNA delivery systems
    Using peptide-based approaches to develop targeted, non-toxic systems to enhance the delivery of oligonucleotide therapeutics to their tissue, cellular and intracellular sites of action.
  • Deciphering the functional roles of post-translational modifications
    Using protein semisynthesis to produce highly pure, site-specifically post-translationally modified (e.g. ADP-ribose, methyl, phosphoryl, acetyl) proteins, which can be applied to biochemical assays to accurately determine their function, and the identity of any interacting species.

Qualifications

  • Doctor of Philosophy, The University of Queensland
  • Bachelor of Pharmacy, The University of Queensland

Publications

View all Publications

Supervision

  • Doctor Philosophy

  • Doctor Philosophy

  • Doctor Philosophy

View all Supervision

Available Projects

  • Dr Moyle is interested in taking on students with an interest in i) subunit vaccine development, ii) delivery systems for peptide therapeutics, iii) studying the function of post-translational modifications, iv) protein engineering, v) delivery systems for siRNA/shRNA/microRNA. If you are interested in working on any of these areas please contact Dr Moyle. Preference will be given to students who have their own funding (additional information on applying can be found in the biography section).

View all Available Projects

Publications

Featured Publications

Book Chapter

  • Moyle, Peter Michael (2015). Progress in vaccine development. In Richard Coico (Ed.), Current Protocols in Microbiology (pp. 18.1.1-18.1.26) Hoboken, NJ, United States: John Wiley & Sons. doi:10.1002/9780471729259.mc1801s36

  • Jackson, David C., Chua, Brendon Y., Brown, Lorena E., Moyle, Peter and Toth, Istvan (2010). Lipopeptide-based vaccines. In Myron M. Levine (Ed.), New generation vaccines 4th ed. (pp. 315-326) New York, U.S.A.; London, U.K.: Informa Healthcare.

  • Simerska, Pavla, Moyle, Peter M., Olive, Colleen and Toth, Istvan (2009). Strategies in oral immunization. In Bernkop-Schnurch, Andreas (Ed.), Oral Delivery of Macromolecular Drugs: Barriers, Strategies and Future Trends 1st ed. (pp. 195-222) New York, United States: Springer. doi:10.1007/978-1-4419-0200-9_11

Journal Article

Conference Publication

  • Simerska, Pavla, McGeary, Ross P., Abdel-Aal, Abu-Baker M., Moyle, Peter M., Olive, Colleen, Good, Michael F. and Toth, Istvan (2009). Vaccine delivery utilizing liposaccharides. In: Advances in Experimental Medicine and Biology - Peptides for Youth. The Proceedings of the 20th American Peptide Symposium. The 20th American Peptide Symposium, Montreal, Canada, (345-346). 26-30 June, 2007. doi:10.1007/978-0-387-73657-0_153

  • Toth, Istvan, Moyle, Peter M., Simerska, Pavla, Fujita, Yoshio, Olive, Colleen and Good, Michael F. (2009). Vaccine delivery: Synthesis and investigation of a highly pure, multi-epitopic lipopeptide vaccine candidate. In: Susan Del Valle, Emanuel Escher and William D. Lubell, Peptides for youth: The proceedings of the 20th American Peptide Symposium. 20th American Peptide Symposium, Montréal, Quebec, Canada, (347-349). 26-30 June 2007. doi:10.1007/978-0-387-73657-0_154

  • Fujita, Y., Moyle, P., Abdel-Aal, A., Batzloff, M., Good, M. and Toth, I. (2007). Development of a Multivalent Heteroepitopic Group A Streptococcus Vaccine. In: Wilce, J., Proceedings of the 4th International Peptide Symposium in conjunction with the 7th Australian Peptide Conference and the 2nd Asia-Pacific International Peptide Symposium, 2007. 4th International Peptide Symposium, Cairns, (1-2). 21-25 October 2007.

  • Simerska, P., Moyle, P. M., Abdel-Aal, A. M., Fujita, Y., Olive, C., Batzloff, M. R., Good, M. F. and Toth, I. (2007). Liposaccharides in Peptide Vaccine Delivery. In: Jackie Wilce, Proceedings of the 4th International Peptide Symposium in conjunction with the 7th Australian Peptide Conference and the 2nd Asia-Pacific International Peptide Symposium, 2007. 4th International Peptide Symposium, Cairns, (1-2). 21-25 October 2007.

  • Toth, I., Moyle, P. M., Simerska, P., Fujita, Y., Olive, C. and Good, M. F. (2007). Novel lipoamino acid based vaccine adjuvant carrier system. In: International Proceedings of the 13th International Congress of Immunology. International Congress of Immunology, Rio de Janeiro, Brazil, (). 21-25 August 2007.

  • Simerska, P., Abdel-Aal, A. B., McGeary, R., Moyle, P.M., Fujita, Y., Good, M.F. and Toth, I. (2007). Peptide-lipid-carbohydrate based vaccine against group A Streptococcal infection.. In: International Proceedings of the 13th International Congress of Immunology. 13th International Congress of Immunology, Rio de Janeiro, Brazil, (). August 21-25.

  • Simerska, P., Moyle, P. M., Zhong, W., McGeary, R., Abdel-Aal, A. B., Good, M. F. and Toth, I. (2007). Vaccine Delivery Utilizing Liposaccharides. In: Biopolymers: 20th American Peptide Symposium. 20th American Peptide Symposium, Montréal, Quebec, Canada, (534-534). 26-30 June 2007. doi:10.1002/bip.20783

  • Toth, I., Moyle, P. M., Simerska, P., Fujita, Y., Olive, C. and Good, M. F. (2007). Vaccine delivery: Synthesis and investigation of a highly pure, multi-epitopic lipopeptide vaccine candidate. In: Biopolymers. 20th American Peptide Symposium, Montréal, Quebec, Canada, (535-535). 26-30 June 2007. doi:10.1002/bip.20783

  • Moyle, Peter M., Horvath, Aniko, Karpati, Levente, Olive, Colleen, Barozzi, Nadia, Wimmer, Norbert, Good, Michael and Toth, Istvan (2006). Development of peptide vaccines against HPV-16 associated cervical cancer and group a streptococci. In: Understanding Biology Using Peptides. 19th American Peptide Symposium, San Diego, United States, (407-408). 18-23 June 2005. doi:10.1007/978-0-387-26575-9_169

  • Simerska, P., Moyle, P. M., Olive, C., Sun, H. K., Ho, M. F., Pandy, M. G., Dyer, J. R., Burgess, M. L. and Toth, I. (2006). Development of vaccines for the prevention and/or treatment of human papillomavirus type-16 associated cervical cancer. In: Proceedings of GPEN 2006. GPEN 2006, Lawrence, USA, (Abstract). 25-27 October, 2006.

  • Simerska, P, Moyle, P. M., Olive, C., Sun, H., Ho, M.-F., Pandy, M.G., Dyer, J. R., Burgess, M L and Toth, I (2006). Development of vaccines for the prevention and/or treatment of human papillomavirus type-16 associated cervical cancer. In: Abstracts of the Brisbane Biological & Organic Chemistry Symposium. Brisbane Biological & Organic Chemistry Symposium, Brisbane, (Abstract). 24 November 2006.

  • Moyle, P. M., Olive, C., Ho, M.-F., Good, M F and Toth, I (2006). Highly pure, multi-epitopic lipopeptide vaccine delivery system: Synthesis and investigation. In: Brisbane Biological & Organic Chemistry Symposium. Brisbane Biological & Organic Chemistry Symposium, Brisbane, (Abstract). 24 Nov 2006.

  • Moyle, P. M., Olive, C., Ho, M.-F. and Toth, I (2006). Highly pure, multi-epitopic lipopeptide vaccine delivery system: Synthesis and investigation. In: GPEN 2006. GPEN 2006, Lawrence, USA, (Abstract). 25 - 27 Oct, 2006.

  • Simerska, P., Bong, Y. K., McGeary, R. P., Moyle, P. M. and Toth, I. (2006). Liposaccharide based vaccine carrier adjuvant systems. In: Drug Design Amongst the Vines, Hunter Valley, (). 3 - 7 December 2006.

  • Toth, I., Moyle, P. M., Simerska, P., Fujita, Y., Olive, C. and Good, M. F. (2006). Liposaccharides in peptide, gene and vaccine delivery. In: Proceedings of Drug Design Amongst the Vines. Drug Design Amongst the Vines, Hunter Valley, (Abstract). 3 - 7 December, 2006.

  • Toth, I, Moyle, P. M., Koda, Y., Olive, C. and Good, M F (2006). Liposaccharides in peptide, gene and vaccine delivery. In: 10th Naples Workshop on Bioactive Peptides. 10th Naples Workshop on Bioactive Peptides, Naples, (Abstract). 11-14 June, 2006.

  • Toth, I., Moyle, P. M., Koda, Y., Olive, C. and Good, M. F. (2006). Liposaccharides in peptide, gene and vaccine delivery. In: 33rd Annual Meeting & Exposition of the Controlled Release Society. 33rd Annual Meeting and Exposition of the Controlled Release Soy, Vienna, (Abstract). 22-26 July, 2006.

  • Fujita, Y, Moyle, P. M., Olive, C., Good, M F and Toth, I (2006). Multi-epitopic lipid core peptide vaccine with broad protective immune responses against Group A streptococcus. In: 29th European Peptide Symposium. 29th European Peptide Symposium, Gdansk, (Abstract). 3-8 Sept, 2006.

  • Moyle, Peter Michael, Olive, Colleen, Karpati, Levente, Barozzi, Nadia, Ho, Mei-Fong, Dyer, Joanne, Sun, Hsien Kuo, Good, Michael and Toth, Istvan (2006). Synthesis and immunological evaluation of M protein targeted tetra-valent and tri-valent group A streptococcal vaccine candidates based on the lipid-core peptide system. In: International Journal of Peptide Research and Therapeutics: Proceedings of the 6th Australian Peptide Conference. 6th Australian Peptide Conference, Whitsunday Islands, Australia, (317-326). 9-14 October, 2005. doi:10.1007/s10989-006-9021-8

  • Toth, I., Moyle, P. M., Karpati, L., Horvath, A., Olive, C. and Good, M. F. (2006). The lipid core peptide system in vaccine delivery. In: International Congress Series: Streptococci - New Insights Into an Old Enemy - Proceedings of the XVIth Lancefield International Symposium on Streptococci and Streptococcal Diseases, held in Palm Cove, Australia between 25 and 29 September, 2005. XVIth Lancefield International Symposium on Streptococci and Streptococcal Diseases, Palm Cove, Australia, (307-310). 25 and 29 September 2005. doi:10.1016/j.ics.2005.11.057

  • Moyle, Peter M., Olive, Colleen, Sun, Hsien, Good, Michael and Toth, Istvan (2005). The synthesis and immunological activity of a lipid-core peptide (LCP) based Human Papillomavirus (HPV) type-16 vaccine. In: Peptides 2004: Proceedings of the Third International and Twenty-Eighth European Peptide Symposium. 3rd International Peptide Symposium and 28th European Peptide Symposium, Prague, Czech Republic, (1139-1140). 5-10 September 2004.

  • Toth, I., Horvath, A., Karpati, L., Moyle, P., Olive, C. and Good, M. (2004). Liposaccharides for drug and vaccine delivery. In: 3rd International and 28th European Peptide Symposium, Prague, Czech Republic, (294-294). 5–10 September 2004. doi:10.1002/psc.618

  • Moyle, P.M., Mcgeary, R. P. and Toth, I. (2001). Solid-phase synthesis of oligosaccharides utilising a novel Dde-based linker. In: S.A. Charman, Proceedings of APSA Annual Conference 2001. APSA Annual Conference 2001, Melbourne, (105-105). 9-12 Dec 2001.

Other Outputs

PhD and MPhil Supervision

Current Supervision

  • Doctor Philosophy — Principal Advisor

  • Doctor Philosophy — Principal Advisor

    Other advisors:

  • Doctor Philosophy — Joint Principal Advisor

    Other advisors:

  • Doctor Philosophy — Joint Principal Advisor

    Other advisors:

  • Doctor Philosophy — Associate Advisor

    Other advisors:

  • Doctor Philosophy — Associate Advisor

    Other advisors:

  • Doctor Philosophy — Associate Advisor

    Other advisors:

Possible Research Projects

Note for students: The possible research projects listed on this page may not be comprehensive or up to date. Always feel free to contact the staff for more information, and also with your own research ideas.

  • Dr Moyle is interested in taking on students with an interest in i) subunit vaccine development, ii) delivery systems for peptide therapeutics, iii) studying the function of post-translational modifications, iv) protein engineering, v) delivery systems for siRNA/shRNA/microRNA. If you are interested in working on any of these areas please contact Dr Moyle. Preference will be given to students who have their own funding (additional information on applying can be found in the biography section).