The University of Queensland

The dynamin GTPase mediates regenerative axonal fusion in <i>Caenorhabditis elegans</i> by regulating fusogen levels

Vijayaraghavan, Tarika, Dhananjay, Samiksha, Ho, Xue Yan, Giordano-Santini, Rosina, Hilliard, Massimo and Neumann, Brent (2023). The dynamin GTPase mediates regenerative axonal fusion in Caenorhabditis elegans by regulating fusogen levels. PNAS Nexus, 2 (5). doi: 10.1093/pnasnexus/pgad114

Impaired signaling for neuromuscular synaptic maintenance is a feature of Motor Neuron Disease

Ding, Qiao, Kesavan, Kaamini, Lee, Kah Meng, Wimberger, Elyse, Robertson, Thomas, Gill, Melinder, Power, Dominique, Chang, Jeryn, Fard, Atefeh T., Mar, Jessica C., Henderson, Robert D., Heggie, Susan, McCombe, Pamela A., Jeffree, Rosalind L., Colditz, Michael J., Hilliard, Massimo A., Ng, Dominic C. H., Steyn, Frederik J., Phillips, William D., Wolvetang, Ernst J., Ngo, Shyuan T. and Noakes, Peter G. (2022). Impaired signaling for neuromuscular synaptic maintenance is a feature of Motor Neuron Disease. Acta Neuropathologica Communications, 10 (1) 61, 61. doi: 10.1186/s40478-022-01360-5

The metalloprotease ADM-4/ADAM17 promotes axonal repair

Ho, Xue Yan, Coakley, Sean, Amor, Rumelo, Anggono, Victor and Hilliard, Massimo A. (2022). The metalloprotease ADM-4/ADAM17 promotes axonal repair. Science Advances, 8 (11) eabm2882, eabm2882. doi: 10.1126/sciadv.abm2882

Neuron-epidermal attachment protects hyper-fragile axons from mechanical strain

Bonacossa-Pereira, Igor, Coakley, Sean and Hilliard, Massimo A. (2022). Neuron-epidermal attachment protects hyper-fragile axons from mechanical strain. Cell Reports, 38 (10) 110501, 110501. doi: 10.1016/j.celrep.2022.110501

TDP-43 mutation affects stress granule dynamics in differentiated NSC-34 motoneuron-like cells

Ding, Qiao, Chaplin, Justin, Morris, Matthew J., Hilliard, Massimo A., Wolvetang, Ernst, Ng, Dominic C. H. and Noakes, Peter G. (2021). TDP-43 mutation affects stress granule dynamics in differentiated NSC-34 motoneuron-like cells. Frontiers in Cell and Developmental Biology, 9 611601, 611601. doi: 10.3389/fcell.2021.611601

Modular transient nanoclustering of activated β2-adrenergic receptors revealed by single-molecule tracking of conformation-specific nanobodies

Gormal, Rachel S., Padmanabhan, Pranesh, Kasula, Ravikiran, Bademosi, Adekunle T., Coakley, Sean, Giacomotto, Jean, Blum, Ailisa, Joensuu, Merja, Wallis, Tristan P., Lo, Harriet P., Budnar, Srikanth, Rae, James, Ferguson, Charles, Bastiani, Michele, Thomas, Walter G., Pardon, Els, Steyaert, Jan, Yap, Alpha S., Goodhill, Geoffrey J., Hilliard, Massimo A., Parton, Robert G. and Meunier, Frédéric A. (2020). Modular transient nanoclustering of activated β2-adrenergic receptors revealed by single-molecule tracking of conformation-specific nanobodies. Proceedings of the National Academy of Sciences of the United States of America, 117 (48), 30476-30487. doi: 10.1073/pnas.2007443117

Fusogen-mediated neuron−neuron fusion disrupts neural circuit connectivity and alters animal behavior

Giordano-Santini, Rosina, Kaulich, Eva, Galbraith, Kate M., Ritchie, Fiona K., Wang, Wei, Li, Zhaoyu and Hilliard, Massimo A. (2020). Fusogen-mediated neuron−neuron fusion disrupts neural circuit connectivity and alters animal behavior. Proceedings of the National Academy of Sciences, 117 (37), 201919063-23065. doi: 10.1073/pnas.1919063117

Epidermal control of axonal attachment via β-spectrin and the GTPase-activating protein TBC-10 prevents axonal degeneration

Coakley, Sean, Ritchie, Fiona K., Galbraith, Kate M. and Hilliard, Massimo A. (2020). Epidermal control of axonal attachment via β-spectrin and the GTPase-activating protein TBC-10 prevents axonal degeneration. Nature Communications, 11 (1) 133, 1-12. doi: 10.1038/s41467-019-13795-x

Axonal repair by fusion: pitfalls, consequences and solutions

Neumann, Brent and Hilliard, Massimo A. (2019). Axonal repair by fusion: pitfalls, consequences and solutions. FASEB Journal, 33 (12), 13071-13074. doi: 10.1096/fj.201901407R

Disruption of RAB-5 increases EFF-1 fusogen availability at the cell surface and promotes the regenerative axonal fusion capacity of the neuron

Linton, Casey, Riyadh, M. Asrafuzzaman, Ho, Xue Yan, Neumann, Brent, Giordano-Santini, Rosina and Hilliard, Massimo A. (2019). Disruption of RAB-5 increases EFF-1 fusogen availability at the cell surface and promotes the regenerative axonal fusion capacity of the neuron. Journal of Neuroscience, 39 (15), 2823-2836. doi: 10.1523/jneurosci.1952-18.2019

Axonal fusion: an alternative and efficient mechanism of nerve repair

Neumann, Brent, Linton, Casey, Giordano-Santini, Rosina and Hilliard, Massimo A. (2019). Axonal fusion: an alternative and efficient mechanism of nerve repair. Progress in Neurobiology, 173, 88-101. doi: 10.1016/j.pneurobio.2018.11.004

Neuronal sub-compartmentalization: a strategy to optimize neuronal function

Donato, Alessandra, Kagias, Konstantinos, Zhang, Yun and Hilliard, Massimo A. (2019). Neuronal sub-compartmentalization: a strategy to optimize neuronal function. Biological Reviews. doi: 10.1111/brv.12487

6-OHDA-induced dopaminergic neurodegeneration in Caenorhabditis elegans is promoted by the engulfment pathway and inhibited by the transthyretin-related protein TTR-33

Offenburger, Sarah-Lena, Ho, Xue Yan, Tachie-Menson, Theresa, Coakley, Sean, Hilliard, Massimo A. and Gartner, Anton (2018). 6-OHDA-induced dopaminergic neurodegeneration in Caenorhabditis elegans is promoted by the engulfment pathway and inhibited by the transthyretin-related protein TTR-33. PLoS Genetics, 14 (1) e1007125, 1-27. doi: 10.1371/journal.pgen.1007125

Defects in synaptic transmission at the neuromuscular junction precedes motor deficits in a TDP-43Q331K transgenic mouse model of amyotrophic lateral sclerosis

Chand, Kirat K., Lee, Kah Meng, Lee, John D., Qiu, Hao, Willis, Emily F., Lavidis, Nickolas A., Hilliard, Massimo A. and Noakes, Peter G. (2018). Defects in synaptic transmission at the neuromuscular junction precedes motor deficits in a TDP-43Q331K transgenic mouse model of amyotrophic lateral sclerosis. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 32 (5), fj201700835R-2689. doi: 10.1096/fj.201700835R

The heterochronic gene lin-14 controls axonal degeneration in C. elegans neurons

Ritchie, Fiona K., Knable, Rhianna, Chaplin, Justin, Gursanscky, Rhiannon, Gallegos, Maria, Neumann, Brent and Hilliard, Massimo A. (2017). The heterochronic gene lin-14 controls axonal degeneration in C. elegans neurons. Cell Reports, 20 (12), 2955-2965. doi: 10.1016/j.celrep.2017.08.083

A multi-trap microfluidic chip enabling longitudinal studies of nerve regeneration in Caenorhabditis elegans

Gokce, Sertan Kutal, Hegarty, Evan Marley, Mondal, Sudip, Zhao, Peisen, Ghorashian, Navid, Hilliard, Massimo A. and Ben-Yakar, Adela (2017). A multi-trap microfluidic chip enabling longitudinal studies of nerve regeneration in Caenorhabditis elegans. Scientific Reports, 7 (9837) 9837, 9837. doi: 10.1038/s41598-017-10302-4

Phosphatidylserine save-me signals drive functional recovery of severed axons in Caenorhabditis elegans

Abay, Zehra C, Wong, Michelle Yu-Ying, Teoh, Jean-Sébastien, Vijayaraghavan, Tarika, Hilliard, Massimo A and Neumann, Brent (2017). Phosphatidylserine save-me signals drive functional recovery of severed axons in Caenorhabditis elegans. Proceedings of the National Academy of Sciences of the United States of America, 114 (47), 1-10. doi: 10.1073/pnas.1703807114

Cortical synaptic and dendritic spine abnormalities in a presymptomatic TDP-43 model of amyotrophic lateral sclerosis

Fogarty, Matthew J., Klenowski, Paul M., Lee, John D., Drieberg-Thompson, Joy R., Bartlett, Selena E., Ngo, Shyuan T., Hilliard, Massimo A., Bellingham, Mark C. and Noakes, Peter G. (2016). Cortical synaptic and dendritic spine abnormalities in a presymptomatic TDP-43 model of amyotrophic lateral sclerosis. Scientific Reports, 6 (1) 37968, 37968. doi: 10.1038/srep37968

Cell-cell fusion in the nervous system: alternative mechanisms of development, injury and repair

Giordano-Santini, Rosina, Linton, Casey and Hilliard, Massimo A. (2016). Cell-cell fusion in the nervous system: alternative mechanisms of development, injury and repair. Seminars in Cell and Developmental Biology, 60, 146-154. doi: 10.1016/j.semcdb.2016.06.019

Neuron-specific knock-down of SMN1 causes neuron degeneration and death through an apoptotic mechanism

Gallotta, Ivan, Mazzarella, Nadia, Donato, Alessandra, Esposito, Alessandro, Chaplin, Justin C., Castro, Silvana, Zampi, Giuseppina, Battaglia, Giorgio S., Hilliard, Massimo A., Bazzicalupo, Paolo and Di Schiavi, Elia (2016). Neuron-specific knock-down of SMN1 causes neuron degeneration and death through an apoptotic mechanism. Human Molecular Genetics, 25 (12), 2564-2577. doi: 10.1093/hmg/ddw119

The apoptotic engulfment machinery regulates axonal degeneration in C. elegans neurons

Nichols, Annika L.A., Meelkop, Ellen, Linton, Casey, Giordano-Santini, Rosina, Sullivan, Robert K., Donato, Alessandra, Nolan, Cara, Hall, David H., Xue, Ding, Neumann, Brent and Hilliard, Massimo A. (2016). The apoptotic engulfment machinery regulates axonal degeneration in C. elegans neurons. Cell Reports, 14 (7), 1673-1683. doi: 10.1016/j.celrep.2016.01.050

EFF-1-mediated regenerative axonal fusion requires components of the apoptotic pathway

Neumann, Brent, Coakley, Sean, Giordano-Santini, Rosina, Linton, Casey, Lee, Eui Seung, Nakagawa, Akihisa, Xue, Ding and Hilliard, Massimo A. (2015). EFF-1-mediated regenerative axonal fusion requires components of the apoptotic pathway. Nature, 517 (7533), 219-222. doi: 10.1038/nature14102

A multi-channel device for high-density target-selective stimulation and long-term monitoring of cells and subcellular features in C. elegans

Lee, Hyewon, Kim, Shin Ae, Coakley, Sean, Mugno, Paula, Hammarlund, Marc, Hilliard, Massimo A. and Lu, Hang (2014). A multi-channel device for high-density target-selective stimulation and long-term monitoring of cells and subcellular features in C. elegans. Lab on a Chip - Miniaturisation for Chemistry and Biology, 14 (23), 4513-4522. doi: 10.1039/c4lc00789a

Loss of MEC-17 leads to microtubule instability and axonal degeneration

Neumann, Brent and Hilliard, Massimo A. (2014). Loss of MEC-17 leads to microtubule instability and axonal degeneration. Cell Reports, 6 (1), 93-103. doi: 10.1016/j.celrep.2013.12.004

Rapid and permanent neuronal inactivation in vivo via subcellular generation of reactive oxygen with the use of KillerRed

Williams, Daniel C., El Bejjani, Rachid, Mugno Ramirez, Paula, Coakley, Sean, Kim, Shin Ae, Lee, Hyewon, Wen, Quan, Samuel, Aravi, Lu, Hang, Hilliard, Massimo A. and Hammarlund, Marc (2013). Rapid and permanent neuronal inactivation in vivo via subcellular generation of reactive oxygen with the use of KillerRed. Cell Reports, 5 (2), 553-563. doi: 10.1016/j.celrep.2013.09.023

A dominant mutation in mec-7/β-tubulin affects axon development and regeneration in Caenorhabditis elegans neurons

Kirszenblat, Leonie, Neumann, Brent, Coakley, Sean and Massimo Hilliard (2013). A dominant mutation in mec-7/β-tubulin affects axon development and regeneration in Caenorhabditis elegans neurons. Molecular Biology of the Cell, 24 (3), 285-296. doi: 10.1091/mbc.E12-06-0441

A core metabolic enzyme mediates resistance to phosphine gas

Schlipalius, David I., Valmas, Nicholas, Tuck, Andrew G., Jagadeesan, Rajeswaran, Ma, Li, Kaur, Ramandeep, Goldinger, Anita, Anderson, Cameron, Kuang, Jujiao, Zuryn, Steven, Mau, Yosep S., Cheng, Qiang, Collins, Patrick J., Nayak, Manoj K., Schirra, Horst Joachim, Hilliard, Massimo A. and Ebert, Paul R. (2012). A core metabolic enzyme mediates resistance to phosphine gas. Science, 338 (6108), 807-810. doi: 10.1126/science.1224951

Laterally orienting C. elegans using geometry at microscale for high-throughput visual screens in neurodegeneration and neuronal development studies

Cáceres, Ivan de Carlos, Valmas, Nicholas, Hilliard, Massimo A. and Lu, Hang (2012). Laterally orienting C. elegans using geometry at microscale for high-throughput visual screens in neurodegeneration and neuronal development studies. PLoS One, 7 (4) e35037, e35037.1-e35037.8. doi: 10.1371/journal.pone.0035037

LIN-44/Wnt directs dendrite outgrowth through LIN-17/Frizzled in C. elegans neurons

Kirszenblat, Leonie, Pattabiraman, Divya and Hilliard, Massimo A. (2011). LIN-44/Wnt directs dendrite outgrowth through LIN-17/Frizzled in C. elegans neurons. PLoS Biology, 9 (9) e1001157, 0nline. doi: 10.1371/journal.pbio.1001157

Axonal regeneration proceeds through specific axonal fusion in transected C. elegans neurons

Neumann, Brent, Nguyen, Ken C. Q., Hall, David H., Ben-Yakar, Adela and Hilliard, Massimo A. (2011). Axonal regeneration proceeds through specific axonal fusion in transected C. elegans neurons. Developmental Dynamics, 240 (6), 1365-1372. doi: 10.1002/dvdy.22606

Big ideas for small brains: What can psychiatry learn from worms, flies, bees and fish?

Burne, T. H. J., Scott, E., van Swinderen, B., Hilliard, M., Reinhard, J., Claudianos, C., Eyles, D. W. and McGrath, J. J. (2011). Big ideas for small brains: What can psychiatry learn from worms, flies, bees and fish?. Molecular Psychiatry, 16 (1), 7-16. doi: 10.1038/mp.2010.35

Axonal degeneration and regeneration: a mechanistic tug-of-war

Hilliard, Massimo (2009). Axonal degeneration and regeneration: a mechanistic tug-of-war. Journal Of Neurochemistry, 108 (1), 23-32. doi: 10.1111/j.1471-4159.2008.05754.x

Femtosecond laser nanoaxotomy lab-on-a-chip for in vivo nerve regeneration studies

Guo, Samuel X., Bourgeois, Frederic, Chokshi, Trushal, Durr, Nicholas J., Hilliard, Massimo A., Chronis, Nikos and Ben-Yakar, Adela (2008). Femtosecond laser nanoaxotomy lab-on-a-chip for in vivo nerve regeneration studies. Nature Methods, 5 (6), 531-533. doi: 10.1038/nmeth.1203

Global 'worming'

Chalasani, Sreekanth H., Feinberg, Evan H. and Hilliard, Massimo A. (2007). Global 'worming'. Genome Biology, 8 (9) 314, 314-1-314-3. doi: 10.1186/gb-2007-8-9-314

Multiple Wnts and frizzled receptors regulate anteriorly directed cell and growth cone migrations in Caenorhabditis elegans

Pan, Chun-Liang, Howell, James Endres, Clark, Scott G., Hilliard, Massimo, Cordes, Shaun, Bargmann, Cornelia I. and Garriga, Gian (2006). Multiple Wnts and frizzled receptors regulate anteriorly directed cell and growth cone migrations in Caenorhabditis elegans. Developmental cell, 10 (3), 367-377. doi: 10.1016/j.devcel.2006.02.010

Wnt signals and Frizzled activity orient anterior-posterior axon outgrowth in C. elegans

Hilliard, Massimo A. and Bargmann, Cornelia I. (2006). Wnt signals and Frizzled activity orient anterior-posterior axon outgrowth in C. elegans. Developmental Cell, 10 (3), 379-390. doi: 10.1016/j.devcel.2006.01.013

The Zona Pellucida domain containing proteins, CUT-1, CUT-3 and CUT-5, play essential roles in the development of the larval alae in Caenorhabditis elegans

Sapio, Maria Rosaria, Hilliard, Massimo A., Cermola, Michele, Favre, Reneé and Bazzicalupo, Paolo (2005). The Zona Pellucida domain containing proteins, CUT-1, CUT-3 and CUT-5, play essential roles in the development of the larval alae in Caenorhabditis elegans. Developmental Biology, 282 (1), 231-245. doi: 10.1016/j.ydbio.2005.03.011

In vivo imaging of C. elegans ASH neurons: Cellular response and adaptation to chemical repellents

Hilliard, Massimo A., Apicella, Alfonso J., Kerr, Rex, Suzuki, Hiroshi, Bazzicalupo, Paolo and Schafer, William R. (2005). In vivo imaging of C. elegans ASH neurons: Cellular response and adaptation to chemical repellents. The EMBO Journal 24, 1489-1489, 24 (1), 63-72. doi: 10.1038/sj.emboj.7600493

Worms taste bitter: ASH neurons, QUI-1, GPA-3 and ODR-3 mediate quinine avoidance in Caenorhabditis elegans

Hilliard, Massimo A., Bergamasco, Carmela, Arbucci, Salvatore, Plasterk, Ronald H. A. and Bazzicalupo, Paolo (2004). Worms taste bitter: ASH neurons, QUI-1, GPA-3 and ODR-3 mediate quinine avoidance in Caenorhabditis elegans. The EMBO Journal, 23 (5), 1101-1111. doi: 10.1038/sj.emboj.7600107

The Kallmann syndrome gene homolog in C. elegans is involved in epidermal morphogenesis and neurite branching

Rugarli, Elena I., Di Schiavi, Elia, Hilliard, Massimo A., Arbucci, Salvatore, Ghezzi, Cristina, Facciolli, Anna, Coppola, Giuseppe, Ballabio, Andrea and Bazzicalupo, Paolo (2002). The Kallmann syndrome gene homolog in C. elegans is involved in epidermal morphogenesis and neurite branching. Development, 129 (5), 1283-1294.

C. elegans responds to chemical repellents by integrating sensory inputs from the head and the tail

Hilliard, Massimo A., Bargmann, Cornelia I. and Bazzicalupo, Paolo (2002). C. elegans responds to chemical repellents by integrating sensory inputs from the head and the tail. Current Biology, 12 (9), 730-734. doi: 10.1016/S0960-9822(02)00813-8

Roles for Caenorhabditis elegans rad-51 in meiosis and in resistance to ionizing radiation during development

Rinaldo, Cinzia, Bazzicalupo, Paolo, Ederle, Sara, Hilliard, Massimo and La Volpe, Adriana (2002). Roles for Caenorhabditis elegans rad-51 in meiosis and in resistance to ionizing radiation during development. Genetics, 160 (2), 471-479.

The Eps15 C. elegans homologue EHS-1 is implicated in synaptic vesicle recycling

Salcini, Anna Elisabetta, Hilliard, Massimo Antonio, Croce, Assunta, Arbucci, Salvatore, Luzzi, Paola, Tacchetti, Carlo, Daniell, Laurie, De Camilli, Pietro, Pelicci, Pier Giuseppe, Di Fiore, Pier Paolo and Bazzicalupo, Paolo (2001). The Eps15 C. elegans homologue EHS-1 is implicated in synaptic vesicle recycling. Nature Cell Biology, 3 (8), 755-760. doi: 10.1038/35087075

Neurons and genes involved in chemical sensitivity in nematodes

Bazzicalupo, P., Hilliard, M., Lewis, E., De Riso, L., Sebastiano, M. and Ristoratore, F. (1994). Neurons and genes involved in chemical sensitivity in nematodes. Parasite, 1 (1), 58-60. doi: 10.1051/parasite/199401s1058

The metalloprotease ADM-4 promotes regenerative axonal fusion

Ho, X.Y., Coakley, S. and Hilliard, M.A. (2019). The metalloprotease ADM-4 promotes regenerative axonal fusion. International Worm Meeting, UCLA, September. Rockville, MD, United States: Genetics Society of America.

High-throughput multichannel array device for selective stimulation and long-term in vivo imaging in C. elegans

Kim, Shin Ae, Lee, Hyewon, Hilliard, Massimo and Lu, Hang (2014). High-throughput multichannel array device for selective stimulation and long-term in vivo imaging in C. elegans. Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting, Atlanta GA, United States, 16-21 November 2014. American Institute of Chemical Engineers.

Fast target-selective chemical & optical stimulation based on high-throughput multi-channel imaging device

Lee, Hyewon, Kim, Shin Ae, Aubry, Guillaume, Mugno, Paula, Hilliard, Massimo and Lu, Hang (2012). Fast target-selective chemical & optical stimulation based on high-throughput multi-channel imaging device. 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 28 October - 1 November 2012. San Diego, CA, United States: Chemical and Biological Microsystems Society.

Femtosecond laser nanosurgery in microfluidic devices and its emerging role in nerve regeneration studies

Guo, Samuel X., Bourgeois, Frederic, Chokshi, Trushal, Durr, Nicholas J., Hilliard, Massimo, Chronis, Nikos and Ben-Yakar, Adela (2008). Femtosecond laser nanosurgery in microfluidic devices and its emerging role in nerve regeneration studies. doi: 10.1109/LEOS.2008.4688576