The University of Queensland

A brief introduction to neural flow cytometry from a practical perspective

Osborne, Geoffrey W. (2015). A brief introduction to neural flow cytometry from a practical perspective. Neural surface antigens: from basic biology towards biomedical applications. (pp. 15-26) edited by Jan Pruszak. London, United Kingdom: Elsevier. doi: 10.1016/B978-0-12-800781-5.00002-5

Flow cytometry of neural cells

Osborne, Geoffrey W. (2013). Flow cytometry of neural cells. Neural Progenitor Cells: Methods and Protocols. (pp. 135-144) edited by Brent A. Reynolds and Loic P. Deleyrolle. New York, NY, United States: Humana Press. doi: 10.1007/978-1-62703-574-3_12

Recent advances in flow cytometric cell sorting

Osborne, Geoffrey W. (2011). Recent advances in flow cytometric cell sorting. Recent advances in cytometry. Part A: Instrumentation, methods. (pp. 533-556) edited by Zbigniew Darzynkiewicz, Elena Holden, Alberto Orfao, William Telford and Donald Wlodkowic. Maryland Heights, MO, United States: Academic Press. doi: 10.1016/b978-0-12-374912-3.00021-3

Practical considerations for flow cytometric sorting of stem cells

Osborne, Geoffrey W. (2010). Practical considerations for flow cytometric sorting of stem cells. Application of flow cytometry in stem cell research and tissue regeneration. (pp. 13-23) edited by Awtar Krishan, H. Krishnamurthy and Satish Totey. Hoboken, New Jersey: John Wiley & Sons, Inc.. doi: 10.1002/9780470631119.ch2

Complement receptor C3aR1 controls neutrophil mobilization following spinal cord injury through physiological antagonism of CXCR2

Brennan, Faith H., Jogia, Trisha, Gillespie, Ellen R., Blomster, Linda V., Li, Xaria X., Nowlan, Bianca, Williams, Gail M., Jacobson, Esther, Osborne, Geoff W., Meunier, Frederic A., Taylor, Stephen M., Campbell, Kate E., MacDonald, Kelli P.A., Levesque, Jean-Pierre, Woodruff, Trent M. and Ruitenberg, Marc J. (2019). Complement receptor C3aR1 controls neutrophil mobilization following spinal cord injury through physiological antagonism of CXCR2. JCI Insight, 4 (9) e98254. doi: 10.1172/jci.insight.98254

Identification of CD24 as a marker of Patched1 deleted medulloblastoma-initiating neural progenitor cells

Robson, Jonathan P., Remke, Marc, Kool, Marcel, Julian, Elaine, Korshunov, Andrey, Pfister, Stefan M., Osborne, Geoffrey W., Taylor, Michael D., Wainwright, Brandon and Reynolds, Brent A. (2019). Identification of CD24 as a marker of Patched1 deleted medulloblastoma-initiating neural progenitor cells. PLoS ONE, 14 (1) e0210665, e0210665. doi: 10.1371/journal.pone.0210665

Functional domain analysis of SOX18 transcription factor using a single-chain variable fragment-based approach

Fontaine, Frank R., Goodall, Stephen, Prokop, Jeremy W., Howard, Christopher B., Moustaqil, Mehdi, Kumble, Sumukh, Rasicci, Daniel T., Osborne, Geoffrey W., Gambin, Yann, Sierecki, Emma, Jones, Martina L., Zuegg, Johannes, Mahler, Stephen and Francois, Mathias (2018). Functional domain analysis of SOX18 transcription factor using a single-chain variable fragment-based approach. mAbs, 10 (4), 1-11. doi: 10.1080/19420862.2018.1451288

Developmental vitamin D (DVD) deficiency reduces Nurr1 and TH expression in post-mitotic dopamine neurons in rat mesencephalon

Luan, Wei, Hammond, Luke Alexander, Cotter, Edmund, Osborne, Geoffrey William, Alexander, Suzanne Adele, Nink, Virginia, Cui, Xiaoying and Eyles, Darryl Walter (2017). Developmental vitamin D (DVD) deficiency reduces Nurr1 and TH expression in post-mitotic dopamine neurons in rat mesencephalon. Molecular Neurobiology, 55 (3), 2443-2453. doi: 10.1007/s12035-017-0497-3

Targeting membrane proteins for antibody discovery using phage display

Jones, Martina L., Alfaleh, Mohamed A., Kumble, Sumukh, Zhang, Shuo, Osborne, Geoffrey W., Yeh, Michael, Arora, Neetika, Hou, Jeff Jia Cheng, Howard, Christopher B., Chin, David Y. and Mahler, Stephen M. (2016). Targeting membrane proteins for antibody discovery using phage display. Scientific Reports, 6 (1) 26240, 26240. doi: 10.1038/srep26240

Purification of neural precursor cells reveals the presence of distinct, stimulus-specific subpopulations of quiescent precursors in the adult mouse hippocampus

Jhaveri, Dhanisha J., O'Keeffe, Imogen, Robinson, Gregory J., Zhao, Qiong-Yi, Zhang, Zong Hong, Nink, Virginia, Narayanan, Ramesh K., Osborne, Geoffrey W., Wray, Naomi R. and Bartlett, Perry F. (2015). Purification of neural precursor cells reveals the presence of distinct, stimulus-specific subpopulations of quiescent precursors in the adult mouse hippocampus. Journal of Neuroscience, 35 (21), 8132-8144. doi: 10.1523/JNEUROSCI.0504-15.2015

A novel flow cytometric method to assess inflammasome formation

Sester, David P., Thygesen, Sara J., Sagulenko, Vitaliya, Vajjhala, Parimala R., Cridland, Jasmyn A., Vitak, Nazarii, Chen, Kaiwen W., Osborne, Geoffrey W., Schroder, Kate and Katryn J. Stacey (2015). A novel flow cytometric method to assess inflammasome formation. The Journal of Immunology, 194 (1), 455-462. doi: 10.4049/jimmunol.1401110

Development of a novel cell sorting method that samples population diversity in flow cytometry

Osborne, Geoffrey W., Andersen, Stacey B. and Battye, Francis L. (2015). Development of a novel cell sorting method that samples population diversity in flow cytometry. Cytometry Part A, 87 (11), 1047-1051. doi: 10.1002/cyto.a.22678

Absolute counting of neutrophils in whole blood using flow cytometry

Brunck, Marion E. G., Andersen, Stacey B., Timmins, Nicholas E., Osborne, Geoffrey W. and Nielsen, Lars K. (2014). Absolute counting of neutrophils in whole blood using flow cytometry. Cytometry Part A, 85 (12), 1057-1064. doi: 10.1002/cyto.a.22503

Effect of a hemiuroid trematode on the hemocyte immune parameters of the cockle Anadara trapezia

Dang, Cécile, Cribb, Thomas H., Osborne, Geoffrey, Kawasaki, Minami, Bedin, Anne-Sophie and Barnes, Andrew C. (2013). Effect of a hemiuroid trematode on the hemocyte immune parameters of the cockle Anadara trapezia. Fish and Shellfish Immunology, 35 (3), 951-956. doi: 10.1016/j.fsi.2013.07.010

A novel fluorescent reporter CDy1 enriches for neural stem cells derived from the murine brain

Vukovic, Jana, Bedin, Anne-Sophie, Bartlett, Perry F. and Osborne, Geoffrey W. (2013). A novel fluorescent reporter CDy1 enriches for neural stem cells derived from the murine brain. Stem Cells and Development, 22 (16), 2341-2345. doi: 10.1089/scd.2012.0660

Glioma surgical aspirate: a viable source of tumor tissue for experimental research

Day, Bryan W., Stringer, Brett W., Wilson, John, Jeffree, Rosalind L., Jamieson, Paul R., Ensbey, Kathleen S., Bruce, Zara C., Inglis, Po, Allan, Suzanne, Winter, Craig, Tollesson, Gert, Campbell, Scott, Lucas, Peter, Findlay, Wendy, Kadrian, David, Johnson, David, Robertson, Thomas, Johns, Terrance G., Bartlett, Perry F., Osborne, Geoffrey W. and Boyd, Andrew W. (2013). Glioma surgical aspirate: a viable source of tumor tissue for experimental research. Cancers, 5 (2), 357-371. doi: 10.3390/cancers5020357

EphA3 Maintains Tumorigenicity and Is a Therapeutic Target in Glioblastoma Multiforme

Day, Bryan W., Stringer, Brett W., Al-Ejeh, Fares, Ting, Michael J., Wilson, John, Ensbey, Kathleen S., Jamieson, Paul R., Bruce, Zara C., Lim, Yi Chieh, Offenhauser, Carolin, Charmsaz, Sara, Cooper, Leanne T., Ellacott, Jennifer K., Harding, Angus, Leveque, Lucie, Inglis, Po, Allan, Suzanne, Walker, David G., Lackmann, Martin, Osborne, Geoffrey, Khanna, Kum Kum, Reynolds, Brent A., Lickliter, Jason D. and Boyd, Andrew W. (2013). EphA3 Maintains Tumorigenicity and Is a Therapeutic Target in Glioblastoma Multiforme. Cancer Cell, 23 (2), 238-248. doi: 10.1016/j.ccr.2013.01.007

Analysis of Mitochondrial Function and Localisation during Human Embryonic Stem Cell Differentiation In Vitro

Prowse, Andrew B. J., Chong, Fenny, Elliott, David A., Elefanty, Andrew G., Stanley, Edouard G., Gray, Peter P., Munro, Trent P. and Osborne, Geoffrey W. (2012). Analysis of Mitochondrial Function and Localisation during Human Embryonic Stem Cell Differentiation In Vitro. PLoS One, 7 (12) e52214, e52214-1-e52214-10. doi: 10.1371/journal.pone.0052214

Comprehensive transcriptome and immunophenotype analysis of renal and cardiac MSC-like populations supports strong congruence with bone marrow MSC despite maintenance of distinct identities (vol 8, pg 58, 2012)

Pelekanos, Rebecca A., Li, Joan, Gongora, Milena, Chandrakanthan, Vashe, Scown, Janelle, Suhaimi, Norseha, Brooke, Gary, Christensen, Melinda E., Doan, Tram, Rice, Alison M., Osborne, Geoffrey W., Grimmond, Sean M., Harvey, Richard P., Atkinson, Kerry and Little, Melissa H. (2012). Comprehensive transcriptome and immunophenotype analysis of renal and cardiac MSC-like populations supports strong congruence with bone marrow MSC despite maintenance of distinct identities (vol 8, pg 58, 2012). Stem Cell Research, 9 (1), 58-58. doi: 10.1016/j.scr.2012.02.003

Comprehensive transcriptome and immunophenotype analysis of renal and cardiac MSC-like populations supports strong congruence with bone marrow MSC despite maintenance of distinct identities

Pelekanos, Rebecca A., Li, Joan, Gongora, Milena, Chandrakanthan, Vashe, Scown, Janelle, Suhaimi, Norseha, Gary Brooke, Christensen, Melinda E., Doan, Tram, Rice, Alison M., Osborne, Geoffrey W., Grimmond, Sean M., Harvey, Richard P., Atkinson, Kerry and Little, Melissa H. (2012). Comprehensive transcriptome and immunophenotype analysis of renal and cardiac MSC-like populations supports strong congruence with bone marrow MSC despite maintenance of distinct identities. Stem Cell Research, 8 (1), 58-73. doi: 10.1016/j.scr.2011.08.003

Purification of immature neuronal cells from neural stem cell progeny

Azari, Hassan, Osborne, Geoffrey W., Yasuda, Takahiro, Golmohammadi, Mohammed G., Rahman, Maryam, Deleyroll, Loic P., Esfandiari, Ebrahim, Adams, David J., Scheffler, Bjorn, Steindler, Dennis A. and Reynolds, Brent A. (2011). Purification of immature neuronal cells from neural stem cell progeny. PLoS One, 6 (6) e20941, e20941-13053. doi: 10.1371/journal.pone.0020941

Evidence for label-retaining tumour-initiating cells in human glioblastoma

Deleyrolle, Loic P., Harding, Angus, Cato, Kathleen, Siebzehnrubl, Florian A., Rahman, Maryam, Azari, Hassan, Olson, Sarah, Gabrielli, Brian, Osborne, Geoffrey, Vescovi, Angelo and Reynolds, Brent A. (2011). Evidence for label-retaining tumour-initiating cells in human glioblastoma. Brain, 134 (5), 1331-1343. doi: 10.1093/brain/awr081

A method of quantifying cell sorting yield in "Real time"

Osborne, Geoffrey W. (2010). A method of quantifying cell sorting yield in "Real time". Cytometry Part A, 77A (10), 983-989. doi: 10.1002/cyto.a.20950

Cellular plasticity of inflammatory myeloid cells in the peritoneal foreign body response

Mooney, Jane E., Rolfe, Barbara E., Osborne, Geoffrey W., Sester, David P., van Rooijen, Nico, Campbell, Gordon R., Hume, David A. and Campbell, Julie H. (2010). Cellular plasticity of inflammatory myeloid cells in the peritoneal foreign body response. American Journal of Pathology, 176 (1), 369-380. doi: 10.2353/ajpath.2010.090545

Multiplexed Staining of Live Human Embryonic Stem Cells for Flow Cytometric Analysis of Pluripotency Markers

Prowse, ABJ, Wilson, J, Osborne, GW, Gray, PP and Wolvetang, EJ (2009). Multiplexed Staining of Live Human Embryonic Stem Cells for Flow Cytometric Analysis of Pluripotency Markers. Stem Cells and Development, 18 (8), 1135-1139. doi: 10.1089/scd.2009.0080

p75 neurotrophin receptor regulates basal and fluoxetine-stimulated hippocampal neurogenesis

Colditz, Michael J., Catts, Vibeke S., Al-menhali, Noura, Osborne, Geoffrey W., Bartlett, Perry F. and Coulson, Elizabeth J. (2009). p75 neurotrophin receptor regulates basal and fluoxetine-stimulated hippocampal neurogenesis. Experimental Brain Research, 200 (2), 161-167. doi: 10.1007/s00221-009-1947-6

Multiplexed labeling of viable cells for high-throughput analysis of glycine receptor function using flow cytometry

Gilbert, Daniel, Wilson, John C., Nink, Virginia, Lynch, Joseph W. and Osborne, Geoffrey W. (2009). Multiplexed labeling of viable cells for high-throughput analysis of glycine receptor function using flow cytometry. Cytometry Part A, 75A (5), 440-449. doi: 10.1002/cyto.a.20703

The GTP-binding protein G(i alpha) translocates to kinetochores and regulates the M-G(1) cell cycle transition of Swiss 3T3 cells

Crouch, MF, Osborne, GW and Willard, FS (2000). The GTP-binding protein G(i alpha) translocates to kinetochores and regulates the M-G(1) cell cycle transition of Swiss 3T3 cells. Cellular Signalling, 12 (3), 153-163. doi: 10.1016/S0898-6568(99)00080-7

Histo-cytometric analysis of prenatal mesencephalic dopaminergic terminal specification based on NURR1 and tyrosine hydroxylase

Luan, W., Alexander, S., Osborne, G., Nink, V., Cui, X. and Eyles, D. (2015). Histo-cytometric analysis of prenatal mesencephalic dopaminergic terminal specification based on NURR1 and tyrosine hydroxylase. 25th Biennial Meeting of the International Society for Neurochemistry Jointly with the 13th Meeting of the Asian Pacific Society for Neurochemistry in Conjunction with the 35th Meeting of the Australasian Neuroscience Society, Cairns, QLD Australia, 23-27 August 2015. Chichester, West Sussex United Kingdom: Wiley-Blackwell. doi: 10.1111/jnc.13188

Norepinephrine stimulates a distinct precursor population in the adult hippocampus

Jhaveri, Dhanisha, Newcombe, Estella, Nink, Virginia, Osborne, Geoffrey, Vaidya, Vidita and Bartlett, Perry (2012). Norepinephrine stimulates a distinct precursor population in the adult hippocampus. 19th Biennial Meeting of the International-Society-for-Developmental-Neuroscience (ISDN), Mumbai, India, 11-14 January 2012. Kidlington, Oxford, United Kingdom: Pergamon. doi: 10.1016/j.ijdevneu.2012.03.332

A frequency based method for sorting in flow cytometers

Osborne, G. and Ericksson, G. B. (2006). A frequency based method for sorting in flow cytometers. Stem Cells in the Age of Fluorescence Technology, Goldcoast, Queensland, Australia, November, 6-9, 2005. Hoboken: Wiley-Liss.

Recovery of sorted samples at given combinations of deflection plate voltage and droplet charge

Nink, V. and Osborne, G. (2006). Recovery of sorted samples at given combinations of deflection plate voltage and droplet charge. 7th Samuel A Latt Conference on Stem Cells in the Age of Fluorescence Technology, Gold Coast, Queensland, Australia, 6-9 November, 2005. Hoboken: Wiley-Liss. doi: 10.1002/cyto.a.20264

Accurate cell sorting and positioning studies

Osborne, G. W. (2004). Accurate cell sorting and positioning studies. 27th Annual Meeting of the Australasian Flow Cytometry Group, Adelaide Convention Centre, 12-16 December 2004. Adelaide Australia: Australasian Flow Cytometry Group.

The GTP-binding protein Gi alpha 2 translocates to kinetochores and regulates the M-G1 transition of Swiss 3T3 cells.

Willard, FS, Osborne, GW and Crouch, MF (2000). The GTP-binding protein Gi alpha 2 translocates to kinetochores and regulates the M-G1 transition of Swiss 3T3 cells..

Nitric Oxide and Malarial Immunosuppression

Rockett, KA, Awburn, MM, Rockett, EJ, Cowden, WB, Osborne, GW, Rolph, MS and Clark, IA (1994). Nitric Oxide and Malarial Immunosuppression. 3rd International Meeting on the Biology of Nitric Oxide, Cologne Germany, Oct 04-06, 1993.