The first integrated multimodal assay for the ultrasensitive detection of dengue contamination of blood (2012–2015)

Abstract:
For the first time we will develop an ultrasensitive system that will simultaneously detect validated nucleic acid, protein & serological biomarkers of dengue in a single assay format suitable for the screening of blood. Isothermally-amplified viral nucleic acid, NS1 antigen & IgM antibodies will be captured & concentrated using magnetic nanoparticles functionalised with state-of-the-art polymer chemistry which reduces non-specific binding. The captured dengue markers will then be probed with individually-tagged fluorescent beads in a format amenable to automation & therefore suitable for blood bank application.
Grant type:
ARC Linkage Projects
Researchers:
  • Professorial Research Fellow - GL
    Institute for Molecular Bioscience
  • Head of School
    School of Chemistry and Molecular Biosciences
    Faculty of Science
    Affiliated Professor
    Institute for Molecular Bioscience
    Affiliate Professor
    Australian Institute for Bioengineering and Nanotechnology
  • Senior Group Leader
    Australian Institute for Bioengineering and Nanotechnology
Funded by:
Australian Research Council