The first integrated multimodal assay for the ultrasensitive detection of dengue contamination of blood (2012–2015)
For the first time we will develop an ultrasensitive system that will simultaneously detect validated nucleic
acid, protein & serological biomarkers of dengue in a single assay format suitable for the screening of blood. Isothermally-amplified viral nucleic acid, NS1 antigen & IgM antibodies will be captured & concentrated using magnetic nanoparticles functionalised with state-of-the-art polymer chemistry which reduces non-specific binding. The captured dengue markers will then be probed with individually-tagged fluorescent beads in a format amenable to automation & therefore suitable for blood bank application.