All-in-vitro engineering and single molecule analysis of protein complexes

Abstract:: Understanding the structure and dynamics of protein interaction networks is the greatest challenge of modern biology. We will combine single-molecule spectroscopy with in vitro protein expression in microfluidic chips to create a novel technology for the engineering and characterisation of protein complexes. It will be applied to eukaryotic membrane- modelling protein assemblies, such as the Cavin and SNX-BAR complexes. The single-molecule analysis of protein-protein interactions will shed light on the topology and assembly mechanisms of these complexes. The developed technologies will enable rapid progress in structure-function investigations of very important protein complexes such as the spliceosome, the nucleopore complex, and viruses.
Grant type:: ARC Discovery Projects
Funded by:: Australian Research Council

The University of Queensland