Transcriptional regulation of erythropoiesis (2007–2009)
Scientists know the complete sequence of the genome. The current challenge is to work out how it works. The identity of each cell in a complex organism is determined by the genes it chooses to use as a blueprint for making proteins, and those it chooses to ignore. Selective gene usage is driven by control proteins which bind to regulatory regions of wanted genes to activate them, or unwanted genes to silence them. This proposal will use genetics, genomics and bioinformatics to piece together how two key zinc finger master control proteins (EKLF and GATA-1) work together within red cells. Our expected outcome is to generate a global roadmap for future studies into how the genome works in any cell.