High-throughput DNA sequencing technologies have revolutionised microbiology in recent years. It is now possible to determine the entire genetic blueprint of a bacterium, its genome, accurately, quickly and cheaply. A recent innovation is single-molecule real-time sequencing which provides a complete read-out of a bacterial genome (genetic data) along with chemical modifications of the DNA (epigenetic data). Epigenetic data can impact regulation: how genes are switched off and on. This project seeks to harness the power of single-molecule DNA sequencing, together with state-of-the-art genomic and molecular approaches, to better understand the impact of DNA modification on gene regulation in the model bacterial organism, Escherichia coli.