Sequencing and assembling microbial community metagenomes in real-time (2017–2019)
This Project aims to develop methodologies to assemble metagenomes directly from complex environmental samples in real-time using nanopore sequencing. Current short-read approaches to metagenomics are unable to assemble mixed genomes from an environmental sample, and hence focus on describing which species and genes are present. The recent development of long-read nanopore sequencing introduces the potential to assemble full genomes of multiple species present in a sample. By enabling assembly of complete genomes in important resources such as water and soil, our approach will facilitate a much deeper understanding of the dynamics, variation and transfer of genetic material within microbial communities in these resources.