Correct left-right (LR) patterning of the body is crucial for correct organ function and therefore survival. How the heart interprets these signals is the subject of this application. The heart begins in the embryo as a symmetrical sheet of cells exposed to signals from the LR axis. Cells reorganise to form an asymmetric linear tube followed by twisting to form the looped heart. The project will investigate how the heart responds to LR signals; which tissue is dominant in this response; determine tissue intrinsic factors at play, and; determine whether we can manipulate this. Precision quantification, cytoskeletal analysis, laser and microsurgery will be performed to address when and how the heart acquires laterality.