Automated high resolution & high contrast cryoTEM for 3D structural biology (2018)

Abstract:
The aim of this proposal is to establish a frontier facility in automated single particle cryo-TEM and cryo-TEM tomography (Titan Krios) that will enable state-of-the-art atomic molecular structure research and 3D subcellular and cellular imaging for Australian scientists. It will span all multiscale cryo-TEM modalities from the visualisation of cells, membranes and macromolecular complexes, through to near-atomic resolution protein structure determination. With more than 200 new protein structures in 2016 achieved at 2-5Å resolution by the Titan Krios, cryo-single particle analysis and tomography are recognised as revolutionary technologies in molecular structural biology and powerful enablers of future ground-breaking discovery.
Grant type:
ARC Linkage Infrastructure, Equipment and Facilities
Researchers:
  • Professor
    School of Chemistry and Molecular Biosciences
    Faculty of Science
    Affil Professorial Research Fellow
    Institute for Molecular Bioscience
  • Director
    Centre for Microscopy and Microanalysis
    Faculty of Science
    Affiliated Professor
    School of Chemistry and Molecular Biosciences
    Faculty of Science
  • Professorial Research Fellow
    Institute for Molecular Bioscience
    Affiliate Professor
    School of Biomedical Sciences
    Faculty of Medicine
  • Professorial Research Fellow
    Institute for Molecular Bioscience
  • Senior Lecturer
    School of Chemistry and Molecular Biosciences
    Faculty of Science
    Affiliate Research Fellow
    Institute for Molecular Bioscience
Funded by:
Australian Research Council