Dr Dmitry Ovchinnikov

Research Fellow - StemCore

Australian Institute for Bioengineering and Nanotechnology
d.ovchinnikov@uq.edu.au
+61 7 334 63472

Overview

Dmitry Ovchinnikov is currently a scientific director and general manager of the NCRIS/BPA-funded stem cell functional genomics facility, StemCore, and a Research Fellow at the Australian Institute for Bioengineering and Nanotechnology at the University of Queensland. His main research interests lie in the genetic manipulation of pluripotent stem cells for investigations into the stem cell biology and include the generation of transgenic ESC and iPSC research tools for bioengineering applications, re-programming somatic cells to pluripotency (iPSC generation), seamless (traceless) correction of the disease-causing mutations, and other precise modifications of the human genome. More recently, he has also developed an interest in the transcriptomics and epigenetics of pluripotent cells, their derivatives and cancer cells. Previously, he led the Transgenic subgroup in the laboratory of Professor David Hume at UQ’s Institute for Molecular Biosciences, where he generated a number of transgenic mouse lines, some of which are widely used for investigations into macrophage function, and other in vivo studies. Dmitry received his Diploma in Biochemistry (Masters equivalent) from the Lomonosov Moscow State University in 1993. He completed his PhD studies in the laboratory of Professor Richard R. Behringer at the University of Texas MD Anderson Cancer Center, where he was involved in the development of transgenic resources and performed some of the first studies in mice utilising the conditional knockout-enabling Cre/loxP system.

Research Interests

  • Molecular genetics of human pluripotent stem cells
    My research interests revolve around genetic and epigenetic regulation of normal pluripotent stem cell maintenance and differentiation as well as mechanisms of development and progression of human genetically-based diseases (syndromes) and cancer. In particular, I have been involved in development of numerous transgenic human ES/iPS tools facilitating studies of both maintenance of pluripotency and directed differentiation, with particular focus on neural and cardiac cell development. I'm also creating platform technologies allowing for both up- and down-modulation of the function of single and groups of genes. Those include inducible gene overexpression systems and regulatable system for generation of pre-determined genomic alterations in pluripotent stem cells.

Research Impacts

In July, 2017 assumed leadership of the StemCore, a Stem Cells Limited / NCRIS(BPA) / UQ AIBN stem cell functional genomics facility

Research metrics: h index 24 (Nov 2019), i10 index 35 (over 5200 citations)

Invited participant in the Australian Academy of Sciences'/ The Royal Society's (UK) Theo Murphy High Flyers Think Tank - "The stem cell revolution: Lessons and imperatives for Australia", Sydney, July 22-24 2015

Dmitry delivered talks at many of the top stem cell research-shaping meetings, including regular ISSCR and ASSCR meetings.

Qualifications

  • Doctor of Philosophy, University of California

Publications

  • Alateeq, Suad, Ovchinnikov, Dmitry, Tracey, Timothy, Whitworth, Deanne, Al-Rubaish, Abdullah, Al-Ali, Amein and Wolvetang, Ernst (2018). Identification of on-target mutagenesis during correction of a beta-thalassemia splice mutation in iPS cells with optimised CRISPR/Cas9-double nickase reveals potential safety concerns. APL Bioengineering, 2 (4) doi:10.1063/1.5048625

  • Ovchinnikov, Dmitry A., Korn, Othmar, Virshup, Isaac, Wells, Christine A. and Wolvetang, Ernst J. (2018). The impact of APP on Alzheimer-like pathogenesis and gene expression in Down syndrome iPSC-derived neurons. Stem Cell Reports, 11 (1) 32-42. doi:10.1016/j.stemcr.2018.05.004

  • Bellmaine, Stephanie F., Ovchinnikov, Dmitry A., Manallack, David T., Cuddy, Claire E., Elefanty, Andrew G., Stanley, Edouard G., Wolvetang, Ernst J., Williams, Spencer J. and Pera, Martin (2017). Inhibition of DYRK1A disrupts neural lineage specificationin human pluripotent stem cells. Elife, 6e24502, doi:10.7554/eLife.24502

  • Ovchinnikov, Dmitry A., Sun, Jane and Wolvetang, Ernst J. (2015). Generation of footprint-free induced pluripotent stem cells from human fibroblasts using episomal plasmid vectors. Methods in Molecular Biology, 133037-45. doi:10.1007/978-1-4939-2848-4_4

  • Arner, Erik, Daub, Carsten O., Vitting-Seerup, Kristoffer, Andersson, Robin, Lilje, Berit, Drabløs, Finn, Lennartsson, Andreas, Rönnerblad, Michelle, Hrydziuszko, Olga, Vitezic, Morana, Freeman, Tom C., Alhendi, Ahmad M. N., Arner, Peter, Axton, Richard, Baillie, J. Kenneth, Beckhouse, Anthony, Bodega, Beatrice, Briggs, James, Brombacher, Frank, Davis, Margaret, Detmar, Michael, Ehrlund, Anna, Endoh, Mitsuhiro, Eslami, Afsaneh, Fagiolini, Michela, Fairbairn, Lynsey, Faulkner, Geoffrey J., Ferrai, Carmelo, Fisher, Malcolm E., Forrester, Lesley and Goldowitz, Daniel (2015). Transcribed enhancers lead waves of coordinated transcription in transitioning mammalian cells. Science, 347 (6225) 1010-1014. doi:10.1126/science.1259418

  • Ovchinnikov, Dmitry A.,, Hidalgo, Alejandro, Yang, Seung-Kwon, Zhang, Xinli, Hudson, James, Mazzone, Stuart B., Chen, Chen, Cooper-White, Justin J. and Wolvetang, Ernst J. (2014). Isolation of contractile cardiomyocytes from human pluripotent stem-cell-derived cardiomyogenic cultures using a human NCX1-EGFP reporter. Stem Cells and Development, 24 (1) 11-20. doi:10.1089/scd.2014.0195

  • Ovchinnikov, Dmitry A., Titmarsh, Drew M., Fortuna, Patrick R. J., Hidalgo, Alejandro, Alharbi, Samah, Whitworth, Deanne J., Cooper-White, Justin J. and Wolvetang, Ernst J. (2014). Transgenic human ES and iPS reporter cell lines for identification and selection of pluripotent stem cells in vitro. Stem Cell Research, 13 (2) 251-261. doi:10.1016/j.scr.2014.05.006

  • Forest, Alistair R. R., Kawaji, Hideya, Rehli, Michael, Baillie, J. Kenneth, De Hoon, Michael J. L., Haberle, Vanja, Lassmann, Timo, Kulakovskiy, Ivan V., Lizio, Marina, Itoh, Masayoshi, Andersson, Robin, Mungall, Christopher J., Meehan, Terrence F., Schmeier, Sebastian, Bertin, Nicholas, Jorgensen, Mette, Dimont, Emmanuel, Arner, Erik, Schmidl, Christian, Schaefer, Ulf, Medvedeva, Yulia A., Plessy, Charles, Vitezic, Morana, Severin, Jessica, Semple, Colin A., Ishizu, Yuri, Young, Robert S., Francescatto, Margherita, Altschuler, Intikhab, Albanese, Davide and Altschule, Gabriel M. (2014). A promoter-level mammalian expression atlas. Nature, 507 (7493) 462-470. doi:10.1038/nature13182

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Note for students: The possible research projects listed on this page may not be comprehensive or up to date. Always feel free to contact the staff for more information, and also with your own research ideas.