The University of Queensland

Spatial transcriptomics in kidney tissue

Raghubar, Arti M., Crawford, Joanna, Jones, Kahli, Lam, Pui Y., Andersen, Stacey B., Matigian, Nicholas A., Ng, Monica S Y, Healy, Helen, Kassianos, Andrew J. and Mallett, Andrew J. (2023). Spatial transcriptomics in kidney tissue. Kidney research: experimental protocols. (pp. 233-282) edited by Tim D. Hewitson, Nigel D. Toussaint and Edward R. Smith. New York, NY USA: Humana New York. doi: 10.1007/978-1-0716-3179-9_17

Isolation of Human Blood DC Subtypes

Kassianos, Andrew J., Jongbloed, Sarah L., Hart, Derek N.J. and Radford, Kristen J. (2010). Isolation of Human Blood DC Subtypes. Dendritic cell protocols. (pp. 45-54) edited by Shalin H. Naik. New York, NY United States: Humana Press. doi: 10.1007/978-1-60761-421-0_3

Human proximal tubular epithelial cell-derived small extracellular vesicles mediate synchronized tubular ferroptosis in hypoxic kidney injury

Wang, Xiangju, Kim, Chang Seong, Adams, Benjamin C., Wilkinson, Ray, Hill, Michelle M., Shah, Alok K., Mohamed, Ahmed, Dutt, Mriga, Ng, Monica S.Y., Ungerer, Jacobus P.J., Healy, Helen G. and Kassianos, Andrew J. (2024). Human proximal tubular epithelial cell-derived small extracellular vesicles mediate synchronized tubular ferroptosis in hypoxic kidney injury. Redox Biology, 70 103042, 103042. doi: 10.1016/j.redox.2024.103042

High risk clear cell renal cell carcinoma microenvironments contain protumour immunophenotypes lacking specific immune checkpoints

Raghubar, Arti M., Matigian, Nicholas A., Crawford, Joanna, Francis, Leo, Ellis, Robert, Healy, Helen G., Kassianos, Andrew J., Ng, Monica S. Y., Roberts, Matthew J., Wood, Simon and Mallett, Andrew J. (2023). High risk clear cell renal cell carcinoma microenvironments contain protumour immunophenotypes lacking specific immune checkpoints. npj Precision Oncology, 7 (1) 88, 1-9. doi: 10.1038/s41698-023-00441-5

Minimal change disease with Jack jumper ant stings: A case report

Tunbridge, Matthew, Grivei, Anca, Kassianos, Andrew J., Davis, Helen, Stewart, Anne, Healy, Helen, Mon, Saw Yu and John, George T. (2022). Minimal change disease with Jack jumper ant stings: A case report. Nephrology, 27 (11), 908-909. doi: 10.1111/nep.14104

Cellular milieu in clear cell renal cell carcinoma

Raghubar, Arti M., Roberts, Matthew J., Wood, Simon, Healy, Helen G., Kassianos, Andrew J. and Mallett, Andrew J. (2022). Cellular milieu in clear cell renal cell carcinoma. Frontiers in Oncology, 12 943583, 1-10. doi: 10.3389/fonc.2022.943583

Hypoxic human proximal tubular epithelial cells undergo ferroptosis and elicit an NLRP3 inflammasome response in CD1c+ dendritic cells

Giuliani, Kurt T. K., Grivei, Anca, Nag, Purba, Wang, Xiangju, Rist, Melissa, Kildey, Katrina, Law, Becker, Ng, Monica S., Wilkinson, Ray, Ungerer, Jacobus, Forbes, Josephine M., Healy, Helen and Kassianos, Andrew J. (2022). Hypoxic human proximal tubular epithelial cells undergo ferroptosis and elicit an NLRP3 inflammasome response in CD1c+ dendritic cells. Cell Death and Disease, 13 (8) 739, 1-13. doi: 10.1038/s41419-022-05191-z

Spatially resolved transcriptomes of mammalian kidneys illustrate the molecular complexity and interactions of functional nephron segments

Raghubar, Arti M., Pham, Duy T., Tan, Xiao, Grice, Laura F., Crawford, Joanna, Lam, Pui Yeng, Andersen, Stacey B., Yoon, Sohye, Teoh, Siok Min, Matigian, Nicholas A., Stewart, Anne, Francis, Leo, Ng, Monica S. Y., Healy, Helen G., Combes, Alexander N., Kassianos, Andrew J., Nguyen, Quan and Mallett, Andrew J. (2022). Spatially resolved transcriptomes of mammalian kidneys illustrate the molecular complexity and interactions of functional nephron segments. Frontiers in Medicine, 9 873923, 873923. doi: 10.3389/fmed.2022.873923

Promoting plant-based therapies for chronic kidney disease

Khan, Muhammad Ali, Kassianos, Andrew J., Hoy, Wendy E., Alam, AHM Khurshid, Healy, Helen G. and Gobe, Glenda C. (2022). Promoting plant-based therapies for chronic kidney disease. Journal of Evidence-Based Integrative Medicine, 27, 2515690X2210796. doi: 10.1177/2515690x221079688

SARS-CoV-2 vaccination–associated collapsing glomerulopathy in a kidney transplant recipient

Jefferis, Julia, Kassianos, Andrew J., Grivei, Anca, Doucet, Brian, Healy, Helen, Francis, Leo, Mon, Saw Yu and John, George T. (2022). SARS-CoV-2 vaccination–associated collapsing glomerulopathy in a kidney transplant recipient. Kidney International, 101 (3), 635-636. doi: 10.1016/j.kint.2021.12.018

Adenine overload induces ferroptosis in human primary proximal tubular epithelial cells

Khan, Muhammad Ali, Nag, Purba, Grivei, Anca, Giuliani, Kurt T. K., Wang, Xiangju, Diwan, Vishal, Hoy, Wendy, Healy, Helen, Gobe, Glenda and Kassianos, Andrew J. (2022). Adenine overload induces ferroptosis in human primary proximal tubular epithelial cells. Cell Death and Disease, 13 (2) 104, 1-12. doi: 10.1038/s41419-022-04527-z

T cell expression and release of kidney injury molecule-1 in response to glucose variations initiates kidney injury in early diabetes

Forbes, Josephine M., McCarthy, Domenica A., Kassianos, Andrew J., Baskerville, Tracey, Fotheringham, Amelia J., Giuliani, Kurt T. K., Grivei, Anca, Murphy, Andrew J., Flynn, Michelle C., Sullivan, Mitchell A., Chandrashekar, Preeti, Whiddett, Rani, Radford, Kristen J., Flemming, Nicole, Beard, Sam S., D’Silva, Neisha, Nisbet, Janelle, Morton, Adam, Teasdale, Stephanie, Russell, Anthony, Isbel, Nicole, Jones, Timothy, Couper, Jennifer, Healy, Helen, Harris, Mark, Donaghue, Kim, Johnson, David W., Cotterill, Andrew, Barrett, Helen L. and O’Moore-Sullivan, Trisha (2021). T cell expression and release of kidney injury molecule-1 in response to glucose variations initiates kidney injury in early diabetes. Diabetes, 70 (8), 1754-1766. doi: 10.2337/db20-1081

Molecular and functional profiling of apical versus basolateral small extracellular vesicles derived from primary human proximal tubular epithelial cells under inflammatory conditions

Wang, Xiangju, Wilkinson, Ray, Kildey, Katrina, Ungerer, Jacobus P. J., Hill, Michelle M., Shah, Alok K., Mohamed, Ahmed, Dutt, Mriga, Molendijk, Jeffrey, Healy, Helen and Kassianos, Andrew J. (2021). Molecular and functional profiling of apical versus basolateral small extracellular vesicles derived from primary human proximal tubular epithelial cells under inflammatory conditions. Journal of Extracellular Vesicles, 10 (4) e12064, e12064. doi: 10.1002/jev2.12064

Oxidative stress and inflammasome activation in human rhabdomyolysis-induced acute kidney injury

Grivei, Anca, Giuliani, Kurt T.K., Wang, Xiangju, Ungerer, Jacobus, Francis, Leo, Hepburn, Kirsten, John, George T., Gois, Pedro F.H., Kassianos, Andrew J. and Healy, Helen (2020). Oxidative stress and inflammasome activation in human rhabdomyolysis-induced acute kidney injury. Free Radical Biology and Medicine, 160, 690-695. doi: 10.1016/j.freeradbiomed.2020.09.011

The emerging role of renal tubular epithelial cells in the immunological pathophysiology of lupus nephritis

Hong, Seokchan, Healy, Helen and Kassianos, Andrew J. (2020). The emerging role of renal tubular epithelial cells in the immunological pathophysiology of lupus nephritis. Frontiers in Immunology, 11 578952, 578952. doi: 10.3389/fimmu.2020.578952

The role of inflammation and inflammasome activation in human bile cast nephropathy

Giuliani, Kurt T. K., Kassianos, Andrew J., Kildey, Katrina, Grivei, Anca, Wang, Xiangju, Ungerer, Jacobus, Francis, Leo, Healy, Helen and Gois, Pedro F. H. (2020). The role of inflammation and inflammasome activation in human bile cast nephropathy. Nephrology, 25 (6) nep.13696, 502-506. doi: 10.1111/nep.13696

Underlying histopathology determines response to oxidative stress in cultured human primary proximal tubular epithelial cells

Khan, Muhammad Ali, Wang, Xiangju, Giuliani, Kurt T. K., Nag, Purba, Grivei, Anca, Ungerer, Jacobus, Hoy, Wendy, Healy, Helen, Gobe, Glenda and Kassianos, Andrew J. (2020). Underlying histopathology determines response to oxidative stress in cultured human primary proximal tubular epithelial cells. International Journal of Molecular Sciences, 21 (2) 560, 1-15. doi: 10.3390/ijms21020560

Specialized roles of human natural killer cell subsets in kidney transplant rejection

Kildey, Katrina, Francis, Ross S., Hultin, Sebastian, Harfield, Michelle, Giuliani, Kurt, Law, Becker M. P., Wang, Xiangju, See, Emily J., John, George, Ungerer, Jacobus, Wilkinson, Ray, Kassianos, Andrew J. and Healy, Helen (2019). Specialized roles of human natural killer cell subsets in kidney transplant rejection. Frontiers in Immunology, 10 (AUG) Article 1877, 1877. doi: 10.3389/fimmu.2019.01877

Human tissue-resident mucosal-associated invariant T (MAIT) cells in renal fibrosis and CKD

Law, Becker M. P., Wilkinson, Ray, Wang, Xiangju, Kildey, Katrina, Giuliani, Kurt, Beagley, Kenneth W., Ungerer, Jacobus, Healy, Helen and Kassianos, Andrew J. (2019). Human tissue-resident mucosal-associated invariant T (MAIT) cells in renal fibrosis and CKD. Journal of the American Society of Nephrology, 30 (7), 1322-1335. doi: 10.1681/asn.2018101064

Biomarkers and the role of mast cells as facilitators of inflammation and fibrosis in chronic kidney disease

Owens, Evan P., Vesey, David A., Kassianos, Andrew J., Healy, Helen, Hoy, Wendy E. and Gobe, Glenda C. (2019). Biomarkers and the role of mast cells as facilitators of inflammation and fibrosis in chronic kidney disease. Translational Andrology and Urology, 8 (S2), S175-S183. doi: 10.21037/tau.2018.11.03

Pigment nephropathy: novel insights into inflammasome-mediated pathogenesis

Giuliani, Kurt T. K., Kassianos, Andrew J., Healy, Helen and Gois, Pedro H. F. (2019). Pigment nephropathy: novel insights into inflammasome-mediated pathogenesis. International Journal of Molecular Sciences, 20 (8) 1997, 1997. doi: 10.3390/ijms20081997

Natural killer cells in kidney health and disease

Turner, Jan-Eric, Rickassel, Constantin, Healy, Helen and Kassianos, Andrew J. (2019). Natural killer cells in kidney health and disease. Frontiers in Immunology, 10 (March) 587. doi: 10.3389/fimmu.2019.00587

The role of oxidative stress and inflammation in acute oxalate nephropathy associated with ethylene glycol intoxication

Wilson, Gregory J., Gois, Pedro H.F., Zhang, Alice, Wang, Xiangju, Law, Becker M.P., Kassianos, Andrew J. and Healy, Helen G. (2018). The role of oxidative stress and inflammation in acute oxalate nephropathy associated with ethylene glycol intoxication. Kidney International Reports, 3 (5), 1217-1221. doi: 10.1016/j.ekir.2018.05.005

Identification and quantitation of leukocyte populations in human kidney tissue by multi-parameter flow cytometry

Kildey, Katrina, Law, Becker M. P., Muczynski, Kimberly A., Wilkinson, Ray, Healy, Helen and Kassianos, Andrew J. (2018). Identification and quantitation of leukocyte populations in human kidney tissue by multi-parameter flow cytometry. Bio-Protocol, 8 (16), e2980. doi: 10.21769/BioProtoc.2980

Effector γδ T cells in human renal fibrosis and chronic kidney disease

Law, Becker Meng-Po, Wilkinson, Ray, Wang, Xiangju, Kildey, Katrina, Lindner, Mae, Beagley, Kenneth, Healy, Helen and Kassianos, Andrew J. (2018). Effector γδ T cells in human renal fibrosis and chronic kidney disease. Nephrology, Dialysis, Transplantation, 34 (1), 40-48. doi: 10.1093/ndt/gfy098

Interferon-γ production by tubulointerstitial human CD56bright natural killer cells contributes to renal fibrosis and chronic kidney disease progression

Law, Becker M. P., Wilkinson, Ray, Wang, Xiangju, Kildey, Katrina, Lindner, Mae, Rist, Melissa J., Beagley, Kenneth, Healy, Helen and Kassianos, Andrew J. (2017). Interferon-γ production by tubulointerstitial human CD56bright natural killer cells contributes to renal fibrosis and chronic kidney disease progression. Kidney International, 92 (1), 79-88. doi: 10.1016/j.kint.2017.02.006

Unique molecular profile of exosomes derived from primary human proximal tubular epithelial cells under diseased conditions

Wang, Xiangju, Wilkinson, Ray, Kildey, Katrina, Potriquet, Jeremy, Mulvenna, Jason, Lobb, Richard J., Moller, Andreas, Cloonan, Nicole, Mukhopadhyay, Pamela, Kassianos, Andrew J. and Healy, Helen (2017). Unique molecular profile of exosomes derived from primary human proximal tubular epithelial cells under diseased conditions. Journal of Extracellular Vesicles, 6 (1) 1314073, 1314073-1314073. doi: 10.1080/20013078.2017.1314073

Human proximal tubule epithelial cells modulate autologous B-cell function

Sampangi, Sandeep, Wang, Xiangju, Beagley, Kenneth W., Klein, Travis, Afrin, Sadia, Healy, Helen, Wilkinson, Ray and Kassianos, Andrew J. (2015). Human proximal tubule epithelial cells modulate autologous B-cell function. Nephrology Dialysis Transplantation, 30 (10), 1674-1683. doi: 10.1093/ndt/gfv242

The mechanisms of human renal epithelial cell modulation of autologous dendritic cell phenotype and function

Sampangi, Sandeep, Kassianos, Andrew J., Wang, Xiangju, Beagley, Kenneth W., Klein, Travis, Afrin, Sadia, Healy, Helen and Wilkinson, Ray (2015). The mechanisms of human renal epithelial cell modulation of autologous dendritic cell phenotype and function. PLoS One, 10 (7) e0134688, e0134688. doi: 10.1371/journal.pone.0134688

Fractalkine-CX3CR1-dependent recruitment and retention of human CD1c+ myeloid dendritic cells by in vitro-activated proximal tubular epithelial cells

Kassianos, Andrew J., Wang, Xiangju, Sampang, Sandeep, Afrin, Sadia, Wilkinson, Ray and Healy, Helen (2015). Fractalkine-CX3CR1-dependent recruitment and retention of human CD1c+ myeloid dendritic cells by in vitro-activated proximal tubular epithelial cells. Kidney International, 87 (6), 1153-1163. doi: 10.1038/ki.2014.407

Differential uptake and cross-presentation of soluble and necrotic cell antigen by human DC subsets

Chiang, Meng-Chieh, Tullett, Kirsteen M., Lee, Yoke Seng, Idris, Adi, Ding, Yitian, McDonald, Kylie J., Kassianos, Andrew, Leal Rojas, Ingrid M., Jeet, Varinder, Lahoud, Mireille H. and Radford, Kristen J. (2015). Differential uptake and cross-presentation of soluble and necrotic cell antigen by human DC subsets. European Journal of Immunology, 46 (2), 329-339. doi: 10.1002/eji.201546023

Laser capture microdissection and multiplex-tandem PCR analysis of proximal tubular epithelial cell signaling in human kidney disease

Wilkinson, Ray, Wang, Xiangju, Kassianos, Andrew J., Zuryn, Steven, Roper, Kathrein E., Osborne, Andrew, Sampangi, Sandeep, Francis, Leo, Raghunath, Vishwas and Healy, Helen (2014). Laser capture microdissection and multiplex-tandem PCR analysis of proximal tubular epithelial cell signaling in human kidney disease. PLoS One, 9 (1) e87345, e87345.1-e87345.10. doi: 10.1371/journal.pone.0087345

Increased tubulointerstitial recruitment of human CD141hi CLEC9A+ and CD1c+ myeloid dendritic cell subsets in renal fibrosis and chronic kidney disease

Kassianos, Andrew J., Wang, Xiangju, Sampangi, Sandeep, Muczynski, Kimberly, Healy, Helen and Wilkinson, Ray (2013). Increased tubulointerstitial recruitment of human CD141hi CLEC9A+ and CD1c+ myeloid dendritic cell subsets in renal fibrosis and chronic kidney disease. American Journal of Physiology - Renal Physiology, 305 (10), F1391-F1401. doi: 10.1152/ajprenal.00318.2013

Indoleamine 2,3-dioxygenase activity contributes to local immune suppression in the skin expressing human papillomavirus oncoprotein E7

Mittal, Deepak, Kassianos, Andrew. J, Tran, Lee S., Bergot, Anne-Sophie, Gosmann, Christina, Hofmann, Janin, Blumenthal, Antje, Leggatt, Graham R. and Frazer, Ian H. (2013). Indoleamine 2,3-dioxygenase activity contributes to local immune suppression in the skin expressing human papillomavirus oncoprotein E7. Journal of Investigative Dermatology, 133 (12), 2686-2694. doi: 10.1038/jid.2013.222

Human proximal tubule epithelial cells modulate autologous dendritic cell function

Kassianos, Andrew J., Sampangi, Sandeep, Wang, Xiangju, Roper, Kathrein E., Beagley, Ken, Healy, Helen and Wilkinson, Ray (2013). Human proximal tubule epithelial cells modulate autologous dendritic cell function. Nephrology Dialysis Transplantation, 28 (2), 303-312. doi: 10.1093/ndt/gfs136

Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli

Kassianos, Andrew J., Hardy, Melinda Y., Ju, Xinsheng, Vijayan, Dipti, Ding, Yitian, Vulink, Annelie J. E., McDonald, Kylie J., Jongbloed, Sarah L., Wadley, Robert B., Wells, Christine, Hart, Derek N. J. and Radford, Kristen J. (2012). Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli. European Journal of Immunology, 42 (6), 1512-1522. doi: 10.1002/eji.201142098

New approaches to immunotherapy for HPV associated cancer

Bergot, Anne-Sophie, Kassianos, Andrew, Frazer, Ian H. and Mittal, Deepak (2011). New approaches to immunotherapy for HPV associated cancer. Cancers, 3 (3), 3461-3495. doi: 10.3390/cancers3033461

CD34+ cord blood DC-induced antitumor lymphoid cells have efficacy in a murine xenograft model of human ALL

Cullup, Hannah, Hsu, Andy K. W., Kassianos, Andrew J., McDonald, Kylie, Radford, Kristen J. and Rice, Alison M. (2011). CD34+ cord blood DC-induced antitumor lymphoid cells have efficacy in a murine xenograft model of human ALL. Journal of Immunotherapy, 34 (4), 362-371. doi: 10.1097/CJI.0b013e31821b7230

Human CD141(+) (BDCA-3)(+) dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens

Jongbloed, Sarah L., Kassianos, Andrew J., McDonald, Kylie J., Clark, Georgina J., Ju, Xinsheng, Angel, Catherine E., Chen, Chun-Jen J., Dunbar, P. Rod, Wadley, obert B., Jeet, Varinder, Vulink, Annelie J.E., Hart, Derek N.J. and Radford, Kristen J. (2010). Human CD141(+) (BDCA-3)(+) dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens. Journal of Experimental Medicine, 207 (6), 1247-1260. doi: 10.1084/jem.20092140

NK cells enhance the induction of CTL responses by IL-15 monocyte-derived dendritic cells

Hardy, M., Kassianos, A., Vulink, A., Wilkinson, R., Jongbloed, S. L., Hart, D. N. J. and Radford, K. J. (2009). NK cells enhance the induction of CTL responses by IL-15 monocyte-derived dendritic cells. Immunology and Cell Biology, 87 (8), 606-614. doi: 10.1038/icb.2009.44

Numerical and functional assessment of blood dendritic cells in prostate cancer patients

Wilkinson, Ray, Kassianos, Andrew J., Swindle, Peter, Hart, Derek N. J. and Radford, Kristen J. (2006). Numerical and functional assessment of blood dendritic cells in prostate cancer patients. The Prostate, 66 (2), 180-192. doi: 10.1002/pros.20333

CD11c+ blood dendritic cells induce antigen-specific cytotoxic T lymphocytes with similar efficiency compared to monocyte-derived dendritic cells despite higher levels of MHC class I expression

Radford, Kristen J., Turtle, Cameron J., Kassianos, Andrew J. and Hart, Derek N. J. (2006). CD11c+ blood dendritic cells induce antigen-specific cytotoxic T lymphocytes with similar efficiency compared to monocyte-derived dendritic cells despite higher levels of MHC class I expression. Journal of Immunotherapy, 29 (6), 596-605. doi: 10.1097/01.cji.0000211310.90621.5d

Immunoselection of functional CMRF-56+ blood dendritic cells from multiple myeloma patients for immunotherapy

Radford, Kristen J., Turtle, Cameron J., Kassianos, Andrew J., Vuckovic, Slavica, Gardiner, Damien, Khalil, Dahlia, Taylor, Kerry, Wright, Sue, Gill, Devinder and Hart, Derek N. J. (2005). Immunoselection of functional CMRF-56+ blood dendritic cells from multiple myeloma patients for immunotherapy. Journal of Immunotherapy, 28 (4), 322-331. doi: 10.1097/01.cji.0000163592.66910.e4

Simple, robust strategies for generating DNA-directed RNA interference constructs

Rice, Robert R., Muirhead, Andrew N., Harrison, Bruce T., Kassianos, Andrew J., Sedlak, Petra L., Maugeri, Narelle J., Goss, Peter J., Davey, Jonathan R., James, David E. and Graham, Michael W. (2005). Simple, robust strategies for generating DNA-directed RNA interference constructs. Methods in Enzymology, 392 (18), 405-419. doi: 10.1016/S0076-6879(04)92024-1

Hypoxia and interleukin (IL)-1β synergistically induce cellular senescence in human proximal tubular epithelial cells (PTEC)

Kassianos, Andrew J., Giuliani, Kurt, Nag, Purba, Hong, Seokchan, Wang, Xiangju, Johnston, Rebecca L., Adams, Benjamin, Grivei, Anca, Ho, Kenneth, Ng, Monica, Waddell, Nicola, Forbes, Josephine M., Healy, Helen and Kassianos, Andrew J. (2023). Hypoxia and interleukin (IL)-1β synergistically induce cellular senescence in human proximal tubular epithelial cells (PTEC). 58th Annual Scientific Meeting of the Australian and New Zealand Society of Nephrology (ANZSN), Christchurch, New Zealand, 4-6 September 2023. Richmond, VIC, Australia: John Wiley & Sons. doi: 10.1111/nep.14216