The University of Queensland

Protocols for measuring the stability and cytotoxicity of cyclotides

Huang, Yen-Hua, Kan, Meng-Wei and Craik, David J. (2021). Protocols for measuring the stability and cytotoxicity of cyclotides. Antimicrobial peptides. (pp. 19-40) edited by Leslie M. Hicks. Cambridge, MA, United States: Academic Press. doi: 10.1016/bs.mie.2021.09.007

Membrane binding of cyclotides

Wang, Conan K., Huang, Yen-Hua, Greenwood, Kathryn and Craik, David J. (2010). Membrane binding of cyclotides. Membrane-active peptides: Methods and results on structure and function. (pp. 597-672) edited by Miguel A.R.B. Castanho. La Jolla, CA, U.S.A.: International University Line.

Molecular dynamics simulations support a preference of cyclotide kalata B1 for phosphatidylethanolamine phospholipids

Roseli, Ras Baizureen, Huang, Yen-Hua, Henriques, Sónia Troeira, Kaas, Quentin and Craik, David J. (2024). Molecular dynamics simulations support a preference of cyclotide kalata B1 for phosphatidylethanolamine phospholipids. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1866 (3) 184268, 184268. doi: 10.1016/j.bbamem.2023.184268

Delivery to, and Reactivation of, the p53 Pathway in Cancer Cells Using a Grafted Cyclotide Conjugated with a Cell-Penetrating Peptide

Philippe, Grégoire Jean-Baptiste, Huang, Yen-Hua, Mittermeier, Anna, Brown, Christopher J., Kaas, Quentin, Ramlan, Siti Radhiah, Wang, Conan K., Lane, David, Loewer, Alexander, Troeira Henriques, Sónia and Craik, David J. (2024). Delivery to, and Reactivation of, the p53 Pathway in Cancer Cells Using a Grafted Cyclotide Conjugated with a Cell-Penetrating Peptide. Journal of Medicinal Chemistry, 67 (2), 1197-1208. doi: 10.1021/acs.jmedchem.3c01682

Scanning mutagenesis identifies residues that improve the long-term stability and insecticidal activity of cyclotide kalata B1

Huang, Yen-Hua, Jiang, Zhihao, Du, Qingdan, Yap, Kuok, Bigot, Aurélien, Kaas, Quentin, Wang, Conan K. and Craik, David J. (2024). Scanning mutagenesis identifies residues that improve the long-term stability and insecticidal activity of cyclotide kalata B1. Journal of Biological Chemistry, 300 (3) 105682, 105682. doi: 10.1016/j.jbc.2024.105682

Computational design of α‐conotoxins to target specific nicotinic acetylcholine receptor subtypes

Wu, Xiaosa, Hone, Arik J, Huang, Yen-Hua, Clark, Richard J, McIntosh, J Michael, Kaas, Quentin and Craik, David J (2023). Computational design of α‐conotoxins to target specific nicotinic acetylcholine receptor subtypes. Chemistry – A European Journal, 30 (7) e202302909, e202302909. doi: 10.1002/chem.202302909

The circular bacteriocin enterocin NKR-5-3B has an improved stability profile over nisin

Wang, Conan K., Huang, Yen-Hua, Shabbir, Fatima, Pham, Huong T., Lawrence, Nicole, Benfield, Aurélie H., van der Donk, Wilfred, Henriques, Sónia T., Turner, Mark S. and Craik, David J. (2023). The circular bacteriocin enterocin NKR-5-3B has an improved stability profile over nisin. Peptides, 167 171049, 171049. doi: 10.1016/j.peptides.2023.171049

Nematicidal activity of cyclotides: toxicity against​​​​​​​ Caenorhabditis elegans

Bajpai, Abhishek, Jackson, Mark A., Huang, Yen-Hua, Yap, Kuok, Du, Qingdan, Chau, Tevin Chui-Ying, Craik, David J. and Gilding, Edward K. (2023). Nematicidal activity of cyclotides: toxicity against​​​​​​​ Caenorhabditis elegans. Journal of Natural Products, 86 (5), 1222-1229. doi: 10.1021/acs.jnatprod.2c01124

Heparan sulfate regulates IL-21 bioavailability and signal strength that control germinal center B cell selection and differentiation

Chen, Zhian, Cui, Yanfang, Yao, Yin, Liu, Bo, Yunis, Joseph, Gao, Xin, Wang, Naiqi, Cañete, Pablo F., Tuong, Zewen Kelvin, Sun, Hongjian, Wang, Hao, Yang, Siling, Wang, Runli, Leong, Yew Ann, Simon Davis, David, Qin, Jiahuan, Liang, Kaili, Deng, Jun, Wang, Conan K., Huang, Yen-Hua, Roco, Jonathan A., Nettelfield, Sam, Zhu, Huaming, Xu, Huajun, Yu, Zhijia, Craik, David, Liu, Zheng, Qi, Hai, Parish, Christopher and Yu, Di (2023). Heparan sulfate regulates IL-21 bioavailability and signal strength that control germinal center B cell selection and differentiation. Science immunology, 8 (80) eadd1728, 1-17. doi: 10.1126/sciimmunol.add1728

Improving stability enhances in vivo efficacy of a PCSK9 inhibitory peptide

Zhang, Yuhui, Wang, Li, Tombling, Benjamin J., Lammi, Carmen, Huang, Yen-Hua, Li, Yue, Bartolomei, Martina, Hong, Bin, Craik, David J. and Wang, Conan K. (2022). Improving stability enhances in vivo efficacy of a PCSK9 inhibitory peptide. Journal of the American Chemical Society, 144 (42), 19485-19498. doi: 10.1021/jacs.2c08029

The acyclotide ribe 31 from Rinorea bengalensis has selective cytotoxicity and potent insecticidal properties in Drosophila

Dang, Tien T., Huang, Yen-Hua, Ott, Stanislav, Harvey, Peta J., Gilding, Edward K., Tombling, Benjamin J., Chan, Lai Y., Kaas, Quentin, Claridge-Chang, Adam and Craik, David J. (2022). The acyclotide ribe 31 from Rinorea bengalensis has selective cytotoxicity and potent insecticidal properties in Drosophila. Journal of Biological Chemistry, 298 (10) 102413, 1-11. doi: 10.1016/j.jbc.2022.102413

Accurate de novo design of membrane-traversing macrocycles

Bhardwaj, Gaurav, O’Connor, Jacob, Rettie, Stephen, Huang, Yen-Hua, Ramelot, Theresa A., Mulligan, Vikram Khipple, Alpkilic, Gizem Gokce, Palmer, Jonathan, Bera, Asim K., Bick, Matthew J., Di Piazza, Maddalena, Li, Xinting, Hosseinzadeh, Parisa, Craven, Timothy W., Tejero, Roberto, Lauko, Anna, Choi, Ryan, Glynn, Calina, Dong, Linlin, Griffin, Robert, van Voorhis, Wesley C., Rodriguez, Jose, Stewart, Lance, Montelione, Gaetano T., Craik, David and Baker, David (2022). Accurate de novo design of membrane-traversing macrocycles. Cell, 185 (19), 3520-3532.e26. doi: 10.1016/j.cell.2022.07.019

Mutagenesis of cyclotide Cter 27 exemplifies a robust folding strategy for bracelet cyclotides

Dang, Tien T., Harvey, Peta J., Chan, Lai Yue, Huang, Yen-Hua, Kaas, Quentin and Craik, David J. (2022). Mutagenesis of cyclotide Cter 27 exemplifies a robust folding strategy for bracelet cyclotides. Peptide Science, 114 (6) e24284. doi: 10.1002/pep2.24284

Mutagenesis of bracelet cyclotide hyen D reveals functionally and structurally critical residues for membrane binding and cytotoxicity

Du, Qingdan, Huang, Yen-Hua, Wang, Conan K., Kaas, Quentin and Craik, David J. (2022). Mutagenesis of bracelet cyclotide hyen D reveals functionally and structurally critical residues for membrane binding and cytotoxicity. Journal of Biological Chemistry, 298 (4) 101822, 101822. doi: 10.1016/j.jbc.2022.101822

Comparative analysis of cyclotide-producing plant cell suspensions presents opportunities for cyclotide plant molecular farming

Doffek, Benjamin, Huang, Yvonne, Huang, Yen-Hua, Chan, Lai Yue, Gilding, Edward K., Jackson, Mark A. and Craik, David J. (2022). Comparative analysis of cyclotide-producing plant cell suspensions presents opportunities for cyclotide plant molecular farming. Phytochemistry, 195 113053, 113053. doi: 10.1016/j.phytochem.2021.113053

Towards a generic prototyping approach for therapeutically-relevant peptides and proteins in a cell-free translation system

Wu, Yue, Cui, Zhenling, Huang, Yen-Hua, de Veer, Simon J., Aralov, Andrey V., Guo, Zhong, Moradi, Shayli V., Hinton, Alexandra O., Deuis, Jennifer R., Guo, Shaodong, Chen, Kai-En, Collins, Brett M., Vetter, Irina, Herzig, Volker, Jones, Alun, Cooper, Matthew A., King, Glenn F., Craik, David J., Alexandrov, Kirill and Mureev, Sergey (2022). Towards a generic prototyping approach for therapeutically-relevant peptides and proteins in a cell-free translation system. Nature Communications, 13 (1) 260, 260. doi: 10.1038/s41467-021-27854-9

ɑO-Conotoxin GeXIVA isomers modulate N-type calcium (CaV2.2) channels and inwardly-rectifying potassium (GIRK) channels via GABAB receptor activation

Yousuf, Arsalan, Wu, Xiaosa, Bony, Anuja R., Sadeghi, Mahsa, Huang, Yen-Hua, Craik, David J. and Adams, David J. (2022). ɑO-Conotoxin GeXIVA isomers modulate N-type calcium (CaV2.2) channels and inwardly-rectifying potassium (GIRK) channels via GABAB receptor activation. Journal of Neurochemistry, 160 (2), 154-171. doi: 10.1111/jnc.15535

Rational design of potent peptide inhibitors of the PD-1:PD-L1 interaction for cancer immunotherapy

Yin, Huawu, Zhou, Xiuman, Huang, Yen-Hua, King, Gordon J., Collins, Brett M., Gao, Yanfeng, Craik, David J. and Wang, Conan K. (2021). Rational design of potent peptide inhibitors of the PD-1:PD-L1 interaction for cancer immunotherapy. Journal of the American Chemical Society, 143 (44) jacs.1c08132, 18536-18547. doi: 10.1021/jacs.1c08132

An ultrapotent and selective cyclic peptide inhibitor of human β-factor XIIa in a cyclotide scaffold

Liu, Wenyu, de Veer, Simon J., Huang, Yen-Hua, Sengoku, Toru, Okada, Chikako, Ogata, Kazuhiro, Zdenek, Christina N., Fry, Bryan G., Swedberg, Joakim E., Passioura, Toby, Craik, David J. and Suga, Hiroaki (2021). An ultrapotent and selective cyclic peptide inhibitor of human β-factor XIIa in a cyclotide scaffold. Journal of the American Chemical Society, 143 (44) jacs.1c07574, 18481-18489. doi: 10.1021/jacs.1c07574

Enabling efficient folding and high-resolution crystallographic analysis of bracelet cyclotides

Huang, Yen-Hua, Du, Qingdan, Jiang, Zhihao, King, Gordon J., Collins, Brett M., Wang, Conan K. and Craik, David J. (2021). Enabling efficient folding and high-resolution crystallographic analysis of bracelet cyclotides. Molecules, 26 (18) 5554. doi: 10.3390/molecules26185554

The emerging landscape of peptide-based inhibitors of PCSK9

Tombling, Benjamin J., Zhang, Yuhui, Huang, Yen-Hua, Craik, David J. and Wang, Conan K. (2021). The emerging landscape of peptide-based inhibitors of PCSK9. Atherosclerosis, 330, 52-60. doi: 10.1016/j.atherosclerosis.2021.06.903

An integrated molecular grafting approach for the design of Keap1-targeted peptide inhibitors

Yin, Huawu, Huang, Yen-Hua, Best, Sarah A., Sutherland, Kate D., Craik, David J. and Wang, Conan K. (2021). An integrated molecular grafting approach for the design of Keap1-targeted peptide inhibitors. ACS Chemical Biology, 16 (7) acschembio.1c00388, 1276-1287. doi: 10.1021/acschembio.1c00388

Effects of backbone cyclization on the pharmacokinetics and drug efficiency of the orally active analgesic conotoxin cVc1.1

Poth, Aaron G., Chiu, Francis C.K., Stalmans, Sofie, Hamilton, Brett R., Huang, Yen-Hua, Shackleford, David M., Patil, Rahul, Le, Thao T., Kan, Meng-Wei, Durek, Thomas, Wynendaele, Evelien, De Spiegeleer, Bart, Powell, Andrew K., Venter, Deon J., Clark, Richard J., Charman, Susan A. and Craik, David J. (2021). Effects of backbone cyclization on the pharmacokinetics and drug efficiency of the orally active analgesic conotoxin cVc1.1. Medicine in Drug Discovery, 10 100087, 1-10. doi: 10.1016/j.medidd.2021.100087

Angler peptides: macrocyclic conjugates inhibit p53:MDM2/X interactions and activate apoptosis in cancer cells

Philippe, Grégoire J.-B., Mittermeier, Anna, Lawrence, Nicole, Huang, Yen-Hua, Condon, Nicholas D., Loewer, Alexander, Craik, David J. and Henriques, Sónia T. (2021). Angler peptides: macrocyclic conjugates inhibit p53:MDM2/X interactions and activate apoptosis in cancer cells. ACS Chemical Biology, 16 (2) acschembio.0c00988, 414-428. doi: 10.1021/acschembio.0c00988

Cyclic gomesin, a stable redesigned spider peptide able to enter cancer cells

Benfield, Aurélie H., Defaus, Sira, Lawrence, Nicole, Chaousis, Stephanie, Condon, Nicholas, Cheneval, Olivier, Huang, Yen-Hua, Chan, Lai Yue, Andreu, David, Craik, David J. and Henriques, Sónia Troeira (2021). Cyclic gomesin, a stable redesigned spider peptide able to enter cancer cells. Biochimica et Biophysica Acta - Biomembranes, 1863 (1) 183480, 183480. doi: 10.1016/j.bbamem.2020.183480

Evaluation of the in vivo aphrodisiac activity of a cyclotide extract from Hybanthus enneaspermus

Du, Qingdan, Huang, Yen-Hua, Bajpai, Abhishek, Frosig- Jorgensen, Majbrit, Zhao, Guangzu and Craik, David J. (2020). Evaluation of the in vivo aphrodisiac activity of a cyclotide extract from Hybanthus enneaspermus. Journal of Natural Products, 83 (12), 3736-3743. doi: 10.1021/acs.jnatprod.0c01045

Exploring the sequence diversity of cyclotides from Vietnamese Viola species

Dang, Tien T., Chan, Lai Y., Huang, Yen-Hua, Nguyen, Linh T. T., Kaas, Quentin, Huynh, Tien and Craik, David J. (2020). Exploring the sequence diversity of cyclotides from Vietnamese Viola species. Journal of Natural Products, 83 (6) acs.jnatprod.9b01218, 1817-1828. doi: 10.1021/acs.jnatprod.9b01218

Discovery and mechanistic studies of cytotoxic cyclotides from the medicinal herb Hybanthus enneaspermus

Du, Qingdan, Chan, Lai Y., Gilding, Edward K., Henriques, Sónia Troeira, Condon, Nicholas D., Ravipati, Anjaneya S., Kaas, Quentin, Huang, Yen-Hua and Craik, David J. (2020). Discovery and mechanistic studies of cytotoxic cyclotides from the medicinal herb Hybanthus enneaspermus. Journal of Biological Chemistry, 295 (32), jbc.RA120.012627-10925. doi: 10.1074/jbc.ra120.012627

Cellular uptake and cytosolic delivery of a cyclic cystine knot scaffold

Yin, Huawu, Huang, Yen-Hua, Deprey, Kirsten, Condon, Nicholas D., Kritzer, Joshua A., Craik, David J. and Wang, Conan K. (2020). Cellular uptake and cytosolic delivery of a cyclic cystine knot scaffold. ACS Chemical Biology, 15 (6), 1650-1661. doi: 10.1021/acschembio.0c00297

Insecticidal diversity of butterfly pea (Clitoria ternatea) accessions

Oguis, Georgianna K., Gilding, Edward K., Huang, Yen-Hua, Poth, Aaron G., Jackson, Mark A. and Craik, David J. (2020). Insecticidal diversity of butterfly pea (Clitoria ternatea) accessions. Industrial Crops and Products, 147 112214, 112214. doi: 10.1016/j.indcrop.2020.112214

Enhanced activity against multi-drug resistant bacteria through coapplication of an analogue of Tachyplesin I and an inhibitor of the QseC/B signaling pathway

Yu, Rilei, Wang, Jiayi, So, Lok-Yan, Harvey, Peta J., Shi, Juan, Liang, Jiazhen, Dou, Qin, Li, Xiao, Yan, Xiayi, Huang, Yen-Hua, Xu, Qingliang, Kaas, Quentin, Chow, Ho-Yin, Wong, Kwok-Yin, Craik, David J, Zhang, Xiaohua, Jiang, Tao and Wang, Yan (2020). Enhanced activity against multi-drug resistant bacteria through coapplication of an analogue of Tachyplesin I and an inhibitor of the QseC/B signaling pathway. Journal of Medicinal Chemistry, 63 (7), 3475-3484. doi: 10.1021/acs.jmedchem.9b01563

Circular permutation of the native enzyme-mediated cyclization position in cyclotides

Smithies, Bronwyn J., Huang, Yen-Hua, Jackson, Mark A., Yap, Kuok, Gilding, Edward K., Harris, Karen S., Anderson, Marilyn A. and Craik, David J. (2020). Circular permutation of the native enzyme-mediated cyclization position in cyclotides. ACS Chemical Biology, 15 (4) acschembio.9b00996, 962-969. doi: 10.1021/acschembio.9b00996

Cyclotides: disulfide-rich peptide toxins in plants

Huang, Yen-Hua, Du, Qingdan and Craik, David J. (2019). Cyclotides: disulfide-rich peptide toxins in plants. Toxicon, 172, 33-44. doi: 10.1016/j.toxicon.2019.10.244

Characterization of tachyplesin peptides and their cyclized analogues to improve antimicrobial and anticancer properties

Vernen, Felicitas, Harvey, Peta J., Dias, Susana A., Veiga, Ana Salomé, Huang, Yen-Hua, Craik, David J., Lawrence, Nicole and Troeira Henriques, Sónia (2019). Characterization of tachyplesin peptides and their cyclized analogues to improve antimicrobial and anticancer properties. International Journal of Molecular Sciences, 20 (17) 4184, 4184. doi: 10.3390/ijms20174184

Cell membrane composition drives selectivity and toxicity of designed cyclic helix-loop-helix peptides with cell penetrating and tumor suppressor properties

Philippe, Grégoire J. -B., Gaspar, Diana, Sheng, Caibin, Huang, Yen-Hua, Benfield, Aurélie H., Condon, Nicholas D., Weidmann, Joachim, Lawrence, Nicole, Löwer, Alexander, Castanho, Miguel A. R. B., Craik, David J. and Troeira Henriques, Sónia (2019). Cell membrane composition drives selectivity and toxicity of designed cyclic helix-loop-helix peptides with cell penetrating and tumor suppressor properties. ACS Chemical Biology, 14 (9), 2071-2087. doi: 10.1021/acschembio.9b00593

Pharmacokinetic characterization of kalata B1 and related therapeutics built on the cyclotide scaffold

Poth, Aaron G., Huang, Yen-Hua, Le, Thao T., Kan, Meng-Wei and Craik, David J. (2019). Pharmacokinetic characterization of kalata B1 and related therapeutics built on the cyclotide scaffold. International Journal of Pharmaceutics, 565, 437-446. doi: 10.1016/j.ijpharm.2019.05.001

Efficient enzymatic cyclization of disulfide-rich peptides by using peptide ligases

Schmidt, Marcel, Huang, Yen-Hua, de Oliveira, Eduardo F. Texeira, Toplak, Ana, Wijma, Hein J., Janssen, Dick B., van Maarseveen, Jan H., Craik, David J. and Nuijens, Timo (2019). Efficient enzymatic cyclization of disulfide-rich peptides by using peptide ligases. ChemBioChem, 20 (12) cbic.201900033, 1524-1529. doi: 10.1002/cbic.201900033

Insecticidal spider toxins are high affinity positive allosteric modulators of the nicotinic acetylcholine receptor

Chambers, Chris, Cutler, Penny, Huang, Yen-Hua, Goodchild, James A., Blythe, Judith, Wang, Conan K., Bigot, Aurélien, Kaas, Quentin, Craik, David J., Sabbadin, Davide and Earley, Fergus G. (2019). Insecticidal spider toxins are high affinity positive allosteric modulators of the nicotinic acetylcholine receptor. FEBS Letters, 593 (12) 1873-3468.13435, 1336-1350. doi: 10.1002/1873-3468.13435

Synthesis, racemic x-ray crystallographic, and permeability studies of bioactive orbitides from Jatropha species

Ramalho, Suelem D., Wang, Conan K., King, Gordon J., Byriel, Karl A., Huang, Yen-Hua, Bolzani, Vanderlan S. and Craik, David J. (2018). Synthesis, racemic x-ray crystallographic, and permeability studies of bioactive orbitides from Jatropha species. Journal of Natural Products, 81 (11) acs.jnatprod.8b00447, 2436-2445. doi: 10.1021/acs.jnatprod.8b00447

Stoichiometry dependent inhibition of rat α3β4 nicotinic acetylcholine receptor by the ribbon isomer of α-conotoxin AuIB

Wu, Xiaosa, Tae, Han-Shen, Huang, Yen-Hua, Adams, David J., Craik, David J. and Kaas, Quentin (2018). Stoichiometry dependent inhibition of rat α3β4 nicotinic acetylcholine receptor by the ribbon isomer of α-conotoxin AuIB. Biochemical Pharmacology, 155, 288-297. doi: 10.1016/j.bcp.2018.07.007

Redesigned spider peptide with improved antimicrobial and anticancer properties

Henriques, Sónia Troeira, Lawrence, Nicole, Chaousis, Stephanie, Ravipati, Anjaneya S., Cheneval, Olivier, Benfield, Aurélie H., Elliott, Alysha G., Kavanagh, Angela Maria, Cooper, Matthew A., Chan, Lai Yue, Huang, Yen-Hua and Craik, David J. (2017). Redesigned spider peptide with improved antimicrobial and anticancer properties. ACS Chemical Biology, 12 (9), 2324-2334. doi: 10.1021/acschembio.7b00459

Backbone cyclization of analgesic conotoxin GeXIVA facilitates direct folding of the ribbon isomer

Wu, Xiaosa, Huang, Yen-Hua, Kaas, Quentin, Harvey, Peta J., Wang, Conan K., Tae, Han-Shen, Adams, David J. and Craik, David J. (2017). Backbone cyclization of analgesic conotoxin GeXIVA facilitates direct folding of the ribbon isomer. Journal of Biological Chemistry, 292 (41), 17101-17112. doi: 10.1074/jbc.M117.808386

Lengths of the C-terminus and interconnecting loops impact stability of spider-derived gating modifier toxins

Agwa, Akello J., Huang, Yen-Hua, Craik, David J., Henriques, Sonia T. and Schroeder, Christina I. (2017). Lengths of the C-terminus and interconnecting loops impact stability of spider-derived gating modifier toxins. Toxins, 9 (8) 248, 248. doi: 10.3390/toxins9080248

Understanding the diversity and distribution of cyclotides from plants of varied genetic origin

Ravipati, Anjaneya S., Poth, Aaron G., Henriques, Sonia Troeira, Bhandari, Murari, Huang, Yen-Hua, Nino, Jaime, Colgrave, Michelle L. and Craik, David J. (2017). Understanding the diversity and distribution of cyclotides from plants of varied genetic origin. Journal of Natural Products, 80 (5), 1522-1530. doi: 10.1021/acs.jnatprod.7b00061

Structural and functional characterization of chimeric cyclotides from the Möbius and trypsin inhibitor subfamilies

Abdul Ghani, Hafiza, Henriques, Sonia Troeira, Huang, Yen-Hua, Swedberg, Joakim E. , Schroeder, Christina I. and Craik, David J. (2017). Structural and functional characterization of chimeric cyclotides from the Möbius and trypsin inhibitor subfamilies. Biopolymers, 108 (1) e22927, e22927. doi: 10.1002/bip.22927

Cyclisation of disulfide-rich conotoxins in drug design applications

Wu, Xiaosa, Huang, Yen-Hua, Kaas, Quentin and Craik, David J. (2016). Cyclisation of disulfide-rich conotoxins in drug design applications. European Journal of Organic Chemistry, 2016 (21), 3462-3472. doi: 10.1002/ejoc.201600402

Front Cover: Cyclisation of Disulfide-Rich Conotoxins in Drug Design Applications (Eur. J. Org. Chem. 21/2016)

Wu, Xiaosa, Huang, Yen-Hua, Kaas, Quentin and Craik, David J. (2016). Front Cover: Cyclisation of Disulfide-Rich Conotoxins in Drug Design Applications (Eur. J. Org. Chem. 21/2016). European Journal of Organic Chemistry, 2016 (21), 3457-3457. doi: 10.1002/ejoc.201670211

Inhibition of tau aggregation using a naturally-occurring cyclic peptide scaffold

Wang, Conan K., Northfield, Susan E., Huang, Yen-Hua, Ramos, Mariana C. and Craik, David J. (2016). Inhibition of tau aggregation using a naturally-occurring cyclic peptide scaffold. European Journal of Medicinal Chemistry, 109, 342-349. doi: 10.1016/j.ejmech.2016.01.006

Development of cell-penetrating peptide-based drug leads to inhibit MDMX:p53 and MDM2:p53 interactions

Philippe, Gregoire, Huang, Yen-Hua, Cheneval, Olivier, Lawrence, Nicole, Zhang, Zhen, Fairlie, David P., Craik, David J., Dantas De Araujo, Aline and Troeira Henriques, Sonia (2016). Development of cell-penetrating peptide-based drug leads to inhibit MDMX:p53 and MDM2:p53 interactions. Biopolymers - Peptide Science, 106 (6), 853-863. doi: 10.1002/bip.22893

The Prototypic Cyclotide Kalata B1 Has a Unique Mechanism of Entering Cells

Henriques, Sonia Troeira, Huang, Yen-Hua, Chaousis, Stephanie, Sani, Marc-Antoine, Poth, Aaron G., Separovic, Frances and Craik, David J. (2015). The Prototypic Cyclotide Kalata B1 Has a Unique Mechanism of Entering Cells. Chemistry and Biology, 22 (8) 3107, 1087-1097. doi: 10.1016/j.chembiol.2015.07.012

Design of substrate-based BCR-ABL kinase inhibitors using the cyclotide scaffold

Huang, Yen-Hua, Henriques, Sonia T., Wang, Conan K., Thorstholm, Louise, Daly, Norelle L., Kaas, Quentin and Craik, David J. (2015). Design of substrate-based BCR-ABL kinase inhibitors using the cyclotide scaffold. Scientific Reports, 5 (1) 12974, 1-15. doi: 10.1038/srep12974

Optimization of the cyclotide framework to improve cell penetration properties

Huang, Yen-Hua, Chaousis, Steph, Cheneval, Olivier, Craik, David J. and Henriques, Sonia Troeira (2015). Optimization of the cyclotide framework to improve cell penetration properties. Frontiers in Pharmacology, 6 (17) 17, 1-7. doi: 10.3389/fphar.2015.00017

Anticancer and toxic properties of cyclotides are dependent on phosphatidylethanolamine phospholipid targeting

Henriques, Sonia Troeira, Huang, Yen-Hua, Chaousis, Steph, Wang, Conan and Craik, David J. (2014). Anticancer and toxic properties of cyclotides are dependent on phosphatidylethanolamine phospholipid targeting. Chembiochem, 15 (13), 1956-1965. doi: 10.1002/cbic.201402144

Effects of arginine 10 to lysine substitution on omega-conotoxin CVIE and CVIF block of Ca(v)2.2 channels

Berecki, G., Daly, N. L., Huang, Y. H., Vink, S., Craik, D. J., Alewood, P. F. and Adams, D. J. (2014). Effects of arginine 10 to lysine substitution on omega-conotoxin CVIE and CVIF block of Ca(v)2.2 channels. British Journal of Pharmacology, 171 (13), 3313-3327. doi: 10.1111/bph.12686

Fmoc-based synthesis of disulfide-rich cyclic peptides

Cheneval, Olivier, Schroeder, Christina I., Durek, Thomas, Walsh, Phillip, Huang, Yen-Hua, Liras, Spiros, Price, David A. and Craik, David J. (2014). Fmoc-based synthesis of disulfide-rich cyclic peptides. Journal of Organic Chemistry, 79 (12), 5538-5544. doi: 10.1021/jo500699m

Semienzymatic cyclization of disulfide-rich peptides using sortase A

Jia, Xinying, Kwon, Soohyun, Wang, Ching-I Anderson, Huang, Yen-Hua, Chan, Lai Y., Tan, Chia Chia, Rosengren, K. Johan, Mulvenna, Jason P., Schroeder, Christina I. and Craik, David J. (2014). Semienzymatic cyclization of disulfide-rich peptides using sortase A. Journal of Biological Chemistry, Papers in Press (10), 1-25. doi: 10.1074/jbc.M113.539262

A novel quantitative kinase assay using bacterial surface display and flow cytometry

Henriques, Sónia Troeira, Thorstholm, Louise, Huang, Yen-Hua, Getz, Jennifer A., Daugherty, Patrick S. and Craik, David J. (2013). A novel quantitative kinase assay using bacterial surface display and flow cytometry. PloS One, 8 (11) e80474, e80474.1-e80474.7. doi: 10.1371/journal.pone.0080474

The Antimicrobial Activity of Sub3 is Dependent on Membrane Binding and Cell-Penetrating Ability

Torcato, Ines M., Huang, Yen-Hua, Franquelim, Henri G., Gaspar, Diana D., Craik, David J., Castanho, Miguel A. R. B. and Henriques, Sonia Troeira (2013). The Antimicrobial Activity of Sub3 is Dependent on Membrane Binding and Cell-Penetrating Ability. Chembiochem, 14 (15), 2013-2022. doi: 10.1002/cbic.201300274

Cyclotides suppress human T-Lymphocyte proliferation by an interleukin 2-dependent mechanism

Gruendemann, Carsten, Thell, Kathrin, Lengen, Karin, Garcia-Kaeufer, Manuel, Huang, Yen-Hua, Huber, Roman, Craik, David J., Schabbauer, Gernot and Gruber, Christian W. (2013). Cyclotides suppress human T-Lymphocyte proliferation by an interleukin 2-dependent mechanism. Plos One, 8 (6) e68016, e68016.1-e68016.12. doi: 10.1371/journal.pone.0068016

High-affinity cyclic peptide matriptase inhibitors

Quimbar, Pedro, Malik, Uru, Sommerhoff, Christian P., Kaas, Quentin, Chan, Lai Y., Huang, Yen-Hua, Grundhuber, Maresa, Dunse, Kerry, Craik, David J., Anderson, Marilyn A. and Daly, Norelle L. (2013). High-affinity cyclic peptide matriptase inhibitors. Journal of Biological Chemistry, 288 (19), 13885-13896. doi: 10.1074/jbc.M113.460030

Cyclization of the antimicrobial peptide gomesin with native chemical ligation: influences on stability and bioactivity

Chan, Lai Yue, Zhang, Veronica M., Huang, Yen-hua, Waters, Norman C., Bansal, Paramjit S., Craik, David J. and Daly, Norelle L. (2013). Cyclization of the antimicrobial peptide gomesin with native chemical ligation: influences on stability and bioactivity. Chembiochem, 14 (5), 617-624. doi: 10.1002/cbic.201300034

Design and characterization of novel antimicrobial peptides, R-BP100 and RW-BP100, with activity against Gram-negative and Gram-positive bacteria

Torcato, Inês M., Huang, Yen-Hua, Franquelim, Henri G., Gaspar, Diana, Craik, David J., Castanho, Miguel A. R. B. and Henriques, Sonia Troeira (2013). Design and characterization of novel antimicrobial peptides, R-BP100 and RW-BP100, with activity against Gram-negative and Gram-positive bacteria. Biochimica et Biophysica Acta, 1828 (3), 944-955. doi: 10.1016/j.bbamem.2012.12.002

Phosphatidylethanolamine-binding is a conserved feature of cyclotide-membrane interactions

Henriques, Sonia Troeira, Huang, Yen-Hua, Castanho, Miguel A. R. B., Bagatolli, Luis A., Sonza, Secondo, Tachedjan, Gilda, Daly, Norelle L. and Craik, David J. (2012). Phosphatidylethanolamine-binding is a conserved feature of cyclotide-membrane interactions. Journal of Biological Chemistry, 287 (40), 33629-33643. doi: 10.1074/jbc.M112.372011

Decoding the membrane activity of the cyclotide kalata B1: The importance of phosphatidylethanolamine phospholipids and lipid organization on hemolytic and anti-HIV activities

Henriques, Sónia Troeira, Huang, Yen-Hua, Rosengren, K. Johan, Franquelim, Henri G., Carvalho, Filomena A., Johnson, Adam, Sonza, Secondo, Tachedjian, Gilda, Castanho, Miguel A. R. B., Daly, Norelle L. and Craik, David J. (2011). Decoding the membrane activity of the cyclotide kalata B1: The importance of phosphatidylethanolamine phospholipids and lipid organization on hemolytic and anti-HIV activities. Journal of Biological Chemistry, 286 (27), 24231-24241. doi: 10.1074/jbc.M111.253393

Identification and characterization of a new family of cell penetrating peptides: Cyclic cell penetrating peptides

Cascales, Laura, Henriques, Sónia T., Kerr, Markus C., Huang, Yen-Hua, Sweet, Matthew J., Daly, Norelle L. and Craik, David J. (2011). Identification and characterization of a new family of cell penetrating peptides: Cyclic cell penetrating peptides. Journal of Biological Chemistry, Epub ahead of print (42), 36932-36943. doi: 10.1074/jbc.M111.264424

Cyclotide interactions with the nematode external surface

Colgrave, Michelle L., Huang, Yen-Hua, Craik, David J. and Kotze, Andrew C. (2010). Cyclotide interactions with the nematode external surface. Antimicrobial Agents and Chemotherapy, 54 (5), 2160-2166. doi: 10.1128/AAC.01306-09

Lysine-scanning mutagenesis reveals an amendable face of the cyclotide kalata B1 for the optimization of nematocidal activity

Huang, Yen-Hua (黃彥華), Colgrave, Michelle L., Clark, Richard J., Kotze, Andrew C. and Craik, David J. (2010). Lysine-scanning mutagenesis reveals an amendable face of the cyclotide kalata B1 for the optimization of nematocidal activity. Journal of Biological Chemistry, 285 (14), 10797-10805. doi: 10.1074/jbc.M109.089854

The Biological Activity of the Prototypic Cyclotide Kalata B1 Is Modulated by the Formation of Multimeric Pores

Huang, Y. H., Colgrave, M. L., Daly, N. L., Keleshian, A, Martinac, B and Craik, D. J. (2009). The Biological Activity of the Prototypic Cyclotide Kalata B1 Is Modulated by the Formation of Multimeric Pores. Journal of Biological Chemistry, 284 (31), 20699-20707. doi: 10.1074/jbc.M109.003384

Cyclotides: Natural, Circular Plant Peptides that possess significant activity against Gastrointestinal nematode parasites of sheep

Colgrave, M. L., Kotze, A. C., Huang, Y., O'Grady, J., Simonsen, S. M. and Craik, D. J. (2008). Cyclotides: Natural, Circular Plant Peptides that possess significant activity against Gastrointestinal nematode parasites of sheep. Biochemistry, 47 (20), 5581-5589. doi: 10.1021/bi800223y

Spider toxins as novel allosteric modulators of insect nicotinic receptors

Earley, Fergus, Chambers, Chris, Cutler, Penny, Huang, Yen-Hua and Craik, David (2019). Spider toxins as novel allosteric modulators of insect nicotinic receptors. ACS Fall National Meeting and Exposition, San Diego, CA, United States, 25-29 August, 2019. Washington, DC United States: American Chemical Society.

Inhibition of nicotinic acetylcholine receptor subtypes by the ribbon isomers of alpha-conotoxins

Kaas, Quentin, Wu, Xiaosa, Tae, Han-Shen, Huang, Yen-Hua, Adams, David J. and Craik, David (2018). Inhibition of nicotinic acetylcholine receptor subtypes by the ribbon isomers of alpha-conotoxins. 35th European Peptide Symposium, Dublin City University, Ireland, 26-31 August 2018. Oxford, United Kingdom: John Wiley & Sons.

Targeting the coiled-coil domain of BCR-ABL1 kinase with new weapons

Huang, Yen-Hua, Passioura, Toby, Peacock, Hayden, Henriques, Sonia Troeira, Wang, Conan, Kaas, Quentin, Suga, Hiroaki and Craik, David (2018). Targeting the coiled-coil domain of BCR-ABL1 kinase with new weapons. 35th European Peptide Symposium, Dublin City University, Ireland, 26-31 August 2018. Oxford, United Kingdom: John Wiley & Sons.

Cyclotides, stable drug scaffolds and delivery systems to target intracellular cancer pathways

Henriques, S. Troeira, Huang, Y. -H. and Craik, D. (2016). Cyclotides, stable drug scaffolds and delivery systems to target intracellular cancer pathways. 34th European Peptide Symposium, Leipzig, Germany, 4-9 September 2016. Oxford, United Kingdom: John Wiley & Sons.

Inhibitory Effects of Substrate-Based Bcr-Abl1 Kinase Inhibitors On the Growth of Chronic Myeloid Leukemia Cell Line K562

Huang, Y. -H., Henriques, S. Troeira, Lawrence, N., Kaas, Q. and Craik, D. J. (2016). Inhibitory Effects of Substrate-Based Bcr-Abl1 Kinase Inhibitors On the Growth of Chronic Myeloid Leukemia Cell Line K562. 34th European Peptide Symposium, Leipzig, Germany, 4‐9 September 2016. Oxford, United Kingdom: John Wiley & Sons. doi: 10.1002/psc.2950

Cyclotides, stable drug scaffolds that enter cells after binding to PE-phospholipids

Henriques, S. T., Huang, Y. -H., Chaouis, S., Sani, M. -A., Poth, A. G., Separovic, F. and Craik, D. J. (2015). Cyclotides, stable drug scaffolds that enter cells after binding to PE-phospholipids. 10th European Biophysical Societies Association (EBSA) European Biophysics Congress, Dresden, Germany, Jul 18-22, 2015. Heidelberg, Germany: Springer.

Cyclotides use specific lipids as a switch to internalize inside cells

Henriques, S. T., Huang, Y-H., Chaousis, S. and Craik, D. J. (2014). Cyclotides use specific lipids as a switch to internalize inside cells. IUPAB Congress 2014, Brisbane, QLD, Australia, 3-7 August 2014. Chichester, West Sussex, United Kingdom: John Wiley & Sons. doi: 10.1002/psc.2687

The Importance of the cell membrane on the mechanism of action of cyclotides

Henriques, S. T., Huang, Y. -H., Castanho, M. A. R. B., Daly, N. L. and Craik, D. J. (2012). The Importance of the cell membrane on the mechanism of action of cyclotides. 32nd European Peptide Symposium "Peptides 2012", Athens, Greece, 02-07 September 2012. West Sussex, United Kingdom: John Wiley and Sons. doi: 10.1002/psc.2449

Differences in bactericidal, hemolytic and anti-HIV efficiencies of the cyclotide kalata B1 are explained by distinct cell membrane compositions

Henriques, Sonia Troeira, Huang, Yen-Boa, Rosengren, K. Johan, Franquelim, Henri G., Carvalho, Filomena A., Johnson, Adam, Sonza, Second, Tachedjian, Gilda, Castanho, Miguel A. R. B., Daly, Norelle L. and Craik, I. David J. (2011). Differences in bactericidal, hemolytic and anti-HIV efficiencies of the cyclotide kalata B1 are explained by distinct cell membrane compositions. 22nd American Peptide Symposium, San Diego, California, 25-30 June 2011 . Hoboken, NJ, United States: John Wiley & Sons.

How Do Cyclotides Exert Their Mode of Action? the Importance of Cell Membranes On Their Diverse Activities

Henriques, S., Huang, Y., Daly, N. and Craik, D. (2009). How Do Cyclotides Exert Their Mode of Action? the Importance of Cell Membranes On Their Diverse Activities. 21st American Peptide Symposium, Bloomington, Indiana, USA, 7-12 June 2009. United States: John Wiley & Sons, Inc.. doi: 10.1002/bip.21223

How do cyclotides exert their mode of action? the importance of cell membranes on their diverse activities

Henriques, S., Huang, Y., Daly, N. and Craik, D. (2009). How do cyclotides exert their mode of action? the importance of cell membranes on their diverse activities. 21st American Peptide Symposium, Bloomington, Indiana, USA, 7-12 June 2009. United States: John Wiley & Sons, Inc.. doi: 10.1002/bip.21222

A combined chemistry and biology approach to the development of novel agents for protection against livestock pests

Huang, Yen-Hua, Colgrave, Michelle L., Kotze, Andrew C. and Craik, David J. (2008). A combined chemistry and biology approach to the development of novel agents for protection against livestock pests. 30th European Peptide Symposium (30EPS), Helsinki, Finland, 31 August – 5 September 2008. United Kingdom: John Wiley & Sons Ltd.. doi: 10.1002/psc.1090

The mode of action of cyclotides: Functional studies of the prototypic cyclotide kalata B1

Yen-Hua Huang (2010). The mode of action of cyclotides: Functional studies of the prototypic cyclotide kalata B1. PhD Thesis, Institute for Molecular Bioscience, The University of Queensland.