The University of Queensland

Flow cytometric reporter assays provide robust functional analysis of signaling complexes

Muusse, Timothy W., Lee, Morris Y.L., Kim, Hyoyoung, Parat, Marie-Odile, Nanson, Jeffrey D., Kobe, Bostjan, Vajjhala, Parimala R. and Stacey, Katryn J. (2022). Flow cytometric reporter assays provide robust functional analysis of signaling complexes. Journal of Biological Chemistry, 298 (12) 102666, 102666. doi: 10.1016/j.jbc.2022.102666

Cyclic ADP ribose isomers: Production, chemical structures, and immune signaling

Manik, Mohammad K., Shi, Yun, Li, Sulin, Zaydman, Mark A., Damaraju, Neha, Eastman, Samuel, Smith, Thomas G., Gu, Weixi, Masic, Veronika, Mosaiab, Tamim, Weagley, James S., Hancock, Steven J., Vasquez, Eduardo, Hartley-Tassell, Lauren, Kargios, Nestoras, Maruta, Natsumi, Lim, Bryan Y. J., Burdett, Hayden, Landsberg, Michael J., Schembri, Mark A., Prokes, Ivan, Song, Lijiang, Grant, Murray, DiAntonio, Aaron, Nanson, Jeffrey D., Guo, Ming, Milbrandt, Jeffrey, Ve, Thomas and Kobe, Bostjan (2022). Cyclic ADP ribose isomers: Production, chemical structures, and immune signaling. Science, 377 (6614) eadc8969, eadc8969-+. doi: 10.1126/science.adc8969

Structural basis of SARM1 activation, substrate recognition, and inhibition by small molecules

Shi, Yun, Kerry, Philip S., Nanson, Jeffrey D., Bosanac, Todd, Sasaki, Yo, Krauss, Raul, Saikot, Forhad K., Adams, Sarah E., Mosaiab, Tamim, Masic, Veronika, Mao, Xianrong, Rose, Faith, Vasquez, Eduardo, Furrer, Marieke, Cunnea, Katie, Brearley, Andrew, Gu, Weixi, Luo, Zhenyao, Brillault, Lou, Landsberg, Michael J., DiAntonio, Aaron, Kobe, Bostjan, Milbrandt, Jeffrey, Hughes, Robert O. and Ve, Thomas (2022). Structural basis of SARM1 activation, substrate recognition, and inhibition by small molecules. Molecular Cell, 82 (9), 1643-1659.e10. doi: 10.1016/j.molcel.2022.03.007

The transmembrane adaptor SCIMP recruits tyrosine kinase Syk to phosphorylate Toll-like receptors to mediate selective inflammatory outputs

Liu, Liping, Lucas, Richard M., Nanson, Jeffrey D., Li, Yan, Whitfield, Jason, Curson, James E. B., Tuladhar, Neeraj, Alexandrov, Kirill, Mobli, Mehdi, Sweet, Matthew J., Kobe, Bostjan, Stow, Jennifer and Luo, Lin (2022). The transmembrane adaptor SCIMP recruits tyrosine kinase Syk to phosphorylate Toll-like receptors to mediate selective inflammatory outputs. Journal of Biological Chemistry, 298 (5) 101857, 101857. doi: 10.1016/j.jbc.2022.101857

Crystal structure of the Toll/interleukin‐1 receptor (TIR) domain of IL‐1R10 provides structural insights into TIR domain signaling

Nimma, Surekha, Gu, Weixi, Manik, Mohammad K., Ve, Thomas, Nanson, Jeffrey D. and Kobe, Bostjan (2022). Crystal structure of the Toll/interleukin‐1 receptor (TIR) domain of IL‐1R10 provides structural insights into TIR domain signaling. FEBS Letters, 596 (7), 886-897. doi: 10.1002/1873-3468.14288

Structural evolution of TIR-domain signalosomes

Nimma, Surekha, Gu, Weixi, Maruta, Natsumi, Li, Yan, Pan, Mengqi, Saikot, Forhad Karim, Lim, Bryan Y. J., McGuinness, Helen Ying, Zaoti, Zannati Ferdous, Li, Sulin, Desa, Sneha, Manik, Mohammad Kawsar, Nanson, Jeffrey D. and Kobe, Bostjan (2021). Structural evolution of TIR-domain signalosomes. Frontiers in Immunology, 12 784484, 1-10. doi: 10.3389/fimmu.2021.784484

Crystal structure determination of the armadillo repeat domain of Drosophila SARM1 using MIRAS phasing

Gu, Weixi, Luo, Zhenyao, Vonrhein, Clemens, Jia, Xinying, Ve, Thomas, Nanson, Jeffrey D. and Kobe, Bostjan (2021). Crystal structure determination of the armadillo repeat domain of Drosophila SARM1 using MIRAS phasing. Acta Crystallographica. Section F: Structural Biology Communications, 77 (10), 364-373. doi: 10.1107/s2053230x21006786

MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography

Clabbers, Max T. B., Holmes, Susannah, Muusse, Timothy W., Vajjhala, Parimala R., Thygesen, Sara J., Malde, Alpeshkumar K., Hunter, Dominic J. B., Croll, Tristan I., Flueckiger, Leonie, Nanson, Jeffrey D., Rahaman, Md. Habibur, Aquila, Andrew, Hunter, Mark S., Liang, Mengning, Yoon, Chun Hong, Zhao, Jingjing, Zatsepin, Nadia A., Abbey, Brian, Sierecki, Emma, Gambin, Yann, Stacey, Katryn J., Darmanin, Connie, Kobe, Bostjan, Xu, Hongyi and Ve, Thomas (2021). MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography. Nature Communications, 12 (1) 2578, 1-14. doi: 10.1038/s41467-021-22590-6

SARM1 is a metabolic sensor activated by an increased NMN/NAD+ ratio to trigger axon degeneration

Figley, Matthew D., Gu, Weixi, Nanson, Jeffrey D., Shi, Yun, Sasaki, Yo, Cunnea, Katie, Malde, Alpeshkumar K., Jia, Xinying, Luo, Zhenyao, Saikot, Forhad K., Mosaiab, Tamim, Masic, Veronika, Holt, Stephanie, Hartley-Tassell, Lauren, McGuinness, Helen Y., Manik, Mohammad K., Bosanac, Todd, Landsberg, Michael J., Kerry, Philip S., Mobli, Mehdi, Hughes, Robert O., Milbrandt, Jeffrey, Kobe, Bostjan, DiAntonio, Aaron and Ve, Thomas (2021). SARM1 is a metabolic sensor activated by an increased NMN/NAD+ ratio to trigger axon degeneration. Neuron, 109 (7), 1-19. doi: 10.1016/j.neuron.2021.02.009

Structural basis for nuclear import selectivity of pioneer transcription factor SOX2

Jagga, Bikshapathi, Edwards, Megan, Pagin, Miriam, Wagstaff, Kylie M., Aragão, David, Roman, Noelia, Nanson, Jeffrey D., Raidal, Shane R., Dominado, Nicole, Stewart, Murray, Jans, David A., Hime, Gary R., Nicolis, Silvia K., Basler, Christopher F. and Forwood, Jade K. (2021). Structural basis for nuclear import selectivity of pioneer transcription factor SOX2. Nature Communications, 12 (1) 28, 1-11. doi: 10.1038/s41467-020-20194-0

Structure, function, and regulation of thioesterases

Swarbrick, Crystall M.D., Nanson, Jeffrey D., Patterson, Edward I. and Forwood, Jade K. (2020). Structure, function, and regulation of thioesterases. Progress in Lipid Research, 79 101036, 101036. doi: 10.1016/j.plipres.2020.101036

Structural characterisation of β-Ketoacyl ACP synthase I bound to platencin and fragment screening molecules at two substrate binding sites

Patterson, Edward I., Nanson, Jeffrey D., Abendroth, Jan, Bryan, Cassie, Sankaran, Banumathi, Myler, Peter J. and Forwood, Jade K. (2020). Structural characterisation of β-Ketoacyl ACP synthase I bound to platencin and fragment screening molecules at two substrate binding sites. Proteins: Structure, Function, and Bioinformatics, 88 (1) prot.25765, 47-56. doi: 10.1002/prot.25765

Structural characterization of a short-chain dehydrogenase/reductase from multi-drug resistant Acinetobacter baumannii

Cross, Emily M., Aragão, David, Smith, Kate M., Shaw, Karli I., Nanson, Jeffrey D., Raidal, Shane R. and Forwood, Jade K. (2019). Structural characterization of a short-chain dehydrogenase/reductase from multi-drug resistant Acinetobacter baumannii. Biochemical and Biophysical Research Communications, 518 (3), 465-471. doi: 10.1016/j.bbrc.2019.08.056

NAD+ cleavage activity by animal and plant TIR domains in cell death pathways

Horsefield, Shane, Burdett, Hayden, Zhang, Xiaoxiao, Manik, Mohammad K., Shi, Yun, Chen, Jian, Qi, Tiancong, Gilley, Jonathan, Lai, Jhih-Siang, Rank, Maxwell X., Casey, Lachlan W., Gu, Weixi, Ericsson, Daniel J., Foley, Gabriel, Hughes, Robert O., Bosanac, Todd, von Itzstein, Mark, Rathjen, John P., Nanson, Jeffrey D., Boden, Mikael, Dry, Ian B., Williams, Simon J., Staskawicz, Brian J., Coleman, Michael P., Ve, Thomas, Dodds, Peter N. and Kobe, Bostjan (2019). NAD+ cleavage activity by animal and plant TIR domains in cell death pathways. Science, 365 (6455), 793-799. doi: 10.1126/science.aax1911

Death, TIR, and RHIM: Self-assembling domains involved in innate immunity and cell-death signaling

Nanson, Jeffrey D., Kobe, Bostjan and Ve, Thomas (2019). Death, TIR, and RHIM: Self-assembling domains involved in innate immunity and cell-death signaling. Journal of Leukocyte Biology, 105 (2), 363-375. doi: 10.1002/JLB.MR0318-123R

Regulation of signaling by cooperative assembly formation in mammalian innate immunity signalosomes by molecular mimics

Nanson, Jeffrey D., Rahaman, Habibur, Ve, Thomas and Kobe, Bostjan (2018). Regulation of signaling by cooperative assembly formation in mammalian innate immunity signalosomes by molecular mimics. Seminars in Cell and Developmental Biology, 99, 96-114. doi: 10.1016/j.semcdb.2018.05.002

Structural characterisation of the fatty acid biosynthesis enzyme FabF from the pathogen Listeria monocytogenes

Soares Da Costa, Tatiana P., Nanson, Jeffrey D. and Forwood, Jade K. (2017). Structural characterisation of the fatty acid biosynthesis enzyme FabF from the pathogen Listeria monocytogenes. Scientific Reports, 7 (1) 39277, 39277. doi: 10.1038/srep39277

Structural characterisation of FabG from yersinia pestis, a key component of bacterial fatty acid synthesis

Nanson, Jeffrey D. and Forwood, Jade K. (2015). Structural characterisation of FabG from yersinia pestis, a key component of bacterial fatty acid synthesis. PloS One, 10 (11) e0141543, e0141543. doi: 10.1371/journal.pone.0141543

Structural characterisation of the Beta-Ketoacyl-Acyl carrier protein synthases, FabF and FabH, of Yersinia pestis

Nanson, Jeffrey D., Himiari, Zainab, Swarbrick, Crystall M. D. and Forwood, Jade K. (2015). Structural characterisation of the Beta-Ketoacyl-Acyl carrier protein synthases, FabF and FabH, of Yersinia pestis. Scientific Reports, 5 (1) 14797. doi: 10.1038/srep14797

Structural basis for regulation of the human acetyl-CoA thioesterase 12 and interactions with the steroidogenic acute regulatory protein-related lipid transfer (START) domain

Swarbrick, Crystall M. D., Roman, Noelia, Cowieson, Nathan, Patterson, Edward I., Nanson, Jeffrey, Siponen, Marina I., Berglund, Helena, Lehtio, Lari and Forwood, Jade K. (2014). Structural basis for regulation of the human acetyl-CoA thioesterase 12 and interactions with the steroidogenic acute regulatory protein-related lipid transfer (START) domain. Journal of Biological Chemistry, 289 (35), 24263-24274. doi: 10.1074/jbc.M114.589408

Crystallization and preliminary X-ray diffraction analysis of FabG from Yersinia pestis

Nanson, Jeffrey David and Forwood, Jade Kenneth (2014). Crystallization and preliminary X-ray diffraction analysis of FabG from Yersinia pestis. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 70 (1), 101-104. doi: 10.1107/S2053230X13033402