Prof Tim Mahony joined QAAFI’s Centre for Animal Science in October 2010, after 15 years of conducting research projects with the Queensland Government. He obtained his PhD from James Cook University in the area of molecular microbiology. During 2001 and 2002, Prof Mahony was a visiting professor at the University of Saskatchewan.
Research interests
Dr Mahony’s research interests are in the area of molecular virology revolving around improving viral disease control in production animals such as cattle and poultry. His group is characterising the molecular interactions between invading pathogens and the subsequent host responses with the goal of developing new vaccines and diagnostic technologies. A key component of this work includes improving the basic understanding of the molecular mechanisms that underpin and drive viral virulence and evolution. Next-generation sequencing has been used to sequence the genomes of herpesviruses and adenoviruses from a variety of species including, cattle, chickens, marsupials, horses, and crocodiles. Prof Mahony’s team is also investigating the role of virally encoded microRNAs in virulence, replication, and disease development. His group was one of the first in the world to apply bacterial artificial chromosome infectious clone technology for the efficient manipulation of herpesviruses that have large DNA genomes. These strategies are also being exploited to understand viral gene function and the development of vaccines.
Currently, Prof Mahony is researching risk factors that protect and predispose feedlot cattle to developing bovine respiratory disease (BRD). He is also leading the development of new vaccines for BRD and cattle tick infestations. Prof Mahony has also led the development of new vaccine delivery technologies for the poultry industry targeting the application of in ovo strategies. Prof Mahony research into improving animal health is increasing industry productivity, food safety, and consumer confidence in food products and he is keen to explore collaborations with other scientists in this area.
Prof Mahony has a strong interest to work with post-graduate students and has supervised a number of post-graduate students from Australia and overseas. These projects have covered research areas in molecular virology, animal health, vaccine development, and pathogen-host interactions.
Journal Article: Skim-Nanopore sequencing for routine genomic evaluation and bacterial pathogen detection in cattle
Lamb, H. J., Nguyen, L. T., Briody, T. E., Ambrose, R. K., Hayes, B. J., Mahony, T. J. and Ross, E. M. (2023). Skim-Nanopore sequencing for routine genomic evaluation and bacterial pathogen detection in cattle. Animal Production Science, 63 (11), 1074-1085. doi: 10.1071/an22451
Journal Article: Statement in Support of: “Virology under the Microscope—a Call for Rational Discourse”
Speck, Peter, Mackenzie, Jason, Bull, Rowena A., Slobedman, Barry, Drummer, Heidi, Fraser, Johanna, Herrero, Lara, Helbig, Karla, Londrigan, Sarah, Moseley, Gregory, Prow, Natalie, Hansman, Grant, Edwards, Robert, Ahlenstiel, Chantelle, Abendroth, Allison, Tscharke, David, Hobson-Peters, Jody, Kriiger-Loterio, Robson, Parry, Rhys, Marsh, Glenn, Harding, Emma, Jacques, David A., Gartner, Matthew J., Lee, Wen Shi, McAuley, Julie, Vaz, Paola, Sainsbury, Frank, Tate, Michelle D., Sinclair, Jane ... Young, Paul (2023). Statement in Support of: “Virology under the Microscope—a Call for Rational Discourse”. Journal of Virology, 97 (5), e0045123. doi: 10.1128/jvi.00451-23
Journal Article: Statement in Support of: “Virology under the Microscope—a Call for Rational Discourse”
Speck, Peter, Mackenzie, Jason, Bull, Rowena A., Slobedman, Barry, Drummer, Heidi, Fraser, Johanna, Herrero, Lara, Helbig, Karla, Londrigan, Sarah, Moseley, Gregory, Prow, Natalie, Hansman, Grant, Edwards, Robert, Ahlenstiel, Chantelle, Abendroth, Allison, Tscharke, David, Hobson-Peters, Jody, Kriiger-Loterio, Robson, Parry, Rhys, Marsh, Glenn, Harding, Emma, Jacques, David A., Gartner, Matthew J., Lee, Wen Shi, McAuley, Julie, Vaz, Paola, Sainsbury, Frank, Tate, Michelle D., Sinclair, Jane ... Young, Paul (2023). Statement in Support of: “Virology under the Microscope—a Call for Rational Discourse”. mBio, 14 (3), e0081523. doi: 10.1128/mbio.00815-23
Evaluation of a prototype vaccine for rattles in foals
(2023–2025) Agrifutures Australia
Saving the endangered Spectacled flying fox from annual tick paralysis mortality events:
(2023–2025) Threatened Species Research Grants
Harnessing the synbio potential of Australia's stingless bees, the first step
(2023–2024) Sugar Research Australia Limited
The Development of a Novel Approach for the Differentiation of Bovine herpesvirus 1 field and vaccine strains
Master Philosophy
The application of genome editing to improve the disease resilience of livestock species
Doctor Philosophy
Identification of the host and viral transcripts that are post-transcriptionally regulated by the microRNAs encoded by bovine herpesvirus 1
Doctor Philosophy
The development of strand-specific sequencing methodologies double-stranded DNA viral genomes
The herpesviruses are a large family of viruses that infected a broad range of hosts, such as mammals, reptiles, and molluscs. These viruses have a large double stranded DNA (dsDNA) genome. While the herpesviruses are considered to be genetically stable, variants with increased capacity to cause disease have emerged. The molecular basis underpinning the emergence of these virulent subtypes/genotypes for several herpesviruses of importance to veterinary medicine remains poorly understood. This project will utilise Oxford Nanopore sequencing technology to develop strategies to enable the strand-specific sequencing of herpesvirus genomes. The availability of strand-specific sequence data will be used to determine if the emergence of new genotypes of herpesviruses is a result of spontaneous mutation or the selection of existing genetic diversity within a viral isolate. This new knowledge will improve our understanding of this important virus family.
The application of synthetic biology to large DNA viral genomes
The capacity to manipulate the genomes of viruses in the laboratory remains dependent on their in vitro replication efficiency. This limitation has hindered our attempts better understand many of these viruses. This project will address this issue by applying long range PCR and DNA fragment assembly to construction copies of selected herpesviral genomes. The capacity of these genomes to facilitate rescue of infectious virus will also be determined. The availability of these infectious genome copies will aide in the development of improved diagnostics and control methods for these currently difficult to work with viruses.
The application of gene editing to improve animal resistance to infections
Gene editing technologies have enabled the rapid and specific modification of the genomes of complex organisms. Projects are available that will focus on the application of gene editing to increase the resilience of livestock cells to pathogens which reduce productivity. These projects will enable the candidate to gain experience in mammalian cell culture, transfection, growth and titration of viruses, molecular cloning, Western blotting, quantitative real-time PCR, genomic analyses, and fluorescence microscopy. These studies will improve our understanding of host/pathogen interactions towards the development of more resilient livestock.
The construction and evaluation of herpesvirus vectors
Robinson, K. E. and Mahony, T. J. (2020). The construction and evaluation of herpesvirus vectors. Viral vectors in veterinary vaccine development. (pp. 95-110) Cham, Switzerland: Springer International Publishing. doi: 10.1007/978-3-030-51927-8_7
Betaherpesvirinae (Suid Herpesvirus 2)
Mahony, Timothy John (2016). Betaherpesvirinae (Suid Herpesvirus 2). Molecular detection of animal viral pathogens. (pp. 747-752) edited by Dongyou Liu. Boca Raton, FL, United States: CRC Press. doi: 10.1201/b19719-1
Herpesvirus mutagenesis facilitated by infectious bacterial artificial chromosomes (iBACs)
Robinson, Karl E. and Mahony, Timothy J. (2015). Herpesvirus mutagenesis facilitated by infectious bacterial artificial chromosomes (iBACs). Bacterial artificial chromosomes. (pp. 181-197) edited by Kumaran Narayanan. New York, NY, United States: Springer Science+Business Media. doi: 10.1007/978-1-4939-1652-8_8
Mahony, Timothy J., McCarthy, Fiona M., Gravel, Jennifer L. and Young, Peter L. (2005). Construction of a gene inactivation library for Bovine herpesvirus 1 using infectious clone technology. DNA viruses: Methods and protocols. (pp. 387-400) edited by Paul M. Lieberman. Totowa, NJ, United States: Humana Press. doi: 10.1385/1-59259-848-X:387
Skim-Nanopore sequencing for routine genomic evaluation and bacterial pathogen detection in cattle
Lamb, H. J., Nguyen, L. T., Briody, T. E., Ambrose, R. K., Hayes, B. J., Mahony, T. J. and Ross, E. M. (2023). Skim-Nanopore sequencing for routine genomic evaluation and bacterial pathogen detection in cattle. Animal Production Science, 63 (11), 1074-1085. doi: 10.1071/an22451
Statement in Support of: “Virology under the Microscope—a Call for Rational Discourse”
Speck, Peter, Mackenzie, Jason, Bull, Rowena A., Slobedman, Barry, Drummer, Heidi, Fraser, Johanna, Herrero, Lara, Helbig, Karla, Londrigan, Sarah, Moseley, Gregory, Prow, Natalie, Hansman, Grant, Edwards, Robert, Ahlenstiel, Chantelle, Abendroth, Allison, Tscharke, David, Hobson-Peters, Jody, Kriiger-Loterio, Robson, Parry, Rhys, Marsh, Glenn, Harding, Emma, Jacques, David A., Gartner, Matthew J., Lee, Wen Shi, McAuley, Julie, Vaz, Paola, Sainsbury, Frank, Tate, Michelle D., Sinclair, Jane ... Young, Paul (2023). Statement in Support of: “Virology under the Microscope—a Call for Rational Discourse”. mBio, 14 (3), e0081523. doi: 10.1128/mbio.00815-23
Statement in Support of: “Virology under the Microscope—a Call for Rational Discourse”
Speck, Peter, Mackenzie, Jason, Bull, Rowena A., Slobedman, Barry, Drummer, Heidi, Fraser, Johanna, Herrero, Lara, Helbig, Karla, Londrigan, Sarah, Moseley, Gregory, Prow, Natalie, Hansman, Grant, Edwards, Robert, Ahlenstiel, Chantelle, Abendroth, Allison, Tscharke, David, Hobson-Peters, Jody, Kriiger-Loterio, Robson, Parry, Rhys, Marsh, Glenn, Harding, Emma, Jacques, David A., Gartner, Matthew J., Lee, Wen Shi, McAuley, Julie, Vaz, Paola, Sainsbury, Frank, Tate, Michelle D., Sinclair, Jane ... Young, Paul (2023). Statement in Support of: “Virology under the Microscope—a Call for Rational Discourse”. Journal of Virology, 97 (5), e0045123. doi: 10.1128/jvi.00451-23
Statement in Support of: “Virology under the Microscope—a Call for Rational Discourse”
Speck, Peter, Mackenzie, Jason, Bull, Rowena A., Slobedman, Barry, Drummer, Heidi, Fraser, Johanna, Herrero, Lara, Helbig, Karla, Londrigan, Sarah, Moseley, Gregory, Prow, Natalie, Hansman, Grant, Edwards, Robert, Ahlenstiel, Chantelle, Abendroth, Allison, Tscharke, David, Hobson-Peters, Jody, Kriiger-Loterio, Robson, Parry, Rhys, Marsh, Glenn, Harding, Emma, Jacques, David A., Gartner, Matthew J., Lee, Wen Shi, McAuley, Julie, Vaz, Paola, Sainsbury, Frank, Tate, Michelle D., Sinclair, Jane ... Young, Paul (2023). Statement in Support of: “Virology under the Microscope—a Call for Rational Discourse”. mSphere, 8 (3), e0016523. doi: 10.1128/msphere.00165-23
Ambrose, Rebecca K., Blakebrough-Hall, Claudia, Gravel, Jennifer L., Gonzalez, Luciano A. and Mahony, Timothy J. (2023). Characterisation of the upper respiratory tract virome of feedlot cattle and its association with bovine respiratory disease. Viruses, 15 (2) 455. doi: 10.3390/v15020455
Statement in Support of: “Virology under the Microscope-a Call for Rational Discourse”
Speck, Peter, Mackenzie, Jason, Bull, Rowena A., Slobedman, Barry, Drummer, Heidi, Fraser, Johanna, Herrero, Lara, Helbig, Karla, Londrigan, Sarah, Moseley, Gregory, Prow, Natalie, Hansman, Grant, Edwards, Robert, Ahlenstiel, Chantelle, Abendroth, Allison, Tscharke, David, Hobson-Peters, Jody, Kriiger-Loterio, Robson, Parry, Rhys, Marsh, Glenn, Harding, Emma, Jacques, David A., Gartner, Matthew J., Lee, Wen Shi, McAuley, Julie, Vaz, Paola, Sainsbury, Frank, Tate, Michelle D., Sinclair, Jane ... Young, Paul (2023). Statement in Support of: “Virology under the Microscope-a Call for Rational Discourse”. Journal of Virology, 97 (5). doi: 10.1128/jvi.00451-23
RNAi-Based Biocontrol of Pests to Improve the Productivity and Welfare of Livestock Production
Menezes, Pia S., Yan, Yakun, Yang, Yunjia, Mitter, Neena, Mahony, Timothy J. and Mody, Karishma T. (2022). RNAi-Based Biocontrol of Pests to Improve the Productivity and Welfare of Livestock Production. Applied Biosciences, 1 (3), 229-243. doi: 10.3390/applbiosci1030015
Salgadu, Ayesha, Cheung, Allison, Schibrowski, Meghan L., Wawegama, Nadeeka K., Mahony, Timothy J., Stevenson, Mark A., Browning, Glenn F., Barnes, Tamsin S. and Firestone, Simon M. (2022). Bayesian latent class analysis to estimate the optimal cut-off for the MilA ELISA for the detection of Mycoplasma bovis antibodies in sera, accounting for repeated measures. Preventive Veterinary Medicine, 205 105694, 1-8. doi: 10.1016/j.prevetmed.2022.105694
Factors associated with bovine respiratory disease case fatality in feedlot cattle
Blakebrough-Hall, Claudia, Hick, Paul, Mahony, T. J. and González, Luciano A. (2022). Factors associated with bovine respiratory disease case fatality in feedlot cattle. Journal of Animal Science, 100 (1) skab361. doi: 10.1093/jas/skab361
Investigation of Campylobacter colonization in three Australian commercial free-range broiler farms
Pumtang-On, Pongthorn, Mahony, Timothy J., Hill, Rodney A., Pavic, Anthony and Vanniasinkam, Thiru (2021). Investigation of Campylobacter colonization in three Australian commercial free-range broiler farms. Poultry Science, 100 (3) 100891, 1-12. doi: 10.1016/j.psj.2020.12.004
A systematic review of Campylobacter jejuni vaccine candidates for chickens
Pumtang-on, Pongthorn, Mahony, Timothy J., Hill, Rodney A. and Vanniasinkam, Thiru (2021). A systematic review of Campylobacter jejuni vaccine candidates for chickens. Microorganisms, 9 (2) 397, 1-48. doi: 10.3390/microorganisms9020397
Lacasta, Anna, Mody, Karishma T, De Goeyse, Ine, Yu, Chengzhong, Zhang, Jun, Nyagwange, James, Mwalimu, Stephen, Awino, Elias, Saya, Rosemary, Njoroge, Thomas, Muriuki, Robert, Ndiwa, Nicholas, Poole, Elisabeth Jane, Zhang, Bing, Cavallaro, Antonino, Mahony, Timothy J, Steinaa, Lucilla, Mitter, Neena and Nene, Vishvanath (2021). Synergistic effect of two nanotechnologies enhances the protective capacity of the Theileria parva Sporozoite p67C antigen in cattle. Journal of Immunology, 206 (4), 686-699. doi: 10.4049/jimmunol.2000442
Mody, Karishma T., Zhang, Bing, Li, Xun, Fletcher, Nicholas L., Akhter, Dewan T., Jarrett, Sandy, Zhang, Jun, Yu, Chengzhong, Thurecht, Kristofer J., Mahony, Timothy J and Mitter, Neena (2021). Characterization of the biodistribution of a silica vesicle Nanovaccine carrying a Rhipicephalus (Boophilus) microplus protective antigen with in vivo live animal imaging. Frontiers in Bioengineering and Biotechnology, 8 606652, 1-11. doi: 10.3389/fbioe.2020.606652
Genome sequences of two marsupial simplex viruses, macropodid alphaherpesviruses 2 and 4
Vaz, P. K., Mahony, T. J., Hartley, C. A., Motha, J. and Devlin, J. M. (2021). Genome sequences of two marsupial simplex viruses, macropodid alphaherpesviruses 2 and 4. Microbiology Resource Announcements, 10 (2) e01189-20. doi: 10.1128/mra.01189-20
Angsujinda, Kitipong, Mahony, Timothy J., Smith, Duncan R., Kettratad, Jes and Assavalapsakul, Wanchai (2020). Expression profile of selected genes of the E-11 cell line in response to red-spotted grouper nervous necrosis virus infection. Aquaculture Reports, 18 100468, 100468. doi: 10.1016/j.aqrep.2020.100468
Devi, Aruna, Mahony, Timothy J., Wilkinson, Jenny M. and Vanniasinkam, Thiru (2019). Antimicrobial susceptibility of clinical isolates of Campylobacter jejuni from New South Wales, Australia. Journal of Global Antimicrobial Resistance, 16, 76-80. doi: 10.1016/j.jgar.2018.09.011
Chen, Weiyu, Zuo, Huali, Rolfe, Barbara, Schembri, Mark A., Cobbold, Rowland N., Zhang, Bing, Mahony, Timothy J. and Xu, Zhi Ping (2018). Clay nanoparticles co-deliver three antigens to promote potent immune responses against pathogenic Escherichia coli. Journal of Controlled Release, 292, 196-209. doi: 10.1016/j.jconrel.2018.11.008
Modulation of bovine herpesvirus 1 infection by virally encoded microRNAs
Kanokudom, Sitthichai, Mahony, Timothy J., Smith, Duncan R. and Assavalapsakul, Wanchai (2018). Modulation of bovine herpesvirus 1 infection by virally encoded microRNAs. Virus Research, 257, 1-6. doi: 10.1016/j.virusres.2018.08.022
Mycoplasma bovis and bovine respiratory disease: a risk factor study in Australian feeder cattle
Schibrowski, M. L., Gibson, J. S., Hay, K. E., Mahony, T. J. and Barnes, T. S. (2018). Mycoplasma bovis and bovine respiratory disease: a risk factor study in Australian feeder cattle. Preventive Veterinary Medicine, 157, 152-161. doi: 10.1016/j.prevetmed.2018.06.005
Chen, Weiyu, Zuo, Huali, Li, Bei, Duan, Chengcheng, Rolfe, Barbara, Zhang, Bing, Mahony, Timothy J and Xu, Zhi Ping (2018). Clay nanoparticles elicit long-term immune responses by forming biodegradable depots for sustained antigen stimulation. Small, 14 (19) 1704465, e1704465. doi: 10.1002/smll.201704465
Schibrowski, Meghan L., Barnes, Tamsin S., Wawegama, Nadeeka K., Vance, Megan E., Markham, Philip F., Mansell, Peter D., Marenda, Marc S., Kanci, Anna, Perez-Casal, José, Browning, Glenn F., Gibson, Justine S. and Mahony, Timothy J. (2018). The performance of three immune assays to assess the serological status of cattle experimentally exposed to mycoplasma bovis. Veterinary Sciences , 5 (1) 27, 27. doi: 10.3390/vetsci5010027
In Vivo Characterisation of Five Strains of Bovine Viral Diarrhoea Virus 1 (Subgenotype 1c)
Ambrose, Rebecca K, Gravel, Jennifer L, Commins, Margaret A, Fowler, Elizabeth V and Mahony, Timothy J (2018). In Vivo Characterisation of Five Strains of Bovine Viral Diarrhoea Virus 1 (Subgenotype 1c). Pathogens (Basel, Switzerland), 7 (1) 12, 1-18. doi: 10.3390/pathogens7010012
Chen, Weiyu, Zuo, Huali, Mahony, Timothy J, Zhang, Bing, Rolfe, Barbara and Xu, Zhi Ping (2017). Efficient induction of comprehensive immune responses to control pathogenic E. coli by clay nano-adjuvant with the moderate size and surface charge. Scientific reports, 7 (1) 13367, 1-12. doi: 10.1038/s41598-017-13570-2
Population-level effects of risk factors for bovine respiratory disease in Australian feedlot cattle
Hay, K. E., Morton, J. M., Clements, A. C. A., Mahony, T. J. and Barnes, T. S. (2017). Population-level effects of risk factors for bovine respiratory disease in Australian feedlot cattle. Preventive Veterinary Medicine, 140, 78-86. doi: 10.1016/j.prevetmed.2017.03.001
Zhang, Bing, Cavallaro, Antonio S., Mody, Karishma T., Zhang, Jun, Deringer, James R., Brown, Wendy C., Mahony, Timothy J., Yu, Chengzhong and Mitter, Neena (2016). Nanoparticle-based delivery of Anaplasma marginale membrane proteins, VirB9-1 and VirB10 produced in the Pichia pastoris expression system. Nanomaterials, 6 (11) 201, 201. doi: 10.3390/nano6110201
Alphaherpesvirus-associated disease in greater bilbies (Macrotis lagotis)
Besier, A. S., Mahony, T. J., Crockford, M., Gravel, J. L., Chapman, T. F. and O'Dea, M. A. (2016). Alphaherpesvirus-associated disease in greater bilbies (Macrotis lagotis). Australian Veterinary Journal, 94 (6), 208-212. doi: 10.1111/avj.12447
Hay, K. E., Morton, J. M., Clements, A. C. A., Mahony, T. J. and Barnes, T. S. (2016). Associations between feedlot management practices and bovine respiratory disease in Australian feedlot cattle. Preventive Veterinary Medicine, 128, 23-32. doi: 10.1016/j.prevetmed.2016.03.017
Associations between exposure to viruses and bovine respiratory disease in Australian feedlot cattle
Hay, K. E., Barnes, T. S., Morton, J. M., Gravel, J. L., Commins, M. A., Horwood, P. F., Ambrose, R. C., Clements, A. C. A. and Mahony, T. J. (2016). Associations between exposure to viruses and bovine respiratory disease in Australian feedlot cattle. Preventive Veterinary Medicine, 127, 121-133. doi: 10.1016/j.prevetmed.2016.01.024
Hay, K. E., Morton, J. M., Schibrowski, M. L., Clements, A. C. A., Mahony, T. J. and Barnes, T. S. (2016). Associations between prior management of cattle and risk of bovine respiratory disease in feedlot cattle. Preventive Veterinary Medicine, 127, 37-43. doi: 10.1016/j.prevetmed.2016.02.006
Wawegama, Nadeeka K., Markham, Philip F., Kanci, Anna, Schibrowski, Meghan, Oswin, Sally, Barnes, Tamsin S., Firestone, Simon M., Mahony, Timothy J. and Browning, Glenn F. (2016). Evaluation of an IgG enzyme-linked immunosorbent assay as a serological assay for detection of mycoplasma bovis infection in feedlot cattle. Journal of Clinical Microbiology, 54 (5), 1269-1275. doi: 10.1128/JCM.02492-15
Untangling the complexity of diseases such as calf diarrhoea is crucial to future productivity
Mahony, Timothy J. (2016). Untangling the complexity of diseases such as calf diarrhoea is crucial to future productivity. Veterinary Journal, 210, 3-4. doi: 10.1016/j.tvjl.2015.11.005
Chen, Weiyu, Zhang, Bing, Mahony, Timothy, Gu, Wenyi, Rolfe, Barbara and Xu, Zhi Ping (2016). Efficient and durable vaccine against Intimin β of Diarrheagenic E. Coli induced by clay nanoparticles. Small, 12 (12), 1627-1639. doi: 10.1002/smll.201503359
Hay, K. E., Morton, J. M., Mahony, T. J., Clements, A. C. A. and Barnes, T. S. (2016). Associations between animal characteristic and environmental risk factors and bovine respiratory disease in Australian feedlot cattle. Preventive Veterinary Medicine, 125, 66-74. doi: 10.1016/j.prevetmed.2016.01.013
Hay, K. E., Ambrose, R. C. K., Morton, J. M., Horwood, P. F., Gravel, J. L., Waldron, S., Commins, M. A., Fowler, E. V., Clements, A. C. A., Barnes, T. S. and Mahony, T. J. (2016). Effects of exposure to Bovine viral diarrhoea virus 1 on risk of bovine respiratory disease in Australian feedlot cattle. Preventive Veterinary Medicine, 126, 159-169. doi: 10.1016/j.prevetmed.2016.01.025
The first genome sequence of a metatherian herpesvirus: Macropodid herpesvirus 1
Vaz, Paola K., Mahony, Timothy J., Hartley, Carol A., Fowler, Elizabeth V., Ficorilli, Nino, Lee, Sang W., Gilkerson, James R., Browning, Glenn F. and Devlin, Joanne M. (2016). The first genome sequence of a metatherian herpesvirus: Macropodid herpesvirus 1. BMC Genomics, 17 (1) 70, 70. doi: 10.1186/s12864-016-2390-2
Mody, Karishma T., Mahony, Donna, Cavallaro, Antonino S., Zhang, Jun, Zhang, Bing, Mahony, Timothy J., Yu, Chengzhong and Mitter, Neena (2016). Correction: Silica Vesicle Nanovaccine Formulations Stimulate Long-Term Immune Responses to the Bovine Viral Diarrhoea Virus E2 Protein (vol 10, e0143507, 2015). Plos One, 11 (1) e0146631, 1-1. doi: 10.1371/journal.pone.0146631
Prevalence of Campylobacter spp. in diarrhoea samples from patients in New South Wales, Australia
Devi, Aruna, Gunjilac, Adam S., Wilkinson, Jenny M., Vanniasinkam, Thiru and Mahony, Timothy J. (2016). Prevalence of Campylobacter spp. in diarrhoea samples from patients in New South Wales, Australia. International Microbiology, 19 (1), 33-37. doi: 10.2436/20.1501.01.261
Mody, Karishma T., Mahony, Donna, Cavallaro, Antonino S., Zhang, Jun, Zhang, Bing, Mahony, Timothy J., Yu, Chengzhong and Mitter, Neena (2015). Silica vesicle nanovaccine formulations stimulate long-term immune responses to the bovine viral diarrhoea virus e2 protein. PLoS One, 10 (12) e0143507, 1-16. doi: 10.1371/journal.pone.0143507
Mahony, Donna, Mody, Karishma T., Cavallaro, Antonino S., Hu, Qiuhong, Mahony, Timothy J., Qiao, Shizhang and Mitter, Neena (2015). Immunisation of Sheep with Bovine Viral Diarrhoea Virus, E2 Protein Using a Freeze-Dried Hollow Silica Mesoporous Nanoparticle Formulation. Plos One, 10 (11) e0141870, e0141870. doi: 10.1371/journal.pone.0141870
Characterisation of the Equine adenovirus 2 genome
Giles, Carla, Vanniasinkam, Thiru, Barton, Mary and Mahony, Timothy J. (2015). Characterisation of the Equine adenovirus 2 genome. Veterinary Microbiology, 179 (3-4), 184-189. doi: 10.1016/j.vetmic.2015.07.024
Mahony, T. J. (2015). Understanding the molecular basis of disease is crucial to improving the design and construction of herpesviral vectors for veterinary vaccines. Vaccine, 33 (44), 5897-5904. doi: 10.1016/j.vaccine.2015.09.028
Identification of non-essential loci within the Meleagrid herpesvirus 1 genome
Hall, Robyn N., Meers, Joanne, Fowler, Elizabeth V. and Mahony, Timothy J. (2015). Identification of non-essential loci within the Meleagrid herpesvirus 1 genome. Virology Journal, 12 (1) 130, 130.1-130.12. doi: 10.1186/s12985-015-0362-9
Mahony, Timothy J., Hall, Robyn N., Walkden-Brown, Stephen, Meers, Joanne, Gravel, Jennifer L., West, Lani, Hardy, Vanessa, Islam, A. F. M. Fakhrul, Fowler, Elizabeth V. and Mitter, Neena (2015). Genomic deletions and mutations resulting in the loss of eight genes reduce the in vivo replication capacity of Meleagrid herpesvirus 1. Virus Genes, 51 (1), 85-95. doi: 10.1007/s11262-015-1216-7
Goldspink, L. K., Mollinger, J. L., Barnes, T. S., Groves, M., Mahony, T. J. and Gibson, J. S. (2015). Antimicrobial susceptibility of Histophilus somni isolated from clinically affected cattle in Australia. Veterinary Journal, 203 (2), 239-243. doi: 10.1016/j.tvjl.2014.12.008
Detection of Campylobacter in human faecal samples in Fiji
Devi, Aruna, Wilkinson, Jenny, Mahony, Timothy and Vanniasinkam, Thiru (2014). Detection of Campylobacter in human faecal samples in Fiji. Western Pacific Surveillance and Response Journal, 5 (4), 30-33. doi: 10.5365/WPSAR.2014.5.2.007
Mody, Karishma T., Mahony, Donna, Zhang, Jun, Cavallaro, Antonino S., Zhang, Bing, Popat, Amirali, Mahony, Timothy J., Yu, Chengzhong and Mitter, Neena (2014). Silica vesicles as nanocarriers and adjuvants for generating both antibody and T-cell mediated immune resposes to Bovine Viral Diarrhoea Virus E2 protein. Biomaterials, 35 (37), 9972-9983. doi: 10.1016/j.biomaterials.2014.08.044
Hay, K. E., Barnes, T. S., Morton, J. M., Clements, A. C. A. and Mahony, T. J. (2014). Risk factors for bovine respiratory disease in Australian feedlot cattle: use of a causal diagram-informed approach to estimate effects of animal mixing and movements before feedlot entry. Preventive Veterinary Medicine, 117 (1), 160-169. doi: 10.1016/j.prevetmed.2014.07.001
Easton, Donna M., Allsopp, Luke P., Phan, Minh-Duy, Gomes Moriel, Danilo, Goh, Guan Kai, Beatson, Scott A., Mahony, Timothy J., Cobbold, Rowland N. and Schembri, Mark A. (2014). The intimin-like protein FdeC is regulated by H-NS and temperature in Enterohaemorrhagic Escherichia coli. Applied and Environmental Microbiology, 80 (23), 7337-7347. doi: 10.1128/AEM.02114-14
Mody, Karishma T., Mahony, Donna, Cavallaro, Antonino S., Stahr, Frances, Qiao, Shi Zhang, Mahony, Timothy J. and Mitter, Neena (2014). Freeze-drying of ovalbumin loaded mesoporous silica nanoparticle vaccine formulation increases antigen stability under ambient conditions. International Journal of Pharmaceutics, 465 (1-2), 325-332. doi: 10.1016/j.ijpharm.2014.01.037
Mahony, D., Cavallaro, A. S., Mody, K. T., Xiong, L., Mahony, T. J., Qiao, S. Z. and Mitter, Neena (2014). In vivo delivery of bovine viral diahorrea virus, E2 protein using hollow mesoporous silica nanoparticles. Nanoscale, 6 (12), 6617-6626. doi: 10.1039/C4NR01202J
Biosecurity strategies for the beef industry
Mahony, Timothy J. (2014). Biosecurity strategies for the beef industry. Australasian Biotechnology, 24 (3), 60-60.
Is Mycoplasma bovis a missing component of the bovine respiratory disease complex in Australia?
Horwood, P. F., Schibrowski, M. L., Fowler, E. V., Gibson, J. S., Barnes, T. S. and Mahony, T. J. (2014). Is Mycoplasma bovis a missing component of the bovine respiratory disease complex in Australia?. Australian Veterinary Journal, 92 (6), 185-191. doi: 10.1111/avj.12184
Mesoporous silica nanoparticles act as a self-adjuvant for ovalbumin model antigen in mice
Mahony, Donna, Cavallaro, Antonino S., Stahr, Frances, Mahony, Timothy J., Qiao, Shi Zhang and Mitter, Neena (2013). Mesoporous silica nanoparticles act as a self-adjuvant for ovalbumin model antigen in mice. Small, 9 (18), 3138-3146. doi: 10.1002/smll.201300012
The meleagrid herpesvirus 1 genome is partially resistant to transposition
Hall, Robyn N., Meers, Joanne, Mitter, Neena, Fowler, Elizabeth V. and Mahony, Timothy J. (2013). The meleagrid herpesvirus 1 genome is partially resistant to transposition. Avian Diseases, 57 (2 SUPPL. 1), 380-386. doi: 10.1637/10339-082912-Reg.1
A virological investigation into declining woylie populations
Pacioni, Carlo, Johansen, Cheryl A., Mahony, Timothy J., O'Dea, Mark A., Robertson, Ian D., Wayne, Adrian F. and Ellis, Trevor (2013). A virological investigation into declining woylie populations. Australian Journal of Zoology, 61 (6), 446-453. doi: 10.1071/ZO13077
Expression of Two N1 Clones with Single Amino Acid Dissimilarity of Avian Influenza H5N1 Virus
Hartawan, Risza, Dharmayanti, Ni Luh Putu Indi, Robinson, Karl, Mahony, Timothy and Meers, Joanne (2012). Expression of Two N1 Clones with Single Amino Acid Dissimilarity of Avian Influenza H5N1 Virus. HAYATI Journal of Biosciences, 19 (4), 169-176. doi: 10.4308/hjb.19.4.169
Freeze-drying of protein-loaded nanoparticles for vaccine delivery
Mody, Karishma, Mahony, Donna, Mahony, Tim and Mitter, Neena (2012). Freeze-drying of protein-loaded nanoparticles for vaccine delivery. Drug Delivery Letters, 2 (2), 83-91. doi: 10.2174/2210304x11202020083
Back to BAC: The use of infectious clone technologies for viral mutagenesis
Hall, Robyn N., Meers, Joanne, Fowler, Elizabeth and Mahony, Timothy (2012). Back to BAC: The use of infectious clone technologies for viral mutagenesis. Viruses, 4 (2), 211-235. doi: 10.3390/v4020211
Genetic characterization of equine adenovirus type 1
Cavanagh, Heather M. A., Mahony, Timothy J. and Vanniasinkam, Thiru (2012). Genetic characterization of equine adenovirus type 1. Veterinary Microbiology, 155 (1), 33-37. doi: 10.1016/j.vetmic.2011.08.014
Cavallaro, Antonio S., Mahony, Donna, Commins, Margaret, Mahony, Timothy J. and Mitter, Neena (2011). Endotoxin-free purification for the isolation of Bovine Viral Diarrhoea Virus E2 protein from insoluble inclusion body aggregates. Microbial Cell Factories, 10 (1) 57, 57.1-57.9. doi: 10.1186/1475-2859-10-57
Hartawan, Risza, Robinson, K., Mahony, T. and Meers, J. (2011). In vitro expression of native H5 and N1 genes of avian influenza virus by using Green Fluorescent Protein as reporter. Indonesian Journal of Animal and Veterinary Sciences, 16 (3), 234-241. doi: 10.14334/jitv.v16i3.618
Easton, Donna M., Totsika, Makrina, Allsopp, Luke P., Phan, Minh-Duy, Idris, Adi, Wurpel, Daniël J., Sherlock, Orla, Zhang, Bing, Venturini, Carola, Beatson, Scott A., Mahony, Timothy J., Cobbold, Rowland N. and Schembri, Mark A. (2011). Characterization of EhaJ, a new autotransporter protein from enterohemorrhagic and enteropathogenic Escherichia coli. Frontiers in Microbiology: Frontiers in Cellular and Infection Microbiology, 2 (JUNE), 120.1-120.9. doi: 10.3389/fmicb.2011.00120
Horwood, Paul Francis and Mahony, Timothy John (2011). Multiplex real-time RT-PCR detection of three viruses associated with the bovine respiratory disease complex. Journal of Virological Methods, 171 (2), 360-363. doi: 10.1016/j.jviromet.2010.11.020
Polyvalent Alphaherpesvirus vectors in veterinary vaccine research
Robinson, K. E., Meers, J. and Mahony, T. J. (2010). Polyvalent Alphaherpesvirus vectors in veterinary vaccine research. Current Topics in Virology, 8, 71-88.
Mahony, Timothy J. (2010). Bovine herpesvirus: What is missing from our understanding of the relationship between BoHV-1 and BoHV-5?. Veterinary Journal, 184 (2), 124-125. doi: 10.1016/j.tvjl.2009.06.020
Hartawan, Risza, Robinson, Karl, Mahony, Timothy and Meers, Joanne (2010). Characterisation of the H5 and N1 genes of an Indonesian highly pathogenic avian influenza virus isolate by sequencing of multiple clone approach. Indonesian Journal of Animal and Veterinary Sciences, 15 (3), 240-251. doi: 10.14334/jitv.v15i3.663
Characterization of microRNAs encoded by the bovine herpesvirus 1 genome
Glazov, Evgeny A., Horwood, Paul F., Assavalapsakul, Wanchai, Kongsuwan, Kritaya, Mitchell, Roger W., Mitter, Neena and Mahony, Timothy J. (2010). Characterization of microRNAs encoded by the bovine herpesvirus 1 genome. Journal of General Virology, 91 (1), 32-41. doi: 10.1099/vir.0.014290-0
Molecular detection and phylogenetic analysis of betanodavirus isolates from Australian finfish
Moody, N. J. G., Horwood, P. F., Reynolds, A., Mahony, T. J., Anderson, I. G. and Oakey, H. J. (2009). Molecular detection and phylogenetic analysis of betanodavirus isolates from Australian finfish. Diseases of Aquatic Organisms, 87 (3), 151-160. doi: 10.3354/dao02130
Elkington, R. A., Humphries, M., Commins, M., Maugeri, N., Tierney, T. and Mahony, T. J. (2009). A Lucilia cuprina excretory-secretory protein inhibits the early phase of lymphocyte activation and subsequent proliferation. Parasite Immunology, 31 (12), 750-765. doi: 10.1111/j.1365-3024.2009.01154.x
Repertoire of bovine miRNA and miRNA-like small regulatory RNAs expressed upon viral infection
Glazov, Evgeny A., Kongsuwan, Kritaya, Assavalapsakul, Wanchai, Horwood, Paul F., Mitter, Neena and Mahony, Timothy J. (2009). Repertoire of bovine miRNA and miRNA-like small regulatory RNAs expressed upon viral infection. PLoS One, 4 (7) e6349, e6349-1-e6349-6. doi: 10.1371/journal.pone.0006349
Understanding animal viruses using the Gene Ontology
McCarthy, Fiona M., Mahony, Timothy J., Mark S. Parcells and Shane C. Burgess (2009). Understanding animal viruses using the Gene Ontology. Trends in Microbiology, 17 (7), 328-335. doi: 10.1016/j.tim.2009.04.006
The essential and non-essential genes of Bovine herpesvirus 1
Robinson, Karl E., Meers, Joanne, Gravel, Jennifer L., McCarthy, Fiona M. and Mahony, Timothy J. (2008). The essential and non-essential genes of Bovine herpesvirus 1. Journal of General Virology, 89 (11), 2851-2863. doi: 10.1099/vir.0.2008/002501-0
Identification of two distinct bovine parainfluenza virus type 3 genotypes
Horwood, Paul Francis, Gravel, Jennifer Lillian and Mahony, Timothy John (2008). Identification of two distinct bovine parainfluenza virus type 3 genotypes. Journal of General Virology, 89 (7), 1643-1648. doi: 10.1099/vir.0.2008/000026-0
A blowfly strike vaccine requires an understanding of host-pathogen interactions
Elkington, Rebecca A. and Mahony, Timothy J. (2007). A blowfly strike vaccine requires an understanding of host-pathogen interactions. Vaccine, 25 (28), 5133-5145. doi: 10.1016/j.vaccine.2007.04.074
Islam, Aminul, Cheetham, Brian F., Mahony, Timothy J., Young, Peter L. and Walkden-Brown, Stephen W. (2006). Absolute quantitation of Marek's disease virus and Herpesvirus of turkeys in chicken lymphocyte, feather tip and dust samples using real-time PCR. Journal of Virological Methods, 132 (1-2), 127-134. doi: 10.1016/j.jviromet.2005.10.009
Genetic analysis of bovine viral diarrhoea viruses from Australia
Mahony, Timothy J., McCarthy, Fiona M., Gravel, Jennifer L., Corney, Bruce, Young, Peter L. and Vilcek, Stefan (2005). Genetic analysis of bovine viral diarrhoea viruses from Australia. Veterinary Microbiology, 106 (1-2), 1-6. doi: 10.1016/j.vetmic.2004.10.024
Mahony, Timothy J, McCarthy, Fiona M, Gravel, Jennifer L and Young, Peter L (2005). Construction of a gene inactivation library for Bovine herpesvirus 1 using infectious clone technology. Methods in molecular biology (Clifton, N.J.), 292, 387-400.
Islam, Aminul, Harrison, Bruce, Cheetham, Brian F., Mahony, Timothy J., Young, Peter L. and Walkden-Brown, Stephen W. (2004). Differential amplification and quantification of Marek’s Disease viruses using real-time polymerase chain reaction. Journal of Virological Methods, 119 (2), 103-113. doi: 10.1016/j.jviromet.2004.03.006
Rapid and efficient construction of recombinant bovine herpesvirus 1 genomes
Mahony, T. J., McCarthy, F. M., Gravel, J. L. and Young, P. L. (2003). Rapid and efficient construction of recombinant bovine herpesvirus 1 genomes. Journal of Virological Methods, 107 (2), 269-274. doi: 10.1016/S0166-0934(02)00226-4
Mahony, Timothy J., McCarthy, Fiona M., Gravel, Jennifer L., West, Lani and Young, Peter L. (2002). Construction and manipulation of an infectious clone of the bovine herpesvirus 1 genome maintained as a bacterial artificial chromosome. Journal of Virology, 76 (13), 6660-6668. doi: 10.1128/JVI.76.13.6660-6668.2002
Macropodid herpesvirus 1 encodes genes for both thymidylate synthase and ICP34.5
Guliani, Sandeep, Polkinghorne, Ian, Smith, Greg A., Young, Peter, Mattick, John S. and Mahony, Timothy J. (2002). Macropodid herpesvirus 1 encodes genes for both thymidylate synthase and ICP34.5. Virus Genes, 24 (3), 207-213. doi: 10.1023/A:1015396430131
Primary cell cultures isolated from Penaeus monodon prawns
West, L., Mahony, T., McCarthy, F., Watanabe, J., Hewitt, D. and Hansford, S. (1999). Primary cell cultures isolated from Penaeus monodon prawns. Methods in Cell Science, 21 (4), 219-223. doi: 10.1023/A:1009864212013
GulianiS, SmithGA, YoungPL, MattickJS and Mahony, T. J. (1999). Reactivation of a macropodid herpesvirus from the eastern grey kangaroo (Macropus giganteus) following corticosteroid treatment. Veterinary Microbiology, 68 (1-2), 59-69. doi: 10.1016/S0378-1135(99)00061-9
Mahony, T.J., Smith, G.A. and Thomson, D.M. (1999). Macropodid herpesviruses 1 and 2 occupy unexpected molecular phylogenic positions within the Alphaherpesvirinae. Journal of General Virology, 80 (2), 433-436. doi: 10.1099/0022-1317-80-2-433
Mahony, Timothy J. and Miller, David J. (1998). Linkage of genes encoding enolase (eno) and CTP synthase (pyrG) in the beta-subdivision proteobacterium Nitrosomonas europaea. FEMS Microbiology Letters, 165 (1), 153-157. doi: 10.1111/j.1574-6968.1998.tb13140.x
Quaternary structure of the hydroxylamine oxidoreductase from Nitrosomonas europaea
Hoppert, Michael, Mahony, Timothy J., Mayer, Frank and Miller, David J. (1995). Quaternary structure of the hydroxylamine oxidoreductase from Nitrosomonas europaea. Archives of Microbiology, 163 (4), 300-306. doi: 10.1007/BF00393384
Miller, D.J., Harrison, P.L., Mahony, T.J., McMillan, J.P., Miles, A., Odorico, D.M. and Ten Lohuis, M.R. (1993). Nucleotide sequence of the histone gene cluster in the coral Acropora formosa (Cnidaria, Scleractinia): features of histone gene structure and organization are common to diploblastic and triploblastic metazoans. Journal of Molecular Evolution, 37 (3), 245-253.
McMillan, J., Mahony, T., Veron, J. E. N. and Miller, D. J. (1991). Nucleotide sequencing of highly repetitive DNA from seven species in the coral genus Acropora (Cnidaria: Scleractinia) implies a division contrary to morphological criteria. Marine Biology, 110 (3), 323-327. doi: 10.1007/BF01344350
Miller, D.J., McMillan, J., Miles, A., ten Lohuis, M. and Mahony, T. (1990). Nucleotide sequence of the histone H3-encoding gene from the scleractinian coral Acropora formosa (cnidaria: scleractinia). Gene, 93 (2), 319-320. doi: 10.1016/0378-1119(90)90243-K
Topical RNAi for Sustainable Animal Health
Mody, Karishma T., Zhang, Bing, Li, Xun, Jain, Ritesh, Li, Peng, James, Peter, Mahony, Timothy J., Xu, Zhiping and Mitter, Neena (2020). Topical RNAi for Sustainable Animal Health. TropAg 2019, Brisbane, QLD Australia, 11-13 November 2019. Basel, Switzerland: MDPI. doi: 10.3390/proceedings2019036170
Vance, Megan, Mahony, Timothy, Easton, Donna, Alikhan, Nabil, Beatson, Scott, Schembri, Mark and Cobbold, Rowland (2014). Inoculation of cattle with novel Escherichia coli antigens reduces enteric colonisation by enterohaemorrhagic E. coli O26. Australian Society for Microbiology Annual Scientific Meeting, Melbourne, VIC, Australia, 5 - 9 July 2014.
Vectoring of avian influenza antigens using a recombinant avian herpes virus vector
Hartawan, Risza, Meers, Joanne, Mahony, Tim and Robinson, Karl (2010). Vectoring of avian influenza antigens using a recombinant avian herpes virus vector. Global Biosecurity 2010, Brisbane, QLD, Australia, 28 February-3 March 2010.
Using immunology and resistant sheep to beat the fly
Colditz, Ian G., Mahony, T. and Elkington, R. (2006). Using immunology and resistant sheep to beat the fly.
Clark, K. L., Mahony, T. and Mackenzie, J. S. (2002). Development of a novel genetic vaccine delivery method utilising a truncated bovine herpesvirus 1 VP22 protein. The World of Microbes, XIIth Int Congress of Virology, Paris, 25 July - 1st August, 2002.
Characterisation of bovine herpesvirus-1 VP22 protein for potential use in vaccine development
Clark, K., Mackenzie, J. S. and Mahony, T. (2001). Characterisation of bovine herpesvirus-1 VP22 protein for potential use in vaccine development. 1st Australian Virology Group Meeting, Fraser Island, December, 2001.
Primary cell cultures isolated from Penaeus monodon prawns
West, Lani, Mahony, T., McCarthy, F., Watanabe, J., Hewitt, D. and Hansford, S. (1999). Primary cell cultures isolated from Penaeus monodon prawns. doi: 10.1023/A:1009864212013
Guliani, Sandeep, Smith, Gregory A., Young, Peter L., Mattick, John S. and Mahony, Timothy J. (1999). Reactivation of a macropodid herpesvirus from the eastern grey kangaroo (Macropus giganteus) following corticosteroid treatment. doi: 10.1016/S0378-1135(99)00061-9
Genetic analysis of Australian bovine viral diarrhoea viruses and bovine herpesviruses
Mahony, T., McCarthy, F., Gravel, J., Clark, K. L., Wilks, C., Mackenzie, J. S. and Young, P. (1999). Genetic analysis of Australian bovine viral diarrhoea viruses and bovine herpesviruses. 11th International Congress of Virology, Sydney, 9-13 August 1999. Aust.: Int. Union of Microbiological Societies.
Evaluation of a prototype vaccine for rattles in foals
(2023–2025) Agrifutures Australia
Saving the endangered Spectacled flying fox from annual tick paralysis mortality events:
(2023–2025) Threatened Species Research Grants
Harnessing the synbio potential of Australia's stingless bees, the first step
(2023–2024) Sugar Research Australia Limited
Development of a novel assay for the detection of the bovine SRY transcript
(2023) NBRYO PTY LTD
On-farm genomics: genomic solutions for Northern beef cattle management and breeding
(2022–2026) Meat & Livestock Australia
(2022–2023) University of Adelaide
Evaluation of anti-tick vaccines for tick immunological control in cattle
(2020–2021) Meat & Livestock Australia
(2019) UQ Major Equipment and Infrastructure
Development of a single shot immunocontraceptive vaccine for cattle
(2017–2024) Meat & Livestock Australia
(2017–2023) Meat & Livestock Australia
Testing and verification of a single-dose cattle tick vaccine
(2016–2019) Meat & Livestock Australia
(2015) UQ Collaboration and Industry Engagement Fund - Seed Research Grant
Live viral vaccines for bovine respiratory disease: BVDV efficacy
(2012–2016) Meat & Livestock Australia
BioClay - Managing biotic stresses from field to market
(2012–2014) Bill & Melinda Gates Foundation
(2012–2014) Queensland Government Smart Futures Research Partnerships Program
Application of herpes viral vectors for in ovo delivery
(2011–2014) Queensland Department of Employment, Economic Development and Innovation
The function of microRNAs encoded within the Bovine herpesvirus 1 origin of replication
(2011–2012) UQ New Staff Research Start-Up Fund
(2010–2015) Meat & Livestock Australia
The Development of a Novel Approach for the Differentiation of Bovine herpesvirus 1 field and vaccine strains
Master Philosophy — Principal Advisor
Other advisors:
The application of genome editing to improve the disease resilience of livestock species
Doctor Philosophy — Principal Advisor
Other advisors:
Identification of the host and viral transcripts that are post-transcriptionally regulated by the microRNAs encoded by bovine herpesvirus 1
Doctor Philosophy — Principal Advisor
Other advisors:
Innovative RNAi for ectoparasites of livestock
Doctor Philosophy — Associate Advisor
Other advisors:
Topical RNAi for sustainable Animal Health
Doctor Philosophy — Associate Advisor
Other advisors:
(2021) Doctor Philosophy — Principal Advisor
Immunological Difference In Bovine Respiratory Disease Susceptibility
(2020) Doctor Philosophy — Principal Advisor
Other advisors:
(2014) Doctor Philosophy — Principal Advisor
Other advisors:
(2009) Doctor Philosophy — Principal Advisor
Application of clay nanoparticles as efficient adjuvant in vaccine against pathogenic diarrheagenic E. Coli
(2018) Doctor Philosophy — Associate Advisor
Other advisors:
Development of Nanoparticle based Vaccine Delivery Systems
(2015) Doctor Philosophy — Associate Advisor
Other advisors:
Epidemiology of bovine respiratory disease in Australian feedlot cattle
(2015) Doctor Philosophy — Associate Advisor
Mycoplasma bovis in Australian feeder cattle
(2015) Doctor Philosophy — Associate Advisor
Other advisors:
(2011) Master Philosophy — Associate Advisor
Other advisors:
The essential and nonessential genes of Bovine herpesvirus-1
(2009) Doctor Philosophy — Associate Advisor
Other advisors:
Note for students: The possible research projects listed on this page may not be comprehensive or up to date. Always feel free to contact the staff for more information, and also with your own research ideas.
The development of strand-specific sequencing methodologies double-stranded DNA viral genomes
The herpesviruses are a large family of viruses that infected a broad range of hosts, such as mammals, reptiles, and molluscs. These viruses have a large double stranded DNA (dsDNA) genome. While the herpesviruses are considered to be genetically stable, variants with increased capacity to cause disease have emerged. The molecular basis underpinning the emergence of these virulent subtypes/genotypes for several herpesviruses of importance to veterinary medicine remains poorly understood. This project will utilise Oxford Nanopore sequencing technology to develop strategies to enable the strand-specific sequencing of herpesvirus genomes. The availability of strand-specific sequence data will be used to determine if the emergence of new genotypes of herpesviruses is a result of spontaneous mutation or the selection of existing genetic diversity within a viral isolate. This new knowledge will improve our understanding of this important virus family.
The application of synthetic biology to large DNA viral genomes
The capacity to manipulate the genomes of viruses in the laboratory remains dependent on their in vitro replication efficiency. This limitation has hindered our attempts better understand many of these viruses. This project will address this issue by applying long range PCR and DNA fragment assembly to construction copies of selected herpesviral genomes. The capacity of these genomes to facilitate rescue of infectious virus will also be determined. The availability of these infectious genome copies will aide in the development of improved diagnostics and control methods for these currently difficult to work with viruses.
The application of gene editing to improve animal resistance to infections
Gene editing technologies have enabled the rapid and specific modification of the genomes of complex organisms. Projects are available that will focus on the application of gene editing to increase the resilience of livestock cells to pathogens which reduce productivity. These projects will enable the candidate to gain experience in mammalian cell culture, transfection, growth and titration of viruses, molecular cloning, Western blotting, quantitative real-time PCR, genomic analyses, and fluorescence microscopy. These studies will improve our understanding of host/pathogen interactions towards the development of more resilient livestock.
Characterisation of bovine herpesvirus 2 genome
Bovine herpesvirus 2 (BoHV-2) is associated with mastitis in cattle. Of all of the herpesviruses which infect cattle, BoHV-2 is by far the most poorly characterised. This project will address this knowledge gap by using next generation sequencing and conventional PCR and sequencing technologies to completely sequence the BoHV-2 genome. The results of this project will enable the genome-wide comparisons of the BoHV-2 genome to other bovine and mammalian herpesviruses. These comparisons will improve our understanding of host/virus interactions at the molecular level for important viral properties such as tissue tropism and virulence.
Construction of bovine herpesvirus 5 infectious clone
Bovine herpesvirus 1 (BoHV-5) is a neurotrophic which is associated with severe and fatal disease in young cattle. Unlike bovine herpesvirus 1, BoHV-5 occurs sporadically in the world’s cattle population, except in South America. In this project, you will utilise genome edit tools, such as CRISPR/Cas9, to manipulate the BoHV-5 genome to construct an infectious clone of the virus. The results of this project will provide a better understanding of gene function in BoHV-5, particularly in respect to virulence. This information will improve our understand of how BoHV-5 interacts with its host at the molecular level and lead to the development of improved control strategies.