The University of Queensland

Fluid shear stress promotes embryonic stem cell pluripotency via interplay between β-catenin and vinculin in bioreactor culture

Nath, Suman C., Day, Bradley, Harper, Lane, Yee, Jeffrey, Hsu, Charlie Yu-Ming, Larijani, Leila, Rohani, Leili, Duan, Nicholas, Kallos, Michael S. and Rancourt, Derrick E. (2021). Fluid shear stress promotes embryonic stem cell pluripotency via interplay between β-catenin and vinculin in bioreactor culture. Stem Cells, 39 (9), 1166-1177. doi: 10.1002/stem.3382

Cytokine directed chondroblast trans-differentiation: JAK inhibition facilitates direct reprogramming of fibroblasts to chondroblasts

Cota, Perla, Helmi, Summer A., Hsu, Charlie and Rancourt, Derrick E. (2020). Cytokine directed chondroblast trans-differentiation: JAK inhibition facilitates direct reprogramming of fibroblasts to chondroblasts. Cells, 9 (1) 435, 191. doi: 10.3390/cells9010191

Using computational fluid dynamics (CFD) modeling to understand murine embryonic stem cell aggregate size and pluripotency distributions in stirred suspension bioreactors

Borys, Breanna S., Le, An, Roberts, Erin L., Dang, Tiffany, Rohani, Leili, Hsu, Charlie Yu-Ming, Wyma, Alexander A., Rancourt, Derrick E., Gates, Ian D. and Kallos, Michael S. (2019). Using computational fluid dynamics (CFD) modeling to understand murine embryonic stem cell aggregate size and pluripotency distributions in stirred suspension bioreactors. Journal of Biotechnology, 304, 16-27. doi: 10.1016/j.jbiotec.2019.08.002

An integrated approach toward the biomanufacturing of engineered cell therapy products in a stirred-suspension bioreactor

Hsu, Charlie Y. M., Walsh, Tylor, Borys, Breanna S., Kallos, Michael S. and Rancourt, Derrick E. (2018). An integrated approach toward the biomanufacturing of engineered cell therapy products in a stirred-suspension bioreactor. Molecular Therapy-Methods and Clinical Development, 9, 376-389. doi: 10.1016/j.omtm.2018.04.007

Lubricin/Proteoglycan 4 binds to and regulates the activity of toll-like receptors in vitro

Iqbal, S. M., Leonard, C., Regmi, S. C., De Rantere, D., Tailor, P., Ren, G., Ishida, H., Hsu, C. Y., Abubacker, S., Pang, D. S. J., Salo, P. T., Vogel, H. J., Hart, D. A., Waterhouse, C. C., Jay, G. D., Schmidt, T. A. and Krawetz, R. J. (2016). Lubricin/Proteoglycan 4 binds to and regulates the activity of toll-like receptors in vitro. Scientific Reports, 6 (1) 18910, 18910. doi: 10.1038/srep18910

Modification of human BMSC with nanoparticles of polymeric biomaterials and plasmid DNA for BMP-2 secretion

Wang, Yufu, Mostafa, Nesrine Z., Hsu, Charlie Y. M., Rose, Laura, Kucharki, Cezary, Yan, Jinglong, Jiang, Hongxing and Uludag, Hasan (2013). Modification of human BMSC with nanoparticles of polymeric biomaterials and plasmid DNA for BMP-2 secretion. Journal of Surgical Research, 183 (1), 8-17. doi: 10.1016/j.jss.2012.11.061

Cellular uptake pathways of lipid-modified cationic polymers in gene delivery to primary cells

Hsu, Charlie Y. M. and Uludag, Hasan (2012). Cellular uptake pathways of lipid-modified cationic polymers in gene delivery to primary cells. Biomaterials, 33 (31), 7834-7848. doi: 10.1016/j.biomaterials.2012.06.093

Effective down-regulation of Breast Cancer Resistance Protein (BCRP) by siRNA delivery using lipid-substituted aliphatic polymers

Aliabadi, Hamidreza Montazeri, Landry, Breanne, Mandipoor, Parvin, Hsu, Charlie Y. M. and Uludag, Hasan (2012). Effective down-regulation of Breast Cancer Resistance Protein (BCRP) by siRNA delivery using lipid-substituted aliphatic polymers. European Journal of Pharmaceutics and Biopharmaceutics, 81 (1), 33-42. doi: 10.1016/j.ejpb.2012.01.011

Nucleic-acid based gene therapeutics: delivery challenges and modular design of nonviral gene carriers and expression cassettes to overcome intracellular barriers for sustained targeted expression

Hsu, Charlie Yu Ming and Uludag, Hasan (2012). Nucleic-acid based gene therapeutics: delivery challenges and modular design of nonviral gene carriers and expression cassettes to overcome intracellular barriers for sustained targeted expression. Journal of Drug Targeting, 20 (4), 301-328. doi: 10.3109/1061186X.2012.655247

A simple and rapid nonviral approach to efficiently transfect primary tissue-derived cells using polyethylenimine

Hsu, Charlie Yu Ming and Uludag, Hasan (2012). A simple and rapid nonviral approach to efficiently transfect primary tissue-derived cells using polyethylenimine. Nature Protocols, 7 (5), 935-945. doi: 10.1038/nprot.2012.038

Improved transfection efficiency of an aliphatic lipid substituted 2 kDa polyethylenimine is attributed to enhanced nuclear association and uptake in rat bone marrow stromal cell

Hsu, Charlie Yu Ming, Hendzel, Michael and Uludag, Hasan (2011). Improved transfection efficiency of an aliphatic lipid substituted 2 kDa polyethylenimine is attributed to enhanced nuclear association and uptake in rat bone marrow stromal cell. Journal of Gene Medicine, 13 (1), 46-59. doi: 10.1002/jgm.1526

Erratum: Aliphatic lipid substitution on 2 kDa polyethylenimine improves plasmid delivery and transgene expression (Molecular Pharmaceutics (2009) 6:6 (1798-1815))

Neamnark, Artphop, Suwantong, Orawan, Remant Bahadur, K. C., Hsu, Charlie Y.M., Supaphol, Pitt and Uludaǧ, Hasan (2010). Erratum: Aliphatic lipid substitution on 2 kDa polyethylenimine improves plasmid delivery and transgene expression (Molecular Pharmaceutics (2009) 6:6 (1798-1815)). Molecular Pharmaceutics, 7 (2), 618-618. doi: 10.1021/mp1000263

Nonviral delivery of basic fibroblast growth factor gene to bone marrow stromal cells

Clements, Basak Acan, Hsu, Charlie Y. M., Kucharski, Cezary, Lin, Xiaoyue, Rose, Laura and Uludag, Hasan (2009). Nonviral delivery of basic fibroblast growth factor gene to bone marrow stromal cells. Clinical Orthopaedics and Related Research, 467 (12), 3129-3137. doi: 10.1007/s11999-009-0900-0

Aliphatic lipid substitution on 2 kDa polyethylenimine improves plasmid delivery and transgene expression

Neamnark, Artphop, Suwantong, Orawan, Bahadur, Remant K. C., Hsu, Charlie Y. M., Supaphol, Pitt and Uludag, Hasan (2009). Aliphatic lipid substitution on 2 kDa polyethylenimine improves plasmid delivery and transgene expression. Molecular Pharmaceutics, 6 (6), 1798-1815. doi: 10.1021/mp900074d

Further investigation of lipid-substituted poly(L-Lysine) polymers for transfection of human skin fibroblasts

Abbasi, Meysam, Uludag, Hasan, Incani, Vanessa, Hsu, Charlie Yu Ming and Jeffery, Andrea (2008). Further investigation of lipid-substituted poly(L-Lysine) polymers for transfection of human skin fibroblasts. Biomacromolecules, 9 (6), 1618-1630. doi: 10.1021/bm800132n

Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers

Hsu, Charlie Yu Ming and Uludag, Hasan (2008). Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers. BMC Biotechnology, 8, 1-15. doi: 10.1186/1472-6750-8-23

An optimized approach to non-viral transfection of brain tumor initiating cells derived from embryonal tumor with multilayered rosettes using cationic reagents

Hsu, Charlie Y., Chen, Myra J., Rancourt, Derrick E. and Chan, Jennifer A. (2016). An optimized approach to non-viral transfection of brain tumor initiating cells derived from embryonal tumor with multilayered rosettes using cationic reagents. 19th Annual Meeting of the American Society of Gene and Cell Therapy (ASGCT), Washington, DC, United States, May 4 - 7, 2016. Cambridge, MA, United States: Cell Press. doi: 10.1016/s1525-0016(16)33409-8

En route to non-viral genetic engineering: kinetics of DNA uptake and transgene expression following repeated transfection with multiple episomal plasmids in human primary fibroblast

Hsu, Charlie Y. and Rancourt, Derrick E. (2016). En route to non-viral genetic engineering: kinetics of DNA uptake and transgene expression following repeated transfection with multiple episomal plasmids in human primary fibroblast. 19th Annual Meeting of the American Society of Gene and Cell Therapy (ASGCT), Washington, DC, United States, May 4 - 7, 2016. Cambridge, MA, United States: Cell Press. doi: 10.1016/s1525-0016(16)33153-7

Non-viral transfection of primary human fibroblasts in suspension microcarrier culture

Hsu, Charlie Y. and Rancourt, Derrick E. (2016). Non-viral transfection of primary human fibroblasts in suspension microcarrier culture. 19th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT), Washington, DC, United States, May 4 - 7, 2016. Cambridge, MA, United States: Cell Press. doi: 10.1016/s1525-0016(16)33152-5

Reduced transgene persistence and trafficking to nuclear periphery are barriers to transfection in lipid substituted nonviral cationic polymer

Hsu, Charlie and Uludag, Hasan (2010). Reduced transgene persistence and trafficking to nuclear periphery are barriers to transfection in lipid substituted nonviral cationic polymer. 3rd International Cellular Delivery of Therapeutic Macromolecules (CDTM) Symposium, Cardiff, Wales, June 26 - 29, 2010. London, United Kingdom: Elsevier. doi: 10.1016/j.drudis.2010.09.419

Improved Plasmid DNA Delivery by Lipid-Substitution on 2 kDa Polyethylenimine (PEI)

Uludag, Hasan, Artphop, Neamnark, Suwantong, Orawan, Hsu, Charlie Y. M. and Supaphol, Pitt (2009). Improved Plasmid DNA Delivery by Lipid-Substitution on 2 kDa Polyethylenimine (PEI). American Society of Gene Therapy 12th Annual Meeting, San Diego, CA United States, 27- 30 May 2009. Cambridge, MA United States: Elsevier. doi: 10.1016/s1525-0016(16)39245-0

Persistence of Plasmid DNA Does Not Lead to Sustained Transgene Expression in PEI Mediated Transfection In Vitro: Implication of Transcriptional and Post-Transcriptional Silencing

Hsu, Charlie and Uludag, Hasan (2009). Persistence of Plasmid DNA Does Not Lead to Sustained Transgene Expression in PEI Mediated Transfection In Vitro: Implication of Transcriptional and Post-Transcriptional Silencing. American Society of Gene Therapy 12th Annual Meeting, San Diego, CA United States, 27- 30 May 2009. Cambridge, MA United States: Cell Press. doi: 10.1016/s1525-0016(16)39225-5

Transfection Mediated by Cationic Polymer In Vitro Is Enhanced by Blocking NF-kB Activation: Involvement of the Intracellular Innate Immune Response in Non-Viral Gene Delivery

Hsu, Charlie Y. M. and Uludag, Hasan (2009). Transfection Mediated by Cationic Polymer In Vitro Is Enhanced by Blocking NF-kB Activation: Involvement of the Intracellular Innate Immune Response in Non-Viral Gene Delivery. American Society of Gene Therapy 12th Annual Meeting, San Diego, CA United States, 27 - 30 May 2009. Cambridge, MA United States: Elsevier . doi: 10.1016/s1525-0016(16)38497-0

The effects DNA molecular weights and conformations on cell uptake and the binding interaction with cationic polymers used for non-viral gene delivery

Hsu, Charlie Y. -M., Kucharski, Cezary and Uludag, Hasan (2006). The effects DNA molecular weights and conformations on cell uptake and the binding interaction with cationic polymers used for non-viral gene delivery. American Society of Gene Therapy 9th Annual Meeting, Baltimore, MD, United States, May 31 - June 4 2006. Cambridge, MA, United States: Cell Press. doi: 10.1016/j.ymthe.2006.08.601