Dr. Andrés Gambini graduated with honors as a veterinarian at the University of Río Cuarto, Argentina, in 2008. After a period on an equine farm, he completed a Ph.D. program at the University of Buenos Aires (UBA) and taught in Animal Production Department. His Ph.D. focused on in vitro embryo production in horses. He produced the first cloned horses born in South America in 2010 and Australia in 2018. In 2015, he started a two years postdoc at the National Institute of Environmental Health Sciences (NIEHS) in the USA, where he investigated mechanisms governing embryonic genome activation in mammals. Andrés was invited as a professor at the University of Torino in Italy and the University of Cordoba in Spain. He continues his research in animal cloning, ICSI, and other reproductive biotechnologies. He recently reported the first cloned zebra embryos worldwide. He stood as Assistant Professor at the University of Buenos Aires, providing more than twelve years of lecturing, and mentoring undergraduate and postgraduate students in areas of animal physiology and reproduction. Currently, he is a senior lecturer in Animal Science, School of Agriculture and Food Sciences at University of Queensland, Australia.
Assisted Reproductive techniques and particularly in vitro embryo production have revolutionized the way we are now thinking about animal production and conservation of biodiversity. The future of the animal industry for food, transportation, biomedicine and sports is now relaying on these new different biotechnologies.
Journal Article: Selection and conservation of gametes and embryos for improving assisted reproductive technologies
Gambini, Andrés, Ortiz, Isabel, Hidalgo, Manuel and Dorado, Jesús (2023). Selection and conservation of gametes and embryos for improving assisted reproductive technologies. Animals, 13 (17) 2761, 2761. doi: 10.3390/ani13172761
Journal Article: Effect of transvaginal oocyte aspiration on progesterone concentrations and estrous cycle length in domestic jennies
Bragulat, Ana Flores, Alonso, Carolina, Castañeira, Catalina, Rodriguez, Ayelen, Zeledon, Jose M, Losinno, Luis and Gambini, Andres (2023). Effect of transvaginal oocyte aspiration on progesterone concentrations and estrous cycle length in domestic jennies. Journal of Equine Veterinary Science, 125 104649, 104649. doi: 10.1016/j.jevs.2023.104649
Journal Article: Sperm vitrification supports in vitro blastocyst development after ICSI in horses
Ortiz, Isabel, Dorado, Jesus, Hidalgo, Manuel and Gambini, Andrés (2023). Sperm vitrification supports in vitro blastocyst development after ICSI in horses. Journal of Equine Veterinary Science, 125 104669, 104669. doi: 10.1016/j.jevs.2023.104669
Novel strategies for sperm conservation in Koalas and Horses
Let's explore new strategies to cryopreserve sperm cells as freeze drying (lyophilization) or microwave drying, and to make embryos in the laboratory! Contact me for more information.
New methods for oocyte assisted activation
Contact me for more information.
Assisted Reproduction Technologies. In vitro embryo culture, ICSI, Cloning, IVF, in vitro maturation, Vitrification, Sperm.
Contact me for more information.
Clonación y transferencia nuclear aplicada a la reproducción
Gambini, Andres and Briski, Olinda (2022). Clonación y transferencia nuclear aplicada a la reproducción. Embriologia clinica: presente y futuro. (pp. 452-490) edited by Iván Anduaga Marchetti, Alicia Pené and Gustavo Martínez. Cordoba, Argentina: Universidad Nacional de Cordoba.
The relevance of the donor cell for horse cloning
Gambini, Andres and Salamone, Daniel (2019). The relevance of the donor cell for horse cloning. Equine assisted reproduction. (pp. 77-89) edited by L. Losinno, R. Sanchez and T. Stout. Colombia: FAVE Editorial.
Selection and conservation of gametes and embryos for improving assisted reproductive technologies
Gambini, Andrés, Ortiz, Isabel, Hidalgo, Manuel and Dorado, Jesús (2023). Selection and conservation of gametes and embryos for improving assisted reproductive technologies. Animals, 13 (17) 2761, 2761. doi: 10.3390/ani13172761
Bragulat, Ana Flores, Alonso, Carolina, Castañeira, Catalina, Rodriguez, Ayelen, Zeledon, Jose M, Losinno, Luis and Gambini, Andres (2023). Effect of transvaginal oocyte aspiration on progesterone concentrations and estrous cycle length in domestic jennies. Journal of Equine Veterinary Science, 125 104649, 104649. doi: 10.1016/j.jevs.2023.104649
Sperm vitrification supports in vitro blastocyst development after ICSI in horses
Ortiz, Isabel, Dorado, Jesus, Hidalgo, Manuel and Gambini, Andrés (2023). Sperm vitrification supports in vitro blastocyst development after ICSI in horses. Journal of Equine Veterinary Science, 125 104669, 104669. doi: 10.1016/j.jevs.2023.104669
Plaza, Jessica P, Arraztoa, Claudia C, Gambini, Andres, Gambarotta, Mariana, Miragaya, Marcelo H and Neild, Deborah M (2023). Ultrasonographic and Doppler characteristics of corpus luteum luteogenesis in Remonta Argentino jennies. Journal of Equine Veterinary Science, 125 104738, 104738. doi: 10.1016/j.jevs.2023.104738
Cabeza, Juan P. and Gambini, Andrés (2023). Advancements and Challenges in In Vitro Reproductive Technologies for the Conservation of Equine Species. Theriogenology Wild, 2 100036, 100036. doi: 10.1016/j.therwi.2023.100036
Helping endangered donkeys and zebras from the laboratory
Gambini, Andrés and Duncan, Nicole (2023). Helping endangered donkeys and zebras from the laboratory. Aether. doi: 10.58214/uq2702
Flores Bragulat, Ana P., Ortiz, Isabel, Catalán, Jaime, Dorado, Jesus, Hidalgo, Manuel, Losinno, Luis, Dordas-Perpinyà, Marta, Yánez-Ortiz, Iván, Martínez-Rodero, Iris, Miró, Jordi and Gambini, Andrés (2023). Time-lapse imaging and developmental competence of donkey eggs after ICSI: Effect of preovulatory follicular fluid during oocyte in vitro maturation. Theriogenology, 195, 199-208. doi: 10.1016/j.theriogenology.2022.10.030
Preimplantation developmental competence of bovine and porcine oocytes activated by zinc chelation
Cabeza, Juan P., Cámera, Juan, Briski, Olinda, Felipe, Minerva Yauri, Salamone, Daniel F. and Gambini, Andrés (2022). Preimplantation developmental competence of bovine and porcine oocytes activated by zinc chelation. Animals, 12 (24) 3560, 1-12. doi: 10.3390/ani12243560
140 Assisted activation of intracytoplasmic sperm injection porcine zygotes using zinc chelation
Briski, O., Gambini, A., Cabeza, J., Allegroni, F., Ratner, L., Fernández-Martin, R. and Salamone, D. (2022). 140 Assisted activation of intracytoplasmic sperm injection porcine zygotes using zinc chelation. Reproduction, Fertility and Development, 35 (2), 198-198. doi: 10.1071/rdv35n2ab140
In vitro production of zebra cloned embryos
Gambini, A., Duque Rodriguez, M., Briski, O., Bragulat, F., Demergasi, N., Losinno, L. and Salamone, D. F. (2022). In vitro production of zebra cloned embryos. Journal of Equine Veterinary Science, 113 103994. doi: 10.1016/j.jevs.2022.103994
Phospholipase C zeta characterization and egg activation ability of donkey sperm
Arroyo-Salvo, C., Camera, J., Cabeza, J. P., Perez Martinez, S., Hibling, A., Plaza, J., Miragaya, M., Salamone, D. F. and Gambini, A. (2022). Phospholipase C zeta characterization and egg activation ability of donkey sperm. Journal of Equine Veterinary Science, 113 103995. doi: 10.1016/j.jevs.2022.103995
The efficiency of transvaginal oocyte aspiration in Remonta Argentino jennies
Ferrante, A. A., Arraztoa, C. C., Plaza, J. P., Gambini, A., Baca Castex, C., Rodriguez, M. B., Brisky, O., Bruno, S., Olivieri, G., Miragaya, M. H. and Neild, D. M. (2022). The efficiency of transvaginal oocyte aspiration in Remonta Argentino jennies. Journal of Equine Veterinary Science, 113 103996. doi: 10.1016/j.jevs.2022.103996
State of the art of nuclear transfer technologies for assisting mammalian reproduction
Gambini, Andres, Briski, Olinda and Canel, Natalia Gabriela (2022). State of the art of nuclear transfer technologies for assisting mammalian reproduction. Molecular Reproduction and Development, 89 (5-6), 230-242. doi: 10.1002/mrd.23615
Aggregation of Leopardus geoffroyi hybrid embryos with domestic cat tetraploid blastomeres
Duque Rodriguez, Matteo, Gambini, Andres, Ratner, Laura D., Sestelo, Adrian J., Briski, Olinda, Gutnisky, Cynthia, Rulli, Susana B., Fernandez Martin, Rafael, Cetica, Pablo and Salamone, Daniel F. (2021). Aggregation of Leopardus geoffroyi hybrid embryos with domestic cat tetraploid blastomeres. Reproduction, 161 (5), 539-548. doi: 10.1530/REP-20-0589
Gambini, Andres, Duque Rodriguez, Matteo, Belen Rodriguez, Maria, Briski, Olinda, Flores Bragulat, Ana P., Demergassi, Natalia, Losinno, Luis and Salamone, Daniel F. (2020). Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern. PLoS One, 15 (9) e0238948, e0238948. doi: 10.1371/journal.pone.0238948
Gambini, Andres, Stein, Paula, Savy, Virginia, Grow, Edward J., Papas, Brian N., Zhang, Yingpei, Kenan, Anna C., Padilla-Banks, Elizabeth, Cairns, Bradley R. and Williams, Carmen J. (2020). Developmentally programmed tankyrase activity upregulates beta-catenin and licenses progression of embryonic genome activation. Developmental Cell, 53 (5), 545-560. doi: 10.1016/j.devcel.2020.04.018
Targeting epigenetic nuclear reprogramming in aggregated cloned equine embryos
Teixeira, Thiago V. Damasceno, Fry, Richard C., McKinnon, Angus, Fry, Kerri L., Kelly, Jennifer M., Verma, Paul J., Burden, Chelsie, Salamone, Daniel F. and Gambini, Andres (2019). Targeting epigenetic nuclear reprogramming in aggregated cloned equine embryos. Reproduction Fertility and Development, 31 (12), 1885-1893. doi: 10.1071/RD19239
Belen Rodriguez, Maria, Gambini, Andres, Clerico, Gabriel, Eugenia Ynsaurralde-Rivolta, Amada, Briski, Olinda, Largel, Hernan, Sansinena, Marina and Salamone, Daniel F. (2019). Time of first polar body extrusion affects the developmental competence of equine oocytes after intracytoplasmic sperm injection. Reproduction Fertility and Development, 31 (12), 1805-1811. doi: 10.1071/RD19248
Miao, Yi-Liang, Gambini, Andres, Zhang, Yingpei, Padilla-Banks, Elizabeth, Jefferson, Wendy N., Bernhardt, Miranda L., Huang, Weichun, Li, Leping and Williams, Carmen J. (2018). Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse. Biology of Reproduction, 98 (4), 449-464. doi: 10.1093/biolre/ioy004
Gambini, Andres, De Stefano, Adrian, Jarazo, Javier, Buemo, Carla, Karlanian, Florencia and Felipe Salamone, Daniel (2016). Embryo aggregation does not improve the development of interspecies somatic cell nuclear transfer embryos in the horse. Theriogenology, 86 (4), 1081-1091. doi: 10.1016/j.theriogenology.2016.03.040
LUTs of blastocyst nuclei cell for quantification
Gambini, Andrés and Williams, Carmen J. (2016). LUTs of blastocyst nuclei cell for quantification. Molecular Reproduction and Development, 83 (7), 575-575. doi: 10.1002/mrd.22677
Embryo aggregation in pig improves cloning efficiency and embryo quality
Paola Buemo, Carla, Gambini, Andres, Natalia Moro, Lucia, Ines Hiriart, Maria, Fernandez-Martin, Rafael, Collas, Philippe and Felipe Salamone, Daniel (2016). Embryo aggregation in pig improves cloning efficiency and embryo quality. PLoS One, 11 (2) e0146390. doi: 10.1371/journal.pone.0146390
Gambini, Andres, De Stefano, Adrian, Jimena Bevacqua, Romina, Karlanian, Florencia and Felipe Salamone, Daniel (2014). The aggregation of four reconstructed zygotes is the limit to improve the developmental competence of cloned equine embryos. PLoS One, 9 (11) e110998. doi: 10.1371/journal.pone.0110998
Andrés, G., Javier, J., Florencia, K., De Stéfano, A. and Salamone, D. F. (2014). Effect of collection-maturation interval time and pregnancy status of donor mares on oocyte developmental competence in horse cloning. Journal of Animal Science, 92 (2), 561-567. doi: 10.2527/jas.2013-7026
Salamone, D., Bevacqua, R., Gambini, A., Canel, N., Hiriart, M. I., Moro, L., Buemo, C. and Gibbons, A. (2014). New micromanipulation technologies in domestic species Nuevas tecnologías de micromanipulación en especies domésticas. Taurus, 16 (62), 66-68.
Equine cloning: in vitro and in vivo development of aggregated embryos
Gambini, Andres, Jarazo, Javier, Olivera, Ramiro and Salamone, Daniel F. (2012). Equine cloning: in vitro and in vivo development of aggregated embryos. Biology of Reproduction, 87 (1) 15. doi: 10.1095/biolreprod.112.098855
Recent advances in micromanipulation and transgenesis in domestic mammals
Salamone, Daniel, Bevacqua, Romina, Bonnet, Federico Pereyra, Gambini, Andrés, Canel, Natalia, Inés Hiriart, M., Vichera, Gabriel, Moro, Lucia and Jarazo, Javier (2011). Recent advances in micromanipulation and transgenesis in domestic mammals. Acta Scientiae Veterinariae, 39 (SUPPL. 1).
Development and quality of embryos generated by zinc chelation of bovine eggs
Camera , Juan , Cabeza , Juan Pedro , Yauri Felipe, Minerva , Briski , Olinda , Salamone, Daniel and Gambini , Andrés (2022). Development and quality of embryos generated by zinc chelation of bovine eggs. 35th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Brazil, 18-23 August 2022. Belo Horizonte, Brazil: Colegio Brasileiro de Reproducao Animal.
Camera, Juan, Cabeza, Juan Pedro, Yauri Felipe, Minerva, Briski, Olinda, Salamone, Daniel and Gambini, Andrés (2022). Developmental rates and quality of blastocysts generated by zinc chelation and intracytoplasmic calcium rise of porcine eggs. 35th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Brazil, 18-22 August 2022. Horizonte, Brazil: Colegio Brasileiro de Reproducao Animal.
In vitro development of mule ICSI embryos
Cogollo Villareal , Marïa Y. , Niño Vargas , Andrea , Restrepo, Juan F. , Briski , Olinda , Hernández Grajales, J.X and Gambini, Andres (2022). In vitro development of mule ICSI embryos. 35th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Brazil, 18-23 August 2022. Belo Horizonte, Brazil: Colegio Brasileiro de Reproducao Animal.
Gambini, A., Rodriguez, M. B., Briski, O., Flores Bragulat, A., Demergasi, N., Losinno, L. and Salamone, D. F. (2020). Ability of the zebra sperm to induce pig and horse oocyte activation and in vitro preimplantation embryo development after ICSI. 10th International Symposium on Equine Embryo Transfer, Pisa, Italy, 2-5 July 2020. Philadelphia, PA USA: Elsevier. doi: 10.1016/j.jevs.2020.103052
A journey through horse cloning
Gambini, Andres and Maserati, Marc (2018). A journey through horse cloning. Annual Conference of the International Embryo Technology Society (IETS), Bangkok, Thailand, 13-16 January 2018. Clayton, VIC Australia: CSIRO. doi: 10.1071/RD17374
Gambini, A., Jarazo, J., De Stefano, A., Karlaninan, F. and Salamone, D. (2013). EFFECT OF CULTURE AT LOW OR ATMOSPHERIC OXYGEN TENSION IN SOMATIC DONOR CELLS FOR HORSE NUCLEAR TRANSFER. COLLINGWOOD: CSIRO PUBLISHING. doi: 10.1071/RDv25n1Ab35
Jarazo, J., Gambini, A., Muredas, L., Fernandez-Martin, R. and Salamone, D. (2013). HORSE EMBRYO BIOPSY: EFFECT ON PREGNANCY RATES AND SUCCESSFUL SEX DETERMINATION DEPENDING ON THE SIZE OF THE EMBRYO. COLLINGWOOD: CSIRO PUBLISHING. doi: 10.1071/RDv25n1Ab151
Outgrowths and capsule derived from aggregated cloned equine embryos
Gambini, A., Jarazo, J., De Stefano, A., Karlanian, F. and Salamone, D. (2012). Outgrowths and capsule derived from aggregated cloned equine embryos. 17th International Congress on Animal Reproduction (ICAR), Vancouver Canada, Jul 29-Aug 02, 2012. HOBOKEN: WILEY-BLACKWELL.
EQUINE CLONING AND EMBRYO AGGREGATION: EFFECT OF BOVINE, PORCINE, FELINE AND EQUINE OOPLAST
Gambini, A., Jarazo, J., De Stefano, A., Karlanian, F. and Salamone, D. (2012). EQUINE CLONING AND EMBRYO AGGREGATION: EFFECT OF BOVINE, PORCINE, FELINE AND EQUINE OOPLAST. COLLINGWOOD: CSIRO PUBLISHING. doi: 10.1071/RDv24n1Ab14
Jarazo, J., Gambini, A., De Stefano, A., Muredas, L., Oriol, J. G. and Salamone, D. (2012). EQUINE EMBRYO IN VITRO DEVELOPMENT AFTER INTRACYTOPLASMIC SPERM INJECTION FOLLOWED BY CHEMICAL ACTIVATION. COLLINGWOOD: CSIRO PUBLISHING. doi: 10.1071/RDv24n1Ab203
AGGREGATION OF CLONED EQUINE EMBRYOS: IMPROVEMENT OF IN VITRO AND IN VIVO DEVELOPMENT
Gambini, A., Jarazo, J., Olivera, R., Karlanian, F. and Salamone, D. F. (2011). AGGREGATION OF CLONED EQUINE EMBRYOS: IMPROVEMENT OF IN VITRO AND IN VIVO DEVELOPMENT. COLLINGWOOD: CSIRO PUBLISHING. doi: 10.1071/RDv23n1Ab123
Gambini, A., Jarazo, J., Olivera, R. and Salamone, D. (2010). EFFECT OF THE TIME INTERVAL BETWEEN OVARY COLLECTION AND OOCYTE IN VITRO MATURATION ON EQUINE CLONED EMBRYO DEVELOPMENT. COLLINGWOOD: CSIRO PUBLISHING. doi: 10.1071/RDv22n1Ab53
1. Methods for Artificial Oocyte Activation
Bernhardt, Miranda L., Williams, Carmen J., Gambini, Andres and Mehlmann, Lisa M. (2016). 1. Methods for Artificial Oocyte Activation.
Note for students: The possible research projects listed on this page may not be comprehensive or up to date. Always feel free to contact the staff for more information, and also with your own research ideas.
Novel strategies for sperm conservation in Koalas and Horses
Let's explore new strategies to cryopreserve sperm cells as freeze drying (lyophilization) or microwave drying, and to make embryos in the laboratory! Contact me for more information.
New methods for oocyte assisted activation
Contact me for more information.
Assisted Reproduction Technologies. In vitro embryo culture, ICSI, Cloning, IVF, in vitro maturation, Vitrification, Sperm.
Contact me for more information.