The University of Queensland

Preface

Botella, Jose R. and Botella, Miguel A. (2016). Preface. Plant Signal Transduction Methods and Protocols. (pp. VII-X) New York, NY, United States: Humana Press. doi: 10.1007/978-1-4939-3115-6

Simplified assays for evaluation of resistance to Alternaria brassicicola and Turnip Mosaic Virus

Trusov, Yuri, Dietzgen, Ralf G., Maruta, Natsumi and Botella, Jose R. (2016). Simplified assays for evaluation of resistance to Alternaria brassicicola and Turnip Mosaic Virus. In Botella, JR and Botella, MA (Ed.), Plant Signal Transduction (pp. 219-228) New York, NY, United States: Humana Press. doi:10.1007/978-1-4939-3115-6_18

Yeast three-hybrid system for the detection of protein-protein interactions

Maruta, Natsumi, Trusov, Yuri and Botella, Jose R. (2016). Yeast three-hybrid system for the detection of protein-protein interactions. In Jose R. Botella and Miguel A. Botella (Ed.), Plant signal transduction Second edition ed. (pp. 145-154) New York, NY, United States: Humana Press. doi:10.1007/978-1-4939-3115-6_12

Fusarium oxysporum infection assays in Arabidopsis

Trusov, Yuri, Chakravorty, David and Botella, Jose Ramon (2013). Fusarium oxysporum infection assays in Arabidopsis. G protein-coupled receptor signaling in plants: methods and protocols. (pp. 67-72) edited by Mark P. Running. New York, United States: Humana Press. doi: 10.1007/978-1-62703-532-3_7

G Proteins and Plant Innate Immunity

Trusov, Yuri, Jorda, Lucia, Molina, Antonio and Botella, Jose Ramon (2010). G Proteins and Plant Innate Immunity. Integrated G Proteins Signaling in Plants. (pp. 221-250) edited by Shaul Yalovsky, Frantisek Baluska and Alan Jones. Netherlands: Springer. doi: 10.1007/978-3-642-03524-1

Genomics of Pineapple, Crowning the King of Tropical Fruits

Botella, J.R. and Smith, M. (2008). Genomics of Pineapple, Crowning the King of Tropical Fruits. Plant Genetics/Genomics: Genomics of Tropical Crop Plants. (pp. 441-451) edited by Moore, P.H. and Ming, R.. United States: Springer.

RNA Interference

Brosnan, C.A., McCallum,E.J., Botella, J.R. and Carroll, B.J. (2008). RNA Interference. The Handbook of Plant Functional Genomics: Concepts and Protocols. (pp. 209-225) edited by Kahl, G. and Meksem, K.. Germany: Wiley - Blackwell. doi: 10.1002/9783527622542.ch10

Bromeliaceae

Smith, M. K., Ko, H. L., Sanewski, G. M. and Botella, J. R. (2005). Bromeliaceae. Biotechnology of Fruit and Nut Crops. (pp. 157-172) edited by R. E. Litz. Cambridge, USA: CABI Publishing.

Biotechnology and reduced spoilage

Botella, J. R. (2003). Biotechnology and reduced spoilage. Food Preservation Techniques. (pp. 243-262) edited by Peter Zeuthen and Leif Bogh-Sorensen. Cambridge, United Kingdom: Woodhead Publishing Limited. doi: 10.1016/B978-1-85573-530-9.50017-6

Genes selected for their role in modifying post-harvest life

Botella, J. R. (2002). Genes selected for their role in modifying post-harvest life. Fruit and Vegetable Biotechnology. (pp. 136-151) edited by Victoriano Valpuesta. Cambridge, England: Woodhead Publishing Ltd. doi: 10.1533/9781855736412.1.136

PDC1 is activated by ABF4 and inhibits seed germination by promoting ROS accumulation in Arabidopsis

Li, Liangliang, Li, Linxiao, Cui, Shenghui, Qian, Dongdong, Lyu, Shijie, Liu, Wencheng, Botella, José Ramón, Li, Huilin, Burritt, David J., Tran, Lam-Son Phan, Li, Weiqiang and Zhang, Yu (2023). PDC1 is activated by ABF4 and inhibits seed germination by promoting ROS accumulation in Arabidopsis. Environmental and Experimental Botany, 206 105188. doi: 10.1016/j.envexpbot.2022.105188

Overexpression of AHL9 accelerates leaf senescence in Arabidopsis thaliana

Zhou, Yusen, Zhang, Xiaomin, Chen, Jing, Guo, Xiaopeng, Wang, Hongyan, Zhen, Weibo, Zhang, Junli, Hu, Zhubing, Zhang, Xuebing, Botella, José Ramón, Ito, Toshiro and Guo, Siyi (2022). Overexpression of AHL9 accelerates leaf senescence in Arabidopsis thaliana. BMC Plant Biology, 22 (1) 248, 1-12. doi: 10.1186/s12870-022-03622-9

Insights into the molecular mechanisms of CRISPR/Cas9-mediated gene targeting at multiple loci in Arabidopsis

Zhang, Zhengjing, Zeng, Wenjie, Zhang, Wenxin, Li, Jing, Kong, Dali, Zhang, Lei, Wang, Rui, Peng, Fangnan, Kong, Zhe, Ke, Yongping, Zhang, Heng, Kim, Chanhong, Zhang, Huiming, Botella, Jose Ramón, Zhu, Jian-Kang and Miki, Daisuke (2022). Insights into the molecular mechanisms of CRISPR/Cas9-mediated gene targeting at multiple loci in Arabidopsis. Plant Physiology, 190 (4), 2203-2216. doi: 10.1093/plphys/kiac431

Point-of-care DNA amplification for disease diagnosis and management

Botella, José R. (2022). Point-of-care DNA amplification for disease diagnosis and management. Annual Review of Phytopathology, 60 (1), 1-20. doi: 10.1146/annurev-phyto-021621-115027

Are point of management assays relevant for food safety in the poultry industries?

Templeton, J. M., Botella, J. R. and Blackall, P. J. (2022). Are point of management assays relevant for food safety in the poultry industries?. Microbiology Australia, 43 (2), 67-70. doi: 10.1071/MA22025

Expanding the editing window of cytidine base editors with the Rad51 DNA-binding domain in rice

Wei, Chunjie, Liu, Hao, Wang, Wenwen, Luo, Pengyu, Chen, Qiuling, Li, Rou, Wang, Chong, Botella, José Ramón and Zhang, Hui (2022). Expanding the editing window of cytidine base editors with the Rad51 DNA-binding domain in rice. Frontiers in Plant Science, 13 865848, 1-9. doi: 10.3389/fpls.2022.865848

Function of protein kinases in leaf senescence of plants

Yang, Fengbo, Miao, Yuchen, Liu, Yuyue, Botella, Jose R., Li, Weiqiang, Li, Kun and Song, Chun-Peng (2022). Function of protein kinases in leaf senescence of plants. Frontiers in Plant Science, 13 864215, 1-16. doi: 10.3389/fpls.2022.864215

Heterotrimeric G protein signaling in abiotic stress

Wang, Yijie and Botella, Jose Ramón (2022). Heterotrimeric G protein signaling in abiotic stress. Plants, 11 (7) 876. doi: 10.3390/plants11070876

The high chlorophyll fluorescence 244 (HCF244) Is potentially involved in glutathione peroxidase 7-regulated high light stress in Arabidopsis thaliana

Li, Kun, Jia, Qianyun, Guo, Jinggong, Zhu, Zhijuan, Shao, Mengying, Wang, Jin, Li, Weiqiang, Dai, Jie, Guo, Minghui, Li, Rui, Song, Jieqiong, Yang, Fengbo, Du, Jingyi, Botella, Jose R., Song, Chun-Peng and Miao, Yuchen (2022). The high chlorophyll fluorescence 244 (HCF244) Is potentially involved in glutathione peroxidase 7-regulated high light stress in Arabidopsis thaliana. Environmental and Experimental Botany, 195 104767, 104767. doi: 10.1016/j.envexpbot.2021.104767

Abscisic acid-induced cytoplasmic translocation of constitutive photomorphogenic 1 enhances reactive oxygen species accumulation through the HY5-ABI5 pathway to modulate seed germination

Chen, Qing‐Bin, Wang, Wen‐Jing, Zhang, Yue, Zhan, Qi‐Di, Liu, Kang, Botella, José Ramón, Bai, Ling and Song, Chun‐Peng (2022). Abscisic acid-induced cytoplasmic translocation of constitutive photomorphogenic 1 enhances reactive oxygen species accumulation through the HY5-ABI5 pathway to modulate seed germination. Plant, Cell and Environment, 45 (5), 1474-1489. doi: 10.1111/pce.14298

Non-GM genome editing approaches in crops

Gong, Zheng, Cheng, Ming and Botella, Jose R. (2021). Non-GM genome editing approaches in crops. Frontiers in Genome Editing, 3, 817279. doi: 10.3389/fgeed.2021.817279

Endogenous U6 promoters improve CRISPR/Cas9 editing efficiencies in Sorghum bicolor and show potential for applications in other cereals

Massel, Karen, Lam, Yasmine, Hintzsche, Jessica, Lester, Nicholas, Botella, Jose R. and Godwin, Ian D. (2021). Endogenous U6 promoters improve CRISPR/Cas9 editing efficiencies in Sorghum bicolor and show potential for applications in other cereals. Plant Cell Reports, 41 (2), 489-492. doi: 10.1007/s00299-021-02816-z

Heterotrimeric G proteins in plants: canonical and atypical Gα subunits

Maruta, Natsumi, Trusov, Yuri, Jones, Alan M. and Botella, Jose R. (2021). Heterotrimeric G proteins in plants: canonical and atypical Gα subunits. International Journal of Molecular Sciences, 22 (21) 11841. doi: 10.3390/ijms222111841

Tomato and cotton G protein beta subunit mutants display constitutive autoimmune responses

Ninh, Thi Thao, Gao, Wei, Trusov, Yuri, Zhao, Jing‐Ruo, Long, Lu, Song, Chun‐Peng and Botella, Jose Ramon (2021). Tomato and cotton G protein beta subunit mutants display constitutive autoimmune responses. Plant Direct, 5 (11) e359, e359. doi: 10.1002/pld3.359

Manipulating assimilate availability provides insight into the genes controlling grain size in sorghum

Tao, Yongfu, Trusov, Yuri, Zhao, Xianrong, Wang, Xuemin, Cruickshank, Alan W., Hunt, Colleen, van Oosterom, Erik J., Hathorn, Adrian, Liu, Guoquan, Ian, Godwin D., Botella, Jose R., Mace, Emma S. and Jordan, David R. (2021). Manipulating assimilate availability provides insight into the genes controlling grain size in sorghum. The Plant Journal, 108 (1) tpj.15437, 1-13. doi: 10.1111/tpj.15437

GTP binding by Arabidopsis extra-large G protein 2 is not essential for its functions

Maruta, Natsumi, Trusov, Yuri, Urano, Daisuke, Chakravorty, David, Assmann, Sarah M., Jones, Alan M. and Botella, Jose R. (2021). GTP binding by Arabidopsis extra-large G protein 2 is not essential for its functions. Plant Physiology, 186 (2), 1-14. doi: 10.1093/plphys/kiab119

Expanding the target range of base editing in plants without loss of efficiency by blocking RNA-silencing

Mao, Yanfei, Wang, Mugui, Zhao, Yuhang, Huang, Boyu, Wu, Qingbing, Zheng, Qijie, Botella, Jose Ramon and Zhu, Jian-Kang (2021). Expanding the target range of base editing in plants without loss of efficiency by blocking RNA-silencing. Plant Biotechnology Journal, 19 (12), 2389-2391. doi: 10.1111/pbi.13712

Genome editing for plant research and crop improvement

Zhan, Xiangqiang, Lu, Yuming, Zhu, Jian-Kang and Botella, Jose Ramon (2021). Genome editing for plant research and crop improvement. Journal of Integrative Plant Biology, 63 (1), 3-33. doi: 10.1111/jipb.13063

COP1 promotes ABA‐induced stomatal closure by modulating the abundance of ABI/HAB and AHG3 phosphatases

Chen, Qingbin, Bai, Ling, Wang, Wenjing, Shi, Huazhong, Botella, José Ramón, Zhan, Qidi, Liu, Kang, Yang, Hong‐Quan and Song, Chun‐Peng (2020). COP1 promotes ABA‐induced stomatal closure by modulating the abundance of ABI/HAB and AHG3 phosphatases. New Phytologist, 229 (4) nph.17001, 2035-2049. doi: 10.1111/nph.17001

Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks

Mason, Michael G. and Botella, José R. (2020). Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks. Nature Protocols, 15 (11), 3663-3677. doi: 10.1038/s41596-020-0392-7

Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics

Zou, Yiping, Mason, Michael Glenn and Botella, Jose Ramon (2020). Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics. PLoS One, 15 (6) e0235216, e0235216. doi: 10.1371/journal.pone.0235216

ABC1K10a, an atypical kinase, functions in plant salt stress tolerance

Qin, Xiaohui, Duan, Zhikun, Zheng, Yuan, Liu, Wen-Cheng, Guo, Siyi, Botella, José Ramón and Song, Chun-Peng (2020). ABC1K10a, an atypical kinase, functions in plant salt stress tolerance. BMC Plant Biology, 20 (1) 270, 270. doi: 10.1186/s12870-020-02467-4

The gland localized CGP1 controls gland pigmentation and gossypol accumulation in cotton

Gao, Wei, Xu, Fu‐Chun, Long, Lu, Li, Yang, Zhang, Jun‐Li, Chong, Leelyn, Botella, Jose Ramon and Song, Chun‐Peng (2020). The gland localized CGP1 controls gland pigmentation and gossypol accumulation in cotton. Plant Biotechnology Journal, 18 (7) pbi.13323, 1-12. doi: 10.1111/pbi.13323

An easy‐to‐perform, culture‐free Campylobacter point‐of‐management assay for processing plant applications

Mason, M.G., Blackall, P.J., Botella, J.R. and Templeton, J.M. (2019). An easy‐to‐perform, culture‐free Campylobacter point‐of‐management assay for processing plant applications. Journal of Applied Microbiology, 128 (3) jam.14509, 620-629. doi: 10.1111/jam.14509

Nucleotide exchange–dependent and nucleotide exchange–independent functions of plant heterotrimeric GTP-binding proteins

Maruta, Natsumi, Trusov, Yuri, Chakravorty, David, Urano, Daisuke, Assmann, Sarah M. and Botella, Jose R. (2019). Nucleotide exchange–dependent and nucleotide exchange–independent functions of plant heterotrimeric GTP-binding proteins. Science Signaling, 12 (606) eaav9526, eaav9526. doi: 10.1126/scisignal.aav9526

A simple, robust and equipment-free DNA amplification readout in less than 30 seconds

Mason, Michael Glenn and Botella, José Ramón (2019). A simple, robust and equipment-free DNA amplification readout in less than 30 seconds. RSC Advances, 9 (42), 24440-24450. doi: 10.1039/c9ra04725e

Perspectives on the Application of Genome-Editing Technologies in Crop Breeding

Hua, Kai, Zhang, Jinshan, Botella, Jose Ramon, Ma, Changle, Kong, Fanjiang, Liu, Baohui and Zhu, Jian-Kang (2019). Perspectives on the Application of Genome-Editing Technologies in Crop Breeding. Molecular Plant, 12 (8), 1047-1059. doi: 10.1016/j.molp.2019.06.009

Beyond light: insights into the role of constitutively photomorphogenic1 in plant hormonal signaling

Wang, Wenjing, Chen, Qingbin, Botella, Jose Ramon and Guo, Siyi (2019). Beyond light: insights into the role of constitutively photomorphogenic1 in plant hormonal signaling. Frontiers in Plant Science, 10 557, 557. doi: 10.3389/fpls.2019.00557

Gene editing in plants: Progress and challenges

Mao, Yanfei, Botella, Jose Ramon, Liu, Yaoguang and Zhu, Jian-Kang (2019). Gene editing in plants: Progress and challenges. National Science Review, 6 (3), 421-437. doi: 10.1093/nsr/nwz005

Biosynthesis of DHGA12 and its roles in Arabidopsis seedling establishment

Liu, Hao, Guo, Siyi, Lu, Minghua, Zhang, Yu, Li, Junhua, Wang, Wei, Wang, Pengtao, Zhang, Junli, Hu, Zhubing, Li, Liangliang, Si, Lingyu, Zhang, Jie, Qi, Qi, Jiang, Xiangning, Botella, José Ramón, Wang, Hua and Song, Chun-Peng (2019). Biosynthesis of DHGA12 and its roles in Arabidopsis seedling establishment. Nature Communications, 10 (1) 1768, 1768. doi: 10.1038/s41467-019-09467-5

Now for the hard ones: is there a limit on CRISPR genome editing in crops?

Botella, Jose R. (2019). Now for the hard ones: is there a limit on CRISPR genome editing in crops?. Journal of Experimental Botany, 70 (3), 734-737. doi: 10.1093/jxb/erz007

A highly efficient cell division-specific CRISPR/Cas9 system generates homozygous mutants for multiple genes in Arabidopsis

Feng, Zhengyan, Zhang, Zhengjing, Hua, Kai, Gao, Xifeng, Mao, Yanfei, Botella, Jose Ramon and Zhu, Jian-Kang (2018). A highly efficient cell division-specific CRISPR/Cas9 system generates homozygous mutants for multiple genes in Arabidopsis. International Journal of Molecular Sciences, 19 (12) 3925, 3925. doi: 10.3390/ijms19123925

Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression

Long, Lu, Guo, Dan-Dan, Gao, Wei, Yang, Wen-Wen, Hou, Li-Pan, Ma, Xiao-Nan, Miao, Yu-Chen, Botella, Jose Ramon and Song, Chun-Peng (2018). Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression. Plant Methods, 14 (1) 85, 85. doi: 10.1186/s13007-018-0353-0

Manipulating plant RNA-silencing pathways to improve the gene editing efficiency of CRISPR/Cas9 system

Mao, Yanfei, Yang, Xiaoxuan, Zhou, Yiting, Zhang, Zhengjing, Botella, Jose Ramon and Zhu, Jian-Kang (2018). Manipulating plant RNA-silencing pathways to improve the gene editing efficiency of CRISPR/Cas9 system. Genome Biology, 19 (1) 149, 149. doi: 10.1186/s13059-018-1529-7

A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

Li, Haipeng, Li, Kun, Guo, Yutao, Guo, Jinggong, Miao, Kaiting, Botella, Jose R., Song, Chun-Peng and Miao, Yuchen (2018). A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum). Plant Methods, 14 (1) 50, 50. doi: 10.1186/s13007-018-0319-2

A simple and cost-effective method for screening of CRISPR/Cas9-induced homozygous/biallelic mutants

Guo, Jinggong, Li, Kun, Jin, Lifeng, Xu, Rui, Miao, Kaiting, Yang, Fengbo, Qi, Chaoya, Zhang, Lin, Botella, Jose R., Wang, Ran and Miao, Yuchen (2018). A simple and cost-effective method for screening of CRISPR/Cas9-induced homozygous/biallelic mutants. Plant Methods, 14 (1) 40, 40. doi: 10.1186/s13007-018-0305-8

Correction: Nucleic acid purification from plants, animals and microbes in under 30 seconds (PLoS biology (2017) 15 11 (e2003916))

Zou, Yiping, Mason, Michael Glenn, Wang, Yuling, Wee, Eugene, Turni, Conny, Blackall, Patrick J., Trau, Matt and Botella, Jose Ramon (2018). Correction: Nucleic acid purification from plants, animals and microbes in under 30 seconds (PLoS biology (2017) 15 11 (e2003916)). PLoS Biology, 16 (5). doi: 10.1371/journal.pbio.1002630

Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of the Waxy gene in elite rice varieties

Zhang, Jinshan, Zhang, Hui, Botella, José Ramón and Zhu, Jian-Kang (2018). Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of the Waxy gene in elite rice varieties. Journal of Integrative Plant Biology, 60 (5), 369-375. doi: 10.1111/jipb.12620

Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress

Gao, Wei, Xu, Fu-Chun, Guo, Dan-Dan, Zhao, Jing-Ruo, Liu, Ji, Guo, Ya-Wei, Singh, Prashant Kumar, Ma, Xiao-Nan, Long, Lu, Botella, Jose Ramon and Song, Chun-Peng (2018). Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress. BMC Plant Biology, 18 (1) 15, 1-15. doi: 10.1186/s12870-018-1230-8

Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement

Zhang, Hui, Zhang, Jinshan, Lang, Zhaobo, Botella, José Ramón and Zhu, Jian-Kang (2017). Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement. Critical Reviews in Plant Sciences, 36 (4), 291-309. doi: 10.1080/07352689.2017.1402989

Advanced DNA-Based Point-of-Care Diagnostic Methods for Plant Diseases Detection

Lau, Han Yih and Botella, Jose R. (2017). Advanced DNA-Based Point-of-Care Diagnostic Methods for Plant Diseases Detection. Frontiers in Plant Science, 8 2016, 2016. doi: 10.3389/fpls.2017.02016

Nucleic acid purification from plants, animals and microbes in under 30 seconds

Zou, Yiping, Mason, Michael Glenn, Wang, Yuling, Wee, Eugene, Turni, Conny, Blackall, Patrick J., Trau, Matt and Botella, Jose Ramon (2017). Nucleic acid purification from plants, animals and microbes in under 30 seconds. PLoS Biology, 15 (11) e2003916, e2003916. doi: 10.1371/journal.pbio.2003916

VERNALIZATION1 modulates root system architecture in wheat and barley

Voss-Fels, Kai P., Robinson, Hannah, Mudge, Stephen R., Richard, Cecile, Newman, Saul, Wittkop, Benjamin, Stahl, Andreas, Friedt, Wolfgang, Frisch, Matthias, Gabur, Iulian, Miller-Cooper, Anika, Campbell, Bradley C., Kelly, Alison, Fox, Glen, Christopher, Jack, Christopher, Mandy, Chenu, Karine, Franckowiak, Jerome, Mace, Emma S., Borrell, Andrew K., Eagles, Howard, Jordan, David R., Botella, Jose R., Hammer, Graeme, Godwin, Ian D., Trevaskis, Ben, Snowdon, Rod J. and Hickey, Lee T. (2017). VERNALIZATION1 modulates root system architecture in wheat and barley. Molecular Plant, 11 (1), 226-229. doi: 10.1016/j.molp.2017.10.005

Host Delivered RNAi, an efficient approach to increase rice resistance to sheath blight pathogen (Rhizoctonia solani)

Tiwari, Ila Mukul, Jesuraj, Arun, Kamboj, Richa, Devanna, B. N., Botella, Jose R. and Sharma, T. R. (2017). Host Delivered RNAi, an efficient approach to increase rice resistance to sheath blight pathogen (Rhizoctonia solani). Scientific Reports, 7 (1) 7521, 7521. doi: 10.1038/s41598-017-07749-w

Genome editing in cotton with the CRISPR/Cas9 system

Gao, Wei, Long, Lu, Tian, Xinquan, Xu, Fuchun, Liu, Ji, Singh, Prashant K., Botella, Jose R. and Song, Chunpeng (2017). Genome editing in cotton with the CRISPR/Cas9 system. Frontiers in Plant Science, 8 1364, 1364. doi: 10.3389/fpls.2017.01364

Whole-genome analysis of candidate genes associated with seed size and weight in Sorghum bicolor reveals signatures of artificial selection and insights into parallel domestication in cereal crops

Tao, Yongfu, Mace, Emma S., Tai, Shuaishuai, Cruickshank, Alan, Campbell, Bradley C., Zhao, Xianrong, Van Oosterom, Erik J., Godwin, Ian D., Botella, Jose R. and Jordan, David R. (2017). Whole-genome analysis of candidate genes associated with seed size and weight in Sorghum bicolor reveals signatures of artificial selection and insights into parallel domestication in cereal crops. Frontiers in Plant Science, 8 1237, 1237.1-1237.14. doi: 10.3389/fp/s.2017.01237

Short tandem target mimic rice lines uncover functions of miRNAs in regulating important agronomic traits

Zhang, Hui, Zhang, Jinshan, Yan, Jun, Gou, Feng, Mao, Yanfei, Tang, Guiliang, Botella, Jose Ramon and Zhu, Jian-Kang (2017). Short tandem target mimic rice lines uncover functions of miRNAs in regulating important agronomic traits. Proceedings of the National Academy of Sciences, 114 (20), 5277-5282. doi: 10.1073/pnas.1703752114

Novel chitinase gene LOC_Os11g47510 from indica rice tetep provides enhanced resistance against sheath blight pathogen Rhizoctonia solani in rice

Richa, Kamboj, Tiwari, Ila M., Devanna, B. N., Botella, Jose R., Sharma, Vinay and Sharma, Tilak R. (2017). Novel chitinase gene LOC_Os11g47510 from indica rice tetep provides enhanced resistance against sheath blight pathogen Rhizoctonia solani in rice. Frontiers in Plant Science, 8 596, 596. doi: 10.3389/fpls.2017.00596

Gene targeting by homology-directed repair in rice using a geminivirus-based CRISPR/Cas9 system

Wang, Mugui, Lu, Yuming, Botella, Jose Ramon, Mao, Yanfei, Hua, Kai and Zhu, Jian-kang (2017). Gene targeting by homology-directed repair in rice using a geminivirus-based CRISPR/Cas9 system. Molecular Plant, 10 (7), 1007-1010. doi: 10.1016/j.molp.2017.03.002

Specific and sensitive isothermal electrochemical biosensor for plant pathogen DNA detection with colloidal gold nanoparticles as probes

Lau, Han Yih, Wu, Haoqi, Wee, Eugene J. H., Trau, Matt, Wang, Yuling and Botella, Jose R. (2017). Specific and sensitive isothermal electrochemical biosensor for plant pathogen DNA detection with colloidal gold nanoparticles as probes. Scientific Reports, 7 (1) 38896, 38896. doi: 10.1038/srep38896

Whole genome sequencing reveals potential new targets for improving nitrogen uptake and utilization in Sorghum bicolor

Massel, Karen, Campbell, Bradley C., Mace, Emma S., Tai, Shuaishuai, Tao, Yongfu, Worland, Belinda G., Jordan, David R., Botella, Jose R. and Godwin, Ian D. (2016). Whole genome sequencing reveals potential new targets for improving nitrogen uptake and utilization in Sorghum bicolor. Frontiers in Plant Science, 7 (OCTOBER2016) 1544, 1544. doi: 10.3389/fpls.2016.01544

Heritability of targeted gene modifications induced by plant-optimized CRISPR systems

Mao, Yanfei, Botella, Jose Ramon and Zhu, Jian-Kang (2016). Heritability of targeted gene modifications induced by plant-optimized CRISPR systems. Cellular and Molecular Life Sciences, 74 (6), 1-19. doi: 10.1007/s00018-016-2380-1

Saltational evolution of the heterotrimeric G protein signaling mechanisms in the plant kingdom

Urano, Daisuke, Maruta, Natsumi, Trusov, Yuri, Stoian, Richard, Wu, Qingyu, Liang, Ying, Jaiswal, Dinesh Kumar, Thung, Leena, Jackson, David, Botella, José Ramón and Jones, Alan M. (2016). Saltational evolution of the heterotrimeric G protein signaling mechanisms in the plant kingdom. Science Signaling, 9 (446) ra93, ra93-ra93. doi: 10.1126/scisignal.aaf9558

Field demonstration of a multiplexed point-of-care diagnostic platform for plant pathogens

Lau, Han Yih, Wang, Yuling, Wee, Eugene J. H., Botella, Jose R. and Trau, Matt (2016). Field demonstration of a multiplexed point-of-care diagnostic platform for plant pathogens. Analytical Chemistry, 88 (16), 8074-8081. doi: 10.1021/acs.analchem.6b01551

Heterotrimeric G-proteins facilitate resistance to plant pathogenic viruses in Arabidopsis thaliana (L.) Heynh

Brenya, Eric, Trusov, Yuri, Dietzgen, Ralf Georg and Botella, Jose Ramon (2016). Heterotrimeric G-proteins facilitate resistance to plant pathogenic viruses in Arabidopsis thaliana (L.) Heynh. Plant Signaling and Behavior, 11 (8) e1212798, e1212798. doi: 10.1080/15592324.2016.1212798

Plant G-proteins come of age: breaking the bond with animal models

Trusov, Yuri and Botella, Jose R. (2016). Plant G-proteins come of age: breaking the bond with animal models. Frontiers in Chemistry, 4 (24) 24, 1-9. doi: 10.3389/fchem.2016.00024

Functional characterization of novel chitinase genes present in the sheath blight resistance QTL: Qsbr11-1 in rice line Tetep

Richa, Kamboj, Tiwari, Ila M., Kumari, Mandeep, Devanna, B. N., Sonah, Humira, Kumari, Archana, Nagar, Ramawatar, Sharma, Vinay, Botella, Jose R. and Sharma, Tilak R. (2016). Functional characterization of novel chitinase genes present in the sheath blight resistance QTL: Qsbr11-1 in rice line Tetep. Frontiers in Plant Science, 7 (244) 244, 1-10. doi: 10.3389/fpls.2016.00244

Type B heterotrimeric G protein γ-subunit regulates Auxin and ABA signaling in tomato

Subramaniam, Gayathery, Trusov, Yuri, Lopez-Encina, Carlos, Hayashi, Satomi, Batley, Jacqueline and Botella, Jose Ramon (2016). Type B heterotrimeric G protein γ-subunit regulates Auxin and ABA signaling in tomato. Plant Physiology, 170 (2), 1117-1134. doi: 10.1104/pp.15.01675

Preface

Botella, Jose R. and Botella, Miguel A. (2016). Preface. Methods in Molecular Biology, 1363, vii-x.

A multiplex CRISPR/Cas9 platform for fast and efficient editing of multiple genes in Arabidopsis

Zhang, Zhengjing, Mao, Yanfei, Ha, Si, Liu, Wenshan, Botella, Jose Ramon and Zhu, Jian-Kang (2015). A multiplex CRISPR/Cas9 platform for fast and efficient editing of multiple genes in Arabidopsis. Plant Cell Reports, 35 (7), 1-15. doi: 10.1007/s00299-015-1900-z

Heterotrimeric G proteins interact with defense-related receptor-like kinases in Arabidopsis

Aranda-Sicilia, Maria Nieves, Trusov, Yuri, Maruta, Natsumi, Chakravorty, David, Zhang, Yuelin and Botella, Jose Ramon (2015). Heterotrimeric G proteins interact with defense-related receptor-like kinases in Arabidopsis. Journal of Plant Physiology, 188, 44-48. doi: 10.1016/j.jplph.2015.09.005

A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana

Liu, Wenshan, Zhu, Xiaohong, Lei, Mingguang, Xia, Qingyou, Botella, Jose Ramon, Zhu, Jian-Kang and Mao, Yanfei (2015). A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana. Science Bulletin, 60 (15), 1332-1347. doi: 10.1007/s11434-015-0848-2

TALEN-mediated targeted mutagenesis produces a large variety of heritable mutations in rice

Zhang, Hui, Gou, Feng, Zhang, Jinshan, Liu, Wenshan, Li, Qianqian, Mao, Yanfei, Botella, Jose Ramon and Zhu, Jian-Kang (2015). TALEN-mediated targeted mutagenesis produces a large variety of heritable mutations in rice. Plant Biotechnology Journal, 14 (1), 186-194. doi: 10.1111/pbi.12372

Membrane-localized extra-large G proteins and Gβγ of the heterotrimeric G proteins form functional complexes engaged in plant immunity in arabidopsis

Maruta, Natsumi, Trusov, Yuri, Brenya, Eric, Parekh, Urvi and Botella, Jose Ramon (2015). Membrane-localized extra-large G proteins and Gβγ of the heterotrimeric G proteins form functional complexes engaged in plant immunity in arabidopsis. Plant Physiology, 167 (3), 1004-1016. doi: 10.1104/pp.114.255703

Down-regulation of Fusarium oxysporum endogenous genes by Host-Delivered RNA interference enhances disease resistance

Hu, Zongli, Parekh, Urvi, Maruta, Natsumi, Trusov, Yuri and Botella, Joser R. (2015). Down-regulation of Fusarium oxysporum endogenous genes by Host-Delivered RNA interference enhances disease resistance. Frontiers in Chemistry, 3 (JAN) 1, 1. doi: 10.3389/fchem.2015.00001

Re-purposing bridging flocculation for on-site, rapid, qualitative DNA detection in resource-poor settings

Wee, E. J. H., Lau, H. Y., Botella, J. and Trau, M. (2015). Re-purposing bridging flocculation for on-site, rapid, qualitative DNA detection in resource-poor settings. Chemical Communications, 51 (27), 5828-5831. doi: 10.1039/C4CC10068A

Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis

Mao, Yanfei, Zhang, Zhengjing, Feng, Zhengyan, Wei, Pengliang, Zhang, Hui, Botella, Jose Ramon and Zhu, Jian-Kang (2015). Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis. Plant Biotechnology Journal, 14 (2), 519-532. doi: 10.1111/pbi.12468

Evidence for an unusual transmembrane configuration of AGG3, a class C Gγ subunit of Arabidopsis

Wolfenstetter, Susanne, Chakravorty, David, Kula, Ryan, Urano, Daisuke, Trusov, Yuri, Sheahan, Michael B., McCurdy, David W., Assmann, Sarah M., Jones, Alan M. and Botella, Jose R. (2014). Evidence for an unusual transmembrane configuration of AGG3, a class C Gγ subunit of Arabidopsis. Plant Journal, 81 (3), 388-398. doi: 10.1111/tpj.12732

The pineapple AcMADS1 promoter confers high level expression in tomato and Arabidopsis flowering and fruiting tissues, but AcMADS1 does not complement the tomato LeMADS-RIN (rin) mutant

Moyle, Richard L., Koia, Jonni H., Vrebalov, Julia, Giovannoni, James and Botella, Jose R. (2014). The pineapple AcMADS1 promoter confers high level expression in tomato and Arabidopsis flowering and fruiting tissues, but AcMADS1 does not complement the tomato LeMADS-RIN (rin) mutant. Plant Molecular Biology, 86 (4-5), 395-407. doi: 10.1007/s11103-014-0236-3

Molecular inversion probe: a new tool for highly specific detection of plant pathogens

Lau, Han Yih, Palanisamy, Ramkumar, Trau, Matt and Botella, Jose R. (2014). Molecular inversion probe: a new tool for highly specific detection of plant pathogens. PLoS One, 9 (10) e111182, e111182.1-e111182.9. doi: 10.1371/journal.pone.0111182

EST sequencing of Meloidogyne javanica infected pineapple root tissues reveals changes in gene expression during root-knot nematode induced gall formation

Moyle, Richard L. and Botella, Jose R. (2014). EST sequencing of Meloidogyne javanica infected pineapple root tissues reveals changes in gene expression during root-knot nematode induced gall formation. Tropical Plant Biology, 7 (2), 43-52. doi: 10.1007/s12042-014-9136-6

Heterotrimeric G proteins serve as a converging point in plant defense signaling activated by multiple receptor-like kinases

Liu, Jinman, Ding, Pingtao, Sun, Tongjun, Nitta, Yukino, Dong, Oliver, Huang, Xingchuan, Yang, Wei, Li, Xin, Botella, Jose Ramon and Zhang, Yuelin (2013). Heterotrimeric G proteins serve as a converging point in plant defense signaling activated by multiple receptor-like kinases. Plant Physiology, 161 (4), 2146-2158. doi: 10.1104/pp.112.212431

Heterotrimeric G protein signalling in the plant kingdom

Urano, Daisuke, Chen, Jin-Gui, Botella, Jose Ramon and Jones, Alan M. (2013). Heterotrimeric G protein signalling in the plant kingdom. Open Biology, 3 (MAR) 120186, 1-22. doi: 10.1098/rsob.120186

Pineapple translation factor SUI1 and ribosomal protein L36 promoters drive constitutive transgene expression patterns in Arabidopsis thaliana

Koia, Jonni, Moyle, Richard, Hendry, Caroline, Lim, Lionel and Botella, Jose Ramon (2013). Pineapple translation factor SUI1 and ribosomal protein L36 promoters drive constitutive transgene expression patterns in Arabidopsis thaliana. Plant Molecular Biology, 81 (4-5), 327-336. doi: 10.1007/s11103-012-0002-3

Signaling specificity provided by the Arabidopsis thaliana Heterotrimeric G-Protein gamma subunits AGG1 and AGG2 is partially but not exclusively provided through transcriptional regulation

Thung, Leena, Chakravorty, David, Trusov, Yuri, Jones, Alan M. and Botella, Jose Ramon (2013). Signaling specificity provided by the Arabidopsis thaliana Heterotrimeric G-Protein gamma subunits AGG1 and AGG2 is partially but not exclusively provided through transcriptional regulation. PLoS One, 8 (3) e58503, e58503.1-e58503.11. doi: 10.1371/journal.pone.0058503

Microarray analysis of gene expression profiles in ripening pineapple fruits

Koia, Jonni H., Moyle, Richard L. and Botella, Jose R. (2012). Microarray analysis of gene expression profiles in ripening pineapple fruits. BMC Plant Biology, 12 (1) 240, 240.1-240.13. doi: 10.1186/1471-2229-12-240

Can heterotrimeric G proteins help to feed the world?

Botella, Jose Ramon (2012). Can heterotrimeric G proteins help to feed the world?. Trends in Plant Science, 17 (10), 563-568. doi: 10.1016/j.tplants.2012.06.002

New faces in plant innate immunity: heterotrimeric G proteins

Trusov, Yuri and Botella, Jose Ramon (2012). New faces in plant innate immunity: heterotrimeric G proteins. Journal of Plant Biochemistry and Biotechnology, 21 (1), S40-S47. doi: 10.1007/s13562-012-0140-3

Dissecting Arabidopsis Gβ signal transduction on the protein surface1[W][OA]

Jiang, Kun, Frick-Cheng, Arwen, Trusov, Yuri, Delgado-Cerezo, Magdalena, Rosenthal, David M., Lorek, Justine, Panstruga, Ralph, Booker, Fitzgerald L., Botella, Jose Ramon, Molina, Antonio, Ort, Donald R. and Jones, Alan M. (2012). Dissecting Arabidopsis Gβ signal transduction on the protein surface1[W][OA]. Plant Physiology, 159 (3), 975-983. doi: 10.1104/pp.112.196337

G gamma 1+G gamma 2+G gamma 3=G beta: The search for heterotrimeric G-protein gamma subunits in Arabidopsis is over

Thung, Leena, Trusov, Yuri, Chakravorty, David and Botella, Jose Ramon (2012). G gamma 1+G gamma 2+G gamma 3=G beta: The search for heterotrimeric G-protein gamma subunits in Arabidopsis is over. Journal of Plant Physiology, 169 (5), 542-545. doi: 10.1016/j.jplph.2011.11.010

Site-directed mutagenesis of the Arabidopsis heterotrimeric G protein beta subunit suggests divergent mechanisms of effector activation between plant and animal G proteins

Chakravorty, David, Trusov, Yuri and Botella, Jose Ramon (2012). Site-directed mutagenesis of the Arabidopsis heterotrimeric G protein beta subunit suggests divergent mechanisms of effector activation between plant and animal G proteins. Planta, 235 (3), 615-627. doi: 10.1007/s00425-011-1526-5

Diversity of heterotrimeric G-protein subunits in plants

Trusov, Yuri, Chakravorty, David and Botella, Jose Ramon (2012). Diversity of heterotrimeric G-protein subunits in plants. BMC Research Notes, 5 (1) 608, 608.1-608.9. doi: 10.1186/1756-0500-5-608

Increased plant volatile production affects oviposition, but not larval development, in the moth Helicoverpa armigera

McCallum, Emily J., Cunningham, John Paul, Lücker, Joost, Zalucki, Myron P., De Voss, James J . and Botella, José R. (2011). Increased plant volatile production affects oviposition, but not larval development, in the moth Helicoverpa armigera. Journal of Experimental Biology, 214 (21), 3672-3677. doi: 10.1242/jeb.059923

An atypical heterotrimeric G-protein gamma-subunit is involved in guard cell K+-channel regulation and morphological development in Arabidopsis thaliana

Chakravorty, David, Trusov, Yuri, Zhang, Wei, Acharya, Biswa R., Sheahan, Michael B., McCurdy, David W., Assmann, Sarah M. and Botella, José Ramón (2011). An atypical heterotrimeric G-protein gamma-subunit is involved in guard cell K+-channel regulation and morphological development in Arabidopsis thaliana. Plant Journal, 67 (5), 840-851. doi: 10.1111/j.1365-313X.2011.04638.x

Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis

Klopffleisch, Karsten, Phan, Nguyen, Augustin, Kelsey, Bayne, Robert S., Booker, Katherine S., Botella, Jose R., Carpita, Nicholas C., Carr, Tyrell, Chen, Jin-Gui, Cooke, Thomas Ryan, Frick-Cheng, Arwen, Friedman, Erin J., Fulk, Brandon, Hahn, Michael G., Jiang, Kun, Jorda, Lucia, Kruppe, Lydia, Liu, Chenggang, Lorek, Justine, McCann, Maureen C., Molina, Antonio, Moriyama, Etsuko N., Mukhtar, M. Shahid, Mudgil, Yashwanti, Pattathil, Sivakumar, Schwarz, John, Seta, Steven, Tan, Matthew, Temp, Ulrike ... Jones, Alan M. (2011). Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis. Molecular Systems Biology, 7 (1) 532, 532.1-532.7. doi: 10.1038/msb.2011.66

The 5' untranslated region of the VR-ACS1 mRNA acts as a strong translational enhancer in plants

Wever, Willem, McCallum, Emily J., Chakravorty, David, Cazzonelli, Christopher I. and Botella, José R. (2010). The 5' untranslated region of the VR-ACS1 mRNA acts as a strong translational enhancer in plants. Transgenic Research, 19 (4), 667-674. doi: 10.1007/s11248-009-9332-6

Biochemical characterization of arabidopsis developmentally regulated G-proteins (DRGs)

O'Connell, A, Robin, G, Kobe, B and Botella, JR (2009). Biochemical characterization of arabidopsis developmentally regulated G-proteins (DRGs). PROTEIN EXPRESSION AND PURIFICATION, 67 (2), 88-95. doi: 10.1016/j.pep.2009.05.009

Heterotrimeric G proteins-mediated resistance to necrotrophic pathogens includes mechanisms independent of salicylic acid-, jasmonic acid/ethylene- and abscisic acid-mediated defense signaling

Trusov, Yuri, Sewelam, Nasser, Rookes, James Edward, Kunkel, Matt, Nowak, Ekaterina, Schenk, Peer Martin and Botella, Jose R (2009). Heterotrimeric G proteins-mediated resistance to necrotrophic pathogens includes mechanisms independent of salicylic acid-, jasmonic acid/ethylene- and abscisic acid-mediated defense signaling. The Plant Journal, 58 (1), 69-81. doi: 10.1111/j.1365-313X.2008.03755.x

Recent advances in the development of transgenic papaya technology

Mendoza, E.M.T., Laurena, A.C. and Botella, J.R. (2008). Recent advances in the development of transgenic papaya technology. Biotechnology Annual Review, 14, 423-461. doi: 10.1016/S1387-2656(08)00019-7

G{gamma}1 + G{gamma}2 != Gβ: Heterotrimeric G Protein G{gamma}-Deficient Mutants Do Not Recapitulate All Phenotypes of Gβ-Deficient Mutants

Trusov, Y., Zhang, W., Assmann, S. M. and Botella, J. R. (2008). G{gamma}1 + G{gamma}2 != Gβ: Heterotrimeric G Protein G{gamma}-Deficient Mutants Do Not Recapitulate All Phenotypes of Gβ-Deficient Mutants. Plant Physiology, 147 (2), 636-649. doi: 10.1104/pp.108.117655

Phylogenomic and Functional Analysis of Pterin-4a-Carbinolamine Dehydratase Family (COG2154) Proteins in Plants and Microorganisms

Naponelli, V., Noiriel, A., Ziemak, M.J., Beverley, S.M., Lye, L-F., Plume, A.M., Botella, J.R., Loizeau, K., Ravanel, S., Rébeillé, F., de Crécy-Lagard, V. and Hanson, A.D. (2008). Phylogenomic and Functional Analysis of Pterin-4a-Carbinolamine Dehydratase Family (COG2154) Proteins in Plants and Microorganisms. Plant Physiology, 146 (4), 1515-1527. doi: 10.1104/pp.107.114090

Modulation of higher-plant NAD(H)-dependent glutamate dehydrogenase activity in transgenic tobacco via alteration of beta subunit levels

Purnell, Matthew P., Skopelitis, Damianos S., Roubelakis-Angelakis, Kalliopi A. and Botella, José R. (2007). Modulation of higher-plant NAD(H)-dependent glutamate dehydrogenase activity in transgenic tobacco via alteration of beta subunit levels. Planta, 225 (5), 1325-1325. doi: 10.1007/s00425-007-0481-7

Expression analysis and subcellular localization of the Arabidopsis thaliana G-protein beta-subunit AGB1

Anderson, D. J. and Botella, J. R. (2007). Expression analysis and subcellular localization of the Arabidopsis thaliana G-protein beta-subunit AGB1. Plant Cell Reports, 26 (9), 1469-1480. doi: 10.1007/s00299-007-0356-1

Heterotrimeric G Protein γ Subunits Provide Functional Selectivity in Gßγ Dimer Signaling in Arabidopsis

Trusov, Yuri, Rookes, James Edward, Tilbrook, Kimberley, Chakravorty, David, Mason, Michael Glenn, Anderson, David, Chen, Jin-Gui, Jones, Alan M. and Botella, Jose Ramon (2007). Heterotrimeric G Protein γ Subunits Provide Functional Selectivity in Gßγ Dimer Signaling in Arabidopsis. Plant Cell, 19 (4), 1235-1250. doi: 10.1105/tpc.107.050096

Host-delivered RNAi: an effective strategy to silence genes in plant parasitic nematodes

Fairbairn, David J., Cavallaro, Antonino S., Bernard, Margaret, Mahalinga-Iyer, Janani, Graham, Michael W. and Botella, José R. (2007). Host-delivered RNAi: an effective strategy to silence genes in plant parasitic nematodes. Planta, 226 (6), 1525-1533. doi: 10.1007/s00425-007-0588-x

Over-expression of a truncated Arabidopsis thaliana heterotrimeric G protein gamma subunit results in a phenotype similar to alpha and alpha subunit knockouts

Chakravorty, David and Botella, José Ramón (2007). Over-expression of a truncated Arabidopsis thaliana heterotrimeric G protein gamma subunit results in a phenotype similar to alpha and alpha subunit knockouts. Gene, 393 (1-2), 163-170. doi: 10.1016/j.gene.2007.02.008

Tobacco isoenzyme 1 of NAD(H)-dependent glutamate dehydrogenase catabolizes glutamate in vivo

Purnell, M. P. and Botella, J. R. (2007). Tobacco isoenzyme 1 of NAD(H)-dependent glutamate dehydrogenase catabolizes glutamate in vivo. Plant Physiology, 143 (1), 530-539. doi: 10.1104/pp.106.091330

Heterotrimeric G proteins facilitate Arabidopsis resistance to necrotrophic pathogens and are involved in jasmonate signaling

Trusov, Y., Rookes, J. E., Chakravorty, D., Armour, D., Schenk, P. M. and Botella, J. R. (2006). Heterotrimeric G proteins facilitate Arabidopsis resistance to necrotrophic pathogens and are involved in jasmonate signaling. Plant Physiology, 140 (1), 210-220. doi: 10.1104/pp.105.069625

Silencing of the ACC synthase gene ACACS2 causes delayed flowering in pineapple [Ananas comosus (L.) Merr.]

Trusov, Y. and Botella, J. R. (2006). Silencing of the ACC synthase gene ACACS2 causes delayed flowering in pineapple [Ananas comosus (L.) Merr.]. Journal of Experimental Botany, 57 (14), 3953-3960. doi: 10.1093/jxb/erl167

Characterization of a strong, constitutive mung bean (Vigna radiata L.) promoter with a complex mode of regulation in planta

Cazzonelli, Christopher I., McCallum, Emily J., Lee, Rebecca and Botella, Jose Ramon (2005). Characterization of a strong, constitutive mung bean (Vigna radiata L.) promoter with a complex mode of regulation in planta. Transgenic Research, 14 (6), 941-967. doi: 10.1007/s11248-005-2539-2

Modulation of higher-plant NAD(H)-dependent glutamate dehydrogenase activity in transgenic tobacco via alteration of beta subunit levels

Purnell, M. P., Skopelitis, D. S., Roubelakis-Angelakis, K. A. and Botella, Jose R. (2005). Modulation of higher-plant NAD(H)-dependent glutamate dehydrogenase activity in transgenic tobacco via alteration of beta subunit levels. Planta, 222 (1), 167-180. doi: 10.1007/s00425-005-1510-z

Balancing teaching and research in higher education

Botella, J. R. (2005). Balancing teaching and research in higher education. Studies in Learning Evaluation Innovation and Development, 2 (2), 3-4.

Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division

Petsch, K. A., Botella, J. R. and Mylne, J. (2005). Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division. Plant Physiology, 139 (1), 115-126. doi: 10.1104/pp.105.062695

Developing pineapple fruit has a small transcriptome dominated by metallothionein

Moyle, Richard, Fairbairn, David J., Ripi, Jonni, Crowe, Mark and Botella, Jose R. (2005). Developing pineapple fruit has a small transcriptome dominated by metallothionein. Journal of Experimental Botany, 56 (409), 101-112. doi: 10.1093/jxb/eri015

Hybrid 'Sinta' papaya exhibits unique ACC synthase 1 cDNA isoforms

Hidalgo, M. S. P., Tecson-Mendoza, E. M., Laurena, A. C. and Botella, J. R. (2005). Hybrid 'Sinta' papaya exhibits unique ACC synthase 1 cDNA isoforms. Journal of Biochemistry And Molecular Biology, 38 (3), 320-327. doi: 10.5483/bmbrep.2005.38.3.320

PineappleDB: An online pineapple bioinformatics resource

Moyle, R. L., Ripi-Koia, J., Crowe, M. L., Fairbairn, D. J. and Botella, J. R. (2005). PineappleDB: An online pineapple bioinformatics resource. BMC Plant Biology, 5 (21) 21, 1-5. doi: 10.1186/1471-2229-5-21

Etr1-1 gene expression alters regeneration patterns in transgenic lettuce stimulating root formation

Kim, J. H. and Botella, J. R. (2004). Etr1-1 gene expression alters regeneration patterns in transgenic lettuce stimulating root formation. Plant Cell Tissue And Organ Culture, 78 (1), 69-73. doi: 10.1023/B:TICU.0000020396.64257.c1

Characterisation of three ACC synthase gene family members during post-harvest-induced senescence in broccoli (Brassica oleracea L. var. italica)

Gonzalez, N. and Botella, J. R. (2003). Characterisation of three ACC synthase gene family members during post-harvest-induced senescence in broccoli (Brassica oleracea L. var. italica). Journal of Plant Biology, 46 (4), 223-230. doi: 10.1007/BF03030368

Callus induction and plant regeneration from broccoli (Brassica oleracea var. italica) for transformation

Kim, J. H and Botella, J. R. (2002). Callus induction and plant regeneration from broccoli (Brassica oleracea var. italica) for transformation. Journal of Plant Biology, 45 (3), 177-181. doi: 10.1007/BF03030311

Isolation of a novel G-protein gamma-subunit from Arabidopsis thaliana and its interaction with G beta

Mason, Michael G. and Botella, José R. (2001). Isolation of a novel G-protein gamma-subunit from Arabidopsis thaliana and its interaction with G beta. Biochimica et biophysica acta (BBA). Gene structure and expression, 1520 (2), 147-153. doi: 10.1016/S0167-4781(01)00262-7

An easy and reliable method for establishment and maintenance of leaf and root cell cultures of Arabidopsis thaliana

Encina, CL, Constantin, M and Botella, J (2001). An easy and reliable method for establishment and maintenance of leaf and root cell cultures of Arabidopsis thaliana. Plant Molecular Biology Reporter, 19 (3), 245-248. doi: 10.1007/BF02772896

Re-evaluation of the cytokinin receptor role of the Arabidopsis gene GCR1

Humphrey, Tania Vivienne and Botella, José Ramón (2001). Re-evaluation of the cytokinin receptor role of the Arabidopsis gene GCR1. Journal of Plant Physiology, 158 (5), 645-653. doi: 10.1078/0176-1617-00316

Completing the heterotrimer: Isolation and characterization of an Arabidopsis thaliana G Protein -subunit cDNA

Botella, J. R. and Mason, M. (2000). Completing the heterotrimer: Isolation and characterization of an Arabidopsis thaliana G Protein -subunit cDNA. Proceedings of the National Academy of Sciences of the United States of America, 97 (26), 14784-14788. doi: 10.1073/pnas.97.26.14784

Characterization of ATDRG1, a member of a new class of GTP-binding proteins in plants

Etheridge, N, Trusov, Y, Verbelen, JP and Botella, JR (1999). Characterization of ATDRG1, a member of a new class of GTP-binding proteins in plants. Plant Molecular Biology, 39 (6), 1113-1126. doi: 10.1023/A:1006137221259

Binary vectors for sense and antisense expression of arabidopsis ESTs

Mylne, J and Botella, JR (1998). Binary vectors for sense and antisense expression of arabidopsis ESTs. Plant Molecular Biology Reporter, 16 (3), 257-262. doi: 10.1023/A:1007520410190

Cloning and characterisation of ripening-induced ethylene biosynthetic genes from non-climacteric pineapple (Ananas comosus) fruits

Cazzonelli, CI, Cavallaro, AS and Botella, JR (1998). Cloning and characterisation of ripening-induced ethylene biosynthetic genes from non-climacteric pineapple (Ananas comosus) fruits. Australian Journal of Plant Physiology, 25 (5), 513-518. doi: 10.1071/PP98013

Rapid isolation of high-quality RNA from symbiotic dinoflagellates

Mylne, Joshua S. , Mason, Michael G. and Botella, José R. (1998). Rapid isolation of high-quality RNA from symbiotic dinoflagellates. Phycologia, 37 (4), 307-309. doi: 10.2216/i0031-8884-37-4-307.1

Cloning and characterisation of a glutamate dehydrogenase cDNA from tomato (Lycopersicon esculentum L.)

Purnell, MP, Stewart, GR and Botella, JR (1997). Cloning and characterisation of a glutamate dehydrogenase cDNA from tomato (Lycopersicon esculentum L.). Gene, 186 (2), 249-254. doi: 10.1016/S0378-1119(96)00716-0

Identification and characterisation of two 1-aminocyclopropane-1-carboxylate (ACC) synthase cDNAs expressed during papaya (Carica papaya) fruit ripening

Mason, Michael Glenn and Botella, José Ramón (1997). Identification and characterisation of two 1-aminocyclopropane-1-carboxylate (ACC) synthase cDNAs expressed during papaya (Carica papaya) fruit ripening. Australian Journal of Plant Physiology, 24 (2), 239-244. doi: 10.1071/PP96111

Calcium-dependent protein kinase gene expression in response to physical and chemical stimuli in mungbean (Vigna radiata)

Botella, JR, Arteca, JM, Somodevilla, M and Arteca, RN (1996). Calcium-dependent protein kinase gene expression in response to physical and chemical stimuli in mungbean (Vigna radiata). Plant Molecular Biology, 30 (6), 1129-1137. doi: 10.1007/BF00019547

A mechanical strain-induced 1-aminocyclopropane-1-carboxylic acid synthase gene

Botella, JR, Arteca, RN and Frangos, JA (1995). A mechanical strain-induced 1-aminocyclopropane-1-carboxylic acid synthase gene. Proceedings of the National Academy of Sciences of the United States of America, 92 (5), 1595-1598. doi: 10.1073/pnas.92.5.1595

Tissue-Specific Expression of a Gene Encoding a Cell Wall-Localized Lipid Transfer Protein From Arabidopsis

Thoma, S, Hecht, U, Kippers, A, Botella, J, Devries, S and Somerville, C (1994). Tissue-Specific Expression of a Gene Encoding a Cell Wall-Localized Lipid Transfer Protein From Arabidopsis. Plant Physiology, 105 (1), 35-45. doi: 10.1104/pp.105.1.35

Differential expression of two calmodulin genes in response to physical and chemical stimuli

Botella, JR and Arteca, RN (1994). Differential expression of two calmodulin genes in response to physical and chemical stimuli. Plant Molecular Biology, 24 (5), 757-766. doi: 10.1007/BF00029857

Identification of two new members of the l-aminocyclopropane-l-carboxylate synthase-encoding multigene family in mung bean

Botella, JR, Schlagnhaufer, CD, Arteca, JM, Arteca, RN and Phillips, AT (1993). Identification of two new members of the l-aminocyclopropane-l-carboxylate synthase-encoding multigene family in mung bean. Gene, 123 (2), 249-253. doi: 10.1016/0378-1119(93)90132-M

Identification and characterization of a full-length cDNA encoding for an auxin-induced 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments and expression of its mRNA in response to indole-3-acetic acid

Botella, JR, Arteca, JM, Schlagnhaufer, CD, Arteca, RN and Phillips, AT (1992). Identification and characterization of a full-length cDNA encoding for an auxin-induced 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments and expression of its mRNA in response to indole-3-acetic acid. Plant Molecular Biology, 20 (3), 425-436. doi: 10.1007/BF00040602

Identification and characterization of three putative genes for 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments

Botella, JR, Schlagnhaufer, CD, Arteca, RN and Phillips, AT (1992). Identification and characterization of three putative genes for 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments. Plant Molecular Biology, 18 (4), 793-797. doi: 10.1007/BF00020022

Isolation of a Cdna Clone for Spinach Lipid Transfer Protein and Evidence That the Protein Is Synthesized by the Secretory Pathway

Bernhard, WR, Thoma, S, Botella, J and Somerville, CR (1991). Isolation of a Cdna Clone for Spinach Lipid Transfer Protein and Evidence That the Protein Is Synthesized by the Secretory Pathway. Plant Physiology, 95 (1), 164-170. doi: 10.1104/pp.95.1.164

2 Polypeptides From Green Tomato Leaves Recognized by Antibodies Against Chloroplastic Glutamine-Synthetase

Valpuesta, V, Perezrodriguez, MJ, Quesada, MA and Botella, JR (1989). 2 Polypeptides From Green Tomato Leaves Recognized by Antibodies Against Chloroplastic Glutamine-Synthetase. Plant Physiology and Biochemistry, 27 (6), 963-966.

IMMUNOCYTOLOCALIZATION OF GLUTAMINE-SYNTHETASE IN GREEN LEAVES AND COTYLEDONS OF LYCOPERSICON-ESCULENTUM

BOTELLA, , VERBELEN, JP and VALPUESTA, (1988). IMMUNOCYTOLOCALIZATION OF GLUTAMINE-SYNTHETASE IN GREEN LEAVES AND COTYLEDONS OF LYCOPERSICON-ESCULENTUM. Plant Physiology, 88 (3), 943-946. doi: 10.1104/pp.88.3.943

Immunocytolocalization of ferredoxin-GOGAT in the cells of green leaves and cotyledons of Lycopersicon esculentum

Botella, JR, Verbelen, JP and Valpuesta, V (1988). Immunocytolocalization of ferredoxin-GOGAT in the cells of green leaves and cotyledons of Lycopersicon esculentum. Plant Physiology, 87 (1), 255-257. doi: 10.1104/pp.87.1.255

Different Characteristics of the 2 Glutamate Synthases in the Green Leaves of Lycopersicon-Esculentum

Avila, C, Botella, JR, Canovas, FM, Decastro, IN and Valpuesta, V (1987). Different Characteristics of the 2 Glutamate Synthases in the Green Leaves of Lycopersicon-Esculentum. Plant Physiology, 85 (4), 1036-1039. doi: 10.1104/pp.85.4.1036

Immunocytochemical localization of glutamine synthetase in leaves of tomato seedlings

Botella Mesa, J. R. and Verbelen, J. P. (1987). Immunocytochemical localization of glutamine synthetase in leaves of tomato seedlings. Micron And Microscopica Acta, 18 (3), 219-220. doi: 10.1016/0739-6260(87)90068-2

Immunochemical Comparison of Glutamine Synthetases From Some Solanaceae Plants

Botella, JR, Canovas, FM, Avila, C, Decastro, IN and Valpuesta, V (1986). Immunochemical Comparison of Glutamine Synthetases From Some Solanaceae Plants. Plant Physiology, 82 (2), 585-587. doi: 10.1104/pp.82.2.585

Effect of Light-Dark Transition On Glutamine-Synthetase Activity in Tomato Leaves

Canovas, FM, Avila, C, Botella, JR, Valpuesta, V and Decastro, IN (1986). Effect of Light-Dark Transition On Glutamine-Synthetase Activity in Tomato Leaves. Physiologia Plantarum, 66 (4), 648-652. doi: 10.1111/j.1399-3054.1986.tb05593.x

Delayed flowering in pineapples (Ananas comosus (L.) Merr.) caused by co-suppression of the ACACS2 gene

Trusov, Y. and Botella, J. R. (2006). Delayed flowering in pineapples (Ananas comosus (L.) Merr.) caused by co-suppression of the ACACS2 gene. The 5th International Pineapple Symposium, Port Alfred, South Africa, 11-16 April 2005. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/ActaHortic.2006.702.2

The Pineapple EST sequencing and microarray project

Moyle, R. L., Koia, J., Fairbairn, D. J. and Botella, J. R. (2006). The Pineapple EST sequencing and microarray project. The 5th International Pineapple Symposium, Port Alfred, South Africa, 11-16 April 2005. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/ActaHortic.2006.702.5

Present and future potential of pineapple biotechnology

Botella, J. R. and Fairbairn, D. J. (2005). Present and future potential of pineapple biotechnology. Fourth International Pineapple Symposium, Veracruz, Mexico, 16-19 April 2002. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/ActaHortic.2005.666.1

Towards transformation, regeneration and screening of papaya containing antisense ACC synthase gene

Magdalita, P. M, Laurena, A. C., Yabut-Perez, B. M., Zaporteza, M. M., Tecson-Mendoza, E. M., Villegas, V. N. and Botella, J. R. (2003). Towards transformation, regeneration and screening of papaya containing antisense ACC synthase gene. 10th IAPTC&B Congress, Orlando, Florida, USA, 23-28 June, 2002. DORDRECHT: Springer.

What can molecular biology do for postharvest technology?

Botella, J. R. (2003). What can molecular biology do for postharvest technology?. APEC Symposium on Postharvest Handling Systems, Radison Hotel, Bangkok, Thailand, 1-3 September, 2003.

Bigger, firmer, riper. Biotechnological control of ripening in tropical fruits

Botella, J. R. (2002). Bigger, firmer, riper. Biotechnological control of ripening in tropical fruits. The International Symposium on Tropical and Subtropical Fruits, Cairns, Australia, 26 Nov - 1 Dec 2000. Leuven, Belgium: ISHS. doi: 10.17660/ActaHortic.2002.575.69

Clonging and molecular characterization of ripening-related ACC synthase from papaya fruit (Carica papaya L.)

Laurena, A. C., Magdalita, P. M., Hidalgo, M. S. P., Villegas, V. N., Mendoza, E. M. T. and Botella, J. R. (2002). Clonging and molecular characterization of ripening-related ACC synthase from papaya fruit (Carica papaya L.). The International Symposium on Tropical and Subtropical Fruits, Cairns, Australia, 26 Nov - 1 Dec 2000. Leuven, Belgium: ISHS. doi: 10.17660/ActaHortic.2002.575.16

Progress in the development of transgenic papaya: transformation of solo papaya using ACC synthase antisense construct

Magdalita, P. M., Laurena, A. C., Yabut-Perez, B.M., Tecson-Mendoza, E. M., Villegas, V. N. and Botella, J. R. (2002). Progress in the development of transgenic papaya: transformation of solo papaya using ACC synthase antisense construct. The International Symposium on Tropical and Subtropical Fruits, Cairns, Australia, 26 Nov - 1 Dec 2000. Leuven, Belgium: ISHS. doi: 10.17660/ActaHortic.2002.575.17

Biolistic Transformation of papaya cv sunrise solo

Cavallaro, A. S., Leeton, P. R. J. and Botella, J. R. (2000). Biolistic Transformation of papaya cv sunrise solo. Combio 2000, Wellington Festival and Convention Center, NZ, 11-14 December 2000. Kent Town, SA: Australian Society for Biochemistry and Molecular Biology.

Characterisation of a novel G protein interactor in Arabidopsis

Plume, A. M. and Botella, J. R. (2000). Characterisation of a novel G protein interactor in Arabidopsis. Com Bio 2000, Wellington, NZ, 11-14 Dec, 2000. Vic, Australia: Australian Society for Biochemistry and Molecular Biology, Inc.

Differential expression of the ACC Synthase and ACC Oxidase enzymes involved in fruit ripening in papaya

Botella, J. R., Leeton, P. R. J. and Cavallaro, A. S. (2000). Differential expression of the ACC Synthase and ACC Oxidase enzymes involved in fruit ripening in papaya. Combio 2000, Wellington Festival and Convention Center, NZ, 11-14 December 2000. Kent Town, SA: Australian Society for Biochemistry and Molecular Biology.

Freaky Floral Bouquets! Over-expression of the 'Broomhead' gene in Arabidopsis

Petsch, K. A., Mylne, J. S. and Botella, J. R. (2000). Freaky Floral Bouquets! Over-expression of the 'Broomhead' gene in Arabidopsis. Com Bio 2000, Wellington, NZ, 11-14 Dec, 2000. Kent Town, S.A., Australia: Australian Society for Biochemistry and Molecular Biology.

Is the hunt over? A role for the elusive enzyme GDH

Purnell, M. P. and Botella, J. R. (2000). Is the hunt over? A role for the elusive enzyme GDH. Com Bio 2000, Wellington, NZ, 11-14 Dec, 2000. Victoria, Australia: Australian Society for Biochemistry and Molecular Biology, Inc.

Oh My God, we are eating genes! the myth and reality of GM Foods

Botella, J. R. (2000). Oh My God, we are eating genes! the myth and reality of GM Foods. ABA 2000 Biotechnology: Our Passport to the Next Millenium, Brisbane, 2-6 July, 2000. Brisbane Australia: Australian Biotechnology Association.

On the trail of G Proteins in plants

Anderson, D. and Botella, J. R. (2000). On the trail of G Proteins in plants. Com Bio 2000, Wellington, NZ, 11-14 Dec, 2000. Vic, Australia: Australian Society for Biochemistry and Molecular Biology, Inc.

Plant heterotrimeric G-Protein pathways exposed

Mason, M. and Botella, J. R. (2000). Plant heterotrimeric G-Protein pathways exposed. Com Bio 2000, Wellington, NZ, 11-14 Dec, 2000. Vic, Australia: Australian Society for Biochemistry and Molecular Biology, Inc.

Plentiful's Tale

Ferris, R., Mylne, J. S. and Botella, J. R. (2000). Plentiful's Tale. Com Bio 2000, Wellington, NZ, 11-14 Dec, 2000. Vic, Australia: Australian Society for Biochemistry and Molecular Biology, Inc.

Re-evaluating the receptor of the Arabidopsis gene GCR1

Humphrey, T. and Botella, J. R. (2000). Re-evaluating the receptor of the Arabidopsis gene GCR1. Com Bio 2000, Wellington Festival and Convention Center, NZ, 11-14 Dec, 2000. Victoria, Australia: Australian Society for Biochemistry and Molecular Biology, Inc.

The Arabidopsis member of the developmentally regulated GTP-binding proteins:AtDRG1

Etheridge, N. and Botella, J. R. (2000). The Arabidopsis member of the developmentally regulated GTP-binding proteins:AtDRG1. Com Bio 2000, Wellington, NZ, 11-14 Dec, 2000. Vic, Australia: Australian Society for Biochemistry and Molecular Biology, Inc.

Towards the production of transgenic pineapple to control flowering and ripening

Cazzonelli, C., Cavallaro, A. S. and Botella, J. R. (2000). Towards the production of transgenic pineapple to control flowering and ripening. 3rd International Pineapple Symposium, Pattaya, Thailand, 17-20 November 1998. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/ActaHortic.2000.529.13

Transient and stable transformation in mango by particle bombardment

Cruz-Hernandez, A., Town, L. J., Cavallaro, A. S. and Botella, J. R. (2000). Transient and stable transformation in mango by particle bombardment. Sixth International Symposium on Mango, Pattaya City, Thailand, 6-9 April 1999. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/ActaHortic.2000.509.24

Control of ripening in papaya and mango by genetic engineering

Botella, J. R., Cruz-Hernandez, A., Mason, M., Leeton, P. R. J., Cavallaro, A. S. and Labrie, P. (1999). Control of ripening in papaya and mango by genetic engineering. Int'l Symp on Plant Genetic Eng: Towards the Third Millen, Cuba, 6-10 Dec, 99. La Habana, Cuba: Elfos Scientiae.

Searching for the role of ethlene in non-climacteric fruits

Cazzonelli, C., Cavallaro, A. S. and Botella, J. R. (1999). Searching for the role of ethlene in non-climacteric fruits. Proceedings of the EU-TMR-Euro Conference Symp on Biol, Thira (Santorini), Greece, 5-8 Sept, 99. Dordrecht, The Netherlands: Kluwer Academic Publishers.

Isolation and genetic manipulation of invertase genes in sugarcane

Peters, KF, Grof, CPL, Botella, J and Albert, H (1996). Isolation and genetic manipulation of invertase genes in sugarcane. International Symposium on Sugar 2000, Brisbane Australia, Aug 19-23, 1996. EAST MELBOURNE: C S I R O.

Mungbean Calcium-Dependent Protein-Kinase Cdna and its Expression in Response to Physical and Chemical Stimuli

Botella, JR, Arteca, JM, Somodevilla, M and Arteca, RN (1995). Mungbean Calcium-Dependent Protein-Kinase Cdna and its Expression in Response to Physical and Chemical Stimuli. ROCKVILLE: AMER SOC PLANT PHYSIOLOGISTS.

A Multigene Family for Protein-Kinases in Mung Bean

Botella, JR and Arteca, RN (1993). A Multigene Family for Protein-Kinases in Mung Bean. ROCKVILLE: AMER SOC PLANT PHYSIOLOGISTS.

Differential Expression of 2 Calmodulin Genes in Mung Bean

Botella, JR and Arteca, RN (1993). Differential Expression of 2 Calmodulin Genes in Mung Bean. ROCKVILLE: AMER SOC PLANT PHYSIOLOGISTS.

Effects of Plant Hormones On Acc Synthase Gene-Expression in Etiolated Mung Beans

Arteca, JM, Botella, JR and Arteca, RN (1993). Effects of Plant Hormones On Acc Synthase Gene-Expression in Etiolated Mung Beans. ROCKVILLE: AMER SOC PLANT PHYSIOLOGISTS.

Relationship Between Calcium and Ethylene Biosynthesis in Etiolated Mung Bean

Arteca, JM, Arteca, RN and Botella, JR (1993). Relationship Between Calcium and Ethylene Biosynthesis in Etiolated Mung Bean. ROCKVILLE: AMER SOC PLANT PHYSIOLOGISTS.

A method for facilitating pathogen resistance

Botella, J. R., Graham, M. and Fairbairn, D. J. (2002). A method for facilitating pathogen resistance. 2002301927.