Professor Bostjan Kobe

ARC Laureate Fellow

School of Chemistry and Molecular Biosciences
Faculty of Science

Affiliate Prof Research Fellow

Institute for Molecular Bioscience
b.kobe@uq.edu.au
+61 7 336 52132

Overview

Dr Kobe's research interests are in protein structure and function, focussing particularly on proteins involved in the processes of infection and immunity.

The primary techniques used in the laboratory are X-ray crystallography and cryo-electron microscopy, combined with a plethora of other molecular biology, biophysical and computational techniques.

Current projects include:

  • Structural basis of signalling by cooperative assembly formation (SCAF) in innate immunity and cell-death pathways, in particular Toll-like receptors and SARM1
  • Structural basis of plant disease resistance - particularly plant NLRs and the correponding pathogen effectro proteins
  • Molecular and structural basis of function of proteins from pathogens (fungal, bacterial and viral pathogens)

Research Impacts

We focus on basic research that provides a foundsation for applications in medicine (inflammatory, neurodegenerative and infectious disease) anf agriculture (improving plant disease resistance).

Qualifications

  • Doctor of Philosophy, University of Texas
  • Diploma Engineer of Chemistry, UL

Publications

  • Clabbers, Max T. B., Holmes, Susannah, Muusse, Timothy W., Vajjhala, Parimala R., Thygesen, Sara J., Malde, Alpeshkumar K., Hunter, Dominic J. B., Croll, Tristan I., Flueckiger, Leonie, Nanson, Jeffrey D., Rahaman, Md. Habibur, Aquila, Andrew, Hunter, Mark S., Liang, Mengning, Yoon, Chun Hong, Zhao, Jingjing, Zatsepin, Nadia A., Abbey, Brian, Sierecki, Emma, Gambin, Yann, Stacey, Katryn J., Darmanin, Connie, Kobe, Bostjan, Xu, Hongyi and Ve, Thomas (2021). MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography. Nature Communications, 12 (1) 2578, 1-14. doi: 10.1038/s41467-021-22590-6

  • Figley, Matthew D., Gu, Weixi, Nanson, Jeffrey D., Shi, Yun, Sasaki, Yo, Cunnea, Katie, Malde, Alpeshkumar K., Jia, Xinying, Luo, Zhenyao, Saikot, Forhad K., Mosaiab, Tamim, Masic, Veronika, Holt, Stephanie, Hartley-Tassell, Lauren, McGuinness, Helen Y., Manik, Mohammad K., Bosanac, Todd, Landsberg, Michael J., Kerry, Philip S., Mobli, Mehdi, Hughes, Robert O., Milbrandt, Jeffrey, Kobe, Bostjan, DiAntonio, Aaron and Ve, Thomas (2021). SARM1 is a metabolic sensor activated by an increased NMN/NAD+ ratio to trigger axon degeneration. Neuron, 109 (7), 1-19. doi: 10.1016/j.neuron.2021.02.009

  • Luo, Zhenyao, Morey, Jacqueline R., Deplazes, Evelyne, Motygullina, Alina, Tan, Aimee, Ganio, Katherine, Neville, Stephanie L., Eleftheriadis, Nikolaos, Isselstein, Michael, Pederick, Victoria G., Paton, James C., Cordes, Thorben, Harmer, Jeffrey R., Kobe, Bostjan and McDevitt, Christopher A. (2021). A trap-door mechanism for zinc acquisition by Streptococcus pneumoniae AdcA. mBio, 12 (1) e01958-20, 1-14. doi: 10.1128/mBio.01958-20

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Supervision

View all Supervision

Available Projects

View all Available Projects

Publications

Book Chapter

  • Ellis, Jonathan J. and Kobe, Boštjan (2013). Protein phosphorylation. Encyclopedia of biophysics. (pp. 2037-2040) edited by Gordon C. K. Roberts. Berlin, Germany: Springer-Verlag. doi: 10.1007/978-3-642-16712-6_184

  • Kobe, Bostjan and Kemp, Bruce E. (2010). Principles of kinase regulation. Handbook of Cell Signaling, 2/e. (pp. 559-563) Elsevier Inc.. doi: 10.1016/B978-0-12-374145-5.00074-7

  • Mouradov, Dmitri, Kobe, Bostjan, Dixon, Nicholas E. and Huber, Thomas (2010). Hybrid methods for protein structure prediction. Introduction to protein structure prediction: Methods and algorithms. (pp. 265-277) edited by Huzefa Rangwala and George Karypis. Hoboken, New Jersey, USA: John Wiley and Sons, Inc.. doi: 10.1002/9780470882207.ch12

  • W. Saunders, Neil F., Brinkworth, Ross I., Kemp, Bruce E. and Kobe, Bostjan (2010). Substrates of Cyclic Nucleotide-Dependent Protein Kinases. Handbook of Cell Signaling. (pp. 1489-1495) Elsevier. doi: 10.1016/b978-0-12-374145-5.00182-0

  • Li, Sheng, Mouradov, Dmitri, King, Gordon, Liu, Tong, Ross, Ian, Kobe, Bostjan, Woods, Virgil L. and Huber, Thomas (2009). Study of protein three-dimensional structure and dynamics determination using peptide amide hydrogen/deuterium exchange mass spectrometry (DXMS) and chemical cross-linking with mass spectrometry to constrain molecular modeling. Structural Bioinformatics. (pp. 171-206) edited by Gu, Jenny and Bourne, Phillip E.. Hoboken, N.J.: Wiley-Blackwell.

  • Robin, G., Cowieson, N.P., Guncar, G., Forwood, J.K., Listwan, P., Hume, D.A., Kobe, B., Martin, J.L. and Huber, T.L. (2008). A general target selection method for crystallographic proteomics. Structural Proteomics: High-Throughput Methods. (pp. 27-35) edited by Kobe, B., Guss, M. and Huber, T.L.. Totowa, NJ, United States: Humana Press. doi: 10.1007/978-1-60327-058-8_2

  • Cowieson, N. P., Wensley, B., Robin, G., Guncar, G., Forwood, J. K., Hume, D. A., Kobe, B. and Martin, J. L. (2008). A medium or high throughput protein refolding assay. Structural Proteomics: High throughput Methods. (pp. 269-275) edited by Bostjan Kobe, Mitchell Guss and Thomas Huber. Totowa, NJ, United States: Humana Press. doi: 10.1007/978-1-60327-058-8_17

  • Thakur, A. S., Newman, J., Martin, J. L. and Kobe, B. (2008). Increasing protein crystallization screening success with heterogeneous nucleating agents. Structural Proteomics: High-throughput Methods. (pp. 403-409) edited by B. Kobe, M. Guss and T. Huber. USA: Humana Press.

  • Meng, Weining, Forwood, Jade K., Guncar, Gregor, Robin, Gautier, Cowieson, Nathan P., Listwan, Pawel, Mouradov, Dmitri, King, Gordon, Ross, lan L., Robinson, Jodie, Puri, Munish, Hill, Justine M., Kellie, Stuart, Huber, Thomas, Hume, David A., Martin, Jennifer L. and Kobe, Bostjan (2008). Overview of the pipeline for structural and functional characterization of macrophage proteins at the University of Queensland. Structural proteomics: High-throughput methods. (pp. 577-587) edited by B. Kobe, M. Guss and T. L. Huber. Totowa, NJ, United States: Humana Press.

  • Mouradov, Dmitri, King. Gordon, Ross, Ian L., Forwood, Jade K., Hume, David A., Sinz, Andrea, Martin, Jennifer L., Kobe, Bostjan and Huber, Thomas L. (2008). Protein structure determination using a combination of cross-linking, mass spectrometry, and molecular modeling. Structural Proteomics: High Throughput Methods. (pp. 459-474) edited by Bostjan Kobe, Mitchell Guss and Thomas L. Huber. Totowa, NJ, United States: Humana Press. doi: 10.1007/978-1-60327-058-8_31

  • Brinkworth, R. I., Kobe, B. and Kemp, B.L.E. (2004). Peptide substrates of cyclic nucleotide-dependent protein kinases. Handbook of Cell Signaling. (pp. 495-499) edited by R. A. Bradshaw and E. A. Dennis. USA: Academic Press. doi: 10.1016/B978-012124546-7/50563-5

  • Kobe, B. and Kemp, B. E. (2004). Principles of kinase regulation. Handbook of Cell Signaling Vol 1. (pp. 539-542) edited by R. A. Bradshaw and E. A. Dennis. USA: Academic Press.

  • Kobe, Bostjan and Kemp, Bruce E. (2003). Principles of Kinase Regulation. Handbook of Cell Signaling. (pp. 539-542) Elsevier Inc.. doi: 10.1016/B978-012124546-7/50450-2

  • Brinkworth, R. I., Breinl, R. A. and Kobe, B. (2002). Conserved sequence motifs in protein kinase families. Recent Research Developments in Protein Folding Stability and Design. (pp. 67-77) edited by M Michael Gromiha and S Selvaraj. Trivandrum, India: Research Signpost.

  • Kobe, Bostjan, Jennings, Ian G. and Cotton, Richard G. H. (2002). Structure and Regulation of Phenylalanine Hydroxylase, and Implications for Related Enzymes. Chemistry and Biology of Pteridines and Folates. (pp. 79-84) Boston, MA: Springer US. doi: 10.1007/978-1-4615-0945-5_13

  • Heierhorst, Jorg, Pearson, Richard, Horne, James, Bozinovski, Steven, Kobe, Bostjan and Kemp, Bruce E. (2000). Protein serine/threonine kinases. Principles of Molecular Regulation. (pp. 297-310) edited by Michael Conn P. and Anthony R. Means. Totowa, N.J., USA: Humana Press. doi: 10.1007/978-1-59259-032-2_17

  • Kobe, Bostjan, Gleichmann, Thomas, Teh, Trazel, Heierhorst, Jorg and Kemp, Bruce E. (1999). Crystallization of protein kinases and phosphatases. Protein phosphorylation: A practical approach. (pp. 387-422) edited by D. Grahame Hardie. Oxford, UK: Oxford University Press.

  • Kobe, Boštjan, Heierhorst, Jörg and Kemp, Bruce E. (1997). 3 Intrasteric regulation of protein kinases. Advances in Second Messenger and Phosphoprotein Research. (pp. 29-40) Elsevier. doi: 10.1016/s1040-7952(97)80006-7

  • Rizo, Josep, Blanco, Francisco, Kobe, Bostjan, Bruch, Martha D., Hoyt, David W. and Gierasch, Lila M. (1992). Conformations of wild-type and mutant OmpA signal sequences in membrane mimetic environments. Peptides. (pp. 265-267) Dordrecht: Springer Netherlands. doi: 10.1007/978-94-011-2264-1_94

Journal Article

Conference Publication

Grants (Administered at UQ)

PhD and MPhil Supervision

Current Supervision

  • Doctor Philosophy — Principal Advisor

  • Doctor Philosophy — Principal Advisor

    Other advisors:

  • Doctor Philosophy — Principal Advisor

  • Doctor Philosophy — Principal Advisor

  • Doctor Philosophy — Principal Advisor

  • Doctor Philosophy — Principal Advisor

  • Doctor Philosophy — Principal Advisor

  • Doctor Philosophy — Principal Advisor

  • Doctor Philosophy — Principal Advisor

  • Doctor Philosophy — Associate Advisor

  • Doctor Philosophy — Associate Advisor

    Other advisors:

  • Doctor Philosophy — Associate Advisor

    Other advisors:

  • Doctor Philosophy — Associate Advisor

  • Doctor Philosophy — Associate Advisor

  • Doctor Philosophy — Associate Advisor

  • Doctor Philosophy — Associate Advisor

Completed Supervision

Possible Research Projects

Note for students: The possible research projects listed on this page may not be comprehensive or up to date. Always feel free to contact the staff for more information, and also with your own research ideas.