The University of Queensland

Molecular engineering of Conus peptides as therapeutic leads

Daniel, James T. and Clark, Richard J. (2017). Molecular engineering of Conus peptides as therapeutic leads. Peptides and peptide-based biomaterials and their biomedical applications. (pp. 229-254) edited by Anwar Sunna, Andrew Care and Peter L. Bergquist. Cham, Switzerland: Springer. doi: 10.1007/978-3-319-66095-0_10

NMR-based screening and drug discovery

Craik, David J., Smith, Philippa A. and Clark, Richard James (2010). NMR-based screening and drug discovery. Burger's medicinal chemistry, drug discovery, and development. (pp. 359-437) edited by Donald J. Abraham, David P. Rotella and Alfred Burger. Hoboken, N.J.: Wiley. doi: 10.1002/0471266949

Structure-based Drug Design and NMR-based screening

Craik, D. J. and Clark, R. J. (2008). Structure-based Drug Design and NMR-based screening. Pharmacology from drug development to gene therapy. (pp. 225-313) edited by R. A. Meyers. Weinheim: Wiley-VCH Verlag GMBH & Co.

Structure-based drug design and NMR-based screening

Craik, D. J. and Clark, R. J. (2005). Structure-based drug design and NMR-based screening. Encyclopdeia of Molecular Cell Biology and Molecular Medicine. (pp. 517-605) edited by Robert A Meyers. Germany: Wiley-VCH.

NMR and Drug Discovery

Craik, D. J. and Clark, R. J. (2003). NMR and Drug Discovery. Burger's Medicinal Chemistry and Drug Discovery, Vol 1, Drug Discovery. (pp. 507-582) edited by Donald J Abraham. USA: John Wiley & Sons. doi: 10.1002/0471266949.bmc011

Special Issue in Honor of Professor Mary Garson AM

Blanchfield, Joanne T., Nonato, Maribel G. and Clark, Richard J. (2023). Special Issue in Honor of Professor Mary Garson AM. Journal of Natural Products, 86 (3), 473-474. doi: 10.1021/acs.jnatprod.3c00088

TLQP-21 is a low potency partial C3aR activator on human primary macrophages

Li, Xaria X., Lee, John D., Lee, Han S., Clark, Richard J. and Woodruff, Trent M. (2023). TLQP-21 is a low potency partial C3aR activator on human primary macrophages. Frontiers in Immunology, 14 1086673, 1-10. doi: 10.3389/fimmu.2023.1086673

Author Correction: Tick holocyclotoxins trigger host paralysis by presynaptic inhibition

Chand, Kirat K., Lee, Kah Meng, Lavidis, Nickolas A., Rodriguez-Valle, Manuel, Ijaz, Hina, Koehbach, Johannes, Clark, Richard J., Lew-Tabor, Ala and Noakes, Peter G. (2022). Author Correction: Tick holocyclotoxins trigger host paralysis by presynaptic inhibition. Scientific Reports, 12 (1) 20636, 1-1. doi: 10.1038/s41598-022-24528-4

Engineering peptide inhibitors of the HFE–transferrin receptor 1 complex

Goncalves Monteiro, Daniela Goncalves, Rishi, Gautam, Gorman, Declan M., Burnet, Guillaume, Aliyanto, Randy, Rosengren, K. Johan, Frazer, David M., Subramaniam, V. Nathan and Clark, Richard J. (2022). Engineering peptide inhibitors of the HFE–transferrin receptor 1 complex. Molecules, 27 (19) 6581, 1-13. doi: 10.3390/molecules27196581

SARS-CoV-2 triggers complement activation through interactions with heparan sulfate

Lo, Martin W., Amarilla, Alberto A., Lee, John D., Albornoz, Eduardo A., Modhiran, Naphak, Clark, Richard J., Ferro, Vito, Chhabra, Mohit, Khromykh, Alexander A., Watterson, Daniel and Woodruff, Trent M. (2022). SARS-CoV-2 triggers complement activation through interactions with heparan sulfate. Clinical and Translational Immunology, 11 (8) e1413, e1413. doi: 10.1002/cti2.1413

The structural conformation of the tachykinin domain drives the anti‐tumoral activity of an octopus peptide in melanoma BRAF ^V600E

Moral‐Sanz, Javier, Fernandez‐Rojo, Manuel A., Colmenarejo, Gonzalo, Kurdyukov, Sergey, Brust, Andreas, Ragnarsson, Lotten, Andersson, Åsa, Vila, Sabela F., Cabezas‐Sainz, Pablo, Wilhelm, Patrick, Vela‐Sebastian, Ana, Fernández‐Carrasco, Isabel, Chin, Yanni K. Y., López‐ Mancheño, Yaiza, Smallwood, Taylor B., Clark, Richard J., Fry, Bryan G., King, Glenn F., Ramm, Grant A., Alewood, Paul F., Lewis, Richard J., Mulvenna, Jason P., Boyle, Glen M., Sanchez, Laura E., Neely, G. Gregory, Miles, John J. and Ikonomopoulou, Maria P. (2022). The structural conformation of the tachykinin domain drives the anti‐tumoral activity of an octopus peptide in melanoma BRAF V600E. British Journal of Pharmacology, 179 (20), 4878-4896. doi: 10.1111/bph.15923

Mitochondrial C5aR1 activity in macrophages controls IL-1β production underlying sterile inflammation

Niyonzima, Nathalie, Rahman, Jubayer, Kunz, Natalia, West, Erin E., Freiwald, Tilo, Desai, Jigar V., Merle, Nicolas S., Gidon, Alexandre, Sporsheim, Bjørnar, Lionakis, Michail S., Evensen, Kristin, Lindberg, Beate, Skagen, Karolina, Skjelland, Mona, Singh, Parul, Haug, Markus, Ruseva, Marieta M., Kolev, Martin, Bibby, Jack, Marshall, Olivia, O’Brien, Brett, Deeks, Nigel, Afzali, Behdad, Clark, Richard J., Woodruff, Trent M., Pryor, Milton, Yang, Zhi-Hong, Remaley, Alan T., Mollnes, Tom E. ... Kemper, Claudia (2021). Mitochondrial C5aR1 activity in macrophages controls IL-1β production underlying sterile inflammation. Science Immunology, 6 (66) eabf2489, eabf2489. doi: 10.1126/sciimmunol.abf2489

In vivo pharmacodynamic method to assess complement C5a receptor antagonist efficacy

Cui, Cedric S., Kumar, Vinod, Gorman, Declan M., Clark, Richard J., Lee, John D. and Woodruff, Trent M. (2021). In vivo pharmacodynamic method to assess complement C5a receptor antagonist efficacy. ACS Pharmacology and Translational Science, 5 (1) acsptsci.1c00227, 41-51. doi: 10.1021/acsptsci.1c00227

Solution NMR and racemic crystallography provide insights into a novel structural class of cyclic plant peptides

Payne, Colton D., Vadlamani, Grishma, Hajiaghaalipour, Fatemeh, Muhammad, Taj, Fisher, Mark F., Andersson, Håkan S., Göransson, Ulf, Clark, Richard J., Bond, Charles S., Mylne, Joshua S. and Rosengren, K. Johan (2021). Solution NMR and racemic crystallography provide insights into a novel structural class of cyclic plant peptides. RSC Chemical Biology, 2 (6), 1682-1691. doi: 10.1039/d1cb00155h

Unexpected off-target activities for recombinant C5a in human macrophages

Li, Xaria X., Gorman, Declan M., Lee, John D., Clark, Richard J. and Woodruff, Trent M. (2021). Unexpected off-target activities for recombinant C5a in human macrophages. Journal of Immunology, 208 (1), 133-142. doi: 10.4049/jimmunol.2100444

Biostimulation of bacteria in liquid culture for identification of new antimicrobial compounds

Mirzaee, Hooman, Ariens, Emily, Blaskovich, Mark A. T., Clark, Richard J. and Schenk, Peer M. (2021). Biostimulation of bacteria in liquid culture for identification of new antimicrobial compounds. Pharmaceuticals, 14 (12) 1232, 1232. doi: 10.3390/ph14121232

A conserved β‐bulge glycine residue facilitates folding and increases stability of the mouse α‐defensin cryptdin‐4

Clark, Richard J., Phan, Thanh Huyen, Song, Angela, Ouellette, André J., Conibear, Anne C. and Rosengren, K. Johan (2021). A conserved β‐bulge glycine residue facilitates folding and increases stability of the mouse α‐defensin cryptdin‐4. Peptide Science, 114 (1) e24250. doi: 10.1002/pep2.24250

Development of synthetic human and mouse C5a: application to binding and functional assays in vitro and in vivo

Gorman, Declan M., Li, Xaria X., Payne, Colton D., Cui, Cedric S., Lee, John D., Rosengren, K. Johan, Woodruff, Trent M. and Clark, Richard J. (2021). Development of synthetic human and mouse C5a: application to binding and functional assays in vitro and in vivo. ACS Pharmacology and Translational Science, 4 (6) acsptsci.1c00199, 1808-1817. doi: 10.1021/acsptsci.1c00199

Development of Potent and Selective Agonists for Complement C5a Receptor 1 with In Vivo Activity

Gorman, Declan M., Li, Xaria X., Lee, John D., Fung, Jenny N., Cui, Cedric S., Lee, Han Siean, Rolfe, Barbara E., Woodruff, Trent M. and Clark, Richard J. (2021). Development of Potent and Selective Agonists for Complement C5a Receptor 1 with In Vivo Activity. Journal of Medicinal Chemistry, 64 (22) acs.jmedchem.1c01174, 16598-16608. doi: 10.1021/acs.jmedchem.1c01174

Anaphylatoxin receptor promiscuity for commonly used complement C5a peptide agonists

Li, Xaria X., Clark, Richard J. and Woodruff, Trent M. (2021). Anaphylatoxin receptor promiscuity for commonly used complement C5a peptide agonists. International Immunopharmacology, 100 108074, 108074. doi: 10.1016/j.intimp.2021.108074

LEAP-2: an emerging endogenous ghrelin receptor antagonist in the pathophysiology of obesity

Lu, Xuehan, Huang, Lili, Huang, Zhengxiang, Feng, Dandan, Clark, Richard J. and Chen, Chen (2021). LEAP-2: an emerging endogenous ghrelin receptor antagonist in the pathophysiology of obesity. Frontiers in Endocrinology, 12 717544, 717544. doi: 10.3389/fendo.2021.717544

Synthetic hookworm-derived peptides are potent modulators of primary human immune cell function that protect against experimental colitis in vivo

Smallwood, Taylor B., Navarro, Severine, Cristofori-Armstrong, Ben, Watkins, Thomas S., Tungatt, Katie, Ryan, Rachael Y.M., Haigh, Oscar L., Lutzky, Viviana P., Mulvenna, Jason P., Rosengren, K. Johan, Loukas, Alex, Miles, John J. and Clark, Richard J. (2021). Synthetic hookworm-derived peptides are potent modulators of primary human immune cell function that protect against experimental colitis in vivo. Journal of Biological Chemistry, 297 (1) 100834, 1-14. doi: 10.1016/j.jbc.2021.100834

Design of a stable cyclic peptide analgesic derived from sunflower seeds that targets the κ-opioid receptor for the treatment of chronic abdominal pain

Muratspahić, Edin, Tomašević, Nataša, Koehbach, Johannes, Duerrauer, Leopold, Hadžić, Seid, Castro, Joel, Schober, Gudrun, Sideromenos, Spyridon, Clark, Richard J., Brierley, Stuart M., Craik, David J. and Gruber, Christian W. (2021). Design of a stable cyclic peptide analgesic derived from sunflower seeds that targets the κ-opioid receptor for the treatment of chronic abdominal pain. Journal of Medicinal Chemistry, 64 (13) acs.jmedchem.1c00158, 9042-9055. doi: 10.1021/acs.jmedchem.1c00158

Effects of backbone cyclization on the pharmacokinetics and drug efficiency of the orally active analgesic conotoxin cVc1.1

Poth, Aaron G., Chiu, Francis C.K., Stalmans, Sofie, Hamilton, Brett R., Huang, Yen-Hua, Shackleford, David M., Patil, Rahul, Le, Thao T., Kan, Meng-Wei, Durek, Thomas, Wynendaele, Evelien, De Spiegeleer, Bart, Powell, Andrew K., Venter, Deon J., Clark, Richard J., Charman, Susan A. and Craik, David J. (2021). Effects of backbone cyclization on the pharmacokinetics and drug efficiency of the orally active analgesic conotoxin cVc1.1. Medicine in Drug Discovery, 10 100087, 1-10. doi: 10.1016/j.medidd.2021.100087

Advances in venom peptide drug discovery: where are we at and where are we heading?

Smallwood, Taylor B. and Clark, Richard J. (2021). Advances in venom peptide drug discovery: where are we at and where are we heading?. Expert Opinion on Drug Discovery, 16 (10) 17460441.2021.1922386, 1-11. doi: 10.1080/17460441.2021.1922386

A chameleonic macrocyclic peptide with drug delivery applications

Payne, Colton D., Franke, Bastian, Fisher, Mark F., Hajiaghaalipour, Fatemeh, McAleese, Courtney E., Song, Angela, Eliasson, Carl, Zhang, Jingjing, Jayasena, Achala S., Vadlamani, Grishma, Clark, Richard J., Minchin, Rodney F., Mylne, Joshua S. and Rosengren, K. Johan (2021). A chameleonic macrocyclic peptide with drug delivery applications. Chemical Science, 12 (19), 6670-6683. doi: 10.1039/d1sc00692d

ERK and mTORC1 inhibitors enhance the anti-cancer capacity of the octpep-1 venom-derived peptide in melanoma BRAF(V600E) mutations

Moral-Sanz, Javier, Fernandez-Rojo, Manuel A., Potriquet, Jeremy, Mukhopadhyay, Pamela, Brust, Andreas, Wilhelm, Patrick, Smallwood, Taylor B., Clark, Richard J., Fry, Bryan G., Alewood, Paul F., Waddell, Nicola, Miles, John J., Mulvenna, Jason P. and Ikonomopoulou, Maria P. (2021). ERK and mTORC1 inhibitors enhance the anti-cancer capacity of the octpep-1 venom-derived peptide in melanoma BRAF(V600E) mutations. Toxins, 13 (2) 146, 1-19. doi: 10.3390/toxins13020146

Pursuing orally bioavailable hepcidin analogues via cyclic N-methylated mini-hepcidins

Goncalves Monteiro, Daniela, van Dijk, Johannes W. A., Aliyanto, Randy, Fung, Eileen, Nemeth, Elizabeta, Ganz, Tomas, Rosengren, Johan and Clark, Richard J. (2021). Pursuing orally bioavailable hepcidin analogues via cyclic N-methylated mini-hepcidins. Biomedicines, 9 (2) 164, 1-14. doi: 10.3390/biomedicines9020164

The “C3aR Antagonist” SB290157 is a partial C5aR2 agonist

Li, Xaria X., Kumar, Vinod, Clark, Richard J., Lee, John D. and Woodruff, Trent M. (2021). The “C3aR Antagonist” SB290157 is a partial C5aR2 agonist. Frontiers in Pharmacology, 11 591398, 591398. doi: 10.3389/fphar.2020.591398

Chemical synthesis and characterisation of the complement C5 inhibitory peptide zilucoplan

Gorman, Declan M., Lee, John, Payne, Colton D., Woodruff, Trent M. and Clark, Richard J. (2021). Chemical synthesis and characterisation of the complement C5 inhibitory peptide zilucoplan. Amino Acids, 53 (1), 143-147. doi: 10.1007/s00726-020-02921-5

Defining the familial fold of the vicilin-buried peptide family

Payne, Colton D., Vadlamani, Grishma, Fisher, Mark F., Zhang, Jingjing, Clark, Richard J., Mylne, Joshua S. and Rosengren, K. Johan (2020). Defining the familial fold of the vicilin-buried peptide family. Journal of Natural Products, 83 (10), 3030-3040. doi: 10.1021/acs.jnatprod.0c00594

Exploring the use of helicogenic amino acids for optimising single chain relaxin-3 peptide agonists

Lee, Han Siean, Wang, Shu Hui, Daniel, James T., Hossain, Mohammed Akhter, Clark, Richard J., Bathgate, Ross A. D. and Rosengren, K. Johan (2020). Exploring the use of helicogenic amino acids for optimising single chain relaxin-3 peptide agonists. Biomedicines, 8 (10) 415, 1-16. doi: 10.3390/biomedicines8100415

Pharmacological characterisation of small molecule C5aR1 inhibitors in human cells reveals biased activities for signalling and function

Li, Xaria X., Lee, John D., Massey, Nicholas L., Guan, Carolyn, Robertson, Avril A. B., Clark, Richard J. and Woodruff, Trent M. (2020). Pharmacological characterisation of small molecule C5aR1 inhibitors in human cells reveals biased activities for signalling and function. Biochemical Pharmacology, 180 114156, 114156. doi: 10.1016/j.bcp.2020.114156

C5aR2 activation broadly modulates the signaling and function of primary human macrophages

Li, Xaria X., Clark, Richard J. and Woodruff, Trent M. (2020). C5aR2 activation broadly modulates the signaling and function of primary human macrophages. Journal of Immunology, 205 (4), 1-11. doi: 10.4049/jimmunol.2000407

Development of relaxin-3 agonists and antagonists based on grafted disulfide-stabilized scaffolds

Lee, Han Siean, Postan, Michael, Song, Angela, Clark, Richard J., Bathgate, Ross A. D., Haugaard-Kedström, Linda M. and Rosengren, K. Johan (2020). Development of relaxin-3 agonists and antagonists based on grafted disulfide-stabilized scaffolds. Frontiers in Chemistry, 8 87, 87. doi: 10.3389/fchem.2020.00087

Preclinical pharmacokinetics of complement C5a receptor antagonists PMX53 and PMX205 in mice

Kumar, Vinod, Lee, John D., Clark, Richard J., Noakes, Peter G., Taylor, Stephen M. and Woodruff, Trent M. (2020). Preclinical pharmacokinetics of complement C5a receptor antagonists PMX53 and PMX205 in mice. ACS Omega, 5 (5) acsomega.9b03735, 2345-2354. doi: 10.1021/acsomega.9b03735

Periplasmic expression of 4/7 alpha-conotoxin TxIA analogs in E. coli favors ribbon isomer formation suggestion of a binding mode at the α7 nAChR

El Hamdaoui, Yamina, Wu, Xiaosa, Clark, Richard J., Giribaldi, Julien, Anangi, Raveendra, Craik, David J., King, Glenn F., Dutertre, Sebastien, Kaas, Quentin, Herzig, Volker and Nicke, Annette (2019). Periplasmic expression of 4/7 alpha-conotoxin TxIA analogs in E. coli favors ribbon isomer formation suggestion of a binding mode at the α7 nAChR. Frontiers in Pharmacology, 10 (MAY) 577, 577. doi: 10.3389/fphar.2019.00577

Discovery of peptide probes to modulate oxytocin-type receptors of insects

Keov, Peter, Liutkevičiūtė, Zita, Hellinger, Roland, Clark, Richard J. and Gruber, Christian W. (2018). Discovery of peptide probes to modulate oxytocin-type receptors of insects. Scientific reports, 8 (1) 10020, 10020. doi: 10.1038/s41598-018-28380-3

Structure-activity studies reveal the molecular basis for GABAB-receptor mediated inhibition of high voltage-activated calcium channels by α-conotoxin Vc1.1

Sadeghi, Mahsa, Carstens, Bodil B., Callaghan, Brid P., Daniel, James T., Tae, Han Shen, O'Donnell, Tracey, Castro, Joel, Brierley, Stuart M., Adams, David J., Craik, David J. and Clark, Richard J. (2018). Structure-activity studies reveal the molecular basis for GABAB-receptor mediated inhibition of high voltage-activated calcium channels by α-conotoxin Vc1.1. ACS Chemical Biology, 13 (6), 1577-1587. doi: 10.1021/acschembio.8b00190

Development and validation of a LC-MS/MS assay for pharmacokinetic studies of complement C5a receptor antagonists PMX53 and PMX205 in mice

Kumar, Vinod, Lee, John D., Clark, Richard J. and Woodruff, Trent M. (2018). Development and validation of a LC-MS/MS assay for pharmacokinetic studies of complement C5a receptor antagonists PMX53 and PMX205 in mice. Scientific Reports, 8 (1) 8101, 8101. doi: 10.1038/s41598-018-26387-4

Peptide mimic for influenza vaccination using nonnatural combinatorial chemistry

Miles, John J., Tan, Mai Ping, Dolton, Garry, Edwards, Emily S. J., Galloway, Sarah A. E., Laugel, Bruno, Clement, Mathew, Makinde, Julia, Ladell, Kristin, Matthews, Katherine K., Watkins, Thomas S., Tungatt, Katie, Wong, Yide, Lee, Han Siean, Clark, Richard J., Pentier, Johanne M., Attaf, Meriem, Lissina, Anya, Ager, Ann, Gallimore, Awen, Rizkallah, Pierre J., Gras, Stephanie, Rossjohn, Jamie, Burrows, Scott R., Cole, David K., Price, David A. and Sewell, Andrew K. (2018). Peptide mimic for influenza vaccination using nonnatural combinatorial chemistry. Journal of Clinical Investigation, 128 (4), 1569-1580. doi: 10.1172/JCI91512

Transcriptome and toxin family analysis of the paralysis tick, Ixodes holocyclus

Rodriguez-Valle, Manuel, Moolhuijzen, Paula, Barrero, Roberto A., Ong, Chian Teng, Busch, Greta, Karbanowicz, Thomas, Booth, Mitchell, Clark, Richard, Koehbach, Johannes, Ijaz, Hina, Broady, Kevin, Agnew, Kim, Knowles, Aleta G., Bellgard, Matthew I. and Tabor, Ala E. (2018). Transcriptome and toxin family analysis of the paralysis tick, Ixodes holocyclus. International Journal for Parasitology, 48 (1), 71-82. doi: 10.1016/j.ijpara.2017.07.007

G-Protein Coupled Receptors Targeted by Analgesic Venom Peptides

Daniel, James T. and Clark, Richard J. (2017). G-Protein Coupled Receptors Targeted by Analgesic Venom Peptides. Toxins, 9 (11) 372, 1-26. doi: 10.3390/toxins9110372

Helminth immunomodulation in autoimmune disease

Smallwood, Taylor B., Giacomin, Paul R., Loukas, Alex, Mulvenna, Jason P., Clark, Richard J. and Miles, John J. (2017). Helminth immunomodulation in autoimmune disease. Frontiers in Immunology, 8 (APR) 453, 453. doi: 10.3389/fimmu.2017.00453

Effects of linker sequence modifications on the structure, stability and biological activity of a cyclic α-conotoxin

Carstens, Bodil B., Swedberg, Joakim, Berecki, Geza, Adams, David J., Craik, David J. and Clark, Richard J. (2016). Effects of linker sequence modifications on the structure, stability and biological activity of a cyclic α-conotoxin. Biopolymers, 106 (6), 864-875. doi: 10.1002/bip.22848

Unveiling the diversity of cyclotides by combining peptidome and transcriptome analysis

Koehbach, Johannes and Clark, Richard J. (2016). Unveiling the diversity of cyclotides by combining peptidome and transcriptome analysis. Biopolymers, 106 (6), 774-783. doi: 10.1002/bip.22858

Discovery of functionally selective C5aR2 ligands: novel modulators of C5a signalling

Croker, Daniel E., Monk, Peter N., Halai, Reena, Kaeslin, Geraldine, Schofield, Zoe, Wu, Mike C. L., Clark, Richard J., Blaskovich, Mark A. T., Morikis, Dimitrios, Floudas, Christodoulos A., Cooper, Matthew and Woodruff, Trent M. (2016). Discovery of functionally selective C5aR2 ligands: novel modulators of C5a signalling. Immunology and Cell Biology, 94 (8), 787-795. doi: 10.1038/icb.2016.43

Release of bioactive peptides from polyurethane films in vitro and in vivo: Effect of polymer composition

Zhang, Jing, Woodruff, Trent M., Clark, Richard J., Martin, Darren J. and Minchin, Rodney F. (2016). Release of bioactive peptides from polyurethane films in vitro and in vivo: Effect of polymer composition. Acta Biomaterialia, 41, 264-272. doi: 10.1016/j.actbio.2016.05.034

The N-terminal pro-domain of the kalata B1 cyclotide precursor is intrinsically unstructured

Daly, Norelle L., Gunasekera, Sunithi, Clark, Richard J., Lin, Feng, Wade, John D., Anderson, Marilyn and Craik, David J. (2016). The N-terminal pro-domain of the kalata B1 cyclotide precursor is intrinsically unstructured. Biopolymers, 106 (6), 825-833. doi: 10.1002/bip.22977

Tick holocyclotoxins trigger host paralysis by presynaptic inhibition

Chand, Kirat K., Lee, Kah Meng, Lavidis, Nickolas A., Rodriguez-Valle, Manuel, Ijaz, Hina, Koehbach, Johannes, Clark, Richard J., Lew-Tabor, Ala and Noakes, Peter G. (2016). Tick holocyclotoxins trigger host paralysis by presynaptic inhibition. Scientific Reports, 6 (1) 29446, 29446. doi: 10.1038/srep29446

Structure-Activity Studies of Cysteine-Rich α-Conotoxins that Inhibit High-Voltage-Activated Calcium Channels via GABAB Receptor Activation Reveal a Minimal Functional Motif

Carstens, Bodil B., Berecki, Géza, Daniel, James T., Lee, Han Siean, Jackson, Kathryn A. V., Tae, Han-Shen, Sadeghi, Mahsa, Castro, Joel, O'Donnell, Tracy, Deiteren, Annemie, Brierley, Stuart M., Craik, David J., Adams, David J. and Clark, Richard J. (2016). Structure-Activity Studies of Cysteine-Rich α-Conotoxins that Inhibit High-Voltage-Activated Calcium Channels via GABAB Receptor Activation Reveal a Minimal Functional Motif. Angewandte Chemie, 128 (15), 4770-4774. doi: 10.1002/ange.201600297

Structure-Activity Studies of Cysteine-Rich α-Conotoxins that Inhibit High Voltage-Activated Calcium Channels via GABAB Receptor Activation Reveal a Minimal Functional Motif

Carstens, Bodil B., Berecki, Geza, Daniel, James T., Lee, Han Siean, Jackson, Kathryn A. V., Tae, Han-Shen, Sadeghi, Mahsa, Castro, Joel, O'Donnell, Tracy, Deiteren, Annemie, Brierley, Stuart M., Craik, David J., Adams, David J. and Clark, Richard J. (2016). Structure-Activity Studies of Cysteine-Rich α-Conotoxins that Inhibit High Voltage-Activated Calcium Channels via GABAB Receptor Activation Reveal a Minimal Functional Motif. Angewandte Chemie - International Edition, 55 (15), 4692-4696. doi: 10.1002/anie.201600297

Transforming conotoxins into cyclotides: backbone cyclization of P-superfamily conotoxins

Akcan, Muharrem, Clark, Richard J., Daly, Norelle L., Conibear, Anne C., de Faoite, Andrew, Heghinian, Mari D., Sahil, Talwar, Adams, David J., Mari, Frank and Craik, David J. (2015). Transforming conotoxins into cyclotides: backbone cyclization of P-superfamily conotoxins. Peptide Science, 104 (6), 682-692. doi: 10.1002/bip.22699

Isolation, characterization, and synthesis of the barrettides: disulfide-containing peptides from the marine sponge Geodia barretti

Carstens, Bodil B., Rosengren, K. Johan, Gunasekera, Sunithi, Schempp, Stefanie, Bohlin, Lars, Dahlstrom, Mia, Clark, Richard J. and Goransson, Ulf (2015). Isolation, characterization, and synthesis of the barrettides: disulfide-containing peptides from the marine sponge Geodia barretti. Journal of Natural Products, 78 (8), 1886-1893. doi: 10.1021/acs.jnatprod.5b00210

Inhibition of human prolyl oligopeptidase activity by the cyclotide psysol 2 isolated from Psychotria solitudinum

Hellinger, Roland, Koehbach, Johannes, Puigpinos, Albert, Clark, Richard J., Tarrago, Teresa, Giralt, Ernest and Gruber, Christian W. (2015). Inhibition of human prolyl oligopeptidase activity by the cyclotide psysol 2 isolated from Psychotria solitudinum. Journal of Natural Products, 78 (5), 1073-1082. doi: 10.1021/np501061t

In vivo efficacy of anuran rypsin inhibitory peptides against Staphylococcal Skin infection and the impact of peptide cyclization

Malik, U., Silva, O. N., Fensterseifer, I. C. M., Chan, A., Clark, R. J., Franco, O. L., Daly, N. L. and Craik, D. J. (2015). In vivo efficacy of anuran rypsin inhibitory peptides against Staphylococcal Skin infection and the impact of peptide cyclization. Antimicrobial Agents and Chemotherapy, 59 (4), 2113-2121. doi: 10.1128/AAC.04324-14

Alanine scan ofα-conotoxin regIIA reveals a selective α3β4 nicotinic acetylcholine receptor antagonist

Kompella, Shiva N., Hung, Andrew, Clark, Richard J., Mari, Frank and Adams, David J. (2015). Alanine scan ofα-conotoxin regIIA reveals a selective α3β4 nicotinic acetylcholine receptor antagonist. Journal of Biological Chemistry, 290 (2), 1039-1048. doi: 10.1074/jbc.M114.605592

Differential Cav2.1 and Cav2.3 channel inhibition by baclofen and α-conotoxin Vc1.1 via GABAB receptor activation

Berecki, Géza, McArthur, Jeffrey R., Cuny, Hartmut, Clark, Richard J. and Adams, David J. (2014). Differential Cav2.1 and Cav2.3 channel inhibition by baclofen and α-conotoxin Vc1.1 via GABAB receptor activation. Journal of General Physiology, 143 (4), 465-479. doi: 10.1085/jgp.201311104

Erratum: Chemical re-engineering of chlorotoxin improves bioconjugation properties for tumor imaging and targeted therapy (Journal of Medicinal Chemistry (2011) 54 (782-787) DOI: 10.1021/jm101018r)

Akcan, Muharrem, Stroud, Mark R., Hansen, Stacey J., Clark, Richard J., Daly, Norelle L., Craik, David J. and Olson, James M. (2013). Erratum: Chemical re-engineering of chlorotoxin improves bioconjugation properties for tumor imaging and targeted therapy (Journal of Medicinal Chemistry (2011) 54 (782-787) DOI: 10.1021/jm101018r). Journal of Medicinal Chemistry, 56 (23), 9807-9807. doi: 10.1021/jm4016119

Identifying key amino acid residues that affect alpha-conotoxin AuIB inhibition of alpha3beta4 nicotinic acetylcholine receptors

Grishin, Anton A., Cuny, Hartmut, Hung, Andrew, Clark, Richard J., Brust, Andreas, Akondi, Kalyana, Alewood, Paul F., Craik, David J. and Adams, David J. (2013). Identifying key amino acid residues that affect alpha-conotoxin AuIB inhibition of alpha3beta4 nicotinic acetylcholine receptors. Journal of Biological Chemistry, 288 (48), 34428-34442. doi: 10.1074/jbc.M113.512582

Novel inhibitor cystine knot peptides from momordica charantia

He, Wen-Jun, Chan, Lai Yue, Clark, Richard J., Tang, Jun, Zeng, Guang-Zhi, Franco, Octavio L., Cantacessi, Cinzia, Craik, David J., Daly, Norelle L. and Tan, Ning-Hua (2013). Novel inhibitor cystine knot peptides from momordica charantia. PLoS ONE, 8 (10) e75334, e75334.1-e75334.10. doi: 10.1371/journal.pone.0075334

Design, synthesis and characterisation of cyclic analogues of the iron regulatory peptide hormone hepcidin

Clark, Richard J., Preza, Gloria C., Tan, Chia Chia, van Dijk, Johannes W. A., Fung, Eileen, Nemeth, Elizabeta, Ganz, Tomas and Craik, David J. (2013). Design, synthesis and characterisation of cyclic analogues of the iron regulatory peptide hormone hepcidin. Biopolymers: Peptide Science, 100 (5), 519-526. doi: 10.1002/bip.22350

Cyclization of conotoxins to improve their biopharmaceutical properties

Clark, Richard J., Akcan, Muharrem, Kaas, Quentin, Daly, Norelle L. and Craik, David J. (2012). Cyclization of conotoxins to improve their biopharmaceutical properties. Toxicon, 59 (4), 446-455. doi: 10.1016/j.toxicon.2010.12.003

Corrigendum: Engineering of Conotoxins for the Treatment of Pain (Current Pharmaceutical Design, (2011), 17, 38)

Carstens, Bodil B., Clark, Richard J., Daly, Norelle L., Harvey, Peta J., Kaas, Quentin and Craik, David J. (2012). Corrigendum: Engineering of Conotoxins for the Treatment of Pain (Current Pharmaceutical Design, (2011), 17, 38). Current Pharmaceutical Design, 18 (8) doi: 10.2174/138161212799315858

Engineering cyclic peptide toxins

Clark, Richard J. and Craik, David J. (2012). Engineering cyclic peptide toxins. Methods in Enzymology, 503, 57-74. doi: 10.1016/B978-0-12-396962-0.00003-3

Engineering of conotoxins for the treatment of pain

Carstens, Bodil B., Clark, Richard J., Daly, Norelle L., Harvey, Peta J., Kaas, Quentin and Craik, David J. (2011). Engineering of conotoxins for the treatment of pain. Current Pharmaceutical Design, 17 (38), 4242-4253. doi: 10.2174/138161211798999401

Engineering pro-angiogenic peptides using stable disulfide-rich cyclic scaffolds

Chan, Lai Y., Gunasekera, Sunithi, Henriques, Sonia T., Worth, Natalie F., Le, Sarah-Jane, Clark, Richard J., Campbell, Julie H., Craik, David J. and Daly, Norelle L. (2011). Engineering pro-angiogenic peptides using stable disulfide-rich cyclic scaffolds. Blood, 118 (25), 6709-6717. doi: 10.1182/blood-2011-06-359141

Effects of cyclization on stability, structure, and activity of alpha-conotoxin RgIA at the alpha 9 alpha 10 nicotinic acetylcholine receptor and GABA(B) receptor

Halai, Reena, Callaghan, Brid, Daly, Norelle L., Clark, Richard J., Adams, David J. and Craik, David J. (2011). Effects of cyclization on stability, structure, and activity of alpha-conotoxin RgIA at the alpha 9 alpha 10 nicotinic acetylcholine receptor and GABA(B) receptor. Journal of Medicinal Chemistry, 54 (19), 6984-6992. doi: 10.1021/jm201060r

The role of conserved Glu residue on cyclotide stability and activity: A structural and functional study of kalata B12, a naturally occurring Glu to Asp mutant

Wang, Conan K. L., Clark, Richard J., Harvey, Peta J., Rosengren, K. Johan, Cemazar, Masa and Craik, David J. (2011). The role of conserved Glu residue on cyclotide stability and activity: A structural and functional study of kalata B12, a naturally occurring Glu to Asp mutant. Biochemistry, 50 (19), 4077-4086. doi: 10.1021/bi2004153

Design, synthesis, and characterization of a single-chain peptide antagonist for the relaxin-3 receptor RXFP3

Haugaard-Kedström, Linda M., Shabanpoor, Fazel, Hossain, Mohammed Akhter, Clark, Richard J., Ryan, Philip J., Craik, David J., Gundlach, Andrew L., Wade, John D., Bathgate, Ross A. D. and Rosengren, K. Johan (2011). Design, synthesis, and characterization of a single-chain peptide antagonist for the relaxin-3 receptor RXFP3. Journal of the American Chemical Society, 133 (13), 4965-4974. doi: 10.1021/ja110567j

Structure and activity of alpha-conotoxin PeIA at nicotinic acetylcholine receptor subtypes and GABA(B) receptor-coupled N-type calcium channels

Daly, Norelle L., Callaghan, Brid, Clark, Richard J., Nevin, Simon T., Adams, David J. and Craik, David J. (2011). Structure and activity of alpha-conotoxin PeIA at nicotinic acetylcholine receptor subtypes and GABA(B) receptor-coupled N-type calcium channels. Journal of Biological Chemistry, 286 (12), 10233-10237. doi: 10.1074/jbc.M110.196170

Understanding the structure/activity relationships of the iron regulatory peptide hepcidin

Clark, Richard J., Tan, Chia Chia, Preza, Gloria C., Nemeth, Elizabeta, Ganz, Tomas and Craik, David J. (2011). Understanding the structure/activity relationships of the iron regulatory peptide hepcidin. Chemistry and Biology, 18 (3), 336-343. doi: 10.1016/j.chembiol.2010.12.009

Chemical re-engineering of chlorotoxin improves bioconjugation properties for tumor imaging and targeted therapy

Akcan, M, Stroud, MR, Hansen, SJ, Clark, RJ, Daly, NL, Craik, DJ and Olson, JM (2011). Chemical re-engineering of chlorotoxin improves bioconjugation properties for tumor imaging and targeted therapy. Journal of Medicinal Chemistry, 54 (3), 782-787. doi: 10.1021/jm101018r

Interlocking Disulfides in Circular Proteins: Toward Efficient Oxidative Folding of Cyclotides

Aboye, Teshome Leta, Clark, Richard J., Burman, Robert, Roig, Marta Bajona, Craik, David J. and Goransson, Ulf (2011). Interlocking Disulfides in Circular Proteins: Toward Efficient Oxidative Folding of Cyclotides. Antioxidants & Redox Signaling, 14 (1), 77-86. doi: 10.1089/ars.2010.3112

Stabilization of α-conotoxin AuIB: Influences of disulfide connectivity and backbone cyclization

Lovelace, Erica S., Gunasekera, Sunithi, Alvarmo, Charlotta, Clark, Richard J., Nevin, Simon T., Grishin, Anton A., Adams, David J., Craik, David J. and Daly, Norelle L. (2011). Stabilization of α-conotoxin AuIB: Influences of disulfide connectivity and backbone cyclization. Antioxidants and Redox Signaling, 14 (1), 87-95. doi: 10.1089/ars.2009.3068

Structural and functional analysis of human liver-expressed antimicrobial peptide 2

Henriques, Sonia Troeira, Tan, Chia Chia, Craik, David J. and Clark, Richard J. (2010). Structural and functional analysis of human liver-expressed antimicrobial peptide 2. ChemBioChem, 11 (15), 2148-2157. doi: 10.1002/cbic.201000400

Isolation, sequencing, and structure - Activity relationships of cyclotides

Ireland, David C., Clark, Richard J., Daly, Norelle L. and Craik, David J. (2010). Isolation, sequencing, and structure - Activity relationships of cyclotides. Journal of Natural Products, 73 (9), 1610-1622. doi: 10.1021/np1000413

The engineering of an orally active conotoxin for the treatment of neuropathic pain

Clark, Richard J., Jensen, Jonas, Nevin, Simon T., Callaghan, Brid P., Adams, David J. and Craik, David J. (2010). The engineering of an orally active conotoxin for the treatment of neuropathic pain. Angewandte Chemie (International Edition), 49 (37), 6545-6548. doi: 10.1002/anie.201000620

Lysine-scanning mutagenesis reveals an amendable face of the cyclotide kalata B1 for the optimization of nematocidal activity

Huang, Yen-Hua (黃彥華), Colgrave, Michelle L., Clark, Richard J., Kotze, Andrew C. and Craik, David J. (2010). Lysine-scanning mutagenesis reveals an amendable face of the cyclotide kalata B1 for the optimization of nematocidal activity. Journal of Biological Chemistry, 285 (14), 10797-10805. doi: 10.1074/jbc.M109.089854

Native chemical ligation applied to the synthesis and bioengineering of circular peptides and proteins

Clark, Richard J. and Craik, David J. (2010). Native chemical ligation applied to the synthesis and bioengineering of circular peptides and proteins. Biopolymers, 94 (4), 414-422. doi: 10.1002/bip.21372

Beta-arrestin 2 is required for complement C1q expression in macrophages and constrains factor-independent survival

Lattin, J. E., Greenwood, K. P., Daly, N. L., Kelly, G, Zidar, D. A., Clark, R. J., Thomas, W. G., Kellie, Stuart, Craik, D. J., Hume, D. A. and Sweet, M. J. (2009). Beta-arrestin 2 is required for complement C1q expression in macrophages and constrains factor-independent survival. Molecular Immunology, 47 (2-3), 340-347. doi: 10.1016/j.molimm.2009.09.012

A synthetic combinatorial strategy for developing alpha-conotoxin analogs as potent alpha7 nicotinic acetylcholine receptor antagonists

Armishaw, Christopher J., Singh, Narender, Medina-Franco, Jose L., Clark, Richard J., Scott, Krystle C. M., Houghten, Richard A. and Jensen, Anders A. (2009). A synthetic combinatorial strategy for developing alpha-conotoxin analogs as potent alpha7 nicotinic acetylcholine receptor antagonists. Journal of Biological Chemistry, 285 (3), 1809-1821. doi: 10.1074/jbc.M109.071183

Scanning mutagenesis of alpha-conotoxin Vc1.1 reveals residues crucial for activity at the alpha-9 alpha-10 nicotinic acetylcholine receptor

Halai, Reena, Clark, Richard J., Nevin, Simon T., Jensen, Jonas E., Adams, David J. and Craik, David J. (2009). Scanning mutagenesis of alpha-conotoxin Vc1.1 reveals residues crucial for activity at the alpha-9 alpha-10 nicotinic acetylcholine receptor. Journal of Biological Chemistry, 284 (30), 20275-20284. doi: 10.1074/jbc.M109.015339

Dissecting the oxidative folding of circular cystine knot miniproteins

Gunasekera, Sunithi, Daly, Norelle L., Clark, Richard J. and Craik, David J. (2009). Dissecting the oxidative folding of circular cystine knot miniproteins. Antioxidants and Redox Signalling, 11 (5), 971-980. doi: 10.1089/ars.2008.2295

Rational design of alpha-conotoxin analogues targeting alpha 7 nicotinic acetylcholine receptors: Improved antagonistic activity by incorporation of proline derivatives

Armishaw, Christopher, Jensen, Anders A., Balle, Thomas, Clark, Richard J., Harpsøe, Kasper, Skonberg, Christian, Liljefors, Tommy and Strømgaard, Kristian (2009). Rational design of alpha-conotoxin analogues targeting alpha 7 nicotinic acetylcholine receptors: Improved antagonistic activity by incorporation of proline derivatives. Journal of Biological Chemistry, 284 (14), 9498-9512. doi: 10.1074/jbc.M806136200

Inhibition of neuronal nicotinic acetylcholine receptor subtypes by alpha-conotoxin GID and analogues

Millard, Emma L., Nevin, Simon T., Loughnan, Marion L., Nicke, Annette, Clark, Richard J., Alewood, Paul F., Lewis, Richard J., Adams, David J., Craik, David J. and Daly, Norelle L. (2009). Inhibition of neuronal nicotinic acetylcholine receptor subtypes by alpha-conotoxin GID and analogues. Journal of Biological Chemistry, 284 (8), 4944-4951. doi: 10.1074/jbc.M804950200

Analgesic alpha-Conotoxins Vc1.1 and Rg1A inhibit N-type calcium channels in rat sensory neurons via GABA-B receptor activation

Callaghan, Brid, Haythornthwaite, Alison R., Berecki, Geza, Clark, Richard J., Craik, David J. and Adams, David J. (2008). Analgesic alpha-Conotoxins Vc1.1 and Rg1A inhibit N-type calcium channels in rat sensory neurons via GABA-B receptor activation. The Journal of Neuroscience, 28 (43), 10943-10951. doi: 10.1523/JNEUROSCI.3594-08.2008

Engineering stablized vascular endothelial growth factor-A antagonists: synthesis, structural characterization and bioactivity of grafted analogues of cyclotides

Gunasekera, Sunithi, Foley, Fiona M., Clark, Richard J., Sando, Lillian, Fabri, Louis J., Craik, David J. and Daly, Norelle L. (2008). Engineering stablized vascular endothelial growth factor-A antagonists: synthesis, structural characterization and bioactivity of grafted analogues of cyclotides. Journal of Medicinal Chemistry, 51 (24), 7697-7704. doi: 10.1021/jm800704e

Binding mode of alpha-conotoxins to an acetylcholine binding protein determined by saturation transfer difference NMR

Westermann, Jan, Clark, Richard J. and Craik, David J. (2008). Binding mode of alpha-conotoxins to an acetylcholine binding protein determined by saturation transfer difference NMR. Protein And Peptide Letters, 15 (9), 910-914. doi: 10.2174/092986608785849335

The three-dimensional structure of the analgesic alpha-conotoxin, RgIA

Clark, Richard J., Daly, Norelle L., Halai, Reena, Nevin, Simon T., Adams, David J. and Craik, David J. (2008). The three-dimensional structure of the analgesic alpha-conotoxin, RgIA. FEBS Letters, 582 (5), 597-602. doi: 10.1016/j.febslet.2008.01.027

The alpine violet, Viola biflora, is a rich source of cyclotides with potent cytotoxicity

Herrman, Anders, Burman, Robert, Mylne, Joshua S., Karlsson, Gustav, Gullbo, Joachim, Craik, David J., Clark, Richard J. and Goransson, Ulf (2008). The alpine violet, Viola biflora, is a rich source of cyclotides with potent cytotoxicity. Phytochemistry, 69 (4), 939-952. doi: 10.1016/j.phytochem.2007.10.023

Cyclotides and conotoxins ultra-stable disulfide-rich peptides

Craik, David J., Clark, Richard J. and Daly, Norelle L. (2008). Cyclotides and conotoxins ultra-stable disulfide-rich peptides. Chimica Oggi -Chemistry Today, 26 (4), 20-22.

Utlra-stable peptide scaffolds for protein engineering synthesis and folding of the circular cystine knotted cyclotide cycloviolacin O2

Aboye, T, Clark, Richard J., Craik, David J. and Goransson, U (2008). Utlra-stable peptide scaffolds for protein engineering synthesis and folding of the circular cystine knotted cyclotide cycloviolacin O2. ChemBioChem, 9 (1), 103-113. doi: 10.1002/cbic.200700357

Are alpha 9 alpha 10 nicotinic acetylcholine receptors a pain target for alpha-conotoxins?

Nevin, Simon T., Clark, Richard J., Klimis, H., Christie, M. J., Craik, David J. and Adams, David J. (2007). Are alpha 9 alpha 10 nicotinic acetylcholine receptors a pain target for alpha-conotoxins?. Molecular Pharmacology, 72 (6), 1406-1410. doi: 10.1124/mol.107.040568

Structure of alpha-conotoxin BuIA: Influences of disulfide connectivity on structural dynamics

Jin, Ai-Hua, Brandstaetter, Hemma, Nevin, Simon T., Tan, Chia Chia, Clark, Richard J., Adams, David J., Alewood, Paul F., Craik, David J. and Daly, Norelle L. (2007). Structure of alpha-conotoxin BuIA: Influences of disulfide connectivity on structural dynamics. BMC Structural Biology, 7 (28) 28, 1-13. doi: 10.1186/1472-6807-7-28

A novel plant protein-disulfide isomerase involved in the oxidative folding of cystine knot defense proteins

Gruber, C. W, Cemazar, M., Clark, R. .J, Horibe, T, Renda, R. F., Anderson, M. A. and Craik, D. J. (2007). A novel plant protein-disulfide isomerase involved in the oxidative folding of cystine knot defense proteins. Journal of Biological Chemistry, 282 (28), 20435-20446. doi: 10.1074/jbc.M700018200

Potential therapeutic applications of the cyclotides and related cystine knot mini-proteins

Craik, D. J, Clark, R. J and Daly, N. L (2007). Potential therapeutic applications of the cyclotides and related cystine knot mini-proteins. Expert Opinion On Investigational Drugs, 16 (5), 595-604. doi: 10.1517/13543784.16.5.595

The conserved N-terminal repeat of the plant cyclotides does not assist folding of cyclotide precursor in vitro

Gunasekera, S, Daly, NL, Clark, RJ, Simonsen, SM, Foley, FM, Saska, I, Lin, F, Wade, JD, Anderson, MA and Craik, DJ (2007). The conserved N-terminal repeat of the plant cyclotides does not assist folding of cyclotide precursor in vitro. Biopolymers, 88 (4), 540-540.

The cyclotide fingerprint in Oldenlandia affinis: Elucidation of chemically modified, linear and novel macrocyclic peptides

Plan, M. R. R, Goransson, U, Clark, R. J, Daly, N. L, Colgrave, M. L and Craik, D.J (2007). The cyclotide fingerprint in Oldenlandia affinis: Elucidation of chemically modified, linear and novel macrocyclic peptides. Chembiochem, 8 (9), 1001-1011. doi: 10.1002/cbic.200700097

The Synthesis, Structural Characterization, and Receptor Specificity of the {alpha}-Conotoxin Vc1.1

Clark, Richard J., Fischer, Harald, Nevin, Simon T., Adams, David J. and Craik, David J. (2006). The Synthesis, Structural Characterization, and Receptor Specificity of the {alpha}-Conotoxin Vc1.1. Journal of Biological Chemistry, 281 (32), 23254-23263. doi: 10.1074/jbc.M604550200

Kalata B8, a novel antiviral circular protein, exhibits conformational flexibility in the cystine knot motif

Daly, N. L., Clark, R. J., Plan, M. R. and Craik, D. J. (2006). Kalata B8, a novel antiviral circular protein, exhibits conformational flexibility in the cystine knot motif. Biochemical Journal, 393 (Part 3), 619-626. doi: 10.1042/BJ20051371

Structural plasticity of the cyclic-cystine-knot framework: implications for biological activity and drug design

Clark, R. J., Daly, N. L. and Craik, D. J. (2006). Structural plasticity of the cyclic-cystine-knot framework: implications for biological activity and drug design. Biochemical Journal, 394 (Part 1), 85-93. doi: 10.1042/BJ20051691

The absolute structural requirement for a proline in the P3 '-position of Bowman-Birk protease inhibitors is surmounted in the minimized SFTI-1 scaffold

Daly, N. L., Chen, Y. K., Foley, F. M., Bansal, P. S., Bharathi, R., Clark, R. J., Sommerhoff, C. P. and Craik, D. J. (2006). The absolute structural requirement for a proline in the P3 '-position of Bowman-Birk protease inhibitors is surmounted in the minimized SFTI-1 scaffold. Journal of Biological Chemistry, 281 (33), 23668-23675. doi: 10.1074/jbc.M601426200

Disulfide bond mutagenesis and the structure and function of the head-to-tail macrocyclic trypsin inhibitor SFTI-1

Korsinczky, M. L. J., Clark, R. J. and Craik, D. J. (2005). Disulfide bond mutagenesis and the structure and function of the head-to-tail macrocyclic trypsin inhibitor SFTI-1. Biochemistry, 44 (4), 1145-1153. doi: 10.1021/bi048297r

Engineering stable peptide toxins by means of backbone cyclization: Stabilization of the alpha-conotoxin MII

Clark, R. J., Fischer, H., Dempster, L., Daly, N. L., Rosengren, K. J., Nevin, S. T., Meunier, F. A., Adams, D. J. and Craik, D. J. (2005). Engineering stable peptide toxins by means of backbone cyclization: Stabilization of the alpha-conotoxin MII. Proceedings of The National Academy of Sciences of The United States of America, 102 (39), 13767-13772. doi: 10.1073/pnas.0504613102

Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins

Dutton, J. L., Renda, R. F., Waine, C., Clark, R. J., Daly, N. L., Jennings, C. V., Anderson, M. A. and Craik, D. J. (2004). Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins. Journal of Biological Chemistry, 279 (45), 46858-46867. doi: 10.1074/jbc.M407421200

Disulfide folding pathways of cystine knot proteins: Tying the knot within the circular backbone of the cyclotides

Daly, N. L., Clark, R. J. and Craik, D. J. (2003). Disulfide folding pathways of cystine knot proteins: Tying the knot within the circular backbone of the cyclotides. The Journal of Biological Chemistry, 278 (8), 6314-6322. doi: 10.1074/jbc.M210492200

Diversity in the disulfide folding pathways of cystine knot peptides

Daly, N. L., Clark, R. J., Goransson, U. and Craik, D. J. (2003). Diversity in the disulfide folding pathways of cystine knot peptides. Letters in Peptide Science, 10 (5-6), 523-531. doi: 10.1007/BF02442584

Linearization of a naturally occurring circular protein maintains structure but eliminates hemolytic activity

Barry, D. G., Daly, N. L., Clark, R. J., Sando, L. and Craik, D. J. (2003). Linearization of a naturally occurring circular protein maintains structure but eliminates hemolytic activity. Biochemistry, 42 (22), 6688-6695. doi: 10.1021/bi027323n

Microcin J25 has a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone

Rosengren, K. J., Clark, R. J., Daly, N. L., Goransson, U., Jones, A. and Craik, D. J. (2003). Microcin J25 has a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone. Journal of the American Chemical Society, 125 (41), 12464-12474. doi: 10.1021/JA0367703

Structure-function studies of the plant cyclotides: The role of a circular protein backbone

Craik, David J., Barry, Daniel G., Clark, Richard J., Daly, Norelle L. and Sando, Lillian (2003). Structure-function studies of the plant cyclotides: The role of a circular protein backbone. Toxin Reviews, 22 (4), 555-576. doi: 10.1081/TXR-120026914

Structure-function studies of the plant cyclotides: the role of a circular protein backbone

Craik, David J., Barry, Daniel G., Clark, Richard J., Daly, Norelle L. and Sando, Lillian (2003). Structure-function studies of the plant cyclotides: the role of a circular protein backbone. Journal of Toxicology: Toxin Reviews, 22 (4), 555-576. doi: 10.1081/TXR-120026914

A sponge allelochemical induces ascidian settlement but inhibits metamorphosis

Green, K. M., Russell, B. D., Clark, R. J., Jones, M. K ., Garson, M. J., Skilleter, G. A. and Degnan, B. M. (2002). A sponge allelochemical induces ascidian settlement but inhibits metamorphosis. Marine Biology, 140 (2), 355-363. doi: 10.1007/s002270100698

Discovery and structures of the cyclotides: novel macrocyclic peptides from plants

Craik, David J., Anderson, Marilyn A., Barry, Daniel G., Clark, Richard J., Daly, Norelle L., Jennings, Cameron V. and Mulvenna, Jason P. (2001). Discovery and structures of the cyclotides: novel macrocyclic peptides from plants. Letters in Peptide Science, 8 (3-5), 119-128. doi: 10.1007/BF02446507

Antifungal alkyl amino alcohols from the tropical marine sponge Haliclona n. sp.

Clark, R. J., Garson, M. J. and Hooper, J. N. A. (2001). Antifungal alkyl amino alcohols from the tropical marine sponge Haliclona n. sp.. Journal of Natural Products, 64 (12), 1568-1571. doi: 10.1021/np010246x

Vinylfurans revisited: A new sesquiterpene from Euryspongia deliculata (vol 62, pg 915, 1999)

Clark, Richard J., Garson, Mary J., Brereton, Ian M. and Kennedy, John A. (2000). Vinylfurans revisited: A new sesquiterpene from Euryspongia deliculata (vol 62, pg 915, 1999). Journal of Natural Products, 63 (7), 1046-1046. doi: 10.1021/np0001934

New Isocyano and Isothiocyanato Terpene Metabolites from the Tropical Marine Sponge Acanthella cavernosa

Clark, Richard J., Stapleton, Bronwin L. and Garson, Mary J. (2000). New Isocyano and Isothiocyanato Terpene Metabolites from the Tropical Marine Sponge Acanthella cavernosa. Tetrahedron, 56 (19), 3071-3076. doi: 10.1016/S0040-4020(00)00226-X

Vinylfurans revisited: A new sesquiterpene from Euryspongia deliculata

Clark, R. J., Garson, M. J., Brereton, I. M. and Kennedy, J. A. (1999). Vinylfurans revisited: A new sesquiterpene from Euryspongia deliculata. Journal of Natural Products, 62 (6), 915-916. doi: 10.1021/np980557g

The haliclonacyclamines, cytotoxic tertiary alkaloids from the tropical marine sponge Haliclona sp

Clark, Richard J., Field, Kim L., Charan, Romila D., Garson, Mary J., Brereton, Ian M. and Willis, Anthony C. (1998). The haliclonacyclamines, cytotoxic tertiary alkaloids from the tropical marine sponge Haliclona sp. Tetrahedron, 54 (30), 8811-8826. doi: 10.1016/S0040-4020(98)00473-6

WS1.4: Biostimulation of bacteria for streamlined identification of new antimicrobial compounds

Mirzaee, Hooman, Ariens, Emily, Booth, James, Carvalhais, Lilia C., Blaskovich, Mark A.T., Clark, Richard J. and Schenk, Peer M. (2022). WS1.4: Biostimulation of bacteria for streamlined identification of new antimicrobial compounds. 32nd International Congress of Antimicrobial Chemotherapy (ICC), Perth, WA Australia, 27 – 30 November 2022. Amsterdam, Netherlands: Elsevier BV. doi: 10.1016/s2213-7165(22)00272-7

The antiproliferative profile of a linear octopus-derived peptide in melanoma of BRAF-mutation

Fernandez-Rojo, Manuel A., Brust, Andreas, Potriquet, Jeremy, Daley, Joshua, Ragnarsson, Lotten, Andersson, Asa, Mukhopadhyay, Pamela, Wilhelm, Patrick, Chin, Yanni, Smallwood, Taylor, Clark, Richard, King, Glenn, Ramm, Grant, Waddell, Nic, Lewis, Richard, Boyle, Glen, Fry, Bryan, Alewood, Paul, Mulvenna, Jason, Miles, John and Ikonomopoulou, Maria P. (2020). The antiproliferative profile of a linear octopus-derived peptide in melanoma of BRAF-mutation. 20th World Congress of the International Society on Toxinology, Buenos Aires, Argentina, 8-13 September 2019. Oxford, United Kingdom: Elsevier. doi: 10.1016/j.toxicon.2019.10.014

Motor neuron disease proteins activate complement and generate C5a

Deora, Vandana, Mantovani, Susanna, Yerbury, Justin, Clark, Richard, Atkin, Julie, Lee, John, Gordon, Richard and Woodruff, Trent M. (2017). Motor neuron disease proteins activate complement and generate C5a. 16th European Meeting on Complement in Human Disease (EMCHD), Copenhagen, Denmark, 8-12 September 2017. Kidlington, Oxford, United Kingdom: Pergamon Press. doi: 10.1016/j.molimm.2017.06.144

Development of peptide therapeutics for iron disorders

Goncalves, Daniela, Rishi, Gautam, Aliyanto, Randy, Rosengren, Johan, Subramaniam, Nathan, Frazer, David and Clark, Richard (2016). Development of peptide therapeutics for iron disorders. 6th Meeting of the International BioIron Society, Zhejiang University, Hangzhou, China, 8-10 September 2015. Hoboken, NJ, United States: John Wiley & Sons.

Discovery, Synthesis and Characterization of Novel Cysteine-Rich Peptides From Insects

Koehbach, J. and Clark, R. J. (2016). Discovery, Synthesis and Characterization of Novel Cysteine-Rich Peptides From Insects. HOBOKEN: WILEY.

Pharmacological Characterisation of the Cyclotide Kalata B7 At Insect Orthologues of the Oxytocin/vasopressin Receptors

Keov, P., Liutkeviciute, Z., Clark, R. and Gruber, C. (2016). Pharmacological Characterisation of the Cyclotide Kalata B7 At Insect Orthologues of the Oxytocin/vasopressin Receptors. HOBOKEN: WILEY.

Exploring the chemistry and pharmacological potential of bioactive peptides from insects

Koehbach, Johannes and Clark, Richard J. (2015). Exploring the chemistry and pharmacological potential of bioactive peptides from insects. 14th International Congress on Amino Acids, Peptides and Proteins, Vienna, Austria, August 3–7, 2015. Wien, Austria: Springer Wien. doi: 10.1007/s00726-015-2016-z

A new target for the alpha-conotoxins: the gaba(b) receptor

Clark, R. J., Carstens, B. B., Berecki, G., Callaghan, B., Adams, D. J. and Craik, D. J. (2014). A new target for the alpha-conotoxins: the gaba(b) receptor. Oxford, United Kingdom: John Wiley & Sons.

Applications of conotoxins for the treatment of pain

Craik, David J., Clark, Richard J. and Poth, Aaron (2014). Applications of conotoxins for the treatment of pain. 247th National Spring Meeting of the American Chemical Society (ACS), Dallas, TX, United States, March 16-20, 2014. Washington, DC, United States: American Chemical Society.

Development of analgesic alpha-conotoxins for treatment of chronic pain

Adams, D. J., Berecki, G., Clark, R. and Craik, D. J. (2013). Development of analgesic alpha-conotoxins for treatment of chronic pain. 4th Meeting of the Australia Chinese Association for Biomedical Sciences Inc (ACABS), Hangzhou Peoples R China, 10-13 October 2013. Richmond, VIC Australia: Wiley-Blackwell Publishing Asia. doi: 10.1111/1440-1681.12170

alpha-Conotoxin Regiia Targeting Nicotinic Acetylcholine Receptors: Mutagenesis Studies Improving Selectivity and Potency

Kompella, Shiva N., Hung, Andrew, Clark, Richard J. and Adams, David J. (2013). alpha-Conotoxin Regiia Targeting Nicotinic Acetylcholine Receptors: Mutagenesis Studies Improving Selectivity and Potency. 57th Annual Meeting of the Biophysical Society, Philadelphia Pa, 02-06 February 2013. Maryland Heights, MO United States: Cell Press. doi: 10.1016/j.bpj.2012.11.3505

Engineering pro-angiogenic peptides using stable disulfide-rich scaffolds

Chan, L. A., Gunasekera, S., Henriques, S. T., Worth, N. F., Le, S., Clark, R. J., Campbell, J. H., Craik, D. J. and Daly, N. L. (2012). Engineering pro-angiogenic peptides using stable disulfide-rich scaffolds. 32nd European Peptide Symposium, Athens, Greece, 2-7 September 2012. Chichester, West Sussex, United Kingdom: John Wiley & Sons. doi: 10.1002/psc.2448

Novel single-chain ligands for the relaxin-3 receptor RXFP3

Rosengren, K. J., Haugaard-Kedstroem, L. M., Jones, M., Clark, R. J. and Bathgate, R. A. D. (2012). Novel single-chain ligands for the relaxin-3 receptor RXFP3. 32nd European Peptide Symposium "Peptides 2012", Athens, Greece, 02-07 September 2012. West Sussex, United Kingdom: John Wiley and Sons. doi: 10.1002/psc.2448

Understanding the structure/activity relationships of the iron regulatory peptide hepcidin

Clark, R. J., Tan, C., Preza, G. C., Nemeth, E., Ganz, T. and Craik, D. J. (2012). Understanding the structure/activity relationships of the iron regulatory peptide hepcidin. 32nd European Peptide Symposium "Peptides 2012", Athens, Greece, 2-7 September 2012. West Sussex, United Kingdom: John Wiley and Sons. doi: 10.1002/psc.2448

Scanning mutagenesis of alpha-conotoxin AuIB reveals a critical residue for activity at the alpha-3-beta-4 nicotinic acetylcholine receptor

Adams, D. J., Grishin, A. A., Hung, A., Clark, R. J., Akondi, K., Alewood, P. F. and Craik, D. J. (2011). Scanning mutagenesis of alpha-conotoxin AuIB reveals a critical residue for activity at the alpha-3-beta-4 nicotinic acetylcholine receptor. nAChR2011: Nicotinic Acetylcholine Receptors as Therapeutic Targets: Emerging Frontiers in Basic Research & Clinical Science, Washington, DC, U.S.A., 9-11 November 2011. Philadelphia, PA, U.S.A.: Elsevier. doi: 10.1016/j.bcp.2011.07.017

Evaluation of toxicity and antitumor activity of cycloviolacin O2 in mice

Burman, Robert, Svedlund, Erika, Felth, Jenny, Hassan, Saadia, Hermann, Anders, Clark, Richard James, Craik, David J., Bohlin, Lars, Claeson, Per, Goransson, Ulf and Gullbo, Joachim (2010). Evaluation of toxicity and antitumor activity of cycloviolacin O2 in mice. 1st International Conference on Circular Proteins, Heron Island, Qld, Australia, 18-21 October, 2009. Hoboken, NJ, U.S.A: Wiley Interscience. doi: 10.1002/bip.21408

Sunflower trypsin inhibitor-1, proteolytic studies on a trypsin inhibitor peptide and its analogs

Colgrave, Michelle, Korsinczky, Michael Laszlo Jonas, Clark, Richard James, Foley, Fiona and Craik, David J. (2010). Sunflower trypsin inhibitor-1, proteolytic studies on a trypsin inhibitor peptide and its analogs. 1st International Conference on Circular Proteins, Heron Island, Qld, Australia, 18-21 October 2009. Hoboken, N.J., U.S.: John Wiley & Sons. doi: 10.1002/bip.21415

The engineering of an orally active conotoxin for the treatment of neuropathic pain

Clark, J., Jensen, J., Nevin, S., Brid, C., Adams, D. and Craik, D. (2010). The engineering of an orally active conotoxin for the treatment of neuropathic pain. EPS 2010: The 31st European Peptide Symposium, Copenhagen, Denmark, 5-9 September 2010. Bognor Regis, West Sussex, U.K.: John Wiley & Sons. doi: 10.1002/psc.1302

Mutational atudies on alpha-conotoxin VCL1

Halai, R., Clark, R., Nevin, S., Adams, D. and Craik, D. (2009). Mutational atudies on alpha-conotoxin VCL1. 21st American Peptide Symposium, Bloomington, Indiana, USA, 7-12 June 2009. United States: John Wiley & Sons, Inc.. doi: 10.1002/bip.21223

Structural Aspects of Hepcidin-Ferroportin Binding

Preza, G, Fernandes, A, Clark, RJ, Craik, DJ, Ganz, T and Nemeth, E (2008). Structural Aspects of Hepcidin-Ferroportin Binding. 50th Annual Meeting of the American- Society-of-Hematology, San Francisco Ca, Dec 06-09, 2008. WASHINGTON: AMER SOC HEMATOLOGY.

Synthesis and folding of the circular cystine knotted cyclotide cycloviolacin O2

Aboye, Teshome, Clark, Richard, Craik, David J. and Goransson, Ulf (2008). Synthesis and folding of the circular cystine knotted cyclotide cycloviolacin O2. 30th European Peptide Symposium (30EPS), Helsinki, Finland, 31 August – 5 September 2008. United Kingdom: John Wiley & Sons Ltd.. doi: 10.1002/psc.1090

Synthesis and folding of the circular cystine knotted cyclotide cycloviolacin O2

Aboye, Teshome, Clark, Richard, Craik, David J. and Goransson, Ulf (2008). Synthesis and folding of the circular cystine knotted cyclotide cycloviolacin O2. 7th Joint Meeting of GA, AFERP, ASP, PSE & SIF, Athens, Greece, 3-8 August 2008. Germany: Georg Thieme Verlag.

Understanding the structure/activity relationships of hepcidin

Clark, Richard, Tan, Chia Chia, Nemeth, Elizabeta, Ganz, Thomas and Craik, David (2008). Understanding the structure/activity relationships of hepcidin. 30th European Peptide Symposium (30EPS), Helsinki, Finland, 31 August – 5 September 2008. United Kingdom: John Wiley & Sons Ltd.. doi: 10.1002/psc.1090

Enzyme mechanism and function of a novel plant protein disulfide isomerase involved in the oxidative folding of cystine knot defence peptides

Gruber, G. W., Cemazar, M., Clark, R., Horibe, T., Renda, R., Anderson, M. A. and Craik, D. J. (2007). Enzyme mechanism and function of a novel plant protein disulfide isomerase involved in the oxidative folding of cystine knot defence peptides. 20th American-Peptide-Society Symposium, Montréal, Canada, 26-30 Jun 2007. Hoboken, N. J., U.S.: John Wiley & Sons. doi: 10.1002/bip.20783

A novel threaded ring structure for the antimicrobial peptide microcin J25

Rosengren, KJ, Jen, FEC, Clark, RJ, Goransson, U, Daly, NL and Craik, DJ (2005). A novel threaded ring structure for the antimicrobial peptide microcin J25. Peptides 2004: 3rd International Peptide Symposium / 28th European Peptide Symposium, Prague, Czech Republic, 5 - 10 September 2004. Geneva, Switzerland: Kenes International.

A novel threaded ring structure for the antimicrobial peptide microcin J25

Craik, D. J., Rosengren, K. J., Clark, R. J., Daly, N. L. and Goransson, U. (2004). A novel threaded ring structure for the antimicrobial peptide microcin J25. 3rd International and 28th European Peptide Symposium, Prague, Czech Republic, 5–10 September 2004. West Sussex, United Kingdom: John Wiley & Sons. doi: 10.1002/psc.617

Cyclotides: Novel Peptidic Pesticides for Plant Protection

Plan, MR, Goransson, U, Rosengren, KJ, Clark, RJ, Daly, NL, Evert, P, Chen, C, Cagauan, AG and Craik, DJ (2004). Cyclotides: Novel Peptidic Pesticides for Plant Protection. CHICHESTER: JOHN WILEY & SONS LTD.

Design, Synthesis and Structural Studies of Cyclic Alpha-Conotoxins

Clark, RJ, Dempster, L, Daly, NL, Rosengren, KJ and Craik, DJ (2004). Design, Synthesis and Structural Studies of Cyclic Alpha-Conotoxins. CHICHESTER: JOHN WILEY & SONS LTD.

Probing nucleotide dissociation from myosin in vitro using microgram quantities of myosin

Clark, Richard J., Nyitrai, Miklós, Webb, Martin R. and Geeves, Michael A. (2003). Probing nucleotide dissociation from myosin in vitro using microgram quantities of myosin.

Sponge allelochemical induces ascidian development but inhibits metamorphosis

Green, K., Russell, B. D., Clark, R., Jones, M. K., Skilleter, G. A., Garson, M. J. and Degnan, B. M. (2002). Sponge allelochemical induces ascidian development but inhibits metamorphosis. 23rd International Conference on Natural Products, Italy, July, 2002.

Sponge alleleochemical induces ascidian settlement but inhibits metamorphosis

Green, K., Russell, B. D., Clark, R. J., Jones, M. K., Skilleter, G. A., Garson, M. J. and Degnan, B. M. (2001). Sponge alleleochemical induces ascidian settlement but inhibits metamorphosis. 3rd IUPAC International Conference on Biodiversity, Turkey, 2001. Antalya, Turkey:

Sponge allelochemical induces ascidian development but inhibits metamorphosis

Clark, R. J., Degnan, B. M., Garson, M. J., Green, K. M., Jones, M. K., Russell, B. D. and Skilleter, G. A. (2001). Sponge allelochemical induces ascidian development but inhibits metamorphosis. The World Chemistry Congress 2001, Brisbane, Australia, 106 July 2001.

Marine ecology: The natural approach to finding bioactives

Garson, M. J., Blunt, J. W., Clark, R., Degnan, B. M., Green, K. M., Munro, M. H., Simpson, J. and Skilleter, G. A. (2000). Marine ecology: The natural approach to finding bioactives. Australasian Biotechnological Association Conference, Brisbane, 2-6 July, 2000.

Sponge allelochemical induces ascidian development but inhibits metamorphosis

Green, K. M., Russell, B. D., Clark, R., Jones, M. K., Skilleter, G. A., Garson, M. J. and Degnan, B. M. (2000). Sponge allelochemical induces ascidian development but inhibits metamorphosis. Pacifichem 2000, Honolulu, Hawaii, USA, 14-19 December, 2000.

Ecological role of cytotoxic alkaloids: Haliclona n.sp., an unusual sponge/dinoflagellate association

Garson, M. J., Clark, R., Webb, R. I., Field, K. L. and Charan, R. D. (1999). Ecological role of cytotoxic alkaloids: Haliclona n.sp., an unusual sponge/dinoflagellate association. 5th International Sponge Conference, Brisbane, 27 June - 4 July 1999. Brisbane: Qld Museum.

The chemistry and chemical ecology of marine sponges

Clark, Richard James (2000). The chemistry and chemical ecology of marine sponges. PhD Thesis, School of Molecular and Microbial Sciences, The University of Queensland.