Dr Mohammed Shaker

Postdoc Res Fellow in Organoid Biol

Australian Institute for Bioengineering and Nanotechnology
m.shaker@uq.edu.au
+61 7 334 63894

Overview

A neuroscientist, Dr Shaker received his PhD in 2017 from Korea University, Seoul-South Korea, after working on the cellular behaviour of embryonic neural stem cells during the brain and spinal cord development as well as axial elongation. Dr Shaker obtained the Brain Korea 21 Plus Fellowship from the Korea Univesity Medical School before being appointed in AIBN-UQ to work in Professor Ernst Wolvetang's laboratory as a Postdoctoral Research Fellow in Organoid Biology to continue his research into the importance of modelling the human central nervous system in health and disease using organoid technology. Dr Shaker demonstrated:

1- Successful generation of white matter like tissues in organoids, this tissue is enriched with myelinating oligodendrocytes, neurons and astrocytes (Shaker MR et al, Frontiers in Cellular Neuroscience 2021). This protocol is robust and rapid that involves a 42-day exposure of neuroectoderm-derived organoids to a cocktail of growth factors and small molecules that collectively foster oligodendrocyte specification and survival along with neurons and astrocytes.

2- Human cortical organoids develop the typical hallmarks of senescent cells when maintained in vitro for prolonged periods of time, and that moderate upregulation of endogenous KL expression in cortical organoids inhibits neuronal senescence (Shaker MR et al, NPJ Aging and Mechanisms of Disease 2021). This platform offers new mechanistic insight into its role in human brain ageing.

3- Successful generation of the blood-cerebrospinal fluid barrier of the choroid plexus in human organoids (Shaker MR et al, BioRxiv 2020), this protocol is highly reproducible independent of cell lines, clones or batches.

Currently, Dr Shaker is working on three main projects:

1- Modelling leukodystrophy using 4 reprogrammed patients iPSCs lines with an oligodendrocyte brain organoid.

2- Modelling Down Syndrome using patient and isogenic human iPSCs lines with Choroid Plexus-Ventricle-Cortical organoid.

3- Generating spinal cord organoids from iPSCs derived from healthy individuals and MND patients to model human ALS in a dish.

Research Interests

  • Neurogenesis
  • Myelination
  • Ageing
  • Motor Neuron Degeneration
  • Mitochondria

Qualifications

  • Bachelor of Biomedical Science, University of Malaya
  • Master of Medical Science, University of Malaya
  • Doctor of Philosophy, Korea University

Publications

View all Publications

Publications

Journal Article

Conference Publication

Grants (Administered at UQ)