Dr. Lucía F. Zacchi is a molecular and cellular biologist, specialized in antibody discovery, protein quality control, glycobiology, and mass spectrometry proteomics.
She graduated as Microbiologist from Universidad Nacional de Río Cuarto, Argentina. She then obtained a Fulbright Scholarship to pursue her Ph.D. in Microbiology, Immunology, and Cancer Biology at the University of Minnesota, USA. For her dissertation she studied mechanisms of phenotypic variation in the pathogenic yeast Candida albicans. She did post-doctoral work in the laboratories of Dr. Jeffrey Brodsky (University of Pittsburgh, USA), Dr. Julio Caramelo (Fundacion Instituto Leloir, Argentina), Dr. Benjamin L. Schulz (SCMB, UQ), and the ARC Training Centre for Biopharmaceutical Innovation (AIBN, UQ). Dr. Zacchi has been the recipient of Post-doctoral Fellowships from the Dystonia Medical Research Foundation, CONICET-Argentina, Endeavour-Australia, and The University of Queensland.
Globally, Dr Zacchi's research aims to better understand cellular processes that lead to phenotypic variability and to dissect the molecular machinery involved in the secretory pathway, in order to manipulate them for medical and biotechnological applications. She uses the model yeast Saccharomyces cerevisiae (Baker's yeast), plants, and mammalian cells to study mechanisms of protein glycosylation, protein quality control, and protein degradation in the cell, under physiological conditions or during disease, and to produce therapeutic proteins. Her research employs a variety of techniques, including genetics, molecular and cellular biology, and proteomics and she develops new molecular tools, genetic screens, and glycoproteomic workflows. The current focus of Dr Zacchi's work is in developing new antibodies and new antibody technologies.
Dr. Zacchi's research areas include:
1) Antibody discovery, production, and characterization in multiple eukaryotic hosts (mammalian cells, plants, yeasts).
2) Protein folding and glycobiology.
3) Glycoengineering of cell lines and bioprocesses.
The understanding of the processes involved in protein quality control, folding, and glycosylation reaches fields as diverse as microbial pathogenesis and human genetic diseases, and has important biotechnological, clinical, and industrial applications. We are using these knowledge to help us design new tools to generate antibodies with therapeutic and/or diagnostic potential.
Journal Article: TorsinA folding and N-linked glycosylation are sensitive to redox homeostasis
Honer, Jonas, Niemeyer, Katie M., Fercher, Christian, Diez Tissera, Ana L., Jaberolansar, Noushin, Jafrani, Yohaann M. A., Zhou, Chun, Caramelo, Julio J., Shewan, Annette M., Schulz, Benjamin L., Brodsky, Jeffrey L. and Zacchi, Lucía F. (2021). TorsinA folding and N-linked glycosylation are sensitive to redox homeostasis. Biochimica et Biophysica Acta. Molecular Cell Research, 1868 (9) 119073, 119073. doi: 10.1016/j.bbamcr.2021.119073
Journal Article: SWATH-MS glycoproteomics reveals consequences of defects in the glycosylation machinery
Zacchi, Lucia F. and Schulz, Benjamin L. (2016). SWATH-MS glycoproteomics reveals consequences of defects in the glycosylation machinery. Molecular and Cellular Proteomics, 15 (7), 2435-2447. doi: 10.1074/mcp.M115.056366
Journal Article: N-glycoprotein macroheterogeneity: biological implications and proteomic characterization.
Zacchi, Lucia F. and Schulz, Benjamin L. (2015). N-glycoprotein macroheterogeneity: biological implications and proteomic characterization.. Glycoconjugate Journal, 33 (3), 1-18. doi: 10.1007/s10719-015-9641-3
Dr Lucia Zacchi - Maternity Funding (Advance Queensland Women's Academic Fund)
(2017–2018) Queensland Government Advance Queensland Women's Academic Fund
Identifying cellular factors controlling early-onset torsion dystonia
(2016) The Parkinson's & Movement Disorder Foundation
Systems biology-guided cell-line engineering for production of therapeutic proteins with complex post-translational modifications
Doctor Philosophy
(2022) Doctor Philosophy
Messaging with magnesium: Function of yeast Ost3 proteins in glycosylation and magnesium
(2019) Doctor Philosophy
Yeasts, glycoproteomics, microbial pathogenesis, neurological diseases, biopharmaceuticals... take your pick!
We are always looking for enthusiastic, team-oriented, and fast-learning students with a background in Biochemistry, Molecular Biology, Microbiology or related fields, and an interest in learning molecular and cellular biology, glycobiology, models of disease, and/or proteomics. Previous experience in a molecular biology laboratory is required (we may request a reference letter).
IMPORTANT: Please note that if you intend to do a Master's or a PhD you will need to have your own scholarship/fellowship.
Our goal is to provide you with the best experience, both inside and outside the lab, which will contribute to train you for a career in research. An integral part of the experience in our lab will involve active participation in lab meetings and journal club discussions. We will encourage you to present your findings to the group. We will also endeavour to coach you in effective presentation techniques, scientific writing, ethics in research, good laboratory practices, and team-work.
We have plenty of projects available. Contact us!
SWATH-MS glycoproteomics reveals consequences of defects in the glycosylation machinery
Zacchi, Lucia F. and Schulz, Benjamin L. (2016). SWATH-MS glycoproteomics reveals consequences of defects in the glycosylation machinery. Molecular and Cellular Proteomics, 15 (7), 2435-2447. doi: 10.1074/mcp.M115.056366
N-glycoprotein macroheterogeneity: biological implications and proteomic characterization.
Zacchi, Lucia F. and Schulz, Benjamin L. (2015). N-glycoprotein macroheterogeneity: biological implications and proteomic characterization.. Glycoconjugate Journal, 33 (3), 1-18. doi: 10.1007/s10719-015-9641-3
Data-independent acquisition for yeast glycoproteomics
Zacchi, Lucía F. and Schulz, Benjamin L. (2019). Data-independent acquisition for yeast glycoproteomics. Yeast systems biology. (pp. 191-202) New York, NY USA: Humana Press. doi: 10.1007/978-1-4939-9736-7_11
Endoplasmic reticulum-associated degradation and protein quality control
Zacchi, L.F., Caramelo, J.J., McCracken, A.A. and Brodsky, J.L. (2016). Endoplasmic reticulum-associated degradation and protein quality control. Encyclopedia of cell biology. (pp. 596-611) edited by Ralph A. Bradshaw and Philip D. Stahl. Waltham, MA, United States: Academic Press.
TorsinA folding and N-linked glycosylation are sensitive to redox homeostasis
Honer, Jonas, Niemeyer, Katie M., Fercher, Christian, Diez Tissera, Ana L., Jaberolansar, Noushin, Jafrani, Yohaann M. A., Zhou, Chun, Caramelo, Julio J., Shewan, Annette M., Schulz, Benjamin L., Brodsky, Jeffrey L. and Zacchi, Lucía F. (2021). TorsinA folding and N-linked glycosylation are sensitive to redox homeostasis. Biochimica et Biophysica Acta. Molecular Cell Research, 1868 (9) 119073, 119073. doi: 10.1016/j.bbamcr.2021.119073
Rubbab, Tehseen, Pegg, Cassandra L., Phung, Toan K., Nouwens, Amanda S., Yeo, K.Y. Benjamin, Zacchi, Lucia F., Muhammad, Amna, Naqvi, S.M. Saqlan and Schulz, Benjamin L. (2021). N-glycosylation on Oryza sativa root germin-like protein 1 is conserved but not required for stability or activity. Biochemical and Biophysical Research Communications, 553, 72-77. doi: 10.1016/j.bbrc.2021.03.024
Zacchi, Lucia F., Roche-Recinos, Dinora, Pegg, Cassandra L., Phung, Toan K., Napoli, Mark, Aitken, Campbell, Sandford, Vanessa, Mahler, Stephen M., Lee, Yih Yean, Schulz, Benjamin L. and Howard, Christopher B. (2021). Coagulation factor IX analysis in bioreactor cell culture supernatant predicts quality of the purified product. Communications Biology, 4 (1) 390, 390. doi: 10.1038/s42003-021-01903-x
Resolving the TorsinA Oligomerization Conundrum: The Glycan Hypothesis
Fercher, Christian and Zacchi, Lucía F. (2020). Resolving the TorsinA Oligomerization Conundrum: The Glycan Hypothesis. Frontiers in Molecular Biosciences, 7 585643, 585643. doi: 10.3389/fmolb.2020.585643
Identification of novel glycosylation events on human serum-derived factor IX
Pegg, Cassandra L., Zacchi, Lucia F., Recinos, Dinora Roche, Howard, Christopher B. and Schulz, Benjamin L. (2020). Identification of novel glycosylation events on human serum-derived factor IX. Glycoconjugate Journal, 37 (4), 471-483. doi: 10.1007/s10719-020-09922-2
Glycoproteomic measurement of site-specific polysialylation
Pelingon, Ruby, Pegg, Cassandra L., Zacchi, Lucia F., Phung, Toan K., Howard, Christopher B., Xu, Ping, Hardy, Matthew P., Owczarek, Catherine M. and Schulz, Benjamin L. (2020). Glycoproteomic measurement of site-specific polysialylation. Analytical Biochemistry, 596 113625, 113625. doi: 10.1016/j.ab.2020.113625
Zacchi, Lucia F., Roche Recinos, Dinora, Otte, Ellen, Aitken, Campbell, Hunt, Tony, Sandford, Vanessa, Lee, Yih Yean, Schulz, Benjamin L. and Howard, Christopher B. (2020). S-trap eliminates cell culture media polymeric surfactants for effective proteomic analysis of mammalian cell bioreactor supernatants. Journal of Proteome Research, 19 (5), 2149-2158. doi: 10.1021/acs.jproteome.0c00106
Phung, Toan K., Zacchi, Lucia F. and Schulz, Benjamin L. (2020). DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides. Molecular Omics, 16 (2), 100-112. doi: 10.1039/c9mo00125e
Fortes, M. R.S., Zacchi, L. F., Nguyen, L. T., Raidan, F., Weller, M. M.D.C.A., Choo, J. J.Y., Reverter, A., Rego, J. P.A., Boe-Hansen, G. B., Porto-Neto, L. R., Lehnert, S. A., Cánovas, A., Schulz, B. L., Islas-Trejo, A., Medrano, J. F., Thomas, M. G. and Moore, S. S. (2018). Pre- and post-puberty expression of genes and proteins in the uterus of Bos indicus heifers: the luteal phase effect post-puberty. Animal Genetics, 49 (6), 539-549. doi: 10.1111/age.12721
Adipose tissue proteomic analyses to study puberty in Brahman heifers
Nguyen, L. T., Zacchi, L. F., Schulz, B. L., Moore, S. S. and Fortes, M. R. S. (2018). Adipose tissue proteomic analyses to study puberty in Brahman heifers. Journal of Animal Science, 96 (6), 2392-2398. doi: 10.1093/jas/sky128
Zacchi, Lucia F., Dittmar, John C., Mihalevic, Michael J., Shewan, Annette M., Schulz, Benjamin L., Brodsky, Jeffrey L. and Bernstein, Kara A. (2017). Early-onset torsion dystonia: a novel high-throughput yeast genetic screen for factors modifying protein levels of torsinA Delta E. Disease Models & Mechanisms, 10 (9), 1129-1140. doi: 10.1242/dmm.029926
N-glycosylation triggers a dual selection pressure in eukaryotic secretory proteins
Medus, Maximo L., Gomez, Gabriela E., Zacchi, Lucia F., Couto, Paula M., Labriola, Carlos A., Labanda, Maria S., Bielsa, Rodrigo Corti, Clérico, Eugenia M., Schulz, Benjamin L. and Caramelo, Julio J. (2017). N-glycosylation triggers a dual selection pressure in eukaryotic secretory proteins. Scientific Reports, 7 (1) 8788, 8788. doi: 10.1038/s41598-017-09173-6
SWATH-MS glycoproteomics reveals consequences of defects in the glycosylation machinery
Zacchi, Lucia F. and Schulz, Benjamin L. (2016). SWATH-MS glycoproteomics reveals consequences of defects in the glycosylation machinery. Molecular and Cellular Proteomics, 15 (7), 2435-2447. doi: 10.1074/mcp.M115.056366
N-glycoprotein macroheterogeneity: biological implications and proteomic characterization.
Zacchi, Lucia F. and Schulz, Benjamin L. (2015). N-glycoprotein macroheterogeneity: biological implications and proteomic characterization.. Glycoconjugate Journal, 33 (3), 1-18. doi: 10.1007/s10719-015-9641-3
Erratum to: Biocontrol and PGPR features in native strains isolated from saline soils of Argentina
Principe, Analia, Alvarez, Florencia, Castro, Marina G., Zacchi, Lucia F., Fischer, Sonia E., Mori, Gladys B. and Jofre, Edgardo (2014). Erratum to: Biocontrol and PGPR features in native strains isolated from saline soils of Argentina. Current Microbiology, 69 (3), 404-404. doi: 10.1007/s00284-014-0601-0
Intracellular Complexes of the Early-Onset Torsion Dystonia-Associated AAA+ ATPase TorsinA
Li, Hui, Wu, Hui-Chuan, Liu, Zhonghua, Zacchi, Lucia F., Brodsky, Jeffrey L. and Zolkiewski, Michal (2014). Intracellular Complexes of the Early-Onset Torsion Dystonia-Associated AAA+ ATPase TorsinA. SpringerPlus, 3 (1) 743, 743.1-743.5. doi: 10.1186/2193-1801-3-743
Zacchi, Lucía F., Wu, Hui-Chuan, Bell, Samantha L., Millen, Linda, Paton, Adrienne W., Paton, James C., Thomas, Philip J., Zolkiewski, Michal and Brodsky, Jeffrey L. (2014). The BiP molecular chaperone plays multiple roles during the biogenesis of TorsinA, an AAA+ ATPase associated with the neurological disease Early-Onset Torsion Dystonia. Journal of Biological Chemistry, 289 (18), 12727-12747. doi: 10.1074/jbc.M113.529123
Gerami-Nejad, Maryam, Zacchi, Lucia F., McClellan, Mark, Matter, Kathleen and Berman, Judith (2013). Shuttle vectors for facile cloning through gap repair and integration into a neutral locus in Candida albicans. Microbiology, 159 (Part 3), 565-579. doi: 10.1099/mic.0.064097-0
HOS2 and HDA1 encode histone deacetylases with opposing roles in Candida albicans morphogenesis
Zacchi, Lucia F., Schulz, Wade L. and Davis, Dana A. (2010). HOS2 and HDA1 encode histone deacetylases with opposing roles in Candida albicans morphogenesis. PLoS One, 5 (8), e12171.1-e12171.7. doi: 10.1371/journal.pone.0012171
Low dosage of histone H4 leads to growth defects and morphological changes in Candida albicans
Zacchi, Lucia F., Selmecki, Anna M., Berman, Judith and Davis, Dana A. (2010). Low dosage of histone H4 leads to growth defects and morphological changes in Candida albicans. PLoS One, 5 (5), e10629.1-e10629.12. doi: 10.1371/journal.pone.0010629
Mds3 regulates morphogenesis in Candida albicans through the TOR pathway
Zacchi, Lucia F., Gomez-Raja, Jonathan and Davis, Dana A. (2010). Mds3 regulates morphogenesis in Candida albicans through the TOR pathway. Molecular and Cellular Biology, 30 (14), 3695-3710. doi: 10.1128/MCB.01540-09
Biocontrol and PGPR features in native strains isolated from saline soils of Argentina
Principe, Analia, Alvarez, Florencia, Castro, Marina G., Zacchi, Lucia, Fischer, Sonia E., Mori, Gladys B. and Jofre, Edgardo (2007). Biocontrol and PGPR features in native strains isolated from saline soils of Argentina. Current Microbiology, 55 (4), 314-322. doi: 10.1007/s00284-006-0654-9
Zacchi, Lucia Florencia, Wu, Hui-Chuan, Niemeyer, Katie, Bell, Samantha, Zolkiewski, Michal and Brodsky, Jeffrey (2012). The ER chaperones BiP and Jem1/Scj1 are genetic modifiers of TorsinA, the AAA plus ATPase associated with the neurological disease primary torsion dystonia. Experimental Biology Meeting, San Diego, CA, United States, 21-25 April 2012. Hoboken, NJ, United States: John Wiley & Sons.
Dr Lucia Zacchi - Maternity Funding (Advance Queensland Women's Academic Fund)
(2017–2018) Queensland Government Advance Queensland Women's Academic Fund
Identifying cellular factors controlling early-onset torsion dystonia
(2016) The Parkinson's & Movement Disorder Foundation
Systems biology-guided cell-line engineering for production of therapeutic proteins with complex post-translational modifications
Doctor Philosophy — Associate Advisor
(2022) Doctor Philosophy — Associate Advisor
Messaging with magnesium: Function of yeast Ost3 proteins in glycosylation and magnesium
(2019) Doctor Philosophy — Associate Advisor
Other advisors:
Note for students: The possible research projects listed on this page may not be comprehensive or up to date. Always feel free to contact the staff for more information, and also with your own research ideas.
Yeasts, glycoproteomics, microbial pathogenesis, neurological diseases, biopharmaceuticals... take your pick!
We are always looking for enthusiastic, team-oriented, and fast-learning students with a background in Biochemistry, Molecular Biology, Microbiology or related fields, and an interest in learning molecular and cellular biology, glycobiology, models of disease, and/or proteomics. Previous experience in a molecular biology laboratory is required (we may request a reference letter).
IMPORTANT: Please note that if you intend to do a Master's or a PhD you will need to have your own scholarship/fellowship.
Our goal is to provide you with the best experience, both inside and outside the lab, which will contribute to train you for a career in research. An integral part of the experience in our lab will involve active participation in lab meetings and journal club discussions. We will encourage you to present your findings to the group. We will also endeavour to coach you in effective presentation techniques, scientific writing, ethics in research, good laboratory practices, and team-work.
We have plenty of projects available. Contact us!