The University of Queensland

Protocol for generating embedding-free brain organoids enriched with oligodendrocytes

Al-mhanawi, Bahaa, Marti, Marta Boira, Morrison, Sean D., Gupta, Pallavi, Alani, Maath, Noakes, Peter G., Wolvetang, Ernst J. and Shaker, Mohammed R. (2023). Protocol for generating embedding-free brain organoids enriched with oligodendrocytes. STAR Protocols, 4 (4) 102725, 102725. doi: 10.1016/j.xpro.2023.102725

Transcriptional Signature of Valproic Acid-Induced Neural Tube Defects in Human Spinal Cord Organoids

Lee, Ju-Hyun, Shaker, Mohammed R., Park, Si-Hyung and Sun, Woong (2023). Transcriptional Signature of Valproic Acid-Induced Neural Tube Defects in Human Spinal Cord Organoids. International Journal of Stem Cells, 16 (4), 385-393. doi: 10.15283/ijsc23012

Choroid plexus defects in Down syndrome brain organoids enhance neurotropism of SARS-CoV-2

Shaker, Mohammed R., Slonchak, Andrii, Al-mhanawi, Bahaa, Morrison, Sean D., Sng, Julian D. J., Cooper-White, Justin, Khromykh, Alexander A. and Wolvetang, Ernst J. (2023). Choroid plexus defects in Down syndrome brain organoids enhance neurotropism of SARS-CoV-2. bioRxiv. doi: 10.1101/2023.06.12.544552

Robust and highly reproducible generation of cortical brain organoids for modelling brain neuronal senescence in vitro

Shaker, Mohammed R., Hunter, Zoe L. and Wolvetang, Ernst J. (2022). Robust and highly reproducible generation of cortical brain organoids for modelling brain neuronal senescence in vitro. Journal of Visualized Experiments, 2022 (183) e63714. doi: 10.3791/63714

Human induced pluripotent stem cells generated from epilepsy patients for use as in vitro models for drug screening

Hunter, Zoe L., Leeson, Hannah C., Shaker, Mohammed R., Wolvetang, Ernst J. and Vadlamudi, Lata (2022). Human induced pluripotent stem cells generated from epilepsy patients for use as in vitro models for drug screening. Stem Cell Research, 60 102673, 102673. doi: 10.1016/j.scr.2022.102673

Production of human spinal-cord organoids recapitulating neural-tube morphogenesis

Lee, Ju-Hyun, Shin, Hyogeun, Shaker, Mohammed R., Kim, Hyun Jung, Park, Si-Hyung, Kim, June Hoan, Lee, Namwon, Kang, Minjin, Cho, Subin, Kwak, Tae Hwan, Kim, Jong Woon, Song, Mi-Ryoung, Kwon, Seung-Hae, Han, Dong Wook, Lee, Sanghyuk, Choi, Se-Young, Rhyu, Im Joo, Kim, Hyun, Geum, Dongho, Cho, Il-Joo and Sun, Woong (2022). Production of human spinal-cord organoids recapitulating neural-tube morphogenesis. Nature Biomedical Engineering, 6 (4), 435-448. doi: 10.1038/s41551-022-00868-4

Neural epidermal growth factor-like like protein 2 is expressed in human oligodendroglial cell types

Shaker, Mohammed R., Kahtan, Amna, Prasad, Renuka, Lee, Ju-Hyun, Pietrogrande, Giovanni, Leeson, Hannah C., Sun, Woong, Wolvetang, Ernst J. and Slonchak, Andrii (2022). Neural epidermal growth factor-like like protein 2 is expressed in human oligodendroglial cell types. Frontiers in Cell and Developmental Biology, 10 803061, 803061. doi: 10.3389/fcell.2022.803061

Hypermethylation of Mest promoter causes aberrant Wnt signaling in patients with Alzheimer's disease

Prasad, Renuka, Jung, Hwajin, Tan, Anderson, Song, Yonghee, Moon, Sungho, Shaker, Mohammed R., Sun, Woong, Lee, Junghee, Ryu, Hoon, Lim, Hyun Kook and Jho, Eek-hoon (2021). Hypermethylation of Mest promoter causes aberrant Wnt signaling in patients with Alzheimer's disease. Scientific Reports, 11 (1) 20075, 20075. doi: 10.1038/s41598-021-99562-9

Generation of induced pluripotent stem cell lines from peripheral blood mononuclear cells of three drug resistant and three drug responsive epilepsy patients

Hunter, Zoe L., Leeson, Hannah C., Shaker, Mohammed R., Wolvetang, Ernst J. and Vadlamudi, Lata (2021). Generation of induced pluripotent stem cell lines from peripheral blood mononuclear cells of three drug resistant and three drug responsive epilepsy patients. Stem Cell Research, 56 102564, 102564. doi: 10.1016/j.scr.2021.102564

Spatiotemporal contribution of neuromesodermal progenitor-derived neural cells in the elongation of developing mouse spinal cord

Shaker, Mohammed R., Lee, Ju-Hyun, Kim, Kyung Hyun, Ban, Saeli, Kim, Veronica Jihyun, Kim, Joo Yeon, Lee, Ji Yeoun and Sun, Woong (2021). Spatiotemporal contribution of neuromesodermal progenitor-derived neural cells in the elongation of developing mouse spinal cord. Life Sciences, 282 119393, 1-11. doi: 10.1016/j.lfs.2021.119393

Inhibition of the cGAS‐STING pathway ameliorates the premature senescence hallmarks of Ataxia‐Telangiectasia brain organoids

Aguado, Julio, Chaggar, Harman K., Gómez‐Inclán, Cecilia, Shaker, Mohammed R., Leeson, Hannah C., Mackay‐Sim, Alan and Wolvetang, Ernst J. (2021). Inhibition of the cGAS‐STING pathway ameliorates the premature senescence hallmarks of Ataxia‐Telangiectasia brain organoids. Aging Cell, 20 (9) e13468, 1-16. doi: 10.1111/acel.13468

Klotho inhibits neuronal senescence in human brain organoids

Shaker, Mohammed R., Aguado, Julio, Chaggar, Harman Kaur and Wolvetang, Ernst J. (2021). Klotho inhibits neuronal senescence in human brain organoids. npj Aging and Mechanisms of Disease, 7 (1) 18, 1-12. doi: 10.1038/s41514-021-00070-x

Embryonal neuromesodermal progenitors for caudal central nervous system and tissue development

Shaker, Mohammed R., Lee, Ju-Hyun and Sun, Woong (2021). Embryonal neuromesodermal progenitors for caudal central nervous system and tissue development. Journal of Korean Neurosurgical Society, 64 (3), 359-366. doi: 10.3340/jkns.2020.0359

Rapid and efficient generation of myelinating human oligodendrocytes in organoids

Shaker, Mohammed R., Pietrogrande, Giovanni, Martin, Sally, Lee, Ju-Hyun, Woong, Sun and Wolvetang, Ernst J. (2021). Rapid and efficient generation of myelinating human oligodendrocytes in organoids. Frontiers in Cellular Neuroscience, 15 631548, 1-11. doi: 10.3389/fncel.2021.631548

Human spinal cord organoids exhibiting neural tube morphogenesis for a quantifiable drug screening system of neural tube defects

Lee, Ju-Hyun, Shin, Hyogeun, Shaker, Mohammed R., Kim, Hyun Jung, Kim, June Hoan, Lee, Namwon, Kang, Minjin, Cho, Subin, Kwak, Tae Hwan and Sun, Woong (2020). Human spinal cord organoids exhibiting neural tube morphogenesis for a quantifiable drug screening system of neural tube defects.

Anteroposterior Wnt-RA gradient defines adhesion and migration properties of neural progenitors in developing spinal cord

Shaker, Mohammed R., Lee, Ju-Hyun, Park, Si-Hyung, Kim, Joo Yeon, Son, Gi Hoon, Son, Jong Wan, Park, Bae Ho, Rhyu, Im Joo, Kim, Hyun and Sun, Woong (2020). Anteroposterior Wnt-RA gradient defines adhesion and migration properties of neural progenitors in developing spinal cord. Stem Cell Reports, 15 (4), 898-911. doi: 10.1016/j.stemcr.2020.08.016

Self-organizing 3D human choroid plexus-ventricle-cortical organoids

Shaker, Mohammed R. , Cooper-White, Justin and Wolvetang, Ernst J. (2020). Self-organizing 3D human choroid plexus-ventricle-cortical organoids.

Spatiotemporal contribution of neuromesodermal progenitor-derived neural cells in the elongation of developing mouse spinal cord

Shaker, Mohammed R. , Lee, Ju-Hyun , Kim, Kyung Hyun , Kim, Veronica Jihyun , Kim, Joo Yeon , Lee, Ji Yeoun and Sun, Woong (2020). Spatiotemporal contribution of neuromesodermal progenitor-derived neural cells in the elongation of developing mouse spinal cord.

NeuroCore formation during differentiation of neurospheres of mouse embryonic neural stem cells

Lee, Ju-Hyun, Shaker, Mohammed R., Lee, Eunsoo, Lee, Boram and Sun, Woong (2020). NeuroCore formation during differentiation of neurospheres of mouse embryonic neural stem cells. Stem Cell Research, 43 101691, 101691. doi: 10.1016/j.scr.2019.101691

High-performance acellular tissue scaffold combined with hydrogel polymers for regenerative medicine

Lee, Eunsoo, Kim, Hyun Jung, Shaker, Mohammed R., Ryu, Jae Ryun, Ham, Min Seok, Seo, Soo Hong, Kim, Dai Hyun, Lee, Kiwon, Jung, Neoncheol, Choe, Youngshik, Son, Gi Hoon, Rhyu, Im Joo, Kim, Hyun and Sun, Woong (2019). High-performance acellular tissue scaffold combined with hydrogel polymers for regenerative medicine. Acs Biomaterials Science and Engineering, 5 (7), 3462-3474. doi: 10.1021/acsbiomaterials.9b00219

Micropillar-based microfluidic device to regulate neurite networks of uniform-sized neurospheres

Kim, Da Eun, Lee, Jong Min, Ahrberg, Christian D., Shaker, Mohammed R., Lee, Ju-Hyun, Sun, Woong and Chung, Bong Geun (2018). Micropillar-based microfluidic device to regulate neurite networks of uniform-sized neurospheres. Electrophoresis, 40 (3), 419-424. doi: 10.1002/elps.201800119

Uniform-sized neurosphere-mediated motoneuron differentiation in microwell arrays

Lee, Jong Min, Moon, Joo Yoon, Shaker, Mohammed R., Sun, Woong and Chung, Bong Geun (2017). Uniform-sized neurosphere-mediated motoneuron differentiation in microwell arrays. Electrophoresis, 38 (24), 3161-3167. doi: 10.1002/elps.201700118

Maternal intake of dietary virgin coconut oil modifies essential fatty acids and causes low body weight and spiky fur in mice

Gunasekaran, Renuka, Shaker, Mohammed Rafid, Mohd-Zin, Siti Waheeda, Abdullah, Aminah, Ahmad-Annuar, Azlina and Abdul-Aziz, Noraishah Mydin (2017). Maternal intake of dietary virgin coconut oil modifies essential fatty acids and causes low body weight and spiky fur in mice. BMC Complementary and Alternative Medicine, 17 (1) 79. doi: 10.1186/s12906-017-1600-z

Identification of molecular markers distinguishing adult neural stem cells in the subventricular and subcallosal zones

Kim, Joo Yeon, Shaker, Mohammed R., Lee, Ju-Hyun, Lee, Boram, Kim, Hyun and Sun, Woong (2017). Identification of molecular markers distinguishing adult neural stem cells in the subventricular and subcallosal zones. Animal Cells and Systems, 21 (3), 152-159. doi: 10.1080/19768354.2017.1324522

Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module

Yim, Nambin, Ryu, Seung-Wook, Choi, Kyungsun, Lee, Kwang Ryeol, Lee, Seunghee, Choi, Hojun, Kim, Jeongjin, Shaker, Mohammed R., Sun, Woong, Park, Ji-Ho, Kim, Daesoo, Heo, Won Do and Choi, Chulhee (2016). Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module. Nature Communications, 7 (1) 12277, 12277. doi: 10.1038/ncomms12277

Promotion of Cortical Neurogenesis from the Neural Stem Cells in the Adult Mouse Subcallosal Zone

Kim, Joo Yeon, Choi, Kyuhyun, Shaker, Mohammed R., Lee, Ju-Hyun, Lee, Boram, Lee, Eunsoo, Park, Jae-Yong, Lim, Mi-Sun, Park, Chang-Hwan, Shin, Ki Soon, Kim, Hyun, Geum, Dongho and Sun, Woong (2016). Promotion of Cortical Neurogenesis from the Neural Stem Cells in the Adult Mouse Subcallosal Zone. Stem Cells, 34 (4), 888-901. doi: 10.1002/stem.2276

Dynamin-related protein 1 controls the migration and neuronal differentiation of subventricular zone-derived neural progenitor cells

Kim, Hyun Jung, Shaker, Mohammed R., Cho, Bongki, Cho, Hyo Min, Kim, Hyun, Kim, Joo Yeon and Sun, Woong (2015). Dynamin-related protein 1 controls the migration and neuronal differentiation of subventricular zone-derived neural progenitor cells. Scientific Reports, 5 (1) 15962. doi: 10.1038/srep15962

Identification and Characterization of Secondary Neural Tube-Derived Embryonic Neural Stem Cells In Vitro

Shaker, Mohammed R., Kim, Joo Yeon, Kim, Hyun and Sun, Woong (2015). Identification and Characterization of Secondary Neural Tube-Derived Embryonic Neural Stem Cells In Vitro. Stem Cells and Development, 24 (10), 1171-1181. doi: 10.1089/scd.2014.0506

Is There a Risk of Suburban Transmission of Malaria in Selangor, Malaysia?

Braima, Kamil A., Sum, Jia-Siang, Ghazali, Amir-Ridhwan M., Muslimin, Mustakiza, Jeffery, John, Lee, Wenn-Chyau, Shaker, Mohammed R., Elamin, Alaa-Eldeen M., Jamaiah, Ibrahim, Lau, Yee-Ling, Rohela, Mahmud, Kamarulzaman, Adeeba, Sitam, Frankie, Mohd-Noh, Rosnida and Abdul-Aziz, Noraishah M. (2013). Is There a Risk of Suburban Transmission of Malaria in Selangor, Malaysia?. PL o S One, 8 (10) e77924, e77924. doi: 10.1371/journal.pone.0077924

Chemogenetic modulation of human in vitro motoneuron development accelerates maturation trajectory and electrophysical properties

Morrison, Sean, Shaker, Mohammed, Pietrogrande, Giovanni, Wolvetang, Ernst and Noakes, Peter (2023). Chemogenetic modulation of human in vitro motoneuron development accelerates maturation trajectory and electrophysical properties. ISN‐ESN 2023 Meeting, Porto, Portugal, 8‐11 August 2023. Chichester, West Sussex, United Kingdom: Wiley-Blackwell.

Human neural organoid model exhibiting early neural tube morphogenesis

Lee, Ju-Hyun, Shaker, Mohammed R., Kim, Hyun Jung, Kim, June Hoan, Shin, Hyogeun, Kang, Minjin, Kwak, Tae Hwan, Rhyu, Im Joo, Kim, Hyun, Han, Dong Wook, Cho, Il-Joo, Geum, Dongho and Sun, Woong (2019). Human neural organoid model exhibiting early neural tube morphogenesis. IBRO World Congress, Daegu, South Korea, 21-25 September 2019. Amsterdam, Netherlands: Elsevier BV. doi: 10.1016/j.ibror.2019.07.678

Lumican is required for axial neural stem cells adhesion gradient and proper spinal cord elongation

Shaker, M. (2019). Lumican is required for axial neural stem cells adhesion gradient and proper spinal cord elongation. ISN-ASN Meeting, Montreal, Canada, 4-8 August 2019. Chichester, West Sussex, United Kingdom: Wiley-Blackwell.

Adhesive property of neuromesodermal progenitor-derived neural stem cells is regulated by wnt signalling

Shaker, M., Lee, J. -H. and Sun, W. (2017). Adhesive property of neuromesodermal progenitor-derived neural stem cells is regulated by wnt signalling. ISN-ESN Meeting, Paris, France, 20-24 August, 2017. Chichester, West Sussex, United Kingdom: Wiley-Blackwell Publishing.